TogoA.19788.a

Uncharacterized protein

CENTER ID: TogoA.19788.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.19788.a.B2.GE41995 Sheiner lab construct 31 342
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGGT1_310770
UniProt: S7UU87

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMKQSFSRLA VRSVCTFLLC WTVLPVWTAF LTSPSPGPQQ HLLLADALAT PHLSISAFSI EPTQSLLLPR SSLAHFPPCE VPRASNPRLL CAIHLNVEQS EVHPRGSRVF FRDRRKEFLT HGGTLNQSLR ALVHGQDKRN SHCSLSPGAS TAVPSSTGLG RRGSLAFLWR QNTGKQEKLP DLLAFVGEEC EYCRRMEPVE KAVEDLLPGT KIHRLEVWHN ALNFQLLQEL DRDGKCGGLP FYFNIRTLQW ICGATTKTNL LAWASHQPCK SHEPPALQAE DLEVLNRRTG MFARLMRRVD KMRHEGEKAM QQTLQEQRRG REREQAEQRR LQGEADRSKR KD
NT Sequence
atgatgaagc aatccttttc ccggctggct gttcgctctg tctgcacgtt cctattgtgt tggacggttt taccagtgtg gactgcgttt ctcacaagtc cgtctcccgg tccgcagcaa cacctactgc ttgcggatgc tctagccacc ccgcacttgt caatttctgc gttctccatt gagcccacac aatcccttct gttgccgcga agctctctcg cacattttcc accgtgtgaa gttccccgcg catcgaaccc gagacttctc tgcgccatcc atttaaacgt agagcagtca gaagtccacc cccgcggatc aagggtcttc tttcgggatc gtaggaaaga gtttttaact cacggaggaa cactgaacca gagtctccga gcccttgttc acggacaaga caagagaaat tcgcattgtt cactctcccc tggagcgtcc acggccgtgc catcttcaac aggactagga cgacgaggat ccctagcttt cttgtggcga cagaacacag gcaaacaaga gaaacttcct gaccttcttg cttttgttgg ggaggaatgc gaatactgta gaagaatgga gcctgtcgag aaggcggtgg aggatctctt acctggcaca aaaatccaca gactcgaagt gtggcacaac gcgctgaact ttcagctttt gcaggaactg gatcgagatg gcaagtgcgg tgggcttccc ttctacttca acatacggac tctgcagtgg atttgtggag cgacgacgaa gacgaatctc ttggcatggg catcgcacca accgtgcaaa tcccatgagc cgccggctct tcaagcggaa gacttggagg ttctcaatcg ccgcacagga atgtttgccc gtctgatgag acgtgttgac aaaatgcgac atgaaggcga gaaggcaatg cagcagactc tccaggagca gcgccggggt cgagaacgtg aacaggccga acagcgaagg cttcaaggag aggcagatcg ctcgaaacga aaggactga
Details for TogoA.19788.a.B2.GE41995
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML TSPSPGPQQH LLLAGALATP HLSISAFSIE PTQSLLLPRS SLAHFPPCEV PRASNPRLLC AIHLNVEQSE VHPRGSRVFF RDRRKEFLTH GGTLNQSLRA LVHGQDKRNS HCSLSPGAST AVPSSTGLGR RGSLAFLWRQ NTGKQEKLPD LLAFVGEECE YCRRMEPVEK AVEDLLPGTK IHRLEVWHNA LNFQLLQELD RDGKCGGLPF YFNIRTLQWI CGATTKTNLL AWASHQPCKS HEPPALQAED LEVLNRRTGM FARLMRRVDK MRHEGEKAMQ QTLQEQRRGR EREQAEQRRL QGXXDRSKRK D
Validated NT Sequence
tcacttagtc ctttcgtttc gagcgatctn nntctccttg aagccttcgc tgttcggcct gttcacgttc tcgaccccgg cgctgctcct ggagagtctg ctgcattgcc ttctcgcctt catgtcgcat tttgtcaaca cgtctcatca gacgggcaaa cattcctgtg cggcgattga gaacctccaa gtcttccgct tgaagagccg gcggctcatg ggatttgcac ggttggtgcg atgcccatgc caagagattc gtcttcgtcg tcgctccaca aatccactgc agagtccgta tgttgaagta gaagggaagc ccaccgcact tgccatctcg atccagttcc tgcaaaagct gaaagttcag cgcgttgtgc cacacttcga gtctgtggat ttttgtgcca ggtaagagat cctccaccgc cttctcgaca ggctccattc ttctacagta ttcgcattcc tccccaacaa aagcaagaag gtcaggaagt ttctcttgtt tgcctgtgtt ctgtcgccac aagaaagcta gggatcctcg tcgtcctagt cctgttgaag atggcacggc cgtggacgct ccaggggaga gtgaacaatg cgaatttctc ttgtcttgtc cgtgaacaag ggctcggaga ctctggttca gtgttcctcc gtgagttaaa aactctttcc tacgatcccg aaagaagacc cttgatccgc gggggtggac ttctgactgc tctacgttta aatggatggc gcagagaagt ctcgggttcg atgcgcgggg aacttcacac ggtggaaaat gtgcgagaga gcttcgcggc aacagaaggg attgtgtggg ctcaatggag aacgcagaaa ttgacaagtg cggggtggct agagcacccg caagcagtag gtgttgctgc ggaccgggag acggacttgt gagcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHLT SPSPGPQQHL LLADALATPH LSISAFSIEP TQSLLLPRSS LAHFPPCEVP RASNPRLLCA IHLNVEQSEV HPRGSRVFFR DRRKEFLTHG GTLNQSLRAL VHGQDKRNSH CSLSPGASTA VPSSTGLGRR GSLAFLWRQN TGKQEKLPDL LAFVGEECEY CRRMEPVEKA VEDLLPGTKI HRLEVWHNAL NFQLLQELDR DGKCGGLPFY FNIRTLQWIC GATTKTNLLA WASHQPCKSH EPPALQAEDL EVLNRRTGMF ARLMRRVDKM RHEGEKAMQQ TLQEQRRGRE REQAEQRRLQ GEADRSKRKD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctcacaa gtccgtctcc cggtccgcag caacacctac tgcttgcgga tgctctagcc accccgcact tgtcaatttc tgcgttctcc attgagccca cacaatccct tctgttgccg cgaagctctc tcgcacattt tccaccgtgt gaagttcccc gcgcatcgaa cccgagactt ctctgcgcca tccatttaaa cgtagagcag tcagaagtcc acccccgcgg atcaagggtc ttctttcggg atcgtaggaa agagttttta actcacggag gaacactgaa ccagagtctc cgagcccttg ttcacggaca agacaagaga aattcgcatt gttcactctc ccctggagcg tccacggccg tgccatcttc aacaggacta ggacgacgag gatccctagc tttcttgtgg cgacagaaca caggcaaaca agagaaactt cctgaccttc ttgcttttgt tggggaggaa tgcgaatact gtagaagaat ggagcctgtc gagaaggcgg tggaggatct cttacctggc acaaaaatcc acagactcga agtgtggcac aacgcgctga actttcagct tttgcaggaa ctggatcgag atggcaagtg cggtgggctt cccttctact tcaacatacg gactctgcag tggatttgtg gagcgacgac gaagacgaat ctcttggcat gggcatcgca ccaaccgtgc aaatcccatg agccgccggc tcttcaagcg gaagacttgg aggttctcaa tcgccgcaca ggaatgtttg cccgtctgat gagacgtgtt gacaaaatgc gacatgaagg cgagaaggca atgcagcaga ctctccagga gcagcgccgg ggtcgagaac gtgaacaggc cgaacagcga aggcttcaag gagaggcaga tcgctcgaaa cgaaaggact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat