TogoA.18505.a

NBP2B protein, putative

CENTER ID: TogoA.18505.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.18505.a.B1.GE35412 full length 1 318
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.18505.a.B1.PS01606 full length 1 318

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_318760
RefSeq: XP_002369814.1
UniProt: B9PVB2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKNGMEGGRF TALGHSRGLS SAASGHGSAS STAASVHGDH PSHFSSLASV QTSGPLSSLP SRSEGARALP TRTTSLSGKS DRLHRLEQRL SGLHAGLEKQ RQQRYQHLIE KLQHVEEDVA DVSAAGNEAQ SVFREELRKI EADLRERQSE LETDLARREQ QTQEMERRLT RMLQTEQEAL REVETSLMNA FTEKTKALRE EILQSGQLRE EQEASLRHYC EVEIPRLRDG LQQEVREREN MERRILKHAA EEVERLNGQL AAEREARERH LQSLLSLFEE VVREVQRDIA REKEDREKMQ EDLVHLMEQT IDRITTSI
NT Sequence
atgaagaacg gaatggaagg cggccgattc acggctctcg ggcactcgag gggtctctcc tcggcggctt ccggtcacgg ctctgccagc agcaccgccg ccagtgttca cggagaccat ccttcgcact tttctagtct cgcgtctgtg cagacgtcag gccccctttc gtctcttccc tctcgaagtg aaggcgcgag agctctcccg acgaggacga cgagcctcag cggcaaatcg gatagactcc accggctgga gcaaaggctc tcgggtctgc acgcaggact ggagaaacag aggcagcagc gctaccagca cctcattgaa aaactgcagc acgtggaaga agatgttgca gacgtcagcg ctgcaggcaa cgaagctcag tccgtcttcc gagaggagct gagaaaaatc gaagcagact tgcgcgagcg tcagagcgaa ctcgaaactg acttggcccg ccgagagcag cagactcagg agatggagag aagactgacg cgcatgctgc agacggagca ggaggcttta cgagaagttg aaacctcctt gatgaatgcg tttacagaga agacaaaggc tctaagagag gaaattcttc agagcggcca gctgcgggag gagcaggagg cgtcgctccg acactactgc gaagtcgaaa ttccccgcct ccgcgacggc cttcagcagg aagtgcggga aagggaaaac atggagagaa gaattctcaa gcatgcagcg gaggaggttg agcgactcaa tgggcagctt gccgcggaga gagaagctcg tgaacggcat ctgcagtctc tgctgtctct gttcgaagag gtcgtccgcg aggtgcaacg cgatatcgcc cgggaaaagg aggaccgaga gaaaatgcag gaagatctgg tccacctcat ggagcaaaca attgaccgca tcacaacatc tatatga
Details for TogoA.18505.a.B1.GE35412
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MKNGMEGGRF TALGHSRGLS SAASGHGSAS STAASVHGDH PSHFSSLASV QTSGPLSSLP SRSEGARALP TRTTSLSGKS DRLHRLEQRL SGLHAGLEKQ RQQRYQHLIE KLQHVEEDVA DVSAAGNEAQ SVFREELRKI EADLRERQSE LETDLARREQ QTQEMERRLT RMLQTEQEAL REVETSLMNA FTEKTKALRE EILQSGQLRE EQEASLRHYC EVEIPRLRDG LQQEVREREN MERRILKHAA EEVERLNGQL AAEREARERH LQSLLSLFEE VVREVQRDIA REKEDREKMQ EDLVHLME
Validated NT Sequence
ttgctccatg aggtggacca gatcttcctg cattttctct cggtcctcct tttcccgggc gatatcgcgt tgcacctcgc ggacgacctc ttcgaacaga gacagcagag actgcagatg ccgttcacga gcttctctct ccgcggcaag ctgcccattg agtcgctcaa cctcctccgc tgcatgcttg agaattcttc tctccatgtt ttccctttcc cgcacttcct gctgaaggcc gtcgcggagg cggggaattt cgacttcgca gtagtgtcgg agcgacgcct cctgctcctc ccgcagctgg ccgctctgaa gaatttcctc tcttagagcc tttgtcttct ctgtaaacgc attcatcaag gaggtttcaa cttctcgtaa agcctcctgc tccgtctgca gcatgcgcgt cagtcttctc tccatctcct gagtctgctg ctctcggcgg gccaagtcag tttcgagttc gctctgacgc tcgcgcaagt ctgcttcgat ttttctcagc tcctctcgga agacggactg agcttcgttg cctgcagcgc tgacgtctgc aacatcttct tccacgtgct gcagtttttc aatgaggtgc tggtagcgct gctgcctctg tttctccagt cctgcgtgca gacccgagag cctttgctcc agccggtgga gtctatccga tttgccgctg aggctcgtcg tcctcgtcgg gagagctctc gcgccttcac ttcgagaggg aagagacgaa agggggcctg acgtctgcac agacgcgaga ctagaaaagt gcgaaggatg gtctccgtga acactggcgg cggtgctgct ggcagagccg tgaccggaag ccgccgagga gagacccctc gagtgcccga gagccgtgaa tcggccgcct tccattccgt tcttcata
Expected Protein Sequence
MAHHHHHHMK NGMEGGRFTA LGHSRGLSSA ASGHGSASST AASVHGDHPS HFSSLASVQT SGPLSSLPSR SEGARALPTR TTSLSGKSDR LHRLEQRLSG LHAGLEKQRQ QRYQHLIEKL QHVEEDVADV SAAGNEAQSV FREELRKIEA DLRERQSELE TDLARREQQT QEMERRLTRM LQTEQEALRE VETSLMNAFT EKTKALREEI LQSGQLREEQ EASLRHYCEV EIPRLRDGLQ QEVRERENME RRILKHAAEE VERLNGQLAA EREARERHLQ SLLSLFEEVV REVQRDIARE KEDREKMQED LVHLMEQTID RITTSI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga acggaatgga aggcggccga ttcacggctc tcgggcactc gaggggtctc tcctcggcgg cttccggtca cggctctgcc agcagcaccg ccgccagtgt tcacggagac catccttcgc acttttctag tctcgcgtct gtgcagacgt caggccccct ttcgtctctt ccctctcgaa gtgaaggcgc gagagctctc ccgacgagga cgacgagcct cagcggcaaa tcggatagac tccaccggct ggagcaaagg ctctcgggtc tgcacgcagg actggagaaa cagaggcagc agcgctacca gcacctcatt gaaaaactgc agcacgtgga agaagatgtt gcagacgtca gcgctgcagg caacgaagct cagtccgtct tccgagagga gctgagaaaa atcgaagcag acttgcgcga gcgtcagagc gaactcgaaa ctgacttggc ccgccgagag cagcagactc aggagatgga gagaagactg acgcgcatgc tgcagacgga gcaggaggct ttacgagaag ttgaaacctc cttgatgaat gcgtttacag agaagacaaa ggctctaaga gaggaaattc ttcagagcgg ccagctgcgg gaggagcagg aggcgtcgct ccgacactac tgcgaagtcg aaattccccg cctccgcgac ggccttcagc aggaagtgcg ggaaagggaa aacatggaga gaagaattct caagcatgca gcggaggagg ttgagcgact caatgggcag cttgccgcgg agagagaagc tcgtgaacgg catctgcagt ctctgctgtc tctgttcgaa gaggtcgtcc gcgaggtgca acgcgatatc gcccgggaaa aggaggaccg agagaaaatg caggaagatc tggtccacct catggagcaa acaattgacc gcatcacaac atctatatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for TogoA.18505.a.B1.PS01606
PURIFICATION DATe: 8/23/2012
CONCENTRATION: 7.2mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 15µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MKNGMEGGRF TALGHSRGLS SAASGHGSAS STAASVHGDH PSHFSSLASV QTSGPLSSLP SRSEGARALP TRTTSLSGKS DRLHRLEQRL SGLHAGLEKQ RQQRYQHLIE KLQHVEEDVA DVSAAGNEAQ SVFREELRKI EADLRERQSE LETDLARREQ QTQEMERRLT RMLQTEQEAL REVETSLMNA FTEKTKALRE EILQSGQLRE EQEASLRHYC EVEIPRLRDG LQQEVREREN MERRILKHAA EEVERLNGQL AAEREARERH LQSLLSLFEE VVREVQRDIA REKEDREKMQ EDLVHLME
Validated NT Sequence
ttgctccatg aggtggacca gatcttcctg cattttctct cggtcctcct tttcccgggc gatatcgcgt tgcacctcgc ggacgacctc ttcgaacaga gacagcagag actgcagatg ccgttcacga gcttctctct ccgcggcaag ctgcccattg agtcgctcaa cctcctccgc tgcatgcttg agaattcttc tctccatgtt ttccctttcc cgcacttcct gctgaaggcc gtcgcggagg cggggaattt cgacttcgca gtagtgtcgg agcgacgcct cctgctcctc ccgcagctgg ccgctctgaa gaatttcctc tcttagagcc tttgtcttct ctgtaaacgc attcatcaag gaggtttcaa cttctcgtaa agcctcctgc tccgtctgca gcatgcgcgt cagtcttctc tccatctcct gagtctgctg ctctcggcgg gccaagtcag tttcgagttc gctctgacgc tcgcgcaagt ctgcttcgat ttttctcagc tcctctcgga agacggactg agcttcgttg cctgcagcgc tgacgtctgc aacatcttct tccacgtgct gcagtttttc aatgaggtgc tggtagcgct gctgcctctg tttctccagt cctgcgtgca gacccgagag cctttgctcc agccggtgga gtctatccga tttgccgctg aggctcgtcg tcctcgtcgg gagagctctc gcgccttcac ttcgagaggg aagagacgaa agggggcctg acgtctgcac agacgcgaga ctagaaaagt gcgaaggatg gtctccgtga acactggcgg cggtgctgct ggcagagccg tgaccggaag ccgccgagga gagacccctc gagtgcccga gagccgtgaa tcggccgcct tccattccgt tcttcata
Expressed Protein Sequence
MAHHHHHHMK NGMEGGRFTA LGHSRGLSSA ASGHGSASST AASVHGDHPS HFSSLASVQT SGPLSSLPSR SEGARALPTR TTSLSGKSDR LHRLEQRLSG LHAGLEKQRQ QRYQHLIEKL QHVEEDVADV SAAGNEAQSV FREELRKIEA DLRERQSELE TDLARREQQT QEMERRLTRM LQTEQEALRE VETSLMNAFT EKTKALREEI LQSGQLREEQ EASLRHYCEV EIPRLRDGLQ QEVRERENME RRILKHAAEE VERLNGQLAA EREARERHLQ SLLSLFEEVV REVQRDIARE KEDREKMQED LVHLMEQTID RITTSI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGA ACGGAATGGA AGGCGGCCGA TTCACGGCTC TCGGGCACTC GAGGGGTCTC TCCTCGGCGG CTTCCGGTCA CGGCTCTGCC AGCAGCACCG CCGCCAGTGT TCACGGAGAC CATCCTTCGC ACTTTTCTAG TCTCGCGTCT GTGCAGACGT CAGGCCCCCT TTCGTCTCTT CCCTCTCGAA GTGAAGGCGC GAGAGCTCTC CCGACGAGGA CGACGAGCCT CAGCGGCAAA TCGGATAGAC TCCACCGGCT GGAGCAAAGG CTCTCGGGTC TGCACGCAGG ACTGGAGAAA CAGAGGCAGC AGCGCTACCA GCACCTCATT GAAAAACTGC AGCACGTGGA AGAAGATGTT GCAGACGTCA GCGCTGCAGG CAACGAAGCT CAGTCCGTCT TCCGAGAGGA GCTGAGAAAA ATCGAAGCAG ACTTGCGCGA GCGTCAGAGC GAACTCGAAA CTGACTTGGC CCGCCGAGAG CAGCAGACTC AGGAGATGGA GAGAAGACTG ACGCGCATGC TGCAGACGGA GCAGGAGGCT TTACGAGAAG TTGAAACCTC CTTGATGAAT GCGTTTACAG AGAAGACAAA GGCTCTAAGA GAGGAAATTC TTCAGAGCGG CCAGCTGCGG GAGGAGCAGG AGGCGTCGCT CCGACACTAC TGCGAAGTCG AAATTCCCCG CCTCCGCGAC GGCCTTCAGC AGGAAGTGCG GGAAAGGGAA AACATGGAGA GAAGAATTCT CAAGCATGCA GCGGAGGAGG TTGAGCGACT CAATGGGCAG CTTGCCGCGG AGAGAGAAGC TCGTGAACGG CATCTGCAGT CTCTGCTGTC TCTGTTCGAA GAGGTCGTCC GCGAGGTGCA ACGCGATATC GCCCGGGAAA AGGAGGACCG AGAGAAAATG CAGGAAGATC TGGTCCACCT CATGGAGCAA ACAATTGACC GCATCACAAC ATCTATATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT