SSGCID: Target Page

TrbrA.00088.a

aspartate carbamoyltransferase, putative

CENTER ID:	TrbrA.00088.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.00088.a.B1.GE34475	full length		1	327

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.5.3820
RefSeq:	XP_845052.1
UniProt:	Q57U84

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAELQPVTSL KGKSIITAAQ FTRPDIDALI RLATALKEKI CAGEVLRFLE GRIMTPLFFE DSSRTLNSFC AAMARLGGRV VYFKAETSSV NKGETLGDTV RTLDSYSDVL VLRHPKQEAI TEAVAKATHP VLNAGNGAGE HPTQALLDVL TIHSELGRVD GSTIAMIGDL KMGRTVHSLL KLLVRNFKMK TIFFVAPDAL QMPQDVVDSL KQEIAASGVT IRSSNSLTEE ILGQCDVLYA TRLQKERFAA AALDEAKALQ AFEAAKADIV INAERMKKAK AKMIVMHPLP RNDELCTSVD EDPRAAYFRQ MQYGMYMRMA ILYSVLA

NT Sequence

atggcggagc tgcaacctgt aactagtttg aaggggaaga gcataataac cgcagctcag ttcacccgcc ccgatattga tgctctcatt cgccttgcca ctgcacttaa agagaagata tgtgcgggag aggtgttacg ttttcttgag ggtcgcatca tgacacctct attctttgag gattcctccc gcacgcttaa ttccttttgt gcggccatgg cgaggcttgg aggtcgcgtt gtgtacttta aagccgaaac ctcctccgtg aacaaaggcg agacactcgg ggatacggtt cggacgctgg attcctacag tgacgtactt gtgctgcgtc acccaaagca ggaggccatt acggaagcgg ttgccaaagc tacgcatccc gttttgaatg ccgggaatgg ggccggtgag cacccaacac aagcacttct cgacgtgtta acaatacatt ctgaactggg ccgcgttgac ggtagcacaa tcgcgatgat tggtgatctt aagatgggac gtacggtcca ctcactactg aagttgctgg tgcgtaactt taaaatgaaa accatcttct ttgttgcgcc ggatgcactt caaatgccac aggatgtggt ggactcacta aagcaggaaa tagcagcaag tggcgtgaca attcgaagta gcaactcgct gacagaagaa attttgggac aatgcgatgt gttgtacgcc acccgtctgc agaaggagcg ctttgccgca gcggctctgg acgaagcgaa ggcgttacag gcatttgaag cggcaaaggc ggatatcgtt attaatgccg agcgcatgaa gaaggcgaag gcgaagatga ttgtcatgca cccacttccc cgtaatgatg aattgtgcac cagtgtggac gaggacccac gcgcggcata ctttaggcag atgcagtacg gtatgtatat gcggatggcc atactttaca gtgttctcgc ttaa

Details for TrbrA.00088.a.B1.GE34475

HARVESTED ON:	2/6/2012
SEQUENCED ON:	2/21/2012
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

ttactcnctt aagcgagaac actgtaaagt atggccatcc gcatatacat accgtactgc atctgcctaa agtatgccgc gcgtgggtcc tcgtccacac tggtgcacaa ttcatcatta cggggaagtg ggtgcatgac aatcattttc gccttcgcct tcttcatgcg ctcggcatta ataacgatat ccgcctttgc cgcttcaaat gcctgtaacg ccttcgcttc gtccagagcc gctgcggcaa agcgctcctt ctgcagacgg gtggcgtaca acacatcgca ttgtcccaaa atttcttctg tcagcgagtt gctacttcga attgtcacgc cacttgctgc tatttcctgc tttagtgagt ccaccacatc ctgtggcatt tgaagtgcat ccggcgcaac aaagaagatg gttttcattt taaagttacg caccagcaac ttcagtagtg agtggaccgt acgtcccatc ttaagatcac caatcatcgc gattgtgcta ccgtcaacgc ggcccagttc agaatgtatt gttaacacat cgagaagtgc ttgtgttgga tgctcaccgg ccccattccc ggcattcaaa acgggatgcg tagctttggc aaccgcttcc gtaatggcct cctgctttgg gtgacgcagc acaagtacgt cactgtagga atccagcgta cgaaccgtat ccccgagtgt ctcgcctttg ttcacggagg aggtttcggc tttaaagtac acaacgcgac ctccaagcct cgccatggcc gcacaaaaag aattaagcgt gcgggaggaa tcctcaaaga atagaggtgt catgatgcga ccctcaagaa aacgtaacac ctctcctgca catatcttct ccttcagtgc agtggcaagg cgaatgagag catcaatatc ggggcgggtg aactgagctg cggttattat gctcttcccc ttcaaactag ttacaggttg cagctccgcc atatg

Expected Protein Sequence

MAHHHHHHMA ELQPVTSLKG KSIITAAQFT RPDIDALIRL ATALKEKICA GEVLRFLEGR IMTPLFFEDS SRTLNSFCAA MARLGGRVVY FKAETSSVNK GETLGDTVRT LDSYSDVLVL RHPKQEAITE AVAKATHPVL NAGNGAGEHP TQALLDVLTI HSELGRVDGS TIAMIGDLKM GRTVHSLLKL LVRNFKMKTI FFVAPDALQM PQDVVDSLKQ EIAASGVTIR SSNSLTEEIL GQCDVLYATR LQKERFAAAA LDEAKALQAF EAAKADIVIN AERMKKAKAK MIVMHPLPRN DELCTSVDED PRAAYFRQMQ YGMYMRMAIL YSVLA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg agctgcaacc tgtaactagt ttgaagggga agagcataat aaccgcagct cagttcaccc gccccgatat tgatgctctc attcgccttg ccactgcact taaagagaag atatgtgcgg gagaggtgtt acgttttctt gagggtcgca tcatgacacc tctattcttt gaggattcct cccgcacgct taattccttt tgtgcggcca tggcgaggct tggaggtcgc gttgtgtact ttaaagccga aacctcctcc gtgaacaaag gcgagacact cggggatacg gttcggacgc tggattccta cagtgacgta cttgtgctgc gtcacccaaa gcaggaggcc attacggaag cggttgccaa agctacgcat cccgttttga atgccgggaa tggggccggt gagcacccaa cacaagcact tctcgacgtg ttaacaatac attctgaact gggccgcgtt gacggtagca caatcgcgat gattggtgat cttaagatgg gacgtacggt ccactcacta ctgaagttgc tggtgcgtaa ctttaaaatg aaaaccatct tctttgttgc gccggatgca cttcaaatgc cacaggatgt ggtggactca ctaaagcagg aaatagcagc aagtggcgtg acaattcgaa gtagcaactc gctgacagaa gaaattttgg gacaatgcga tgtgttgtac gccacccgtc tgcagaagga gcgctttgcc gcagcggctc tggacgaagc gaaggcgtta caggcatttg aagcggcaaa ggcggatatc gttattaatg ccgagcgcat gaagaaggcg aaggcgaaga tgattgtcat gcacccactt ccccgtaatg atgaattgtg caccagtgtg gacgaggacc cacgcgcggc atactttagg cagatgcagt acggtatgta tatgcggatg gccatacttt acagtgttct cgcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat