SSGCID: Target Page

TogoA.18611.a

Thioredoxin, putative; Nucleoredoxin, putative; EC5.3.4.1

CENTER ID:	TogoA.18611.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.18611.a.B2.GE37766	thioredoxin domains 1 and 2		306	742
TogoA.18611.a.B3.GE37767	N-terminal domain		55	260

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.18611.a.B2.PS02016	thioredoxin domains 1 and 2		306	742	unavailable

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_312110
RefSeq:	XP_002364433.1
UniProt:	B6K8Q9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAPDSCRPDP VGAPNGRGSL KEVSSSFCTT SSLFSRLSLL FLLFSLFASP RQLSLTSAIS LEPSPGSPAA AQVSSGVAPT PVEKESGNGG QVKVHLKRDE AGRPLPVSAA SLFAAEKPHE EEEREVLRVG VDAGDSRREK RAVEPTRMLA SIDPQHPSVS AQVVSPAPSS SLTAPPTARP RDEGNNTCVR NNETCQRRER YAEKSIQRHR HDSTSSRESV AFASFGSSKN SFATAYAPFD DEDEEGALAH LKNKVKTAVS CPAAKILLGI SGVVALGTLG IKYTRNVLRR RRREGLQRPP TPFTDLLGHV LLKRERGKHA AETRELATGD VLGKPGSVTA LYFSGRGVEE MLQTRGYQPF TPRLERIVEG CRERGQELNV VYLSADADSS DAEKHFSDMS WYALPFDDAP GQARIHRLFR KFRVSTLPHV VLLDSNARVL NSHAYASMIV RPTAFPWKKQ TPAELLGDAF VAGEGQKVGK DALDNNVVGI YFSASWCPPC QAFTPKLVEA LKGWKEQGKD VSVVFVSNDR DEKAFEEYFK KMEGFVAIPF ADTTRRALLQ EALNVRSLPT LVWLSKEGEI LTRRGVPHVL EDPEGRNFPW KDKDVNDVSD SVEGIADEPA LILFMEHLDE KAKEEQEKAL EEAMQALQSQ KNDGGVPPLP RLFTAKSLSP RSIALRRICR QDPPDADKEK KGPILTIVDL LDQSYFTALQ EPGRIFSADE IVAFINKFRR EELERNSLAV PE

NT Sequence

atggcgcctg acagttgcag accggacccc gtgggggctc ccaatggacg gggatccctg aaggaggttt cctcctcttt ctgcacaacg tcttctctct tttctcgcct ctctctcctc ttcctgctct tctctctctt cgcttcgcct cgacagctct ccctaacgtc ggccatctcg ctggagcctt cgccgggatc tcccgctgcg gcccaagtgt cgtcaggcgt cgcccccaca ccggtcgaga aagagtcggg caacggcggc caggtgaagg tccacctgaa acgcgacgag gcaggccggc cactgccggt gtctgcggcc tctttgtttg cagccgagaa gcctcacgaa gaggaggaaa gggaggtctt gcgcgtcgga gtggacgcag gagactctcg gagagagaaa cgcgcagtcg aacccacgcg catgcttgct tcgatcgatc cgcagcatcc ttcggtctct gcgcaagtcg tctccccggc gccttcctct tctctcacag cgcctccaac ggcgcgaccg cgcgacgaag gaaacaatac ttgcgtcaga aacaacgaga catgtcaacg gcgcgaaaga tatgcggaga aatccattca acggcatcga cacgattcca cctcctcgcg ggagagcgtc gcttttgcgt ccttcggatc ttcaaagaat tcgtttgcaa ctgcctacgc cccgttcgac gacgaagacg aagaaggcgc tcttgcacac ttgaaaaata aagtgaagac tgctgtctcc tgccctgcag caaaaattct gctcggcatc agtggagtcg tcgctctagg gacgcttggc atcaaataca cgagaaacgt tttgcggcgc cgtcgacgcg aaggcctaca gaggccaccg acacctttca cagatctgct cggccatgtt ctgctgaagc gcgaaagagg aaaacatgcg gccgagactc gcgagctcgc aacgggtgat gtgctcggca aacctggaag cgtgacggcg ctttactttt cagggcgagg cgttgaggaa atgttgcaga ctcgagggta ccagcctttt acgcctcgcc tagagcgcat tgttgaggga tgccgcgagc gaggacaaga actgaatgtc gtgtatctct ctgcagatgc cgactccagc gacgccgaaa aacatttcag cgacatgtcc tggtatgccc tgccgtttga cgacgctcct gggcaggcgc ggatccaccg acttttccgg aaattccgag tttcgacttt gcctcacgtt gtgcttctcg actccaatgc gcgtgttctt aattcgcatg cgtacgcgtc gatgattgtc cgtccgacgg cttttccatg gaaaaagcag acgccggcgg agcttctcgg cgacgccttc gtcgccggcg aaggccagaa ggtcggaaaa gacgctcttg acaataacgt cgtgggcatc tacttcagcg cctcttggtg tccgccttgc caggcgttta ctcccaaact cgtggaggct ctgaagggat ggaaagaaca agggaaggat gtctcagttg ttttcgtcag caacgatcgc gatgaaaaag cgttcgaaga gtacttcaag aaaatggaag gcttcgtcgc cattcccttc gccgacacca cccgccgagc cctactgcag gaggcgctga atgtgcggtc tttgcccacc ttggtgtggc tgtcgaagga aggggaaata ttgacaaggc gaggcgttcc gcatgtcctg gaagatcccg agggaagaaa ctttccgtgg aaagacaagg atgtgaacga cgtgagcgac tctgtcgaag gcattgcaga cgagcctgct ctcattctct tcatggagca cctggacgag aaggcgaagg aagaacaaga aaaggccctc gaggaagcga tgcaggcctt gcagagtcag aagaatgacg gaggggtgcc gccgctgcct cggctgttca ccgcgaaatc gctttctccc aggtcgattg ctctccggag aatttgccga caagatccgc cagacgcaga caaagagaaa aaaggcccta ttctgaccat tgtggacttg ctggaccaga gctacttcac cgctctgcaa gagcccggca ggatcttctc ggccgacgag atcgtcgctt tcatcaacaa gttccgcaga gaggaactgg aaagaaattc tttggcggtc cccgaataa

Details for TogoA.18611.a.B2.GE37766

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/4/2014
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	45

Validated AA Sequence

MEGFVAIPFA DTTRRALLQE ALNVRSLPTL VWLSKEGEVL TRRGVPHVLE DPEGRNFPWK DKDVNDVSDS VEGIADEPAL ILFMEHLDEK AKEEQEKALE EAMQALQSQK NDGGVPPLPR LFTAKSLSPR SIALRRICRQ DLPDADKEKK GPILTIVDLL DQSYFTALQE PGRIFSADEI VAFINKFRRE ELERNSLAVP E

Validated NT Sequence

gctnctttcg ggctttgtta gcagccggat cctatcttac tcacttattc ggggaccgcc aaagaatttc tttccagttc ctctctgcgg aacttgttga tgaaagcgac gatctcgtcg gccgagaaga tcctgccggg ctcttgcaga gcggtgaagt agctctggtc cagcaagtcc acaatggtca gaatagggcc ttttttctct ttgtctgcgt ctggcagatc ttgtcggcaa attctccgga gagcaatcga cctgggagaa agcgatttcg cggtgaacag ccgaggcagc ggcggcaccc ctccgtcatt cttctgactc tgcaacgcct gcatcgcttc ctcgagggcc ttttcttgtt cttccttcgc cttctcgtcc aggtgctcca tgaagagaat gagagcaggt tcgtctgcaa tgccttcgac agagtcgctc acgtcgttta cgtccttgtc tttccacgga aagtttcttc cctcgggatc ttccaggaca tgcggaacgc ctcgccttgt caatacttcc ccttccttcg acagccacac caaggtgggc aaagaccgca cattcagtgc ctcctgcagt agggctcggc gggtggtgtc ggcgaaggga atggcgacga agccttccat tttcttgaag tactcttcga acgctttttc atcgcgatcg ttgctgacga aaacaactga gacatccttc ccttgttctt tccatccctt cagagcctcc acgagtttgg gagtaaacgc ctggcaaggc ggacaccaag aggcgctgaa gtagatgccc acgacgttat tgtcaagagc gtcttttccg accttctggc cttcgccgga gacgaaggcg tcgccgagaa gctccgccgg cgtctgcttt ttccatggaa aagccgtcgn acggacatca tcgacgcgta cgcatgcgag taagacacgc gcattggagt cgagaagcac aacgtgaggc aaagtcgaaa ctcganttcc naaagtcgtg gatccgcgct gcccnnnnng tcgtcaangn anggnatacc ngncatgtng ctgaangttt tngcntcnct ggangnatct gcnannnnnn ncgacntnan ttcttgnncn tcgcntnngn nnccnnnatg cgctnnngnn nggnnnnnaa gntgtacnnn nnngnnnnnn cncncntgcc tnannannnn nnnnnnnnnn nnnnncannn nnnnnnnnc

Expected Protein Sequence

MAHHHHHHLL GHVLLKRERG KHAAETRELA TGDVLGKPGS VTALYFSGRG VEEMLQTRGY QPFTPRLERI VEGCRERGQE LNVVYLSADA DSSDAEKHFS DMSWYALPFD DAPGQARIHR LFRKFRVSTL PHVVLLDSNA RVLNSHAYAS MIVRPTAFPW KKQTPAELLG DAFVAGEGQK VGKDALDNNV VGIYFSASWC PPCQAFTPKL VEALKGWKEQ GKDVSVVFVS NDRDEKAFEE YFKKMEGFVA IPFADTTRRA LLQEALNVRS LPTLVWLSKE GEILTRRGVP HVLEDPEGRN FPWKDKDVND VSDSVEGIAD EPALILFMEH LDEKAKEEQE KALEEAMQAL QSQKNDGGVP PLPRLFTAKS LSPRSIALRR ICRQDPPDAD KEKKGPILTI VDLLDQSYFT ALQEPGRIFS ADEIVAFINK FRREELERNS LAVPE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctgctcg gccatgttct gctgaagcgc gaaagaggaa aacatgcggc cgagactcgc gagctcgcaa cgggtgatgt gctcggcaaa cctggaagcg tgacggcgct ttacttttca gggcgaggcg ttgaggaaat gttgcagact cgagggtacc agccttttac gcctcgccta gagcgcattg ttgagggatg ccgcgagcga ggacaagaac tgaatgtcgt gtatctctct gcagatgccg actccagcga cgccgaaaaa catttcagcg acatgtcctg gtatgccctg ccgtttgacg acgctcctgg gcaggcgcgg atccaccgac ttttccggaa attccgagtt tcgactttgc ctcacgttgt gcttctcgac tccaatgcgc gtgttcttaa ttcgcatgcg tacgcgtcga tgattgtccg tccgacggct tttccatgga aaaagcagac gccggcggag cttctcggcg acgccttcgt cgccggcgaa ggccagaagg tcggaaaaga cgctcttgac aataacgtcg tgggcatcta cttcagcgcc tcttggtgtc cgccttgcca ggcgtttact cccaaactcg tggaggctct gaagggatgg aaagaacaag ggaaggatgt ctcagttgtt ttcgtcagca acgatcgcga tgaaaaagcg ttcgaagagt acttcaagaa aatggaaggc ttcgtcgcca ttcccttcgc cgacaccacc cgccgagccc tactgcagga ggcgctgaat gtgcggtctt tgcccacctt ggtgtggctg tcgaaggaag gggaaatatt gacaaggcga ggcgttccgc atgtcctgga agatcccgag ggaagaaact ttccgtggaa agacaaggat gtgaacgacg tgagcgactc tgtcgaaggc attgcagacg agcctgctct cattctcttc atggagcacc tggacgagaa ggcgaaggaa gaacaagaaa aggccctcga ggaagcgatg caggccttgc agagtcagaa gaatgacgga ggggtgccgc cgctgcctcg gctgttcacc gcgaaatcgc tttctcccag gtcgattgct ctccggagaa tttgccgaca agatccgcca gacgcagaca aagagaaaaa aggccctatt ctgaccattg tggacttgct ggaccagagc tacttcaccg ctctgcaaga gcccggcagg atcttctcgg ccgacgagat cgtcgctttc atcaacaagt tccgcagaga ggaactggaa agaaattctt tggcggtccc cgaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for TogoA.18611.a.B3.GE37767

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/4/2014
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	30
PERCENT COVERAGE:	50

Validated AA Sequence

MAHHHHHHML TSAISPTLNR AERNLRSSSR GSQRCMAGQS SIHPHRSTGL QGISNFSTKS AGKGEETQQK RRQCMQRRKH QRAYPKNKKR EPQSFNKRQG SYPGKKAKR

Validated NT Sequence

atggctcacc accaccacca ccatatgcta acgtcggcca tctcgccgac tttgaaccgc gcagagcgaa atctgcgctc aagttcgcga ggctcccaac ggtgcatggc gggccagtcg tccatccatc cacacaggag cacgggttta caaggaattt cgaatttctc tacaaagtct gcaggaaaag gcgaggaaac ccagcaaaag agacgccaat gcatgcaaag aaggaagcac caaagagctt acccaaaaaa caaaaagcga gagcctcaga gttttaataa gagacagggg agctatccgg gcaagaaggc gaagaggtga aaggagagat gtatgattcg gaagaaaggc acagaaccaa gtacgcattc cacattcacc gacagacggt ctacgcttaa catttgagtc ggctgtcaac agaaatctca gtgccccctc catatgcata cgcatatata tatatatata tatatat

Expected Protein Sequence

MAHHHHHHLT SAISLEPSPG SPAAAQVSSG VAPTPVEKES GNGGQVKVHL KRDEAGRPLP VSAASLFAAE KPHEEEEREV LRVGVDAGDS RREKRAVEPT RMLASIDPQH PSVSAQVVSP APSSSLTAPP TARPRDEGNN TCVRNNETCQ RRERYAEKSI QRHRHDSTSS RESVAFASFG SSKNSFATAY APFDDEDEEG ALAHLKNKVK TAVS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctaacgt cggccatctc gctggagcct tcgccgggat ctcccgctgc ggcccaagtg tcgtcaggcg tcgcccccac accggtcgag aaagagtcgg gcaacggcgg ccaggtgaag gtccacctga aacgcgacga ggcaggccgg ccactgccgg tgtctgcggc ctctttgttt gcagccgaga agcctcacga agaggaggaa agggaggtct tgcgcgtcgg agtggacgca ggagactctc ggagagagaa acgcgcagtc gaacccacgc gcatgcttgc ttcgatcgat ccgcagcatc cttcggtctc tgcgcaagtc gtctccccgg cgccttcctc ttctctcaca gcgcctccaa cggcgcgacc gcgcgacgaa ggaaacaata cttgcgtcag aaacaacgag acatgtcaac ggcgcgaaag atatgcggag aaatccattc aacggcatcg acacgattcc acctcctcgc gggagagcgt cgcttttgcg tccttcggat cttcaaagaa ttcgtttgca actgcctacg ccccgttcga cgacgaagac gaagaaggcg ctcttgcaca cttgaaaaat aaagtgaaga ctgctgtctc ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for TogoA.18611.a.B2.PS02016

PURIFICATION DATe:	3/12/2014
CONCENTRATION:	39mg/ml
OBSERVED MW:	50kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	data unavailable
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	45

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTGCTCG GCCATGTTCT GCTGAAGCGC GAAAGAGGAA AACATGCGGC CGAGACTCGC GAGCTCGCAA CGGGTGATGT GCTCGGCAAA CCTGGAAGCG TGACGGCGCT TTACTTTTCA GGGCGAGGCG TTGAGGAAAT GTTGCAGACT CGAGGGTACC AGCCTTTTAC GCCTCGCCTA GAGCGCATTG TTGAGGGATG CCGCGAGCGA GGACAAGAAC TGAATGTCGT GTATCTCTCT GCAGATGCCG ACTCCAGCGA CGCCGAAAAA CATTTCAGCG ACATGTCCTG GTATGCCCTG CCGTTTGACG ACGCTCCTGG GCAGGCGCGG ATCCACCGAC TTTTCCGGAA ATTCCGAGTT TCGACTTTGC CTCACGTTGT GCTTCTCGAC TCCAATGCGC GTGTTCTTAA TTCGCATGCG TACGCGTCGA TGATTGTCCG TCCGACGGCT TTTCCATGGA AAAAGCAGAC GCCGGCGGAG CTTCTCGGCG ACGCCTTCGT CGCCGGCGAA GGCCAGAAGG TCGGAAAAGA CGCTCTTGAC AATAACGTCG TGGGCATCTA CTTCAGCGCC TCTTGGTGTC CGCCTTGCCA GGCGTTTACT CCCAAACTCG TGGAGGCTCT GAAGGGATGG AAAGAACAAG GGAAGGATGT CTCAGTTGTT TTCGTCAGCA ACGATCGCGA TGAAAAAGCG TTCGAAGAGT ACTTCAAGAA AATGGAAGGC TTCGTCGCCA TTCCCTTCGC CGACACCACC CGCCGAGCCC TACTGCAGGA GGCGCTGAAT GTGCGGTCTT TGCCCACCTT GGTGTGGCTG TCGAAGGAAG GGGAAATATT GACAAGGCGA GGCGTTCCGC ATGTCCTGGA AGATCCCGAG GGAAGAAACT TTCCGTGGAA AGACAAGGAT GTGAACGACG TGAGCGACTC TGTCGAAGGC ATTGCAGACG AGCCTGCTCT CATTCTCTTC ATGGAGCACC TGGACGAGAA GGCGAAGGAA GAACAAGAAA AGGCCCTCGA GGAAGCGATG CAGGCCTTGC AGAGTCAGAA GAATGACGGA GGGGTGCCGC CGCTGCCTCG GCTGTTCACC GCGAAATCGC TTTCTCCCAG GTCGATTGCT CTCCGGAGAA TTTGCCGACA AGATCCGCCA GACGCAGACA AAGAGAAAAA AGGCCCTATT CTGACCATTG TGGACTTGCT GGACCAGAGC TACTTCACCG CTCTGCAAGA GCCCGGCAGG ATCTTCTCGG CCGACGAGAT CGTCGCTTTC ATCAACAAGT TCCGCAGAGA GGAACTGGAA AGAAATTCTT TGGCGGTCCC CGAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT