TogoA.18075.a

Zinc carboxypeptidase, putative (EC 3.4.17.2)

CENTER ID: TogoA.18075.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.18075.a.A2.GE33565 domain 1 32 879
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_271870
RefSeq: XP_002365832.1
UniProt: B6KDA9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGDAVPSIA RSRNASAPVS FESFIPRSLS ANATVASGAR PSEASSSAED PRGVVNDPFF LLDHGSMPYG YPTYDEAFRM MNQLLQTYGP SLISEHRIGH SFEGRPIVAF RVGGLLKRRG GASVSPSLSP PVPSPLGGGS AAPGQTREQR SSDESPLSAS GERKQDEEFP EILLTGLHHA REPMSMTMCI YFMARLLRDC QAGEAEAVYL MHLREFWVVP FLNPDAYVAI EKTGNTQLRK NRRRFSSEGR PAHAKLEDEG VDLNRNYAFH FLLAQSEGSD DYGGPFPFSE PETAAVKFLV EQYRRSSPPT PPPPASPSSA SSSELHRMID FSPPQSSSFL EDLGRFEVAL NFHTYGEVWT RPFNCCKEMP LPRWAQRAFE ELQVSLAIPT LNSAPNIAVL GYPTQGEADD WLLHEHNVLS TSPEIGWEEG SFWQTVGKQR ESLEVNFARI VTAAVKAGSE LGVRLSLGWR HFPPSDGEKE MGEETVSKLE GGDDFLREPD GEEERGFRRS LRRRIRERNE RNVEEPTDNT TSTGATSNVL QNAVPSDRLA FVEETLKQKG WKSILELQAN ATVSMHVIEV VNTGLLPLHG SATLLFLTGL PLFSSPLVAT SLSSLFTSVE DAAASAPSPL RSRLEPRFTG SRSKIELPPP EALRTFQAFQ SYQSSLSPSR SPSSPSSRSP SPSPSRSPSP SSGLNDLRWG VLDEERADEP AGEKGEDWYF LAFDEKWKTV FPVSVVSPSS RFVEGDATEE GPELPGLLVE IPSAVRGRDF DSGDAGVVRV HLLLLETKSD AAFPPEFSPR SERRPSQERS ASSRDRRRSQ RPAVGACVLE TGPRELKLSP QSGEASEART VPLFCRCGAV KTEAEEADLS GVEHEGDFKW ISLPFAALLL LTLFLVLGLA LRLRFSSESD AGRSVYPCAS P
NT Sequence
atgacgggtg acgcggtgcc gtcaatagct cgcagtcgca acgcgtcggc gcctgtctcc ttcgagtcct tcattccgcg cagcttgtcc gccaacgcca cagttgcctc tggcgcgcgt cccagcgaag cttcgtcgag cgcagaagat cctcgaggag tggtgaacga tccgttcttc ctcctcgacc atggatctat gccgtatggc tatccaactt acgatgaagc ctttcgcatg atgaatcagt tgcttcagac ctacgggcct tctcttatct ctgagcaccg catcggtcac tcttttgaag gtcgtccgat tgtcgccttc cgtgtcggcg gcttgctgaa gcgccgggga ggtgcctcgg tctcgccgtc tctgtctcct ccggttccct cgcctctggg aggcgggagt gcagctccag ggcaaactcg agagcagcga agcagtgacg agagccctct ctctgcgagt ggcgaaagaa agcaggacga ggagtttcct gagattctcc tcacaggcct ccatcatgcc cgggaaccga tgagcatgac catgtgcatc tacttcatgg ctcggcttct gcgggactgc caagcagggg aggccgaggc cgtgtacttg atgcacttgc gcgagttttg ggttgtgcct ttcctcaatc cggacgcgta tgtggcgatc gagaagactg gaaacacaca gctccgcaaa aaccgccgac gctttagctc cgagggtcga ccggctcatg cgaagctcga agacgaaggc gtcgacctaa acagaaacta tgcgttccac ttccttctcg cgcaatctga aggaagcgac gactacggag gtccgttccc cttcagcgag ccagagacgg cagcagtgaa gttccttgtg gagcagtatc gacgttcgag tccgccaacg ccgcctcctc ccgcgtctcc ctcgtctgcc tcgtcttccg agcttcaccg catgatcgac ttttcgcctc cgcagtcgtc ttcgttcctc gaggacttgg gccgctttga ggttgccctc aacttccaca cttacggcga agtctggacg cgaccgttca actgctgcaa agagatgcct ctgcctcgct gggcgcagag agccttcgag gaactccaag tatctctggc tattccaact ttgaacagtg caccgaacat cgcggtttta gggtacccta ctcaggggga agctgacgac tggctgctac atgaacacaa cgtgctatcg acttcgccgg aaattgggtg ggaagaaggc agcttttggc agaccgtcgg caaacaaaga gagtctctgg aagtcaattt cgcaagaatc gtcacggcgg ctgttaaggc gggaagcgag ctgggtgtgc gcctcagttt ggggtggaga cactttccgc cgtcggacgg ggagaaggag atgggggagg agacagtttc aaagctcgag ggtggcgacg actttcttcg ggagccagac ggagaagaag aacgcggctt caggcgaagc ttaaggcgca ggattcgaga gcgaaatgaa aggaacgtgg aagaacctac cgacaacacc acctcgacgg gggcgacgag caacgtcctc caaaacgctg tgccgagcga tcggctggcg ttcgtggagg agacgctgaa gcagaaaggt tggaagtcga ttctcgagtt acaggcgaac gccacagtct ctatgcacgt catcgaggtg gtgaataccg gcctcctgcc tctccacgga tctgccacac ttctctttct aaccggcctt cccctcttct cttcccctct tgtcgcgact tctctctcct ccctgttcac gagtgtggag gacgcggccg cttccgcgcc gtcccccctg cgctcgcgct tggagcctcg gttcactggc agtcgatcga aaatcgaatt gcctcctccg gaggccctgc gcaccttcca ggctttccag tcttaccagt cttccttgtc tccttctcgc tctccttctt ctccttcttc tcgctcgccg tctccttctc cttctcgctc gccgtctcct tcttctggtt tgaacgatct gcgttggggt gtcttggacg aggagagagc cgacgaaccg gctggagaga agggagagga ttggtatttc ctagcgtttg atgagaagtg gaagacggtt ttccccgttt ctgtggtctc gccctcgagt cgcttcgtcg agggagacgc gacggaggag ggtcctgagc ttccaggatt gctagtcgag atcccctcgg ccgttcgcgg ccgtgacttt gacagcggcg acgctggcgt tgtgcgtgtg catcttcttc tgctcgagac gaagagcgat gctgcctttc ctcccgagtt ttctccgcgt tcggagcgcc ggccatctca ggagagatcc gcctcttccc gagaccgacg tcgctctcag cgtcctgctg tcggtgcatg cgtcctcgag actggaccgc gtgagctcaa gctttcacct caaagcggag aagcgtccga ggcgcgaacc gtgccgttgt tttgccgctg tggggccgtg aagactgaag cagaggaagc cgacctgtct ggagtcgagc acgaaggcga cttcaagtgg atttctctcc cgttcgccgc tttgctgctg ctcacgctct tcctcgtcct cggtttggcc ctgcgcctgc gattcagttc agagtccgat gccggtcggt ctgtgtaccc ctgtgcttcg ccttga
Details for TogoA.18075.a.A2.GE33565
HARVESTED ON: 10/31/2011
SEQUENCED ON: 11/7/2011
EXPECTED MW: 95kDa
OBSERVED MW: 95kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 28
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNATVASGA RPSEASSSAE DPRGVVNDPF FLLDHGSMPY GYPTYDEAFR MMNQLLQTYG PSLISEHRIG HSFEGRPIVA FRVGGLLKRR GGASVSPSLS PPVPSPLGGG SAAPGQTREQ RSSDESPLSA SGERKQDEEF PEILLTGLHH AREPMSMTMC IYFMARLLRD CQAGEAEAVY LMHLREFWVV PFLXPDAYVA IEKTGNTQLR KNXRRFSSXG RPAHAKLED
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaacg ccacagttgc ctctggcgcg cgtcccagcg aagcttcgtc gagcgcagaa gatcctcgag gagtggtgaa cgatccgttc ttcctcctcg accatggatc tatgccgtat ggctatccaa cttacgatga agcctttcgc atgatgaatc agttgcttca gacctacggg ccttctctta tctctgagca ccgcatcggt cactcttttg aaggtcgtcc gattgtcgcc ttccgtgtcg gcggcttgct gaagcgccgg ggaggtgcct cggtctcgcc gtctctgtct cctccggttc cctcgcctct gggaggcggg agtgcagctc cagggcaaac tcgagagcag cgaagcagtg acgagagccc tctctctgcg agtggcgaaa gaaagcagga cgaggagttt cctgagattc tcctcacagg cctccatcat gcccgggaac cgatgagcat gaccatgtgc atctacttca tggctcggct tctgcgggac tgccaagcag gggaggccga ggccgtgtac ttgatgcact tgcgcgagtt ttgggttgtg cctttcctcn atccggacgc gtatgtggcg atcgagaaga ctggnaacac acagctccgc aaaaacngcc gacgctttag ctccnanggt cgaccggctc atgcgaagct cgaagacnaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNATVASGA RPSEASSSAE DPRGVVNDPF FLLDHGSMPY GYPTYDEAFR MMNQLLQTYG PSLISEHRIG HSFEGRPIVA FRVGGLLKRR GGASVSPSLS PPVPSPLGGG SAAPGQTREQ RSSDESPLSA SGERKQDEEF PEILLTGLHH AREPMSMTMC IYFMARLLRD CQAGEAEAVY LMHLREFWVV PFLNPDAYVA IEKTGNTQLR KNRRRFSSEG RPAHAKLEDE GVDLNRNYAF HFLLAQSEGS DDYGGPFPFS EPETAAVKFL VEQYRRSSPP TPPPPASPSS ASSSELHRMI DFSPPQSSSF LEDLGRFEVA LNFHTYGEVW TRPFNCCKEM PLPRWAQRAF EELQVSLAIP TLNSAPNIAV LGYPTQGEAD DWLLHEHNVL STSPEIGWEE GSFWQTVGKQ RESLEVNFAR IVTAAVKAGS ELGVRLSLGW RHFPPSDGEK EMGEETVSKL EGGDDFLREP DGEEERGFRR SLRRRIRERN ERNVEEPTDN TTSTGATSNV LQNAVPSDRL AFVEETLKQK GWKSILELQA NATVSMHVIE VVNTGLLPLH GSATLLFLTG LPLFSSPLVA TSLSSLFTSV EDAAASAPSP LRSRLEPRFT GSRSKIELPP PEALRTFQAF QSYQSSLSPS RSPSSPSSRS PSPSPSRSPS PSSGLNDLRW GVLDEERADE PAGEKGEDWY FLAFDEKWKT VFPVSVVSPS SRFVEGDATE EGPELPGLLV EIPSAVRGRD FDSGDAGVVR VHLLLLETKS DAAFPPEFSP RSERRPSQER SASSRDRRRS QRPAVGACVL ETGPRELKLS PQSGEASEAR TVPLFCRCGA VKTEAEEADL SGVEHEGDFK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgccaca gttgcctctg gcgcgcgtcc cagcgaagct tcgtcgagcg cagaagatcc tcgaggagtg gtgaacgatc cgttcttcct cctcgaccat ggatctatgc cgtatggcta tccaacttac gatgaagcct ttcgcatgat gaatcagttg cttcagacct acgggccttc tcttatctct gagcaccgca tcggtcactc ttttgaaggt cgtccgattg tcgccttccg tgtcggcggc ttgctgaagc gccggggagg tgcctcggtc tcgccgtctc tgtctcctcc ggttccctcg cctctgggag gcgggagtgc agctccaggg caaactcgag agcagcgaag cagtgacgag agccctctct ctgcgagtgg cgaaagaaag caggacgagg agtttcctga gattctcctc acaggcctcc atcatgcccg ggaaccgatg agcatgacca tgtgcatcta cttcatggct cggcttctgc gggactgcca agcaggggag gccgaggccg tgtacttgat gcacttgcgc gagttttggg ttgtgccttt cctcaatccg gacgcgtatg tggcgatcga gaagactgga aacacacagc tccgcaaaaa ccgccgacgc tttagctccg agggtcgacc ggctcatgcg aagctcgaag acgaaggcgt cgacctaaac agaaactatg cgttccactt ccttctcgcg caatctgaag gaagcgacga ctacggaggt ccgttcccct tcagcgagcc agagacggca gcagtgaagt tccttgtgga gcagtatcga cgttcgagtc cgccaacgcc gcctcctccc gcgtctccct cgtctgcctc gtcttccgag cttcaccgca tgatcgactt ttcgcctccg cagtcgtctt cgttcctcga ggacttgggc cgctttgagg ttgccctcaa cttccacact tacggcgaag tctggacgcg accgttcaac tgctgcaaag agatgcctct gcctcgctgg gcgcagagag ccttcgagga actccaagta tctctggcta ttccaacttt gaacagtgca ccgaacatcg cggttttagg gtaccctact cagggggaag ctgacgactg gctgctacat gaacacaacg tgctatcgac ttcgccggaa attgggtggg aagaaggcag cttttggcag accgtcggca aacaaagaga gtctctggaa gtcaatttcg caagaatcgt cacggcggct gttaaggcgg gaagcgagct gggtgtgcgc ctcagtttgg ggtggagaca ctttccgccg tcggacgggg agaaggagat gggggaggag acagtttcaa agctcgaggg tggcgacgac tttcttcggg agccagacgg agaagaagaa cgcggcttca ggcgaagctt aaggcgcagg attcgagagc gaaatgaaag gaacgtggaa gaacctaccg acaacaccac ctcgacgggg gcgacgagca acgtcctcca aaacgctgtg ccgagcgatc ggctggcgtt cgtggaggag acgctgaagc agaaaggttg gaagtcgatt ctcgagttac aggcgaacgc cacagtctct atgcacgtca tcgaggtggt gaataccggc ctcctgcctc tccacggatc tgccacactt ctctttctaa ccggccttcc cctcttctct tcccctcttg tcgcgacttc tctctcctcc ctgttcacga gtgtggagga cgcggccgct tccgcgccgt cccccctgcg ctcgcgcttg gagcctcggt tcactggcag tcgatcgaaa atcgaattgc ctcctccgga ggccctgcgc accttccagg ctttccagtc ttaccagtct tccttgtctc cttctcgctc tccttcttct ccttcttctc gctcgccgtc tccttctcct tctcgctcgc cgtctccttc ttctggtttg aacgatctgc gttggggtgt cttggacgag gagagagccg acgaaccggc tggagagaag ggagaggatt ggtatttcct agcgtttgat gagaagtgga agacggtttt ccccgtttct gtggtctcgc cctcgagtcg cttcgtcgag ggagacgcga cggaggaggg tcctgagctt ccaggattgc tagtcgagat cccctcggcc gttcgcggcc gtgactttga cagcggcgac gctggcgttg tgcgtgtgca tcttcttctg ctcgagacga agagcgatgc tgcctttcct cccgagtttt ctccgcgttc ggagcgccgg ccatctcagg agagatccgc ctcttcccga gaccgacgtc gctctcagcg tcctgctgtc ggtgcatgcg tcctcgagac tggaccgcgt gagctcaagc tttcacctca aagcggagaa gcgtccgagg cgcgaaccgt gccgttgttt tgccgctgtg gggccgtgaa gactgaagca gaggaagccg acctgtctgg agtcgagcac gaaggcgact tcaagtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa