TogoA.18099.a

Trypsin, putative

CENTER ID: TogoA.18099.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.18099.a.A3.GE33571 domain 2 617 960
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_290840
RefSeq: XP_002368468.1
UniProt: B6KKU5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVGGVCSPFA VADFSSLLAL KSPFFAALGL SRLFCMQPVE THSHPTENSS AQTRTASLFS LLFFLVVLFL VRSLVCPRFF FSVAFLSSRF NATTVLVAPL FPFLASLAHP APSFGIASSP SLLSSVALSL HPSPLASSPR SSPLLPSHPS PPSSRLRLPA SSSLSSAFRR SPRGCPSAVR LCRLYAHREG SSRGSPVSAS GASQPRSLAC TMASAVLLRR LGFVGSPSAS VASLQGDLAA RHTTQRLLSL AFARRVNSPR SPNQAKRLFS SSSLFSPSSS SSLFSSTLAS RCRSACQSSS PPSFLADRRR LSSSLSASLS SLSSLSPALR PSNGGDPASP RRSSPASLSR SAAFSSASFP VPSQLEGDVL SEPPSGVCTP DGVLGEGAFA QHAAPGVADA LFHGEMPSPA SLSFLFNSVV KVYSDFTDPN YSLPWQMQRQ GTSTGSGFVL RDRLIMTNAH CVSWNNRLQV RKHGSPNKFV ARIVAVGHEC DLALITVDDE AFWQGDLAQL EFGDVPALQD AVVVLGYPRG GDNLCITSGV VSRVDVNPYA HSNTCLLCVQ IDAAINPGNS GGPALKDGRV VGVAFQGFDN AQNIGYIVPT TVIKHFLDDV KRHKGVYTGF PSAGIVFQHL ENKSMQAFLG LDKIQPRQLP PGVEASGILV TMADELRARQ FARHLEGGAQ SAGKREDGER GDSTETNQAG KKKEEPASDS DEDVRLKDGT RVGLKKNDVI LAIDGVDVAN DGTVFFREME RVNVSHTISS KFIGDTLRAT VLRKKEVVDV LVPLIEENAL VPKHQWDKKA RYLIYGGLVF CPLTLEYLKD EFGTKFSERA PASLLQPLAD IFAKEEGEEP VILSHILASD LTSGYTFRNC LLTHVDGQKV LNMKHLASLL GLPLPASSPS SAPSSVSSSP AESSEKKEND FVIFLLENKV QLVLERSKAE SMQPFILKQH AIHSPTSEVL
NT Sequence
atggtcggcg gcgtctgttc tccttttgcg gtcgcagatt tctcttcatt gttggctctc aagtctcctt tcttcgccgc tcttggtctc tcccgcctct tctgcatgca acctgtcgag acgcattctc accctacaga aaacagctct gctcagactc gaactgcctc gctcttctct ctcctctttt tcctcgtcgt tctctttctc gtccggagcc tcgtctgccc gcgcttcttc ttttctgttg ccttcctctc gtctcgattt aacgcgacaa ctgtgctggt ggctcctctc tttcctttcc tcgcctctct tgctcacccg gcgccgagct tcggaatcgc ctcgtcgccg tctctcctct cctcagttgc cctctctctc catccctcgc ctctcgcgtc ttctccacgg agctctccac ttcttccttc tcacccttcg ccgccttcct ctcgtctccg tcttccagcg tcttcttctc tctcttctgc ttttcgacgg tctccgcgtg gttgcccgtc ggctgtacgc ttgtgtcggc tgtatgcaca ccgcgaaggc tcttctcgcg gttcgcctgt gtcggcgtct ggcgcgtctc agcctcggag tcttgcatgc accatggcgt cggcggtgtt gctgagacgc ctcggcttcg ttggaagtcc ttctgcgtct gtcgcttcgc ttcagggaga cctggcagct cgccacacga cgcagaggct tctgtcgctc gcctttgctc ggagggtgaa ctccccccga tccccgaacc aggcgaaaag gctcttctct tcatcgtctc tcttctctcc ttcttcttct tcttctctct tctcttcaac tctggcttct cgctgccgca gcgcctgcca gtcgtcttcg cctcccagct tcctcgccga tcgccggcgc ctctcttctt cgctttctgc gtctctctcc tctctttctt ctctctctcc cgctctgcgg ccttcgaacg ggggcgaccc agcgtctccg cgacggtcct ctcctgcttc tctgtcgcgt tctgccgcct tctcctcggc gtccttcccc gttccgagcc agctcgaggg ggatgtgctc tcggagccgc cctcgggtgt ctgtacaccc gacggtgtgt tgggcgaagg cgccttcgcc caacatgcag cgccaggcgt cgccgacgcg cttttccacg gcgagatgcc ttcgcctgcg tcgctgagtt tcctcttcaa cagcgttgtc aaagtctaca gcgacttcac agacccaaat tactcgcttc cttggcaaat gcagcgacaa ggcacgagca cgggaagcgg tttcgttctc agagatcggc tcatcatgac caatgcgcac tgcgtctcct ggaacaatcg cctgcaagtt cgcaaacacg ggtcgccgaa caagttcgtc gcgcgaattg tggcggtggg acatgagtgc gacctcgctc tcatcacagt cgacgacgaa gccttttggc agggcgactt ggcgcaactt gaattcggcg atgtccccgc tctccaagat gctgtagtgg ttctcggata tcctcgggga ggagacaatc tttgcatcac ctctggcgtc gtcagccggg tcgatgtgaa tccctacgcc cactcaaaca cttgtctcct gtgcgtgcag atcgacgcgg ccattaatcc tggcaacagt ggaggtcctg cgttgaagga cggccgagtt gtcggcgtcg ccttccaagg cttcgacaac gcgcaaaaca taggttacat cgtccccacc acagtcatca agcattttct cgacgacgtg aagagacaca agggcgtcta cacggggttc ccctccgccg gcatcgtttt tcaacatctc gaaaacaaga gcatgcaagc cttcctcggt ctcgacaaaa tccaaccgcg acaactgccg ccaggcgtcg aggcttccgg cattttagtg acgatggcag acgagctgcg cgcgcgtcaa ttcgcgcggc atctcgaggg cggagcgcaa agcgcaggaa agagagaaga cggcgagcga ggcgactcga cagagacgaa ccaagcgggg aagaagaagg aggaacctgc gagcgacagc gacgaagacg ttcgcctgaa agatggaacg cgagttggcc tgaaaaagaa cgacgtcatt ctggcgatag acggagtcga tgtggctaac gatggaactg tcttcttcag agagatggaa agagtaaatg tctcgcatac gatttcttcg aaatttatcg gcgacactct ccgggccacc gtcctgagaa agaaggaggt ggttgacgtc cttgttccgc ttatcgagga gaacgcgctt gttccgaagc accagtggga caagaaggcg cggtacctca tctacggagg acttgtcttc tgtcctctca ctctcgagta cttgaaggat gaatttggaa cgaaattcag cgaacgcgca cctgcctccc ttctccagcc tctcgccgac atctttgcga aggaagaagg agaggaacct gttattctca gccacattct tgcgtcggac ctcacgagcg gatacacgtt ccggaattgc ctcctcactc atgtcgacgg ccagaaagtc ctcaacatga aacatctcgc cagcctcctt ggccttcctc ttcctgcttc ttctccctct tctgcgcctt cttcggtttc ttcttctcct gctgagtcgt cggagaagaa ggagaacgac tttgtcattt ttctgctgga gaacaaagtt caactggttt tggagcggtc gaaagctgag agcatgcagc cgtttatttt gaagcagcac gcgatccact cccccaccag cgaggtcttg tga
Details for TogoA.18099.a.A3.GE33571
HARVESTED ON: 10/31/2011
SEQUENCED ON: 11/7/2011
EXPECTED MW: 40kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMYTGFPSAG IVFQHLENKS MQAFLGLDKI QPRQLPPGVE ASGILVTMAD ELRARQFARH LEGGAQSAGK REDGERGDST ETNQAGKKKE EPASDSDEDV RLKDGTRVGL KKNDVILAID GVDVANDGTV FFREMERVNV SHTISSKFIG DTLRATVLRK KEVVDVLVPL IEENALVPKH QWDKKARYLI YXGXVFCPLT LEYLKXEFGT KFSERAPASL LQPLADXFAX EEGEEPXILS HILASXLTSG YXFRXXLLTH V
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtaca cggggttccc ctccgccggc atcgtttttc aacatctcga aaacaagagc atgcaagcct tcctcggtct cgacaaaatc caaccgcgac aactgccgcc aggcgtcgag gcttccggca ttttagtgac gatggcagac gagctgcgcg cgcgtcaatt cgcgcggcat ctcgagggcg gagcgcaaag cgcaggaaag agagaagacg gcgagcgagg cgactcgaca gagacgaacc aagcggggaa gaagaaggag gaacctgcga gcgacagcga cgaagacgtt cgcctgaaag atggaacgcg agttggcctg aaaaagaacg acgtcattct ggcgatagac ggagtcgatg tggctaacga tggaactgtc ttcttcagag agatggaaag agtaaatgtc tcgcatacga tttcttcgaa atttatcggc gacactctcc gggccaccgt cctgagaaag aaggaggtgg ttgacgtcct tgttccgctt atcgaggaga acgcgcttgt tccgaagcac cagtgggaca agaaggcgcg gtacctcatc tacngaggan ttgtcttctg tcctctcact ctcgagtact tgaagganga atttggaacg aaattcagcg aacgcgcacc tgcctccctt ctccagcctc tcgccgacnt ctttgcgaan gaagaaggag aggaacctgn tattctcagc cacattcttg cgtcnganct cacgagcgga tacncgttcc ggaanngnct cctcactcat gtcga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMYTGFPSAG IVFQHLENKS MQAFLGLDKI QPRQLPPGVE ASGILVTMAD ELRARQFARH LEGGAQSAGK REDGERGDST ETNQAGKKKE EPASDSDEDV RLKDGTRVGL KKNDVILAID GVDVANDGTV FFREMERVNV SHTISSKFIG DTLRATVLRK KEVVDVLVPL IEENALVPKH QWDKKARYLI YGGLVFCPLT LEYLKDEFGT KFSERAPASL LQPLADIFAK EEGEEPVILS HILASDLTSG YTFRNCLLTH VDGQKVLNMK HLASLLGLPL PASSPSSAPS SVSSSPAESS EKKENDFVIF LLENKVQLVL ERSKAESMQP FILKQHAIHS PTSEVL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtacacgggg ttcccctccg ccggcatcgt ttttcaacat ctcgaaaaca agagcatgca agccttcctc ggtctcgaca aaatccaacc gcgacaactg ccgccaggcg tcgaggcttc cggcatttta gtgacgatgg cagacgagct gcgcgcgcgt caattcgcgc ggcatctcga gggcggagcg caaagcgcag gaaagagaga agacggcgag cgaggcgact cgacagagac gaaccaagcg gggaagaaga aggaggaacc tgcgagcgac agcgacgaag acgttcgcct gaaagatgga acgcgagttg gcctgaaaaa gaacgacgtc attctggcga tagacggagt cgatgtggct aacgatggaa ctgtcttctt cagagagatg gaaagagtaa atgtctcgca tacgatttct tcgaaattta tcggcgacac tctccgggcc accgtcctga gaaagaagga ggtggttgac gtccttgttc cgcttatcga ggagaacgcg cttgttccga agcaccagtg ggacaagaag gcgcggtacc tcatctacgg aggacttgtc ttctgtcctc tcactctcga gtacttgaag gatgaatttg gaacgaaatt cagcgaacgc gcacctgcct cccttctcca gcctctcgcc gacatctttg cgaaggaaga aggagaggaa cctgttattc tcagccacat tcttgcgtcg gacctcacga gcggatacac gttccggaat tgcctcctca ctcatgtcga cggccagaaa gtcctcaaca tgaaacatct cgccagcctc cttggccttc ctcttcctgc ttcttctccc tcttctgcgc cttcttcggt ttcttcttct cctgctgagt cgtcggagaa gaaggagaac gactttgtca tttttctgct ggagaacaaa gttcaactgg ttttggagcg gtcgaaagct gagagcatgc agccgtttat tttgaagcag cacgcgatcc actcccccac cagcgaggtc ttgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa