TogoA.18098.a

Subtilase family serine protease, putative (EC 3.4.21.66)

CENTER ID: TogoA.18098.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.18098.a.A2.GE33568 domain 1 33 644
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_200350
RefSeq: XP_002371957.1
UniProt: B6KVT4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEYVERRQKG GFRRWATCGF LCIGVFIVVT AGSKASITSD ETEPQKPEAT LERSGTLLSD LALTTAPTVS QRNGRREGDS HTPGPTDSPE IMRKHSKELD PDEAPSPKAV ASEILFLRRS DTPSRRVVLS YSLKGCPSEK LEVGRAAGHS ASLQGQEASS RTMNLLRDQV SRLLHAKKLV KKPESVSPRG EEILGYGQDY HVSLRVTVEM ESRYLSALQM EVIQTLPEGV TDDEIIQIAK GLPCVEKAYH DSLKFSEQYL RHPVSDGDPD PMNTGTKRRP PQLSKGHSAH YSLDSLGERV TNAFETGDIL SNVDADVQVP PNDPSFRLQW NLQDKAEFGL HTERAWQLWT GQRRPMVIAV IDSGCDLDHP DLNAKKWHNP GEICNDGIDN DGNGFTDDCH GWDFATDRPD VRSDSTGHGT GAAGVLGAVP NNLIGLAGVC WGCEIMCLKF IGGGQGTVSN QVQAIDYAVK MGAWISNNSY GGYGHSNLEF DAIRRAQTAG HLFITSAGNH NLNTDLPQND HTPSSYDLSN ILSVGASTHI GRKAAFSNYG LATVHVFAPG ADIHTTEGEA GYSKVSGTSF ACPHAAGLAG LIWSAFPNLT YLQVKRALIE GCRPSSHLLS LATCGGTLDA NKSLYIAAKL STGSMPDGST SRTHDYVKSG VDLVGPAALL SGLVRGTSPS LAASPSVLSV RASSRESSIN AAIAPPQAHN GGNPTKMPKT DSLQREVNPP FVAPDYRRTI VLGSRSSELD SSSFHSSVAN EGSNIFSDGM GPSSTRPPFE AEEEMSTRQP SATSGEVNAF HEETFGTSRV EAIRVADEDS GTEEAPRSFS LFSGQFLPAA RTAASFPSFD DFFSAPWSVI PTGEEPQLSA HSESSRMSEK NMSSSNVYLT VKMQESVHDR TTATPTGQAV MNIDEQGSST TSRRVARTNK SLPALLKDSF SLSLGLGKEL VTFGNLMAKV PVGNI
NT Sequence
atggaatatg tggaaaggcg tcaaaaggga ggattccgca gatgggcaac atgtggattc ttgtgcatcg gtgtctttat cgtcgtcacg gcgggtagta aggctagcat aacctcagac gaaacggaac cacaaaagcc ggaagcaact ctcgagcgga gtggaactct tctctctgat cttgcgttga caacagcccc caccgtcagt caacgaaatg gaaggcggga gggcgactcc cacacaccgg gtccgacaga ttcaccagag attatgcgga agcacagtaa agagttagat cccgatgaag ctcccagtcc caaagcagtt gcttcagaaa ttctcttcct tcgtcgatca gacacaccga gcagacgtgt cgtgctgagt tactctctca agggttgtcc atcagagaaa ctcgaagtag gccgagctgc agggcattcg gcttctctcc agggacaaga agctagcagc cgtacgatga atctgttaag agaccaagtt tctcggctcc tgcatgcgaa aaagctggta aaaaaacctg aatccgtgag tccacgaggt gaagaaattt tggggtatgg acaagactat catgtgagtt tgagggtgac ggtcgagatg gaaagccgat atttgtcagc tctgcaaatg gaagtcattc aaacactacc tgagggggtc acggatgacg aaatcataca aattgctaag ggacttcctt gcgtcgagaa ggcataccac gattccttga aattctctga gcaatacttg aggcatcctg tttctgatgg agatccggat cccatgaaca cgggaacaaa gcgccgtcct ccgcagctaa gcaaaggcca ctctgcacac tattctcttg attccctcgg ggaacgcgtt acgaacgctt ttgagacagg agacatttta tcaaatgtgg atgctgacgt tcaagtgcct cctaatgacc caagcttccg cctccagtgg aatctacagg ataaggcaga gttcggtctg cacaccgaaa gagcgtggca gctctggacg ggtcagaggc gacccatggt gattgcagtc attgatagcg gctgtgatct agaccatcca gacctcaacg ccaaaaagtg gcacaaccct ggagaaattt gcaacgacgg tatcgacaac gacggaaacg gttttactga cgactgccat gggtgggact tcgcgacaga tcgtccagac gttcgctcgg actccactgg gcacggaaca ggagctgcag gcgttcttgg cgcggtgccg aacaatttga taggcctggc tggggtgtgc tggggctgtg aaatcatgtg tctgaaattc attggtggtg gtcaaggaac tgttagcaac caagttcaag ccattgacta tgcggttaaa atgggagcct ggattagtaa caattcttac ggtggatacg gacattcgaa tcttgaattt gacgccatcc ggcgagctca aactgctggt catctcttca tcacctccgc agggaatcac aacctgaaca ccgatctacc acagaacgac catactcctt cgagttacga cctctctaac atcctgagtg tgggagcgtc gacgcacatc ggtagaaagg cggccttctc taactatgga ctcgcgactg tccacgtgtt cgctcctggc gctgatattc acacgaccga aggcgaggca gggtactcta aagtcagtgg gacctctttc gcatgcccac atgccgcggg gttggctggg ttgatttggt cggcgttccc caacttgacc taccttcaag tgaagcgagc tttgatcgag ggatgcaggc caagttcgca cctcttgtca ctggccacat gtggagggac tctcgacgct aacaagtctc tctatattgc cgcgaaacta tccactgggt ctatgcctga cgggagcaca tcgcggacac atgactatgt gaagtcaggc gtagatttag taggtccagc cgcccttcta tcggggctgg tgagaggaac atcgccttcc ttagctgcat ccccatcagt tctatccgtc cgtgcgtctt cgcgcgaaag ttcaatcaat gctgcaattg cccctcctca agcccataat ggagggaacc caacaaagat gcccaaaaca gattctctcc agagggaggt gaatccaccg tttgtcgccc ccgattatcg acgcacgatt gtgctggggt cacgctcatc ggaactggat tcttcttcgt ttcactcatc tgtagcaaac gaaggaagta atattttttc tgatggaatg ggaccatctt caaccagacc gcctttcgag gcggaagaag agatgtccac tcgacagcca tctgccacat caggggaagt gaatgcgttt catgaagaga cgtttggtac gtccagagtt gaagcgatca gagtcgctga tgaagattca gggacagaag aggccccgcg ttccttctca ctgtttagtg ggcaattcct tcccgcggcg agaacagctg cgtcgtttcc ctcgttcgat gacttttttt ctgctccctg gagtgtgata ccaaccggag aagaaccgca actttccgct cattcggaga gctcaaggat gtccgaaaaa aatatgtctt cttcgaatgt atatttgaca gtgaagatgc aggagagtgt tcatgatcgc acaacggcga cgccgacagg gcaagccgtg atgaacatag atgagcaggg atcatccact acaagtcggc gagtagccag gacaaacaaa agcttacccg ccttgctgaa agatagcttt tctctgtcgt taggacttgg aaaagagctt gtaacatttg ggaacctgat ggccaaggta ccggtcggca acatctga
Details for TogoA.18098.a.A2.GE33568
HARVESTED ON: data unavailable
SEQUENCED ON: 10/2/2013
EXPECTED MW: 68kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 50
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKASITSD ETEPQKPEAT LERSGTLLSD LALTTAPTVS QRNGRREGDS HTPGPTDSPE IMRKHSKELD PDEAPSPKAV ASEILFLRRS DTPSRRVVLS YSLKGCPSEK LEVGRAAGHS ASLQGQEASS RTMNLLRDQV SRLLHAKKLV KKPESVSPRG EEILGYGQDY HVSLRVTVEM ESRYLSALQM EVIQTLPEGV TDDEIIQIAK GLPCVEKAYH DSLKFSEQYL RHPVSDGDPD PMNTGTKRRP PQLXKGHSAH YSLDSLGERV TNAFETGDIL SNVDADVQVP XNDPXXRL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagta aggctagcat aacctcagac gaaacggaac cacaaaagcc ggaagcaact ctcgagcgga gtggaactct tctctctgat cttgcgttga caacagcccc caccgtcagt caacgaaatg gaaggcggga gggcgactcc cacacaccgg gtccgacaga ttcaccagag attatgcgga agcacagtaa agagttagat cccgatgaag ctcccagtcc caaagcagtt gcttcagaaa ttctcttcct tcgtcgatca gacacaccga gcagacgtgt cgtgctgagt tactctctca agggttgtcc atcagagaaa ctcgaagtag gccgagctgc agggcattcg gcttctctcc agggacaaga agctagcagc cgtacgatga atctgttaag agaccaagtt tctcggctcc tgcatgcgaa aaagctggta aaaaaacctg aatccgtgag tccacgaggt gaagaaattt tggggtatgg acaagactat catgtgagtt tgagggtgac ggtcgagatg gaaagccgat atttgtcagc tctgcaaatg gaagtcattc aaacactacc tgagggggtc acggatgacg aaatcataca aattgctaag ggacttcctt gcgtcgagaa ggcataccac gattccttga aattctctga gcaatacttg aggcatcctg tttctgatgg agatccggat cccatgaaca cgggaacaaa gcgccgtcct ccgcagctaa ncaaaggcca ctctgcacac tattctcttg attccctcgg ggaacgcgtt acgaacgctt ttgagacagg agacatttta tcaaatgtgg atgctgacgt tcaagtgccn nctaatgacc cnngcttncg cctcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKASITSD ETEPQKPEAT LERSGTLLSD LALTTAPTVS QRNGRREGDS HTPGPTDSPE IMRKHSKELD PDEAPSPKAV ASEILFLRRS DTPSRRVVLS YSLKGCPSEK LEVGRAAGHS ASLQGQEASS RTMNLLRDQV SRLLHAKKLV KKPESVSPRG EEILGYGQDY HVSLRVTVEM ESRYLSALQM EVIQTLPEGV TDDEIIQIAK GLPCVEKAYH DSLKFSEQYL RHPVSDGDPD PMNTGTKRRP PQLSKGHSAH YSLDSLGERV TNAFETGDIL SNVDADVQVP PNDPSFRLQW NLQDKAEFGL HTERAWQLWT GQRRPMVIAV IDSGCDLDHP DLNAKKWHNP GEICNDGIDN DGNGFTDDCH GWDFATDRPD VRSDSTGHGT GAAGVLGAVP NNLIGLAGVC WGCEIMCLKF IGGGQGTVSN QVQAIDYAVK MGAWISNNSY GGYGHSNLEF DAIRRAQTAG HLFITSAGNH NLNTDLPQND HTPSSYDLSN ILSVGASTHI GRKAAFSNYG LATVHVFAPG ADIHTTEGEA GYSKVSGTSF ACPHAAGLAG LIWSAFPNLT YLQVKRALIE GCRPSSHLLS LATCGGTLDA NKSLYIAAKL STGS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtaaggct agcataacct cagacgaaac ggaaccacaa aagccggaag caactctcga gcggagtgga actcttctct ctgatcttgc gttgacaaca gcccccaccg tcagtcaacg aaatggaagg cgggagggcg actcccacac accgggtccg acagattcac cagagattat gcggaagcac agtaaagagt tagatcccga tgaagctccc agtcccaaag cagttgcttc agaaattctc ttccttcgtc gatcagacac accgagcaga cgtgtcgtgc tgagttactc tctcaagggt tgtccatcag agaaactcga agtaggccga gctgcagggc attcggcttc tctccaggga caagaagcta gcagccgtac gatgaatctg ttaagagacc aagtttctcg gctcctgcat gcgaaaaagc tggtaaaaaa acctgaatcc gtgagtccac gaggtgaaga aattttgggg tatggacaag actatcatgt gagtttgagg gtgacggtcg agatggaaag ccgatatttg tcagctctgc aaatggaagt cattcaaaca ctacctgagg gggtcacgga tgacgaaatc atacaaattg ctaagggact tccttgcgtc gagaaggcat accacgattc cttgaaattc tctgagcaat acttgaggca tcctgtttct gatggagatc cggatcccat gaacacggga acaaagcgcc gtcctccgca gctaagcaaa ggccactctg cacactattc tcttgattcc ctcggggaac gcgttacgaa cgcttttgag acaggagaca ttttatcaaa tgtggatgct gacgttcaag tgcctcctaa tgacccaagc ttccgcctcc agtggaatct acaggataag gcagagttcg gtctgcacac cgaaagagcg tggcagctct ggacgggtca gaggcgaccc atggtgattg cagtcattga tagcggctgt gatctagacc atccagacct caacgccaaa aagtggcaca accctggaga aatttgcaac gacggtatcg acaacgacgg aaacggtttt actgacgact gccatgggtg ggacttcgcg acagatcgtc cagacgttcg ctcggactcc actgggcacg gaacaggagc tgcaggcgtt cttggcgcgg tgccgaacaa tttgataggc ctggctgggg tgtgctgggg ctgtgaaatc atgtgtctga aattcattgg tggtggtcaa ggaactgtta gcaaccaagt tcaagccatt gactatgcgg ttaaaatggg agcctggatt agtaacaatt cttacggtgg atacggacat tcgaatcttg aatttgacgc catccggcga gctcaaactg ctggtcatct cttcatcacc tccgcaggga atcacaacct gaacaccgat ctaccacaga acgaccatac tccttcgagt tacgacctct ctaacatcct gagtgtggga gcgtcgacgc acatcggtag aaaggcggcc ttctctaact atggactcgc gactgtccac gtgttcgctc ctggcgctga tattcacacg accgaaggcg aggcagggta ctctaaagtc agtgggacct ctttcgcatg cccacatgcc gcggggttgg ctgggttgat ttggtcggcg ttccccaact tgacctacct tcaagtgaag cgagctttga tcgagggatg caggccaagt tcgcacctct tgtcactggc cacatgtgga gggactctcg acgctaacaa gtctctctat attgccgcga aactatccac tgggtcttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa