SSGCID: Target Page

TogoA.19925.a

Uncharacterized protein

CENTER ID:	TogoA.19925.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.19925.a.B5.GE42269	soluble domain #4		1679	2194
TogoA.19925.a.B6.GE42270	soluble domain #5		2217	2368

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.19925.a.B6.PW38471	soluble domain #5		2217	2368
TogoA.19925.a.B6.PW38796	soluble domain #5		2217	2368

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_272370
UniProt:	S8F1T1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTMDHFDSHY SKDYEMGRKH IQTQEPFSPF DAHQSGPCRS TVRPSFALDR SHGCKREFKG LKQYLPKCGR LVSDPSRLVS SGLMSHWWRP ECRVSNEGSP LCTSWCRLVA TSTKESADSV NAGHRSLFPE CILSCRQKPV TDGLREQLFA SDCRVSCLSN RTVHLPQGPY KTCSFRAFRR TLQCMLIGLL VKLSLVPVPS GGQMTLSSAD SSVNVAGSAI DEGHFSDQTQ GPAGEISLVG DAFSESLQIQ ADEAQASSDD SNGAPLRQEF PVKSSQTLPP GMNVQKPRKL KCSPVQLSLN LWAASSTVDV GNVRMKAVGY RGRDGRNEKP SAIEKGRSED VETVNGCALP GSWLTRAHFE LLPLSTRLNN GYYVRWSSPS VELLDGGKGD RATGANLAGN AGRPRELQQV EGDEHELPEE ERPSDNLLFT TQFVCTAAKV QSTSGKPTVL LNLSKSLASP GSTLREALPS AIFRNRVREL PTIRRAGFAL LDWFVVPPRG ISSFSRAAEG YRQRKGGSST LGIRVLSRQS LPALKNPPGS TEGPPFSSGA QSAEALSAAV SWSEETGQGS TEFDFSAAPA RPSVPLSSAD GWDRGIVPAI KIYSGAISAY LVPLFIGFVL LIAVPFIVVG AFGICCRPDH CDGSVCCLGC LGKRCFWLWC HRYQPTSSGG DGASSHSPLE SNTTGCLAPV GSTSVHRSAV RPLQPLLPLS REGTTGLRAD SSGDDVVVKQ GGLPTFWAPR PAARVTCLIL LFLTLLGVVS CLLFVFSFSV QLRQAVDDAG CAATEFFVQL VHGDSAVGPD LPGPPETFPS TSASAAPRSV AEGPSPVKIV PSTYHLTATK ALPNRPLTLF PALPASSLYK VRGSPHFRPR DTATPLLDTR SEREEAQWGG IRPPFLSDKL LEDATILLSE HLGEEPGALL LARHGARLFR LSELAARTWE RGAIGESLVG LSALETSEVG KEITKEQLGA RSYDGGSNAE GMRRRRLSLF SSPYFSPYSS GGSLASLPEV VGREATSRDM QRFPDGSIAG GVNKTGSEEA MIKPLPLLSP ISRRLSADSM SPQSRDSEKG KIKKGSSPKQ PWYVKAVNTI LQWGRFFGFG RKKRGKKGDR KKAGGPRAPR LNSEGTGSEV YTGPRQQVTA DPAVQSTSFD NGPKESSGHP EPPGEFLTES PAFLSGDRKQ ENRDPQSQLK GGQLSVQPRE TLSEATRSDL SEGAPAEARA SPSTGNPEGV MNPSFFTSAL TNAEVRDPEH VFPLEWSEGG MSQPVPEQTS SEHENTDTSE DDQGALSTYD FSEESSAVGI AKRESEGNAV PGAHLPSQAD QPLRPQLATF PNEVTHASAE FVGARSTETS AGSLLGREGN VEASGIRDPA SVLVGVVAAV RELAANLPEE SQTRRALETG ENGPGVLPPL HDQLEGGAHL RQYAWGTSRS EGGVHQEARG SQKGGDEPRK EGGFAGLKPA LKLLKHIRDL SDGESPRGLQ KVLMNATATG LDANTDIAIL EEHLQNARIM VEDPSNQTPG PAGERFHRSL AFAIASPTFT VILSRIPRFH QHVRELKEQC QEMMVAVKDL SSELHSSLNG DLFHQVDDAV EDSSDRVQRG IRFLDTAKPH ATVALYVLTG LIGVLLVLML VYLGCLCFWW QPGETKCSTV PSLVFWPIAL FLAVFSFIIG GALLVVSVAQ VDACLFLSRE VQHPGVVEAL ALSWASHPGD VDAQKATEAA KACFATNTDK EPVERNLTKA LGLQDFSSSV GEFEQSLRRA FDSVNLHMMD QFLLGISFAH AVLQDTAWVF FFDTYALSSQ GASSRTNTYL PVLYSGLQDK ARTFSSPFDI PTAKLVGPPG VKREDLLTLP PMSGSSENVL EYLRVELQRI QAKRNRAGET VFTEANGEKD TATEASQTRL PPVPGTFGED NASGTFLLYG LLDIEEYIEP FYFETLHRDE PDRLARYRID EFFSVDAPQI QAEIARLKPD PEERIKYENA LWLAAVKQRL RTRGNPNGSQ PGRDRDAENT EATEDTRATA GSASTAMFTG NPLSTPLHLP FRCSDKIQLS DEELELVRAQ ERDSTRRLRD SIDGVDKRVW SEESQEAEGD TKILLPYARQ WRRCDYDQWM ELVESQEAAD LLVQTQQIYD RVHSSKKRAE ATVLAPAVDT LKRTQSLLSK MDCTPVFQRF QTVQRRMCHD TAESVGLIGI GYQFLGLAAL IIFIVLFCAW QTLVANRRLQ KRWESRDTNK SLPLCNWTVS PETRADSRDS GNGREAPWRC PTPDSGQSDP DSASRNTAES VDPTVSPLNT NSCWRNVRRT GTRNSRVDEH GIEVETPEPT MQQAKACARR AHRGSTLLPP IPSEQQASVD ETMWAHFENE ERPEWLVR

NT Sequence

atgacaatgg accattttga cagtcattac tcaaaggatt acgagatggg cagaaagcac atacaaacac aggagccctt ttcgccgttt gatgcacatc agagtgggcc atgccgatct acagtgcgac cctcatttgc tttggatcgg tcacatggct gtaaacgtga attcaagggt ctgaagcaat atctaccaaa gtgtggtaga cttgtttctg atccgtcgcg tctcgtgtcg tccggattga tgtcacactg gtggcgtcca gaatgtcggg tatcaaatga aggaagtccg ctgtgcactt cgtggtgtcg attagtagca acgtcaacta aggaatccgc tgattcggta aacgctggac acagaagtct gtttccagag tgtatcctct cttgtaggca gaagccggtg accgacggcc tcagagagca attgtttgcg agcgactgca gagtatcctg tctgtcgaat cgtaccgtac acctgccgca gggaccatac aaaacgtgtt cttttcgagc atttagacgg actttacaat gtatgttgat aggtttgctt gtcaagctgt cgttggtacc cgtcccgtcc ggtgggcaaa tgacgttgtc atcagcggat tcttctgtta acgtggcggg gtccgcaata gacgaaggac atttctcaga ccagacacag ggtccagcgg gtgaaatatc tttggttggt gacgcgtttt ccgagagtct ccagatacaa gcggacgagg ctcaggcaag tagcgatgac agcaacggag ccccgttacg acaggagttt cctgtcaaaa gtagtcagac acttccaccc gggatgaacg ttcagaagcc gcggaagctc aagtgctctc cagttcaatt aagcctcaac ctttgggcag ccagtagtac tgtcgatgtg gggaatgtga ggatgaaggc ggtggggtac cggggccgcg acggaaggaa tgagaagccg tcagcaattg agaaaggtag atcggaggac gtggaaaccg ttaacgggtg tgcgcttccc ggaagctggt tgacccgcgc ccattttgaa ttgctcccgc tatccactcg actcaacaac ggctattatg tgcgttggtc cagcccgtca gttgagttgt tggacggcgg caagggagac cgtgcaacgg gtgccaattt ggcgggaaat gctggacgac cgagggagct acagcaggta gagggggatg agcacgaact tccagaggaa gagcgtccct cggataacct cttgttcacc acacaattcg tatgtaccgc tgcgaaggtg cagtcgacat cgggaaagcc tacagttttg ctgaatctat ccaagtccct cgcttctccg ggctcaactc ttcgagaggc tctgccctca gctatttttc ggaaccgagt gcgagagttg cctacaattc ggcgagcagg cttcgcactg ttagactggt tcgtcgtgcc gccccggggt atctcgtcct tttcccgtgc tgcagagggc tatagacaaa ggaaaggcgg tagcagcacc cttggaatac gagtgctttc tcgacaatca ttgcctgcgc taaagaaccc ccctgggtct actgaaggtc ctcctttttc ttcaggagcc caatcggcgg aagctctgtc agccgcggtg tcttggtcgg aggagactgg acaaggtagc actgaattcg acttctccgc cgcaccggcg cggccttccg ttcctctttc gtctgcagat ggatgggaca ggggtatcgt gcctgccatt aagatttact caggtgccat atccgcgtac ttagtgccac tgtttatcgg gtttgtcctg ttgattgctg tcccattcat tgtcgtcggt gcgtttggga tttgttgtcg tcccgaccac tgcgatggca gtgtgtgctg tcttggctgt cttggcaagc gctgcttctg gctttggtgt catcgctacc agccgacgtc ttctggtggt gatggcgcgt cttcgcacag ccccctggag agcaacacca ccggatgttt ggcccctgtc ggatctacct ccgtacaccg aagtgcggtt cgtcctttac agccactact tcctctcagc agagagggga cgactggcct tcgcgcagat tccagtgggg acgatgtggt agtcaagcaa ggcggcctgc ctaccttttg ggctccaaga ccagctgcaa gggttacttg cttgattctt ctttttctga cacttctcgg ggttgtcagt tgtcttttat tcgttttcag cttttccgtt cagctccgac aggctgtcga cgacgcaggc tgtgctgcca ctgagttttt tgttcaactc gttcacggtg attccgcggt cggaccggac ctccctggcc cacctgaaac tttcccatcg acttcggcat ctgcggcacc ccggtccgta gcggaaggac cgtcgcctgt gaaaattgtg ccttctacat atcatctgac tgcaacgaaa gcactcccca accgccctct tactcttttc ccagcattgc cagcttccag cctatacaaa gtgcgcggca gtcctcactt tcgccctagg gatacggcga cgccactgtt ggacactagg tccgagagag aagaagcaca gtggggtggg attcggcctc ccttcctctc tgacaaattg ttggaagatg ccacgatcct tttgtcagaa cacctcggcg aggagcccgg tgcgcttctc ctagcacgcc atggcgcgag actgtttcgg ctcagcgaac tcgcagcgag aacatgggaa cgaggagcga taggcgaatc gttagtggga ctgtctgcgc tcgaaactag tgaagttggc aaggagataa cgaaagagca gcttggtgcc aggagttacg acggtggtag caatgccgaa ggcatgcgca ggaggcgtct ctcgttgttc tcctctccgt acttctctcc gtactcttca ggtggttctt tggcatcgtt gccagaggtg gtagggcggg aagcaacctc tagggatatg cagaggtttc cggacggcag catcgctggt ggtgtgaaca aaacgggctc tgaagaagct atgataaagc cgcttccctt actttcacca atctctcgca ggctaagcgc ggattcgatg tcaccacaaa gcagggatag cgagaagggg aagataaaga agggttcgtc tccaaaacag ccgtggtacg tcaaggctgt caatacgata ttgcagtggg gacggttttt cggcttcggt agaaagaaac gtggaaaaaa gggagaccgc aagaaggccg gaggccccag agcaccgcgg ttgaacagtg aaggcacggg cagtgaggta tatacaggac ctcgtcagca agtgacagca gaccctgctg tgcaaagcac aagtttcgat aacgggccaa aagagtcgtc tggacatccg gaaccgcctg gggagttttt gaccgaatct cccgcgtttc tgtcaggaga tcggaagcaa gaaaatcggg atcctcagtc tcaattgaag ggcggacagc tctctgtgca accacgcgaa actctttcag aggcaacccg ctctgatctc tctgaggggg caccagcaga ggcacgcgcg tcgccaagca ccggcaaccc agaaggcgta atgaatcctt cattcttcac ttctgctttg accaatgccg aagttcgtga tccagagcat gtcttcccgt tggagtggtc cgagggcggc atgagtcagc cggttccaga gcagacatcg tcagaacatg aaaacacaga tacatccgaa gacgatcagg gtgccttgtc aacttacgac ttttcagaag aatcaagcgc ggtgggaata gcgaagagag agtctgaagg caatgctgta cctggcgcac atctcccttc acaggctgat cagccgctga gaccgcagct tgccacattt ccgaacgagg tgacgcatgc gtcggcagag tttgtcggtg cgcggagtac ggagacatca gccggaagcc ttctggggcg cgaaggaaat gtcgaagctt caggcattcg agacccagcc tcagtgctgg tgggcgtggt ggctgcagtc cgtgaactgg cggcgaactt gccagaggag agccagacac gcagagctct cgaaacgggt gagaatggcc cgggtgttct gccgcctttg cacgaccagc tggaaggcgg tgctcatctt cgtcagtatg catgggggac ttcaaggagt gaaggcggtg tacatcaaga agccagagga agccaaaagg gcggagacga gccgagaaaa gaaggcggtt ttgcaggatt gaaacccgct ttgaagctgc tgaaacatat tcgggatctc tccgacggag agtcaccacg aggcttgcaa aaggtgctga tgaatgcgac ggcaacgggt ctggacgcca acaccgacat tgcgattctt gaggagcatc tccagaatgc acgcattatg gtagaggatc cttcgaacca aaccccgggc cctgctggcg aacggtttca ccgtagtttg gcgtttgcaa ttgcgtcgcc gacatttact gtgatcctct caagaattcc acgttttcat cagcatgtcc gagagctcaa ggaacaatgt caggaaatga tggtcgcagt caaagatctt tcaagtgaac tccacagttc cctgaacggt gacttgttcc accaagtcga tgacgccgtt gaggacagct ctgacagggt tcaaaggggc atccggttcc tggacacggc caagccgcat gcaacagtgg ccttgtatgt tctgacgggt ttgatcggcg ttttactcgt gctcatgctc gtctaccttg gctgcctgtg tttctggtgg caaccgggag aaacgaagtg ctccacagtc ccctctctcg tcttctggcc cattgcgctt ttcctcgccg tattcagttt catcatcgga ggtgcacttc tggttgtctc cgttgctcaa gtggacgctt gccttttcct ttcaagggaa gtgcaacacc ctggtgtcgt agaagccctt gccctttctt gggcatcaca ccctggagac gttgacgcgc aaaaagcgac tgaggccgca aaggcctgct ttgccaccaa cacagacaaa gagcctgtgg agagaaacct cactaaggct cttggcttgc aggacttctc aagttcagtt ggagagttcg agcagtctct gcgacgggcg ttcgactctg tcaacctgca tatgatggat cagtttcttc tcggcatttc ttttgcgcat gcggttctcc aagatacagc ttgggtgttc tttttcgaca cgtatgctct ctcttcacag ggagcttcga gtcgaaccaa cacctacctt ccggtgttgt acagcggctt acaagacaaa gcccgaacat tcagcagtcc cttcgatatt cctacagcca aactcgtggg cccgccaggc gtgaagcgtg aggacctgtt gacgttgcct cctatgagcg ggtcatcgga gaatgtcctt gaatacctaa gagtcgaatt acagcgaatt caggccaagc gtaatcgtgc aggcgagact gtgttcacgg aagccaacgg agagaaagat acggcaacgg aagcaagtca aacgcgtttg ccgccggtac cgggtacatt cggcgaggac aacgcaagcg gaacatttct tttgtacggc ttgttggaca ttgaagagta catagagccg ttttactttg agactcttca tcgggatgag ccagaccgct tggcgcgtta caggattgac gagtttttct cagtagatgc tcctcagatc caggcggaaa ttgcgcgact gaagccggat ccggaagaac gaatcaaata cgagaacgct ctctggttag ctgctgtcaa gcagcgccta agaaccaggg gcaacccgaa tggttctcag ccgggtagag atcgtgatgc tgagaacacg gaggcgactg aagacacacg tgccacagca ggctccgcat ccactgctat gttcactgga aatccgctct ctacacctct ccatttacca ttccggtgct cagacaagat ccagttgagt gatgaagaac tggaactcgt gcgcgcccaa gaacgcgaca gtaccaggcg tctacgtgat agcatcgatg gtgtcgataa gcgggtatgg tcagaggagt ctcaggaggc tgaaggtgat actaaaattc ttctccctta tgcgcggcag tggcgccggt gtgactatga ccagtggatg gaacttgtcg agagccagga agcagctgac cttctcgtac agactcaaca gatctatgac cgcgttcata gttcaaagaa acgtgccgaa gctacagttc tagctcctgc agtggacacc ctcaagcgaa cccaaagcct gctgagcaag atggactgca cgccagtatt tcagagattc cagacggtgc agcggcgcat gtgtcacgac actgcagaga gcgtaggttt aataggcatc ggttatcagt tcctgggcct cgcagctcta atcattttca ttgttctctt ctgtgcctgg caaaccctgg ttgcaaatcg gcgactgcag aagcgctggg aatcgcgtga tacaaacaag tcgttgccat tatgcaactg gacagtttca ccagaaaccc gagctgattc acgagacagt ggtaacggac gtgaggcgcc atggcggtgt ccaacgccag atagcgggca aagtgaccct gacagcgcta gccgaaatac tgcggaatca gtggacccga cggtttctcc ccttaataca aattcatgct ggaggaatgt ccgccggacc ggaacgagaa acagcagagt tgacgaacac gggattgaag ttgaaacgcc agaacccact atgcaacaag ccaaagcttg tgcacgccgt gcccacagag ggagcaccct cctgccgccg ataccaagcg agcaacaagc atccgtggat gaaaccatgt gggcacactt tgagaacgaa gaacgtcctg agtggctagt caggtga

Details for TogoA.19925.a.B5.GE42269

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	59kDa
OBSERVED MW:	59kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMA QVDACLFLSR EVQHPGVVEA LALSWASHPG DVDAQKATEA AKACFATNTD KEPVERNLTK ALGLQDFSSS VGEFEQSLRR AFDSVNLHMM DQFLLGISFA HAVLQDTAWV FFFDTYALSS QGASSRTNTY LPVLYSGLQD KARTFSSPFD IPTAKLVGPP GVKREDLLTL PPMSGSSENV LEYLRVELQR IQAKRNRAGE TVFTEANGEK DTATEASQTR LPPVPGTFGE DNASGTFLLX GLLDIEEXXX PXXFXXLHXD XXDRLARYRI DEFFSVDAPQ IQAEIARLKP DPEERIKYEN ALWLAAVKQR LRTRGNPNGS QPGRDRDAEN TEATEDTRAT AGSASTAMFT GNPLSTPLHL PFRCSDKIQL SDEELELVRA QERDSTRRLR DSIDGVDKRV WSEESQEAEG DTKILLPYAR QWRRCDYDQW MELVESQEAA DLLVQTQQIY DRVHSSKKRA EATVLAPAVD TLKRTQSLLS KMDCTPVFQR FQTVQRRMCH DTAESVG

Validated NT Sequence

taaacctacg ctctctgcag tgtcgtgaca catgcgccgc tgcaccgtct ggaatctctg aaatactggc gtgcagtcca tcttgctcag caggctttgg gttcgcttga gggtgtccac tgcaggagct agaactgtag cttcggcacg tttctttgaa ctatgaacgc ggtcatagat ctgttgagtc tgtacgagaa ggtcagctgc ttcctggctc tcgacaagtt ccatccactg gtcatagtca caccggcgcc actgccgcgc ataagggaga agaattttag tatcaccttc agcctcctga gactcctctg accatacccg cttatcgaca ccatcgatgc tatcacgtag acgcctggta ctgtcgcgtt cttgggcgcg cacgagttcc agttcttcat cactcaactg gatcttgtct gagcaccgga atggtaaatg gagaggtgta gagagcggat ttccagtgaa catagcagtg gatgcggagc ctgctgtggc acgtgtgtct tcagtcgcct ccgtgttctc agcatcacga tctctacccg gctgagaacc attcgggttg cccctggttc ttaggcgctg cttgacagca gctaaccaga gagcgttctc gtatttgatt cgttcttccg gatccggctt cagtcgcgca atttccgcct ggatctgagg agcatctact gagaaaaact cgtcaatcct gtaacgcgcc aagcggtcnn nntnatccng atgaagnnnn ncaaagnnan ncggcnnnan gtnctcttca atgtccaaca agccnnncaa aagaaatgtt ccgcttgcgt tgtcctcgcc gaatgtaccc ggtaccggcg gcaaacgcgt ttgacttgct tccgttgccg tatctttctc tccgttggct tccgtgaaca cagtctcgcc tgcacgatta cgcttggcct gaattcgctg taattcgact cttaggtatt caaggacatt ctccgatgac ccgctcatag gaggcaacgt caacaggtcc tcacgcttca cgcctggcgg gcccacgagt ttggctgtag gaatatcgaa gggactgctg aatgttcggg ctttgtcttg taagccgctg tacaacaccg gaaggtaggt gttggttcga ctcgaagctc cctgtgaaga gagagcatac gtgtcgaaaa agaacaccca agctgtatct tggagaaccg catgcgcaaa agaaatgccg agaagaaact gatccatcat atgcaggttg acagagtcga acgcccgtcg cagagactgc tcgaactctc caactgaact tgagaagtcc tgcaagccaa gagccttagt gaggtttctc tccacaggct ctttgtctgt gttggtggca aagcaggcct ttgcggcctc agtcgctttt tgcgcgtcaa cgtctccagg gtgtgatgcc caagaaaggg caagggcttc tacgacacca gggtgttgca cttcccttga aaggaaaagg caagcgtcca cttgagccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHAQ VDACLFLSRE VQHPGVVEAL ALSWASHPGD VDAQKATEAA KACFATNTDK EPVERNLTKA LGLQDFSSSV GEFEQSLRRA FDSVNLHMMD QFLLGISFAH AVLQDTAWVF FFDTYALSSQ GASSRTNTYL PVLYSGLQDK ARTFSSPFDI PTAKLVGPPG VKREDLLTLP PMSGSSENVL EYLRVELQRI QAKRNRAGET VFTEANGEKD TATEASQTRL PPVPGTFGED NASGTFLLYG LLDIEEYIEP FYFETLHRDE PDRLARYRID EFFSVDAPQI QAEIARLKPD PEERIKYENA LWLAAVKQRL RTRGNPNGSQ PGRDRDAENT EATEDTRATA GSASTAMFTG NPLSTPLHLP FRCSDKIQLS DEELELVRAQ ERDSTRRLRD SIDGVDKRVW SEESQEAEGD TKILLPYARQ WRRCDYDQWM ELVESQEAAD LLVQTQQIYD RVHSSKKRAE ATVLAPAVDT LKRTQSLLSK MDCTPVFQRF QTVQRRMCHD TAESVGLIGI GYQF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctcaag tggacgcttg ccttttcctt tcaagggaag tgcaacaccc tggtgtcgta gaagcccttg ccctttcttg ggcatcacac cctggagacg ttgacgcgca aaaagcgact gaggccgcaa aggcctgctt tgccaccaac acagacaaag agcctgtgga gagaaacctc actaaggctc ttggcttgca ggacttctca agttcagttg gagagttcga gcagtctctg cgacgggcgt tcgactctgt caacctgcat atgatggatc agtttcttct cggcatttct tttgcgcatg cggttctcca agatacagct tgggtgttct ttttcgacac gtatgctctc tcttcacagg gagcttcgag tcgaaccaac acctaccttc cggtgttgta cagcggctta caagacaaag cccgaacatt cagcagtccc ttcgatattc ctacagccaa actcgtgggc ccgccaggcg tgaagcgtga ggacctgttg acgttgcctc ctatgagcgg gtcatcggag aatgtccttg aatacctaag agtcgaatta cagcgaattc aggccaagcg taatcgtgca ggcgagactg tgttcacgga agccaacgga gagaaagata cggcaacgga agcaagtcaa acgcgtttgc cgccggtacc gggtacattc ggcgaggaca acgcaagcgg aacatttctt ttgtacggct tgttggacat tgaagagtac atagagccgt tttactttga gactcttcat cgggatgagc cagaccgctt ggcgcgttac aggattgacg agtttttctc agtagatgct cctcagatcc aggcggaaat tgcgcgactg aagccggatc cggaagaacg aatcaaatac gagaacgctc tctggttagc tgctgtcaag cagcgcctaa gaaccagggg caacccgaat ggttctcagc cgggtagaga tcgtgatgct gagaacacgg aggcgactga agacacacgt gccacagcag gctccgcatc cactgctatg ttcactggaa atccgctctc tacacctctc catttaccat tccggtgctc agacaagatc cagttgagtg atgaagaact ggaactcgtg cgcgcccaag aacgcgacag taccaggcgt ctacgtgata gcatcgatgg tgtcgataag cgggtatggt cagaggagtc tcaggaggct gaaggtgata ctaaaattct tctcccttat gcgcggcagt ggcgccggtg tgactatgac cagtggatgg aacttgtcga gagccaggaa gcagctgacc ttctcgtaca gactcaacag atctatgacc gcgttcatag ttcaaagaaa cgtgccgaag ctacagttct agctcctgca gtggacaccc tcaagcgaac ccaaagcctg ctgagcaaga tggactgcac gccagtattt cagagattcc agacggtgca gcggcgcatg tgtcacgaca ctgcagagag cgtaggttta ataggcatcg gttatcagtt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for TogoA.19925.a.B6.GE42270

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	18kDa
OBSERVED MW:	18kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR RLQKRWESRD TNKSLPLCNW TVSPETRADS RDSGNGREAP WRCPTPDSGQ SDPDSASRNT AESVDPTVSP LNTNSCWRNV RRTGTRNSRV DEHGIEVETP EPTMQQAKAC ARRAHRGSTL LPPIPSEQQA SVDETMWAHF ENEERPEWLV R

Validated NT Sequence

atcttactca cttacctgac tagccactca ggacgttctt cgttctcaaa gtgtgcccac atggtttcat ccacggatgc ttgttgctcg cttggtatcg gcggcaggag ggtgctccct ctgtgggcac ggcgtgcaca agctttggct tgttgcatag tgggttctgg cgtttcaact tcaatcccgt gttcgtcaac tctgctgttt ctcgttccgg tccggcggac attcctccag catgaatttg tattaagggg agaaaccgtc gggtccactg attccgcagt atttcggcta gcgctgtcag ggtcactttg cccgctatct ggcgttggac accgccatgg cgcctcacgt ccgttaccac tgtctcgtga atcagctcgg gtttctggtg aaactgtcca gttgcataat ggcaacgact tgtttgtatc acgcgattcc cagcgcttct gcagtcgccg catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHRR LQKRWESRDT NKSLPLCNWT VSPETRADSR DSGNGREAPW RCPTPDSGQS DPDSASRNTA ESVDPTVSPL NTNSCWRNVR RTGTRNSRVD EHGIEVETPE PTMQQAKACA RRAHRGSTLL PPIPSEQQAS VDETMWAHFE NEERPEWLVR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcggcgac tgcagaagcg ctgggaatcg cgtgatacaa acaagtcgtt gccattatgc aactggacag tttcaccaga aacccgagct gattcacgag acagtggtaa cggacgtgag gcgccatggc ggtgtccaac gccagatagc gggcaaagtg accctgacag cgctagccga aatactgcgg aatcagtgga cccgacggtt tctcccctta atacaaattc atgctggagg aatgtccgcc ggaccggaac gagaaacagc agagttgacg aacacgggat tgaagttgaa acgccagaac ccactatgca acaagccaaa gcttgtgcac gccgtgccca cagagggagc accctcctgc cgccgatacc aagcgagcaa caagcatccg tggatgaaac catgtgggca cactttgaga acgaagaacg tcctgagtgg ctagtcaggt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for TogoA.19925.a.B6.PW38471

PURIFICATION DATe:	5/14/2018
CONCENTRATION:	5.21mg/ml
OBSERVED MW:	18kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMR RLQKRWESRD TNKSLPLCNW TVSPETRADS RDSGNGREAP WRCPTPDSGQ SDPDSASRNT AESVDPTVSP LNTNSCWRNV RRTGTRNSRV DEHGIEVETP EPTMQQAKAC ARRAHRGSTL LPPIPSEQQA SVDETMWAHF ENEERPEWLV R

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHRR LQKRWESRDT NKSLPLCNWT VSPETRADSR DSGNGREAPW RCPTPDSGQS DPDSASRNTA ESVDPTVSPL NTNSCWRNVR RTGTRNSRVD EHGIEVETPE PTMQQAKACA RRAHRGSTLL PPIPSEQQAS VDETMWAHFE NEERPEWLVR

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCGGCGAC TGCAGAAGCG CTGGGAATCG CGTGATACAA ACAAGTCGTT GCCATTATGC AACTGGACAG TTTCACCAGA AACCCGAGCT GATTCACGAG ACAGTGGTAA CGGACGTGAG GCGCCATGGC GGTGTCCAAC GCCAGATAGC GGGCAAAGTG ACCCTGACAG CGCTAGCCGA AATACTGCGG AATCAGTGGA CCCGACGGTT TCTCCCCTTA ATACAAATTC ATGCTGGAGG AATGTCCGCC GGACCGGAAC GAGAAACAGC AGAGTTGACG AACACGGGAT TGAAGTTGAA ACGCCAGAAC CCACTATGCA ACAAGCCAAA GCTTGTGCAC GCCGTGCCCA CAGAGGGAGC ACCCTCCTGC CGCCGATACC AAGCGAGCAA CAAGCATCCG TGGATGAAAC CATGTGGGCA CACTTTGAGA ACGAAGAACG TCCTGAGTGG CTAGTCAGGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT

Details for TogoA.19925.a.B6.PW38796

PURIFICATION DATe:	1/31/2020
CONCENTRATION:	7mg/ml
OBSERVED MW:	18kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMR RLQKRWESRD TNKSLPLCNW TVSPETRADS RDSGNGREAP WRCPTPDSGQ SDPDSASRNT AESVDPTVSP LNTNSCWRNV RRTGTRNSRV DEHGIEVETP EPTMQQAKAC ARRAHRGSTL LPPIPSEQQA SVDETMWAHF ENEERPEWLV R

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHRR LQKRWESRDT NKSLPLCNWT VSPETRADSR DSGNGREAPW RCPTPDSGQS DPDSASRNTA ESVDPTVSPL NTNSCWRNVR RTGTRNSRVD EHGIEVETPE PTMQQAKACA RRAHRGSTLL PPIPSEQQAS VDETMWAHFE NEERPEWLVR

Full NT Sequence (Expression Vector + Insert)