TogoA.18347.a

Myosin-A (TgM-A)

CENTER ID: TogoA.18347.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.18347.a.B1.GE37246 full length 1 831
TogoA.18347.a.B2.GE37247 P-loop with NTP hydrolases 67 818
TogoA.18347.a.B3.GE37248 myosin domain 106 761
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:O00934
UniProt: O00934

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASKTTSEEL KTATALKKRS SDVHAVDHSG NVYKGFQIWT DLAPSVKEEP DLMFAKCIVQ AGTDKGNLTC VQIDPPGFDE PFEVPQANAW NVNSLIDPMT YGDIGMLPHT NIPCVLDFLK VRFMKNQIYT TADPLVVAIN PFRDLGNTTL DWIVRYRDTF DLSKLAPHVF YTARRALDNL HAVNKSQTII VSGESGAGKT EATKQIMRYF AAAKTGSMDL RIQNAIMAAN PVLEAFGNAK TIRNNNSSRF GRFMQLDVGR EGGIKFGSVV AFLLEKSRVL TQDEQERSYH IFYQMCKGAD AAMKERFHIL PLSEYKYINP LCLDAPGIDD VAEFHEVCES FRSMNLTEDE VASVWSIVSG VLLLGNVEVT ATKDGGIDDA AAIEGKNLEV FKKACGLLFL DAERIREELT VKVSYAGNQE IRGRWKQEDG DMLKSSLAKA MYDKLFMWII AVLNRSIKPP GGFKIFMGML DIFGFEVFKN NSLEQFFINI TNEMLQKNFV DIVFDRESKL YRDEGVSSKE LIFTSNAEVI KILTAKNNSV LAALEDQCLA PGGSDEKFLS TCKNALKGTT KFKPAKVSPN INFLISHTVG DIQYNAEGFL FKNKDVLRAE IMEIVQQSKN PVVAQLFAGI VMEKGKMAKG QLIGSQFLSQ LQSLMELINS TEPHFIRCIK PNDTKKPLDW VPSKMLIQLH ALSVLEALQL RQLGYSYRRP FKEFLFQFKF IDLSASENPN LDPKEAALRL LKSSKLPSEE YQLGKTMVFL KQTGAKELTQ IQRECLSSWE PLVSVLEAYY AGRRHKKQLL KKTPFIIRAQ AHIRRHLVDN NVSPATVQPA F
NT Sequence
atggcgagca agaccacgtc tgaggagctg aaaacggcca cggcgctgaa gaagaggtcg tccgatgtcc acgcggtcga ccactccggc aatgtgtaca aaggatttca aatctggacg gacttggcgc cgtcggtgaa ggaggagccg gacctgatgt ttgccaagtg catcgtgcag gcggggacag acaaggggaa cttgacctgc gtccagatcg atccaccggg cttcgacgaa ccgttcgaag tcccgcaggc gaatgcgtgg aacgtaaaca gcctgatcga ccccatgacg tacggagaca tcggcatgtt gcctcacacg aacattcctt gcgtcctcga cttcctcaag gtgcgcttca tgaagaatca aatctacacg actgcggacc cgctcgtcgt cgccatcaat cccttccgcg acctcgggaa caccacgctc gactggattg ttcgatacag agacactttc gacctctcca aactcgcgcc ccatgttttc tacaccgccc gacgcgcgct cgacaacctc cacgccgtca acaagtcgca aacgatcatc gtgtccggtg agtctggcgc gggcaagacg gaggcgacga agcagattat gaggtatttt gcggcggcga agacggggtc gatggatttg cggattcaga acgcgatcat ggcggcgaat ccagtgcttg aggcatttgg aaatgcgaag acgattcgca acaacaactc gtcgcgtttc ggacgcttca tgcagctgga tgtgggtcgc gaaggaggca tcaagtttgg ctccgtcgtc gcctttctcc tggaaaagtc gcgtgttctc acgcaggacg aacaggagcg gtcgtaccac atcttctacc aaatgtgcaa gggggcggac gcggcgatga aggagcgctt ccatatcctg ccgctctcgg agtacaagta catcaatccg ttgtgcctgg acgcgccagg gatcgacgac gtcgcggagt tccacgaagt ctgcgagtcg ttccggtcga tgaatctgac ggaggacgaa gtcgcgagcg tgtggagcat cgtgagtgga gtgctgctgc ttggcaacgt cgaggtgaca gcgacgaagg atggggggat cgacgacgcc gcggcgatcg aggggaagaa cttggaggtt ttcaaaaagg cctgcgggct gctcttcctc gacgcggagc gcattcgcga agagctgacg gtgaaggttt cgtatgcggg gaatcaggag atccgcggcc ggtggaagca ggaagacgga gacatgctca agtcgtcgct cgcgaaggcg atgtacgaca agttgttcat gtggatcatt gccgtgttga accgcagcat caagcctccg ggcggcttca agatcttcat gggcatgctc gacatcttcg gcttcgaagt cttcaagaac aactcgctgg agcagttctt catcaacatc acgaacgaaa tgctgcagaa gaacttcgtc gacatcgtct tcgaccgcga gagcaagctg tatcgtgacg agggtgtctc ctccaaggag ttgattttca cctcgaacgc agaagtgatc aagatcttga cggcgaagaa caactcggtg ctcgctgcgc tcgaggacca gtgcctcgcc cctggaggca gcgacgaaaa gttcctctcg acctgcaaga acgcgctgaa aggaaccacc aagttcaagc ctgcgaaggt ctctccgaac atcaatttcc tcatctcgca cactgtcggc gacatccagt acaacgccga aggcttcctc ttcaaaaaca aagatgtcct gcgagcagaa atcatggaaa tcgtgcagca aagcaagaac cccgttgtcg cgcaactctt cgctggcatc gtcatggaga aggggaagat ggccaaggga caactgattg ggtcgcagtt cctctcgcag ctgcagagcc tcatggaact tatcaacagc accgagcctc acttcattcg ctgcatcaag ccgaacgaca cgaagaagcc cctcgactgg gtgccgtcga aaatgctcat tcagctgcac gcgctctccg tcctcgaggc tcttcagctc cgtcaactcg gctactctta cagacgtccg ttcaaggagt tcctcttcca gttcaagttt atcgacctct cggcttctga aaatccaaat ctggacccca aagaagctgc gctgagactc ctcaaaagca gcaaactgcc cagcgaagaa taccagctcg ggaagacaat ggttttcctc aagcagacgg gcgcgaaaga actgacgcag attcagagag aatgcctttc ttcttgggag cctctcgtct cagtgctcga ggcgtactac gctggcagac gccacaagaa gcagctgctg aaaaagaccc ccttcatcat tcgcgcccag gctcacatcc gcagacacct ggtggacaac aacgtcagcc ccgcgactgt tcagccggcg ttctag
Details for TogoA.18347.a.B1.GE37246
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 94kDa
OBSERVED MW: 94kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 97
PERCENT COVERAGE: 36
Validated AA Sequence
MAHHHHHHMA SKTTSEELKT ATALKKRSSD VHAVDHSGNV YKGFQIWTDL APSVKEEPDL MFAKCIVQAG TDKGNLTCVQ IDPPGFDEPF EVPQANAWNV NSLIDPMTYG DIGMLPHTNI PCVLDFLKVR FMKNQIYTTA DPLVVAINPF RDLGNTTLDW IVRYRDTFDL SKLAPHVFYT ARRALDNLHA VNKSQTIIVS GESGAGKTEA TKQIMRYFAA AKTGSMDLRI QNAIMAANPV LEAFGNAKTI RNNNSSRFGR FMQLDVGREX GIXFGSVVAF LLEKSRVLTX XEQERSYHIF YQMCXGRXRR
Validated NT Sequence
tttgttnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatggcgag caagaccacg tctgaggagc tgaaaacggc cacggcgctg aagaagaggt cgtccgatgt ccacgcggtc gaccactccg gcaatgtgta caaaggattt caaatctgga cggacctggc gccgtcggtg aaggaggagc cggacctgat gtttgccaag tgcatcgtgc aggcggggac agacaagggg aacttgacct gcgtccagat cgatccaccg ggcttcgacg aaccgttcga agtaccgcag gcgaatgcgt ggaatgtgaa cagcctgatc gaccccatga cgtacggaga catcggcatg ttgcctcaca cgaacattcc ttgcgtcctc gacttcctca aggtgcgctt catgaagaat caaatctaca cgactgcgga cccgctcgtc gttgccatca atcccttccg cgacctcggg aacaccacgc tcgactggat tgttcgatac agagacactt tcgacctctc caaactcgcg ccccatgttt tctacaccgc ccgacgcgcg ctcgacaacc tccacgccgt caacaagtcg caaacgatca tcgtgtccgg tgagtctggc gcgggcaaga cagaggcgac gaagcagatt atgaggtatt ttgcggcggc gaagacgggg tcgatggatt tgcggattca gaacgcgatc atggcggcga atccagtgct tgaggcattt ggaaatgcga agacgattcg caacaacaac tcgtcgcgtt tcggacgctt catgcagctg gatgtgggtc gcgaangagg catcnagttt ggctccgtcg tcgcctttct cctggaaaag tcgcgtgttc tcacgcngga ngaacaggag cggtcgtacc acatcttcta ccaaatgtgc anggggcgga ngcggcgatg aagnancgct tncatatcct gccgctctcg gagtananta catcaatccg tnnngnctgg ncgcncnnnc gacnacnncn nnnantnccn cnaagtcngn nnntcnnnnn ncgaanctga ngnannanna nnnnnnnnnn nnnnnnncat nnnnnnnnng nnnctnnnnn nnnnnnnnnn nnnnnnnnnn annnnnnngg nnnnnnnnng nnnnnnnnnn nnnnnnannc nnnnngnnnn nnnnnnnnnt
Expected Protein Sequence
MAHHHHHHMA SKTTSEELKT ATALKKRSSD VHAVDHSGNV YKGFQIWTDL APSVKEEPDL MFAKCIVQAG TDKGNLTCVQ IDPPGFDEPF EVPQANAWNV NSLIDPMTYG DIGMLPHTNI PCVLDFLKVR FMKNQIYTTA DPLVVAINPF RDLGNTTLDW IVRYRDTFDL SKLAPHVFYT ARRALDNLHA VNKSQTIIVS GESGAGKTEA TKQIMRYFAA AKTGSMDLRI QNAIMAANPV LEAFGNAKTI RNNNSSRFGR FMQLDVGREG GIKFGSVVAF LLEKSRVLTQ DEQERSYHIF YQMCKGADAA MKERFHILPL SEYKYINPLC LDAPGIDDVA EFHEVCESFR SMNLTEDEVA SVWSIVSGVL LLGNVEVTAT KDGGIDDAAA IEGKNLEVFK KACGLLFLDA ERIREELTVK VSYAGNQEIR GRWKQEDGDM LKSSLAKAMY DKLFMWIIAV LNRSIKPPGG FKIFMGMLDI FGFEVFKNNS LEQFFINITN EMLQKNFVDI VFDRESKLYR DEGVSSKELI FTSNAEVIKI LTAKNNSVLA ALEDQCLAPG GSDEKFLSTC KNALKGTTKF KPAKVSPNIN FLISHTVGDI QYNAEGFLFK NKDVLRAEIM EIVQQSKNPV VAQLFAGIVM EKGKMAKGQL IGSQFLSQLQ SLMELINSTE PHFIRCIKPN DTKKPLDWVP SKMLIQLHAL SVLEALQLRQ LGYSYRRPFK EFLFQFKFID LSASENPNLD PKEAALRLLK SSKLPSEEYQ LGKTMVFLKQ TGAKELTQIQ RECLSSWEPL VSVLEAYYAG RRHKKQLLKK TPFIIRAQAH IRRHLVDNNV SPATVQPAF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga gcaagaccac gtctgaggag ctgaaaacgg ccacggcgct gaagaagagg tcgtccgatg tccacgcggt cgaccactcc ggcaatgtgt acaaaggatt tcaaatctgg acggacttgg cgccgtcggt gaaggaggag ccggacctga tgtttgccaa gtgcatcgtg caggcgggga cagacaaggg gaacttgacc tgcgtccaga tcgatccacc gggcttcgac gaaccgttcg aagtcccgca ggcgaatgcg tggaacgtaa acagcctgat cgaccccatg acgtacggag acatcggcat gttgcctcac acgaacattc cttgcgtcct cgacttcctc aaggtgcgct tcatgaagaa tcaaatctac acgactgcgg acccgctcgt cgtcgccatc aatcccttcc gcgacctcgg gaacaccacg ctcgactgga ttgttcgata cagagacact ttcgacctct ccaaactcgc gccccatgtt ttctacaccg cccgacgcgc gctcgacaac ctccacgccg tcaacaagtc gcaaacgatc atcgtgtccg gtgagtctgg cgcgggcaag acggaggcga cgaagcagat tatgaggtat tttgcggcgg cgaagacggg gtcgatggat ttgcggattc agaacgcgat catggcggcg aatccagtgc ttgaggcatt tggaaatgcg aagacgattc gcaacaacaa ctcgtcgcgt ttcggacgct tcatgcagct ggatgtgggt cgcgaaggag gcatcaagtt tggctccgtc gtcgcctttc tcctggaaaa gtcgcgtgtt ctcacgcagg acgaacagga gcggtcgtac cacatcttct accaaatgtg caagggggcg gacgcggcga tgaaggagcg cttccatatc ctgccgctct cggagtacaa gtacatcaat ccgttgtgcc tggacgcgcc agggatcgac gacgtcgcgg agttccacga agtctgcgag tcgttccggt cgatgaatct gacggaggac gaagtcgcga gcgtgtggag catcgtgagt ggagtgctgc tgcttggcaa cgtcgaggtg acagcgacga aggatggggg gatcgacgac gccgcggcga tcgaggggaa gaacttggag gttttcaaaa aggcctgcgg gctgctcttc ctcgacgcgg agcgcattcg cgaagagctg acggtgaagg tttcgtatgc ggggaatcag gagatccgcg gccggtggaa gcaggaagac ggagacatgc tcaagtcgtc gctcgcgaag gcgatgtacg acaagttgtt catgtggatc attgccgtgt tgaaccgcag catcaagcct ccgggcggct tcaagatctt catgggcatg ctcgacatct tcggcttcga agtcttcaag aacaactcgc tggagcagtt cttcatcaac atcacgaacg aaatgctgca gaagaacttc gtcgacatcg tcttcgaccg cgagagcaag ctgtatcgtg acgagggtgt ctcctccaag gagttgattt tcacctcgaa cgcagaagtg atcaagatct tgacggcgaa gaacaactcg gtgctcgctg cgctcgagga ccagtgcctc gcccctggag gcagcgacga aaagttcctc tcgacctgca agaacgcgct gaaaggaacc accaagttca agcctgcgaa ggtctctccg aacatcaatt tcctcatctc gcacactgtc ggcgacatcc agtacaacgc cgaaggcttc ctcttcaaaa acaaagatgt cctgcgagca gaaatcatgg aaatcgtgca gcaaagcaag aaccccgttg tcgcgcaact cttcgctggc atcgtcatgg agaaggggaa gatggccaag ggacaactga ttgggtcgca gttcctctcg cagctgcaga gcctcatgga acttatcaac agcaccgagc ctcacttcat tcgctgcatc aagccgaacg acacgaagaa gcccctcgac tgggtgccgt cgaaaatgct cattcagctg cacgcgctct ccgtcctcga ggctcttcag ctccgtcaac tcggctactc ttacagacgt ccgttcaagg agttcctctt ccagttcaag tttatcgacc tctcggcttc tgaaaatcca aatctggacc ccaaagaagc tgcgctgaga ctcctcaaaa gcagcaaact gcccagcgaa gaataccagc tcgggaagac aatggttttc ctcaagcaga cgggcgcgaa agaactgacg cagattcaga gagaatgcct ttcttcttgg gagcctctcg tctcagtgct cgaggcgtac tacgctggca gacgccacaa gaagcagctg ctgaaaaaga cccccttcat cattcgcgcc caggctcaca tccgcagaca cctggtggac aacaacgtca gccccgcgac tgttcagccg gcgttctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for TogoA.18347.a.B2.GE37247
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 86kDa
OBSERVED MW: 86kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 50
Validated AA Sequence
MAHHHHHHMN LTCVQIDPPG FDEPFEVPQA NAWNVNSLID PMTYGDIGML PHTNIPCVLD FLKVRFMKNQ IYTTADPLVV AINPFRDLGN TTLDWIVRYR DTFDLSKLAP HVFYTARRAL DNLHAVNKSQ TIIVSGESGA GKTEATKQIM RYFAAAKTGS MDLRIQNAIM AANPVLEAFG NAKTIRNNNS SRFGRFMQLD VGREGGIKFG SVVAFLLEKS RVLTQDEQER SYHIFYQMCK GADAAMXERF HILPLSXYKY INPLCLDAPG IDDVAEFHEV CESFRSMNLX XDEXASVWSI VSGVLLLGNV XVTATXXGGI DXAXAIEGXN LXVFKXXXXC XSSTXXXXXX XXGXXXXXXS XXPXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
attttgttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgaac ttgacctgcg tccagatcga tccaccgggc ttcgacgaac cgttcgaagt accgcaggcg aatgcgtgga atgtgaacag cctgatcgac cccatgacgt acggagacat cggcatgttg cctcacacga acattccttg cgtcctcgac ttcctcaagg tgcgcttcat gaagaatcaa atctacacga ctgcggaccc gctcgtcgtt gccatcaatc ccttccgcga cctcgggaac accacgctcg actggattgt tcgatacaga gacactttcg acctctccaa actcgcgccc catgttttct acaccgcccg acgcgcgctc gacaacctcc acgccgtcaa caagtcgcaa acgatcatcg tgtccggtga gtctggcgcg ggcaagacag aggcgacgaa gcagattatg aggtattttg cggcggcgaa gacggggtcg atggatttgc ggattcagaa cgcgatcatg gcggcgaatc cagtgcttga ggcatttgga aatgcgaaga cgattcgcaa caacaactcg tcgcgtttcg gacgcttcat gcagctggat gtgggtcgcg aaggaggcat caagtttggc tccgtcgtcg cctttctcct ggaaaagtcg cgtgttctca cgcaggacga acaggagcgg tcgtaccaca tcttctacca aatgtgcaag ggggcggacg cggcgatgaa ngagcgcttc catatcctgc cgctctcgga ntacaagtac atcaatccgt tgtgcctgga cgcgccaggg atcgacgacg tcgcggagtt ccacgaagtc tgcgagtcgt tccggtcgat gaatctgang gangacgaan tcgcgagcgt gtggagcatc gtgagtggag tgctgctgct cggcaacgtc naggtgacng cgacgaanna tggggggatc gacnacgcnn cngcgatcga ggggangaac ttggnngttt tcaaaaannn tgnngnctgc nnntcctcga cnnnnannnc ntnncnaanc tgnnngggnn nnntnnnnat nnggnnntcn nnnnntccnn nnnngnnnnn gnnnnnnnnn nnnnnnnaan nnnannnnng gnngnnnnnn nnnannnnnt nnnnnng
Expected Protein Sequence
MAHHHHHHNL TCVQIDPPGF DEPFEVPQAN AWNVNSLIDP MTYGDIGMLP HTNIPCVLDF LKVRFMKNQI YTTADPLVVA INPFRDLGNT TLDWIVRYRD TFDLSKLAPH VFYTARRALD NLHAVNKSQT IIVSGESGAG KTEATKQIMR YFAAAKTGSM DLRIQNAIMA ANPVLEAFGN AKTIRNNNSS RFGRFMQLDV GREGGIKFGS VVAFLLEKSR VLTQDEQERS YHIFYQMCKG ADAAMKERFH ILPLSEYKYI NPLCLDAPGI DDVAEFHEVC ESFRSMNLTE DEVASVWSIV SGVLLLGNVE VTATKDGGID DAAAIEGKNL EVFKKACGLL FLDAERIREE LTVKVSYAGN QEIRGRWKQE DGDMLKSSLA KAMYDKLFMW IIAVLNRSIK PPGGFKIFMG MLDIFGFEVF KNNSLEQFFI NITNEMLQKN FVDIVFDRES KLYRDEGVSS KELIFTSNAE VIKILTAKNN SVLAALEDQC LAPGGSDEKF LSTCKNALKG TTKFKPAKVS PNINFLISHT VGDIQYNAEG FLFKNKDVLR AEIMEIVQQS KNPVVAQLFA GIVMEKGKMA KGQLIGSQFL SQLQSLMELI NSTEPHFIRC IKPNDTKKPL DWVPSKMLIQ LHALSVLEAL QLRQLGYSYR RPFKEFLFQF KFIDLSASEN PNLDPKEAAL RLLKSSKLPS EEYQLGKTMV FLKQTGAKEL TQIQRECLSS WEPLVSVLEA YYAGRRHKKQ LLKKTPFIIR AQAHIRRHLV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataacttga cctgcgtcca gatcgatcca ccgggcttcg acgaaccgtt cgaagtcccg caggcgaatg cgtggaacgt aaacagcctg atcgacccca tgacgtacgg agacatcggc atgttgcctc acacgaacat tccttgcgtc ctcgacttcc tcaaggtgcg cttcatgaag aatcaaatct acacgactgc ggacccgctc gtcgtcgcca tcaatccctt ccgcgacctc gggaacacca cgctcgactg gattgttcga tacagagaca ctttcgacct ctccaaactc gcgccccatg ttttctacac cgcccgacgc gcgctcgaca acctccacgc cgtcaacaag tcgcaaacga tcatcgtgtc cggtgagtct ggcgcgggca agacggaggc gacgaagcag attatgaggt attttgcggc ggcgaagacg gggtcgatgg atttgcggat tcagaacgcg atcatggcgg cgaatccagt gcttgaggca tttggaaatg cgaagacgat tcgcaacaac aactcgtcgc gtttcggacg cttcatgcag ctggatgtgg gtcgcgaagg aggcatcaag tttggctccg tcgtcgcctt tctcctggaa aagtcgcgtg ttctcacgca ggacgaacag gagcggtcgt accacatctt ctaccaaatg tgcaaggggg cggacgcggc gatgaaggag cgcttccata tcctgccgct ctcggagtac aagtacatca atccgttgtg cctggacgcg ccagggatcg acgacgtcgc ggagttccac gaagtctgcg agtcgttccg gtcgatgaat ctgacggagg acgaagtcgc gagcgtgtgg agcatcgtga gtggagtgct gctgcttggc aacgtcgagg tgacagcgac gaaggatggg gggatcgacg acgccgcggc gatcgagggg aagaacttgg aggttttcaa aaaggcctgc gggctgctct tcctcgacgc ggagcgcatt cgcgaagagc tgacggtgaa ggtttcgtat gcggggaatc aggagatccg cggccggtgg aagcaggaag acggagacat gctcaagtcg tcgctcgcga aggcgatgta cgacaagttg ttcatgtgga tcattgccgt gttgaaccgc agcatcaagc ctccgggcgg cttcaagatc ttcatgggca tgctcgacat cttcggcttc gaagtcttca agaacaactc gctggagcag ttcttcatca acatcacgaa cgaaatgctg cagaagaact tcgtcgacat cgtcttcgac cgcgagagca agctgtatcg tgacgagggt gtctcctcca aggagttgat tttcacctcg aacgcagaag tgatcaagat cttgacggcg aagaacaact cggtgctcgc tgcgctcgag gaccagtgcc tcgcccctgg aggcagcgac gaaaagttcc tctcgacctg caagaacgcg ctgaaaggaa ccaccaagtt caagcctgcg aaggtctctc cgaacatcaa tttcctcatc tcgcacactg tcggcgacat ccagtacaac gccgaaggct tcctcttcaa aaacaaagat gtcctgcgag cagaaatcat ggaaatcgtg cagcaaagca agaaccccgt tgtcgcgcaa ctcttcgctg gcatcgtcat ggagaagggg aagatggcca agggacaact gattgggtcg cagttcctct cgcagctgca gagcctcatg gaacttatca acagcaccga gcctcacttc attcgctgca tcaagccgaa cgacacgaag aagcccctcg actgggtgcc gtcgaaaatg ctcattcagc tgcacgcgct ctccgtcctc gaggctcttc agctccgtca actcggctac tcttacagac gtccgttcaa ggagttcctc ttccagttca agtttatcga cctctcggct tctgaaaatc caaatctgga ccccaaagaa gctgcgctga gactcctcaa aagcagcaaa ctgcccagcg aagaatacca gctcgggaag acaatggttt tcctcaagca gacgggcgcg aaagaactga cgcagattca gagagaatgc ctttcttctt gggagcctct cgtctcagtg ctcgaggcgt actacgctgg cagacgccac aagaagcagc tgctgaaaaa gacccccttc atcattcgcg cccaggctca catccgcaga cacctggtgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for TogoA.18347.a.B3.GE37248
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 75kDa
OBSERVED MW: 75kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 77
PERCENT COVERAGE: 60
Validated AA Sequence
MAHHHHHHHM LPHTNIPCVL DFLKVRFMKN QIYTTADPLV VAINPFRDLG NTTLDWIVRY RDTFDLSKLA PHVFYTARRA LDNLHAVNKS QTIIVSGESG AGKTEATKQI MRYFAAAKTG SMDLRIQNAI MAANPVLEAF GNAKTIRNNN SSRFGRFMQL DVGREGGIKF GSVVAFLLEK SRVLTQDEQE RSYHIFYQMC KGADAAMKER FHILPLSEYK YINPLCLDAP GIDDVAEFHE VCESFRSMNL TEDEVASVWS IVSGVLLLGN VXVTATXXGG IDDAAAIEGK NLEVFKKXCG LLFLDAXRIR XXLXGXVSYX GXXEIXGRXX QXXXXCXXXA RXXXXRXXXA XXXXXXSAXG XXXXXXXXXT XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
ttttgtttac tttaagaagg aganatacca tggctcacca ccaccaccac caccatatgt tgcctcacac gaacattcct tgcgtcctcg acttcctcaa ggtgcgcttc atgaagaatc aaatctacac gactgcggac ccgctcgtcg ttgccatcaa tcccttccgc gacctcggga acaccacgct cgactggatt gttcgataca gagacacttt cgacctctcc aaactcgcgc cccatgtttt ctacaccgcc cgacgcgcgc tcgacaacct ccacgccgtc aacaagtcgc aaacgatcat cgtgtccggt gagtctggcg cgggcaagac agaggcgacg aagcagatta tgaggtattt tgcggcggcg aagacggggt cgatggattt gcggattcag aacgcgatca tggcggcgaa tccagtgctt gaggcatttg gaaatgcgaa gacgattcgc aacaacaact cgtcgcgttt cggacgcttc atgcagctgg atgtgggtcg cgaaggaggc atcaagtttg gctccgtcgt cgcctttctc ctggaaaagt cgcgtgttct cacgcaggac gaacaggagc ggtcgtacca catcttctac caaatgtgca agggggcgga cgcggcgatg aaggagcgct tccatatcct gccgctctcg gagtacaagt acatcaatcc gttgtgcctg gacgcgccag ggatcgacga cgtcgcggag ttccacgaag tctgcgagtc gttccggtcg atgaatctga cggaggacga agtcgcgagc gtgtggagca tcgtgagtgg agtgctgctg ctcggcaacg tcgangtgac agcgacgaan nntgggggga tcgacgacgc cgcggcgatc gaggggaaga acttggaggt tttcaaaaag gnctgcgggc tgctcttcct cgacgcgnnn cgcattcgcg annanctgan gggganggtt tcgtatgngg ggnatcngga gatccncggc cggnngnagc aggannangn aanntgctna gnnntcgctc gcnangnnna tgnacganan nnnnntgcat nnncnnnnnn nncancatca gcntnnggnn gnnncannnn ntnangggnn tnnncgacnn nntnnnnnnn nannnnnnnn naannnnnnn nngnnnnnnn tnnnnnaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnan ntnnnnnnnn nnnnnnnnnn nnnnt
Expected Protein Sequence
MAHHHHHHML PHTNIPCVLD FLKVRFMKNQ IYTTADPLVV AINPFRDLGN TTLDWIVRYR DTFDLSKLAP HVFYTARRAL DNLHAVNKSQ TIIVSGESGA GKTEATKQIM RYFAAAKTGS MDLRIQNAIM AANPVLEAFG NAKTIRNNNS SRFGRFMQLD VGREGGIKFG SVVAFLLEKS RVLTQDEQER SYHIFYQMCK GADAAMKERF HILPLSEYKY INPLCLDAPG IDDVAEFHEV CESFRSMNLT EDEVASVWSI VSGVLLLGNV EVTATKDGGI DDAAAIEGKN LEVFKKACGL LFLDAERIRE ELTVKVSYAG NQEIRGRWKQ EDGDMLKSSL AKAMYDKLFM WIIAVLNRSI KPPGGFKIFM GMLDIFGFEV FKNNSLEQFF INITNEMLQK NFVDIVFDRE SKLYRDEGVS SKELIFTSNA EVIKILTAKN NSVLAALEDQ CLAPGGSDEK FLSTCKNALK GTTKFKPAKV SPNINFLISH TVGDIQYNAE GFLFKNKDVL RAEIMEIVQQ SKNPVVAQLF AGIVMEKGKM AKGQLIGSQF LSQLQSLMEL INSTEPHFIR CIKPNDTKKP LDWVPSKMLI QLHALSVLEA LQLRQLGYSY RRPFKEFLFQ FKFIDLSASE NPNLDPKEAA LRLLKSSKLP SEEYQLGKTM VFLK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgc ctcacacgaa cattccttgc gtcctcgact tcctcaaggt gcgcttcatg aagaatcaaa tctacacgac tgcggacccg ctcgtcgtcg ccatcaatcc cttccgcgac ctcgggaaca ccacgctcga ctggattgtt cgatacagag acactttcga cctctccaaa ctcgcgcccc atgttttcta caccgcccga cgcgcgctcg acaacctcca cgccgtcaac aagtcgcaaa cgatcatcgt gtccggtgag tctggcgcgg gcaagacgga ggcgacgaag cagattatga ggtattttgc ggcggcgaag acggggtcga tggatttgcg gattcagaac gcgatcatgg cggcgaatcc agtgcttgag gcatttggaa atgcgaagac gattcgcaac aacaactcgt cgcgtttcgg acgcttcatg cagctggatg tgggtcgcga aggaggcatc aagtttggct ccgtcgtcgc ctttctcctg gaaaagtcgc gtgttctcac gcaggacgaa caggagcggt cgtaccacat cttctaccaa atgtgcaagg gggcggacgc ggcgatgaag gagcgcttcc atatcctgcc gctctcggag tacaagtaca tcaatccgtt gtgcctggac gcgccaggga tcgacgacgt cgcggagttc cacgaagtct gcgagtcgtt ccggtcgatg aatctgacgg aggacgaagt cgcgagcgtg tggagcatcg tgagtggagt gctgctgctt ggcaacgtcg aggtgacagc gacgaaggat ggggggatcg acgacgccgc ggcgatcgag gggaagaact tggaggtttt caaaaaggcc tgcgggctgc tcttcctcga cgcggagcgc attcgcgaag agctgacggt gaaggtttcg tatgcgggga atcaggagat ccgcggccgg tggaagcagg aagacggaga catgctcaag tcgtcgctcg cgaaggcgat gtacgacaag ttgttcatgt ggatcattgc cgtgttgaac cgcagcatca agcctccggg cggcttcaag atcttcatgg gcatgctcga catcttcggc ttcgaagtct tcaagaacaa ctcgctggag cagttcttca tcaacatcac gaacgaaatg ctgcagaaga acttcgtcga catcgtcttc gaccgcgaga gcaagctgta tcgtgacgag ggtgtctcct ccaaggagtt gattttcacc tcgaacgcag aagtgatcaa gatcttgacg gcgaagaaca actcggtgct cgctgcgctc gaggaccagt gcctcgcccc tggaggcagc gacgaaaagt tcctctcgac ctgcaagaac gcgctgaaag gaaccaccaa gttcaagcct gcgaaggtct ctccgaacat caatttcctc atctcgcaca ctgtcggcga catccagtac aacgccgaag gcttcctctt caaaaacaaa gatgtcctgc gagcagaaat catggaaatc gtgcagcaaa gcaagaaccc cgttgtcgcg caactcttcg ctggcatcgt catggagaag gggaagatgg ccaagggaca actgattggg tcgcagttcc tctcgcagct gcagagcctc atggaactta tcaacagcac cgagcctcac ttcattcgct gcatcaagcc gaacgacacg aagaagcccc tcgactgggt gccgtcgaaa atgctcattc agctgcacgc gctctccgtc ctcgaggctc ttcagctccg tcaactcggc tactcttaca gacgtccgtt caaggagttc ctcttccagt tcaagtttat cgacctctcg gcttctgaaa atccaaatct ggaccccaaa gaagctgcgc tgagactcct caaaagcagc aaactgccca gcgaagaata ccagctcggg aagacaatgg ttttcctcaa gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat