TogoA.18073.a

Putative uncharacterized protein

CENTER ID: TogoA.18073.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.18073.a.A3.GE33583 domain 2 229 547
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_312660
RefSeq: XP_002364488.1
UniProt: B6K8W4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNAYFSRGG EERRQRSGER DRKEGSASRL GDSEQGECYL DSVSLQAAFR DFQSRVPLKK LVSTQNAHHI WQWYELCGTD LDCEASPLIS SSPIIFLHGV NGTAAIYFQQ LEALAEKGYR VLSVQWPALS SLASFLSSFD EFLTHVLATP AASRVPRSRR RLVSSAAARG DGDAGEGRVR RGAHLFGAGL GGVLCLYFAQ RRPEAVASVV LCNAFLNSAF LLGALAPLQG VLAPMYHLLP HAALRKIIID TYLTPKAAAE AVFVPPSGVC TGARGRRDES PENVWAPSQG EADSHSQGVW VVPQEDLQIR NSKEFLVSHL DELSAKELAA RLALQVAISA ATPLQKTPER VLILQSLDAD VPPDVAEDLR NFFPRAKVAE LRDGGQFPFL SRPEEVTLHM QVHLRNCGLF PDLHLIERQV SDSSCFSSER ALSSSYSRSA SSCYNSARNH SSLAGGSSAD NAFYNSSSFD RGSHRGAAGC FVPASSPATS QATASDGAAS SRGISTNSGE RWEEREREKT SKFADADLAA QERWNRKHWK NPFKEAD
NT Sequence
atgacaaacg cgtacttctc gaggggcggc gaggaacgcc gacagaggag tggagagaga gacagaaaag aagggagtgc aagcaggcta ggggactctg aacaaggcga atgctatctc gacagtgtct ccctccaagc cgctttccga gacttccaga gccgtgtccc tctcaagaaa ctcgtcagca cgcaaaacgc acatcacatc tggcagtggt atgagctgtg cggaacggat cttgactgtg aggcctcgcc gttgatttct tcttctccca tcattttcct ccatggcgtg aacgggactg ccgcgattta ctttcagcag ctggaggcac tggcagagaa ggggtacaga gtcttgtcgg tgcagtggcc cgcactgtct tctctcgcat ctttcctgtc ttccttcgac gaatttctga ctcacgtcct ggcgactccg gctgcctcgc gggtgccgcg ctctcgccgc cggcttgtct cctcagccgc ggcgcgcgga gacggcgacg caggcgaggg gcgcgtacgc cgcggcgcgc acttgttcgg cgcaggcctc ggcggcgttc tgtgtctgta ctttgctcag aggcgtccag aagccgtcgc aagcgtcgtg ctctgcaacg ccttcctgaa ttctgcgttc ctcctggggg cgctcgcgcc tctccaaggc gtcctagctc ccatgtacca cctgctgccg catgcagcgc tcagaaaaat catcatcgac acctacctca cccccaaggc tgccgccgag gccgtcttcg tcccgccgtc gggtgtctgt acaggcgcac gcggcaggcg cgacgagtcc ccagagaacg tctgggcgcc gtcgcaggga gaggcagaca gccactcaca gggcgtttgg gtcgttccgc aagaagatct gcaaattcga aacagcaaag agttcctcgt ttctcacctc gacgaactca gcgcgaagga gctcgcggcg aggctcgcct tgcaagtggc gataagcgcc gcgacaccgt tgcagaaaac gcccgaaaga gtcctcattc tgcaatccct cgacgccgac gttcctcccg atgtcgccga ggatctgcgg aacttcttcc cgcgcgcgaa agtcgcggag ctgcgcgacg gcggtcaatt cccctttctg tcgcgccccg aagaagtgac tctgcacatg caagttcact tgcggaactg cggtctcttt ccagatttgc atttgataga gagacaagtt tcagacagca gctgcttctc cagcgaaaga gccttgtctt cctcgtatag cagatctgct tcctcatgtt acaactcggc ccgcaaccac tcttcgttgg ctggcggctc ttctgcagac aacgctttct acaactcttc gtctttcgac cgcgggagtc accgaggcgc tgcaggctgt ttcgtgcctg cgtcttcgcc cgcgacttcg caggcaaccg cgagcgacgg agctgcatcc agtcgaggga tctcgacgaa cagcggcgaa cggtgggagg agcgcgagag agaaaaaaca agcaaatttg ctgatgcaga cctcgcagcg caggaaaggt ggaatcgcaa acactggaaa aatccattca aagaagccga ctag
Details for TogoA.18073.a.A3.GE33583
HARVESTED ON: 10/31/2011
SEQUENCED ON: 11/7/2011
EXPECTED MW: 37kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 79
PERCENT COVERAGE: 86
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQGVLAPMY HLLPHAALRK IIIDTYLTPK AAAEAVFVPP SGVCTGARGR RDESPENVWA PSQGEADSHS QGVWVVPQED LQIRNSKEFL VSHLDELSAK ELAARLALQV AISAATPLQK TPERVLILQS LDADVPPDVA EDLRNFFPRA KVAELRDGGQ FPFLSRPEEV TLHMQVHLRN CGLFPDLHLI ERQVSXEETS CSLANTFAAS GRSSFSLSES HTDHPVSFPX YPHSCSRSPX SPASRSHSSA HGHSSFSSDS SCFSSERXLS XSYS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcaag gcgtcctagc tcccatgtac cacctgctgc cgcatgcagc gctcagaaaa atcatcatcg acacctacct cacccccaag gctgccgccg aggccgtctt cgtcccgccg tcgggtgtct gtacaggcgc acgcggcagg cgcgacgagt ccccagagaa cgtctgggcg ccgtcgcagg gagaggcaga cagccactca cagggcgttt gggtcgttcc gcaagaagat ctgcaaattc gaaacagcaa agagttcctc gtttctcacc tcgacgaact cagcgcgaag gagctcgcgg cgaggctcgc cttgcaagtg gcgataagcg ccgcgacacc gttgcagaaa acgcccgaaa gagtcctcat tctgcaatcc ctcgacgccg acgttcctcc cgatgtcgcc gaggatctgc ggaacttctt cccgcgcgcg aaagtcgcgg agctgcgcga cggcggtcaa ttcccctttc tgtcgcgccc cgaagaagtg actctgcaca tgcaagttca cttgcggaac tgcggtctct ttccagattt gcatttgata gagagacaag tttcangtga agaaacctca tgttcactcg cgaacacctt cgcagcttct ggccgaagct ctttttctct ctctgaaagc cacacggacc atcctgtctc gttcccganc tacccccaca gttgctcgcg ctctcccngc tctcccgcct ctcgctctca ctcttctgcg cacggtcact cttctttttc ctcagacagc agctgcttct ccagcgaaag anccttgtcn tnntcntata gca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQGVLAPMY HLLPHAALRK IIIDTYLTPK AAAEAVFVPP SGVCTGARGR RDESPENVWA PSQGEADSHS QGVWVVPQED LQIRNSKEFL VSHLDELSAK ELAARLALQV AISAATPLQK TPERVLILQS LDADVPPDVA EDLRNFFPRA KVAELRDGGQ FPFLSRPEEV TLHMQVHLRN CGLFPDLHLI ERQVSDSSCF SSERALSSSY SRSASSCYNS ARNHSSLAGG SSADNAFYNS SSFDRGSHRG AAGCFVPASS PATSQATASD GAASSRGIST NSGERWEERE REKTSKFADA DLAAQERWNR KHWKNPFKEA D
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaaggcgtc ctagctccca tgtaccacct gctgccgcat gcagcgctca gaaaaatcat catcgacacc tacctcaccc ccaaggctgc cgccgaggcc gtcttcgtcc cgccgtcggg tgtctgtaca ggcgcacgcg gcaggcgcga cgagtcccca gagaacgtct gggcgccgtc gcagggagag gcagacagcc actcacaggg cgtttgggtc gttccgcaag aagatctgca aattcgaaac agcaaagagt tcctcgtttc tcacctcgac gaactcagcg cgaaggagct cgcggcgagg ctcgccttgc aagtggcgat aagcgccgcg acaccgttgc agaaaacgcc cgaaagagtc ctcattctgc aatccctcga cgccgacgtt cctcccgatg tcgccgagga tctgcggaac ttcttcccgc gcgcgaaagt cgcggagctg cgcgacggcg gtcaattccc ctttctgtcg cgccccgaag aagtgactct gcacatgcaa gttcacttgc ggaactgcgg tctctttcca gatttgcatt tgatagagag acaagtttca gacagcagct gcttctccag cgaaagagcc ttgtcttcct cgtatagcag atctgcttcc tcatgttaca actcggcccg caaccactct tcgttggctg gcggctcttc tgcagacaac gctttctaca actcttcgtc tttcgaccgc gggagtcacc gaggcgctgc aggctgtttc gtgcctgcgt cttcgcccgc gacttcgcag gcaaccgcga gcgacggagc tgcatccagt cgagggatct cgacgaacag cggcgaacgg tgggaggagc gcgagagaga aaaaacaagc aaatttgctg atgcagacct cgcagcgcag gaaaggtgga atcgcaaaca ctggaaaaat ccattcaaag aagccgacta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a