SSGCID: Target Page

TrbrA.00005.a

Mitochondrial malate dehydrogenase

CENTER ID:	TrbrA.00005.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.00005.a.B2.GE34476	mature protein		21	318

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.10.2560

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRTSATLQFK VAVLGAAGGI GQPLSLILKT NPLVSHLSCY DIHGVTGVAA DLSHICSPAK VTGHLKDELH KAVDGADVVI IPAGTPRKPG MTREDLFSVN ATIVRDLVSA CAKQCPKALI GVVSNPVNSV VPIASEVLKK AGVFDPARLF GITTLDVVRA RTFVAEAAGK SPYDVNVQVV GGHSGPTIIP LLSQAGVSLT EEQVKAITRR VQYGGDEVVK AKGGAGSATL SMAYAAGEWM SSVLKGLRGD KGIVECTYVQ TDIIPGVNFF GCPVELGKGG VEKIHKPVFD AYEQSLLEKC VADLEKNIAA GFAFGSRQ

NT Sequence

atgcgtacca gtgcaactct ccagtttaaa gttgccgtcc tcggtgctgc cggtggtatt ggtcaaccgt tatcgcttat cctgaaaacc aatcctcttg tgtctcactt gagttgttat gacatacatg gtgtgacagg cgtggctgca gacctctcgc acatttgttc tccagctaag gtgactggtc acctgaaaga tgaacttcac aaagcagttg acggagctga cgttgtcatc atccccgcgg gcacaccgcg aaagccgggg atgactcgcg aagatctctt cagtgttaat gcaacgatcg ttcgagacct cgtatcggct tgtgcaaaac aatgccccaa ggctcttatc ggtgttgttt ccaaccctgt caacagtgtt gtacctattg cttcggaggt gctcaaaaag gctggtgtct ttgaccctgc tcgcctcttt ggcataacca cgcttgatgt ggtgagagct cgaacatttg tggctgaggc cgctggtaag agtccgtatg acgttaatgt tcaggtggtg ggtggacaca gtggtccgac tattattcca ttattatctc aagctggtgt atcattgacg gaggaacaag tcaaggctat aacgcgacga gtgcagtatg gcggtgacga agttgtgaaa gctaaaggag gggcgggttc agcaactctt tcaatggcgt acgccgctgg ggaatggatg tcctccgtgt tgaaagggct tcgtggtgac aagggtatcg tggagtgtac gtatgttcag acggatatta ttcccggcgt gaactttttc ggttgcccgg tggaacttgg gaaaggtggc gttgaaaaga ttcacaaacc tgtgtttgac gcgtatgaac agagtctttt ggagaaatgt gttgcggact tggagaaaaa tatcgcagca ggttttgcct tcggttccag acagtga

Details for TrbrA.00005.a.B2.GE34476

HARVESTED ON:	2/6/2012
SEQUENCED ON:	2/21/2012
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG QPLSLILKTN PLVSHLSCYD IHGVTGVAAD LSHICSPAKV TGHLKDELHK AVDGADVVII PAGTPRKPGM TREDLFSVNA TIVRDLVSAC AKQCPKALIG VVSNPVNSVV PIASEVLKKA GVFDPARLFG ITTLDVVRAR TFVAEAAGKS PYDVNVQVVG GHSGPTIIPL LSQAGVSLTE EQVKAITRRV QYGGDEVVKA KGGAGSATLS MAYAAGEWMS SVLKGLRGDK GIVECTYVQT DIIPGVNFFG CPVELGKGGV EKIHKPVFDA YEQSLLEKCV ADLEKNIAAG FAFGSRQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtc aaccgttatc gcttatcctg aaaaccaatc ctcttgtgtc tcacttgagt tgttatgaca tacatggtgt gacaggcgtg gctgcagacc tctcgcacat ttgttctcca gctaaggtga ctggtcacct gaaagatgaa cttcacaaag cagttgacgg agctgacgtt gtcatcatcc ccgcgggcac accgcgaaag ccggggatga ctcgcgaaga tctcttcagt gttaatgcaa cgatcgttcg agacctcgta tcggcttgtg caaaacaatg ccccaaggct cttatcggtg ttgtttccaa ccctgtcaac agtgttgtac ctattgcttc ggaggtgctc aaaaaggctg gtgtctttga ccctgctcgc ctctttggca taaccacgct tgatgtggtg agagctcgaa catttgtggc tgaggccgct ggtaagagtc cgtatgacgt taatgttcag gtggtgggtg gacacagtgg tccgactatt attccattat tatctcaagc tggtgtatca ttgacggagg aacaagtcaa ggctataacg cgacgagtgc agtatggcgg tgacgaagtt gtgaaagcta aaggaggggc gggttcagca actctttcaa tggcgtacgc cgctggggaa tggatgtcct ccgtgttgaa agggcttcgt ggtgacaagg gtatcgtgga gtgtacgtat gttcagacgg atattattcc cggcgtgaac tttttcggtt gcccggtgga acttgggaaa ggtggcgttg aaaagattca caaacctgtg tttgacgcgt atgaacagag tcttttggag aaatgtgttg cggacttgga gaaaaatatc gcagcaggtt ttgccttcgg ttccagacag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat