SSGCID: Target Page

TogoA.18104.a

Subtilase family serine protease, putative (EC 3.4.21.62) (EC 3.4.24.35)

CENTER ID:	TogoA.18104.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.18104.a.A2.GE33576	domain 1		56	550
TogoA.18104.a.A3.GE33577	domain 2		551	761

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.18104.a.A2.PS01474	domain 1		56	550

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_204050
RefSeq:	XP_002367687.1
UniProt:	B6KI42

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGSSHAIVAC AALIVLLSTN ARGLRVRKDK DVLLATSFLS HHGEYQNPTG TYNLIKEIRR VEAEIEDEVE TLNRDRRLHR GHNKYADDDI RQGLKGEQDM GASENIPVAE LEPQDLDREA KYPVRMLIVD KRSDDDDEET KTSFVETALH SDLAQRVVKE LNGHVDVLRE SGVVLVDLPA QTTDKQLQEL IETARAQGTI VEPDHLVQSV NTSSKGSNDP LLDRLWGMDA LNVKEAWDII TTGEPNMGGR RPLVCVLDTG IDYNHPDLRD NMEVNQAERD GTLGVDDDNN GEVDDIYGAN MLSKENDPAD DHSHGTHVAG TIGAHGNNGI GVAGVAWAPR LLPCKFLAYT GRGYSSDAVR CIDYCVKRGA DIVNHSWGGS WPSEALREAV VRTANNGLIH IFAAGNDGVD IDQTAFYPAA FSTEADGLIT VANVKGDPDH GGKRIIELDR SSNYGIQRVQ VACPGMWILS TVPTSGSSQE PYAEKSGTSM AAPALSGIVA LMLAVNPGLS TRQVREGLRQ CSVQQPLLQG KVEWGSMPDA KRCVEYALTT HAEGRHKSFI REPSTETSTP PPPPPAQPTP QPQPHPPPQP ETPPCAPSPP PPTPPCAPSP SPPTPPPGSP HKPEPQTPVY PEVPRSTRSP PPSPPPTESA PEAPPSDTPS CRVPPCSSSP RSGSQPKPPQ DNTTTPKMPS LSSPPTEHST AQPPKHENDA REQEPPTDED DFSSVKGKKL GAYESDGSPR ASSCAGAGVL GVFFMVVGLT V

NT Sequence

atggggtcat ctcatgccat tgtcgcgtgt gctgccttga tcgtgttgtt atccaccaac gcgagaggac tcagggtgcg gaaagacaaa gacgtactgt tggcaacgag ttttctttcc caccatggag aatatcagaa tcccactggc acatataacc ttatcaagga gattcggagg gtggaagcgg aaatcgaaga tgaggttgaa acactaaaca gagacagaag gctgcatcgt gggcacaaca aatatgccga tgacgacatc cgacaggggc taaagggcga gcaagatatg ggcgcttcag aaaatattcc cgttgcggag ctcgaaccac aggatttaga tcgggaggcg aagtatcctg tgcggatgtt gattgtggac aagcgttctg atgatgatga tgaagagacc aaaacctcgt ttgtggagac ggcgctacat tcggatcttg cgcaacgcgt tgtaaaggag ctgaacgggc atgtggacgt ccttagagaa agtggcgtgg ttttagtgga tcttccagca caaaccaccg acaaacaact gcaggaattg atcgaaaccg ccagggcaca aggcaccata gtagagccgg atcacttggt tcaaagtgtc aatacatcgt cgaaaggatc aaatgaccca cttctggatc gtctctgggg aatggatgcg ttgaacgtga aagaggcttg ggacatcatc actaccggcg agcctaatat ggggggtcgg aggccactag tttgtgtgtt agatactggc atcgattata atcatccaga cttacgggac aacatggaag tgaaccaggc agagagggac ggcacgcttg gagttgacga cgacaataac ggtgaggttg acgacatcta cggggccaac atgctcagca aggaaaacga ccctgcggat gatcactctc acggcactca tgttgccgga accatcggtg ctcacggaaa caacggtata ggagttgctg gtgttgcatg ggcgccccgt ctgcttccgt gcaaattcct cgcctataca ggaagaggat acagctcgga tgcagtccgc tgcatcgact actgcgtcaa aaggggtgct gatattgtta accatagctg gggaggttca tggccaagtg aagcgcttcg tgaagcggtg gtgcggacgg cgaacaacgg tctcattcac atcttcgcag caggaaatga cggagtagat atcgaccaga cggcctttta tccagccgca ttctcgactg aagcggacgg gctgatcaca gttgctaatg tgaaaggaga tccagaccat ggagggaaac gtataattga actggatcgt tctagtaact atggaataca gcgcgtccag gtcgcctgtc cagggatgtg gattttatct actgtcccta ccagcggttc ttcgcaagag ccctatgcgg agaagagcgg gacatcaatg gctgctcccg ctctcagcgg catcgttgct ttaatgctcg cggtgaatcc tggactgagt acgcgacagg tcagagaggg tttacgacag tgctccgtcc agcagcccct tttgcaaggc aaagtagaat ggggcagtat gcctgatgct aaacgctgcg tcgagtatgc gctgacaact cacgcagaag gtcggcacaa gtctttcata agggaaccct caaccgagac ttcgacaccc ccacctcctc caccagcaca gcctacaccc cagccgcagc cacatcctcc tcctcaaccc gagactccac catgcgcgcc ttcaccaccg ccgccgaccc caccatgcgc gccttcacca tcgccgccga ccccaccccc ggggtcacca cacaaacctg aaccgcagac gcctgtttac cctgaggttc cacgatccac gcggtcaccg ccgccgtcgc ctcccccaac ggaatctgca cccgaagctc ctccttccga tactccctca tgtcgtgtgc ctccttgcag cagttctcca cgaagcggct ctcaaccaaa gccacctcaa gataacacta ctacaccgaa gatgccctca ctctcctctc caccgaccga acattctaca gcccagccac ctaaacacga aaatgacgca cgtgaacagg agccaccaac cgacgaggat gacttctcat ccgttaaggg taaaaagtta ggtgcctacg aatcggacgg ttcgcctcgg gcctcctcct gtgccggagc aggagttctc ggtgtttttt tcatggttgt tggactgacc gtgtaa

Details for TogoA.18104.a.A2.GE33576

HARVESTED ON:	10/31/2011
SEQUENCED ON:	11/4/2011
EXPECTED MW:	56kDa
OBSERVED MW:	65kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Validated AA Sequence

MKEIRRVEAE IEDEVETLNR DRRLHRGHNK YADDDIRQGL KGEQDMGASE NIPVAELEPQ DLDREAKYPV RMLIVDKRSD DDDEETKTSF VETALHSDLA QRVVKELNGH VDVLRESGVV LVDLPAQTTD KQLQELIETA RAQGTIXEPD HLVQSVNTSS KGSNDPLLDR LWGMDALNVK EAWDIITTGE PNMGGRXPLV CVLDTGIDYN HPDLRDNMEV NQAERDGTLG VDDDNNGEVD DIYGANMLSK ENDPADDHSH GTHVAGTIGA HGNNGIGVAG VAWAPRLLPC KFLAYTGRGY SSDAVRCIDX CVKRGADIVN HSWGGSWPSE ALREAVVRTA NNGLIHIFAA GNDGVDIDQT AFYPAAFSTE ADGLITVANV KGDPDHGGKR IIELDRSSNY GIQRVQVACP GMWILSTVPT SGSSQEPYAE KSGTSMAAPA LSGIVALMLA VNPGLSTRQV REGLRQCSVQ QPLLQGKVEW GSMPDAKRCV EYALTT

Validated NT Sequence

aagttgtcag cgcatactcg acgcagcgtt tagcatcagg catactgccc cattctactt tgccttgcaa aaggggctgc tggacggagc actgtcgtaa accctctctg acctgtcgcg tactcagtcc aggattcacc gcgagcatta aagcaacgat gccgctgaga gcgggagcag ccattgatgt cccgctcttc tccgcatagg gctcttgcga agaaccgctg gtagggacag tagataaaat ccacatccct ggacaggcga cctggacgcg ctgtattcca tagttactag aacgatccag ttcaattata cgtttccctc catggtctgg atctcctttc acattagcaa ctgtgatcag cccgtccgct tcagtcgaga atgcggctgg ataaaaggcc gtctggtcga tatctactcc gtcatttcct gctgcgaaga tgtgaatgag accgttgttc gccgtccgca ccaccgcttc acgaagcgct tcacttggcc atgaacctcc ccagctatgg ttaacaatat cagcacccct tttgacgcan tagtcgatgc agcggactgc atccgagctg tatcctcttc ctgtataggc gaggaatttg cacggaagca gacggggcgc ccatgcaaca ccagcaactc ctataccgtt gtttccgtga gcaccgatgg ttccggcaac atgagtgccg tgagagtgat catccgcagg gtcgttttcc ttgctgagca tgttggcccc gtagatgtcg tcaacctcac cgttattgtc gtcgtcaact ccaagcgtgc cgtccctctc tgcctggttc acttccatgt tgtcccgtaa gtctggatga ttataatcga tgccagtatc taacacacaa actagtggcn tccgaccccc catattaggc tcgccggtag tgatgatgtc ccaagcctct ttcacgttca acgcatccat tccccagaga cgatccagaa gtgggtcatt tgatcctttc gacgatgtat tgacactttg aaccaagtga tccggctcta ntatggtgcc ttgtgccctg gcggtttcga tcaattcctg cagttgtttg tcggtggttt gtgctggaag atccactaaa accacgccac tttctctaag gacgtccaca tgcccgttca gctcctttac aacgcgttgc gcaagatccg aatgtagcgc cgtctccaca aacgaggttt tggtctcttc atcatcatca tcagaacgct tgtccacaat caacatccgc acaggatact tcgcctcccg atctaaatcc tgtggttcga gctccgcaac gggaatattt tctgaagcgc ccatatcttg ctcgcccttt agcccctgtc ggatgtcgtc atcggcatat ttgttgtgcc cacgatgcag ccttctgtct ctgtttagtg tttcaacctc atcttcgatt tccgcttcca ccctccgaat ctccttcatc gaaccaggac cctgggtctg agcttccagg gtac

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKEIRRVEA EIEDEVETLN RDRRLHRGHN KYADDDIRQG LKGEQDMGAS ENIPVAELEP QDLDREAKYP VRMLIVDKRS DDDDEETKTS FVETALHSDL AQRVVKELNG HVDVLRESGV VLVDLPAQTT DKQLQELIET ARAQGTIVEP DHLVQSVNTS SKGSNDPLLD RLWGMDALNV KEAWDIITTG EPNMGGRRPL VCVLDTGIDY NHPDLRDNME VNQAERDGTL GVDDDNNGEV DDIYGANMLS KENDPADDHS HGTHVAGTIG AHGNNGIGVA GVAWAPRLLP CKFLAYTGRG YSSDAVRCID YCVKRGADIV NHSWGGSWPS EALREAVVRT ANNGLIHIFA AGNDGVDIDQ TAFYPAAFST EADGLITVAN VKGDPDHGGK RIIELDRSSN YGIQRVQVAC PGMWILSTVP TSGSSQEPYA EKSGTSMAAP ALSGIVALML AVNPGLSTRQ VREGLRQCSV QQPLLQGKVE WGSMPDAKRC VEYALTT

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaggagatt cggagggtgg aagcggaaat cgaagatgag gttgaaacac taaacagaga cagaaggctg catcgtgggc acaacaaata tgccgatgac gacatccgac aggggctaaa gggcgagcaa gatatgggcg cttcagaaaa tattcccgtt gcggagctcg aaccacagga tttagatcgg gaggcgaagt atcctgtgcg gatgttgatt gtggacaagc gttctgatga tgatgatgaa gagaccaaaa cctcgtttgt ggagacggcg ctacattcgg atcttgcgca acgcgttgta aaggagctga acgggcatgt ggacgtcctt agagaaagtg gcgtggtttt agtggatctt ccagcacaaa ccaccgacaa acaactgcag gaattgatcg aaaccgccag ggcacaaggc accatagtag agccggatca cttggttcaa agtgtcaata catcgtcgaa aggatcaaat gacccacttc tggatcgtct ctggggaatg gatgcgttga acgtgaaaga ggcttgggac atcatcacta ccggcgagcc taatatgggg ggtcggaggc cactagtttg tgtgttagat actggcatcg attataatca tccagactta cgggacaaca tggaagtgaa ccaggcagag agggacggca cgcttggagt tgacgacgac aataacggtg aggttgacga catctacggg gccaacatgc tcagcaagga aaacgaccct gcggatgatc actctcacgg cactcatgtt gccggaacca tcggtgctca cggaaacaac ggtataggag ttgctggtgt tgcatgggcg ccccgtctgc ttccgtgcaa attcctcgcc tatacaggaa gaggatacag ctcggatgca gtccgctgca tcgactactg cgtcaaaagg ggtgctgata ttgttaacca tagctgggga ggttcatggc caagtgaagc gcttcgtgaa gcggtggtgc ggacggcgaa caacggtctc attcacatct tcgcagcagg aaatgacgga gtagatatcg accagacggc cttttatcca gccgcattct cgactgaagc ggacgggctg atcacagttg ctaatgtgaa aggagatcca gaccatggag ggaaacgtat aattgaactg gatcgttcta gtaactatgg aatacagcgc gtccaggtcg cctgtccagg gatgtggatt ttatctactg tccctaccag cggttcttcg caagagccct atgcggagaa gagcgggaca tcaatggctg ctcccgctct cagcggcatc gttgctttaa tgctcgcggt gaatcctgga ctgagtacgc gacaggtcag agagggttta cgacagtgct ccgtccagca gccccttttg caaggcaaag tagaatgggg cagtatgcct gatgctaaac gctgcgtcga gtatgcgctg acaacttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for TogoA.18104.a.A3.GE33577

HARVESTED ON:	10/31/2011
SEQUENCED ON:	11/4/2011
EXPECTED MW:	24kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMHAEGRHKS FIREPSTETS TPPPPPPAQP TPQPQPHPPP QPETPPCAPS PPPPTPPCAP SPSPPTPPPG SPHKPEPQTP VYPEVPRSTR SPPPSPPPTE SAPEAPPSDT PSCRVPPCSS SPRSGSQPKP PQDNTTTPKM PSLSSPPTEH STAQPPKHEN DAREQEPPTD EDDFSSVKGK KLGAYESDGS PRASSCAGAG VLGVFFMVVG LTV

Validated NT Sequence

tttgttnnnt ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgcacgca gaaggtcggc acaagtcttt cataagggaa ccctcaaccg agacttcgac acccccacct cctccaccag cacagcctac accccagccg cagccacatc ctcctcctca acccgagact ccaccatgcg cgccttcacc accgccgccg accccaccat gcgcgccttc accatcgccg ccgaccccac ccccggggtc accacacaaa cctgaaccgc agacgcctgt ttaccctgag gttccacgat ccacgcggtc accgccgccg tcgcctcccc caacggaatc tgcacccgaa gctcctcctt ccgatactcc ctcatgtcgt gtgcctcctt gcagcagttc tccacgaagc ggctctcaac caaagccacc tcaagataac actactacac cgaagatgcc ctcactctcc tctccaccga ccgaacattc tacagcccag ccacctaaac acgaaaatga cgcacgtgaa caggagccac caaccgacga ggatgacttc tcatccgtta agggtaaaaa gttaggtgcc tacgaatcgg acggttcgcc tcgggcctcc tcctgtgccg gagcaggagt tctcggtgtt tttttcatgg ttgttggact gaccgtgtaa taaacagcac gaacaagttc tgcagccaag cttctcgagg ntccngctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gntaannnnn nnnnnnnnnn nnnnnggnnn nnnanaaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcacgcagaa ggtcggcaca agtctttcat aagggaaccc tcaaccgaga cttcgacacc cccacctcct ccaccagcac agcctacacc ccagccgcag ccacatcctc ctcctcaacc cgagactcca ccatgcgcgc cttcaccacc gccgccgacc ccaccatgcg cgccttcacc atcgccgccg accccacccc cggggtcacc acacaaacct gaaccgcaga cgcctgttta ccctgaggtt ccacgatcca cgcggtcacc gccgccgtcg cctcccccaa cggaatctgc acccgaagct cctccttccg atactccctc atgtcgtgtg cctccttgca gcagttctcc acgaagcggc tctcaaccaa agccacctca agataacact actacaccga agatgccctc actctcctct ccaccgaccg aacattctac agcccagcca cctaaacacg aaaatgacgc acgtgaacag gagccaccaa ccgacgagga tgacttctca tccgttaagg gtaaaaagtt aggtgcctac gaatcggacg gttcgcctcg ggcctcctcc tgtgccggag caggagttct cggtgttttt ttcatggttg ttggactgac cgtgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for TogoA.18104.a.A2.PS01474

PURIFICATION DATe:	4/4/2012
CONCENTRATION:	8.2mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAGGAGATT CGGAGGGTGG AAGCGGAAAT CGAAGATGAG GTTGAAACAC TAAACAGAGA CAGAAGGCTG CATCGTGGGC ACAACAAATA TGCCGATGAC GACATCCGAC AGGGGCTAAA GGGCGAGCAA GATATGGGCG CTTCAGAAAA TATTCCCGTT GCGGAGCTCG AACCACAGGA TTTAGATCGG GAGGCGAAGT ATCCTGTGCG GATGTTGATT GTGGACAAGC GTTCTGATGA TGATGATGAA GAGACCAAAA CCTCGTTTGT GGAGACGGCG CTACATTCGG ATCTTGCGCA ACGCGTTGTA AAGGAGCTGA ACGGGCATGT GGACGTCCTT AGAGAAAGTG GCGTGGTTTT AGTGGATCTT CCAGCACAAA CCACCGACAA ACAACTGCAG GAATTGATCG AAACCGCCAG GGCACAAGGC ACCATAGTAG AGCCGGATCA CTTGGTTCAA AGTGTCAATA CATCGTCGAA AGGATCAAAT GACCCACTTC TGGATCGTCT CTGGGGAATG GATGCGTTGA ACGTGAAAGA GGCTTGGGAC ATCATCACTA CCGGCGAGCC TAATATGGGG GGTCGGAGGC CACTAGTTTG TGTGTTAGAT ACTGGCATCG ATTATAATCA TCCAGACTTA CGGGACAACA TGGAAGTGAA CCAGGCAGAG AGGGACGGCA CGCTTGGAGT TGACGACGAC AATAACGGTG AGGTTGACGA CATCTACGGG GCCAACATGC TCAGCAAGGA AAACGACCCT GCGGATGATC ACTCTCACGG CACTCATGTT GCCGGAACCA TCGGTGCTCA CGGAAACAAC GGTATAGGAG TTGCTGGTGT TGCATGGGCG CCCCGTCTGC TTCCGTGCAA ATTCCTCGCC TATACAGGAA GAGGATACAG CTCGGATGCA GTCCGCTGCA TCGACTACTG CGTCAAAAGG GGTGCTGATA TTGTTAACCA TAGCTGGGGA GGTTCATGGC CAAGTGAAGC GCTTCGTGAA GCGGTGGTGC GGACGGCGAA CAACGGTCTC ATTCACATCT TCGCAGCAGG AAATGACGGA GTAGATATCG ACCAGACGGC CTTTTATCCA GCCGCATTCT CGACTGAAGC GGACGGGCTG ATCACAGTTG CTAATGTGAA AGGAGATCCA GACCATGGAG GGAAACGTAT AATTGAACTG GATCGTTCTA GTAACTATGG AATACAGCGC GTCCAGGTCG CCTGTCCAGG GATGTGGATT TTATCTACTG TCCCTACCAG CGGTTCTTCG CAAGAGCCCT ATGCGGAGAA GAGCGGGACA TCAATGGCTG CTCCCGCTCT CAGCGGCATC GTTGCTTTAA TGCTCGCGGT GAATCCTGGA CTGAGTACGC GACAGGTCAG AGAGGGTTTA CGACAGTGCT CCGTCCAGCA GCCCCTTTTG CAAGGCAAAG TAGAATGGGG CAGTATGCCT GATGCTAAAC GCTGCGTCGA GTATGCGCTG ACAACTTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA