SSGCID: Target Page

TogoA.18053.a

Oligoendopeptidase F, putative

CENTER ID:	TogoA.18053.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.18053.a.A1.GE33600	full length		1	667

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_226420
RefSeq:	XP_002366295.1
UniProt:	B6KEM2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MISPVASVFS TMPHWKQGAR LHAPGGRFTF AARIYLFLLL SCLGFSCSGE IANGKGTDTT PARLDPRTLP RYNLKRHFPY SSIFDPAIDR DIEALSRRAR EFKERFKGTL ETTLLEALIK HEQLDEAVDF VDYYIALVAA VNTTNEEIRK RVNQLESAIA ADVQPYRLFV NLELGAMPEA ALQKQMAANG NLSFYSGFLG AARRRAPYVL SEDVERALLV RKPMTVQVAT EYYFKQLGDA TFDMNGEKLI LSMILAFIMD NSQEKRWAAQ KAVKQGLERH KITNFAALSL NVVAGSWHME RKERGYERLR SSRNVANNVS DKTIDALIHA GETVAVSLTK RYFKLKKKIL KNRAGVEVFD FADRLAPLPL KSSDRLYDWK ESTRIVRDAY KSFSPTMALM FDKLLEEERI DAPAVPGKRT PACYLGTPTT GPFIILEHVG QPRCVETLAH EAGHAIHSML SYEQGNLQLA PPLTIAEMAS LMGERIVFND LLRRTTDPEE RLSHLLRYLD GWTSTVTRQL MFDDFERRVH DARAQGTIPD GAFTTMWKES LEKYFGAEGE VFDSYANSEA DWARIPHFHN TPFYVYAYAF SDLAVGSLYR VYKAHPEGFE QKYLDILRSG NKKSFEEIMK PFGLNPSAET FWADAVEATG GTVIDEAESL ALKLGFV

NT Sequence

atgatttctc cggttgcgtc ggtgttttca acaatgcccc attggaaaca gggtgccagg ctgcacgcgc cggggggccg gtttactttc gcagcgagga tctatctttt tcttcttctt tcttgtttgg gcttctcgtg ctcaggcgag atcgctaacg gaaaagggac ggatactact ccagcacgcc tagatccccg tactctccct cggtacaacc tgaagcgcca tttcccctat tcatcgatct ttgatcctgc aatcgatagg gacatcgaag cgctcagccg cagggcccga gagttcaagg agagattcaa aggcaccctg gaaaccaccc ttctggaggc gctcatcaag cacgagcagc tagatgaagc tgtggacttt gtcgactact acattgccct tgtcgctgca gtgaacacca caaatgagga gattcgcaag cgagtgaatc aattggagtc ggccatagcc gctgatgtgc agccttatag acttttcgtg aatctagagc ttggggcaat gccggaggca gctctgcaaa agcagatggc agcaaatggc aatctcagct tttattccgg ctttcttggc gcggcacgac gacgcgcgcc ttatgttctg tctgaagacg tcgagcgcgc actccttgtt cggaagccca tgactgtgca agttgccact gagtactact tcaagcaact cggcgacgca accttcgaca tgaacggcga gaagctgatt ttgtcgatga tcctcgcgtt cattatggac aacagccaag agaagcgctg ggccgcgcag aaggctgtga agcaaggtct cgaacggcac aagatcacca actttgcagc gctcagttta aacgttgtgg cggggtcgtg gcacatggag agaaaggaga gaggttacga gaggctgcgg tcgtcccgga acgtcgccaa caatgtgtct gacaaaacaa ttgacgcgct catccacgca ggggagactg tcgcagtgtc gttgacgaag cggtacttca agttgaaaaa gaaaattctc aagaaccggg ctggagtgga ggttttcgac ttcgctgatc ggctagctcc attgcccctc aaatccagcg atcgccttta cgactggaaa gaatcaacga gaatcgtccg agatgcgtac aagtccttct cgccgaccat ggccctgatg ttcgacaaac tgcttgagga agagaggatt gacgcccctg ccgttccagg aaaacgcacg ccagcatgct acttgggcac accgacaaca ggccccttca tcattctcga gcatgttgga caaccccgtt gtgtggaaac tctcgctcac gaagccggcc acgcaatcca ttccatgttg tcctacgagc aaggaaacct gcagctcgca ccgcccctga cgattgcaga aatggcgagc ctcatgggag agagaatagt attcaatgac ttgttgcgcc ggacgacgga tcctgaggag cgcctttctc atctgcttcg ctatctagat ggatggacta gcactgttac tcggcagctt atgttcgatg acttcgagag aagagtgcat gatgcccgcg cacaagggac aataccggat ggcgcattca caacaatgtg gaaggagtca cttgagaagt actttggtgc tgaaggcgag gtgtttgact cctatgcgaa ctcagaggcg gattgggccc gcattccaca ctttcataat acgcccttct atgtctacgc gtacgcgttc tccgaccttg ccgtgggctc tctctaccgc gtgtacaagg cacaccccga aggcttcgaa cagaaatacc ttgacatttt gcggtcagga aataaaaaga gcttcgagga gatcatgaag cctttcggtc tgaacccgtc ggctgagaca ttctgggctg acgctgtgga agctacagga ggaacggtaa tcgacgaggc cgagtccctt gccctcaagc tgggctttgt ctga

Details for TogoA.18053.a.A1.GE33600

HARVESTED ON:	10/31/2011
SEQUENCED ON:	11/4/2011
EXPECTED MW:	77kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	87
PERCENT COVERAGE:	35

Validated AA Sequence

Validated NT Sequence

attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgatt tctccggttg cgtcggtgtt ttcaacaatg ccccattgga aacagggtgc caggctgcac gcgccggggg gccggtttac tttcgcagcg aggatctatc tttttcttct tctttcttgt ttgggcttct cgtgctcagg cgagatcgct aacggaaaag ggacggatac tactccagca cgcctagatc cccgtactct ccctcggtac aacctgaagc gccatttccc ctattcatcg atctttgatc ctgcaatcga tagggacatc gaagcgctca gccgcagggc ccgagagttc aaggagagat tcaaaggcac cctggaaacc acccttctgg aggcgctcat caagcacgag cagctagatg aagctgtgga ctttgtcgac tactacattg cccttgtcgc tgcagtgaac accacaaatg aggagattcg caagcgagtg aatcaattgg agtcggccat agccgctgat gtgcagcctt atagactttt cgtgaatcta gagcttgggg caatgccgga gcagctctgc naaagcagat ggcagcaaat ggcaatctca gcttttattc cggctttctt ggcgcggcac gacgacgcgc gccttatgtt ctgtctgaag acgtcnagcg cgcactcctt gttcggaagc ccatgactgt gcaagttgcc actgantact acttcaagca actcggcgac gcnnncntnc gacatgaang annngctgat tttgtcnatg atcntcnnng ttcattatgg ananagcann anaancnnng gnnnnnncan nnnnngtnan nnnnnnnnnn anngnnannn nnanntnnnn ncnnnncant nnancnnnnn nnnggnngnn nnnannannn annnnnnnan gnnnngnngn nnnnntnncn nnnngnnnnn nnnnnnnnnn nnnncncann nnntnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna nnnnnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMISPVASVF STMPHWKQGA RLHAPGGRFT FAARIYLFLL LSCLGFSCSG EIANGKGTDT TPARLDPRTL PRYNLKRHFP YSSIFDPAID RDIEALSRRA REFKERFKGT LETTLLEALI KHEQLDEAVD FVDYYIALVA AVNTTNEEIR KRVNQLESAI AADVQPYRLF VNLELGAMPE AALQKQMAAN GNLSFYSGFL GAARRRAPYV LSEDVERALL VRKPMTVQVA TEYYFKQLGD ATFDMNGEKL ILSMILAFIM DNSQEKRWAA QKAVKQGLER HKITNFAALS LNVVAGSWHM ERKERGYERL RSSRNVANNV SDKTIDALIH AGETVAVSLT KRYFKLKKKI LKNRAGVEVF DFADRLAPLP LKSSDRLYDW KESTRIVRDA YKSFSPTMAL MFDKLLEEER IDAPAVPGKR TPACYLGTPT TGPFIILEHV GQPRCVETLA HEAGHAIHSM LSYEQGNLQL APPLTIAEMA SLMGERIVFN DLLRRTTDPE ERLSHLLRYL DGWTSTVTRQ LMFDDFERRV HDARAQGTIP DGAFTTMWKE SLEKYFGAEG EVFDSYANSE ADWARIPHFH NTPFYVYAYA FSDLAVGSLY RVYKAHPEGF EQKYLDILRS GNKKSFEEIM KPFGLNPSAE TFWADAVEAT GGTVIDEAES LALKLGFV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatttctccg gttgcgtcgg tgttttcaac aatgccccat tggaaacagg gtgccaggct gcacgcgccg gggggccggt ttactttcgc agcgaggatc tatctttttc ttcttctttc ttgtttgggc ttctcgtgct caggcgagat cgctaacgga aaagggacgg atactactcc agcacgccta gatccccgta ctctccctcg gtacaacctg aagcgccatt tcccctattc atcgatcttt gatcctgcaa tcgataggga catcgaagcg ctcagccgca gggcccgaga gttcaaggag agattcaaag gcaccctgga aaccaccctt ctggaggcgc tcatcaagca cgagcagcta gatgaagctg tggactttgt cgactactac attgcccttg tcgctgcagt gaacaccaca aatgaggaga ttcgcaagcg agtgaatcaa ttggagtcgg ccatagccgc tgatgtgcag ccttatagac ttttcgtgaa tctagagctt ggggcaatgc cggaggcagc tctgcaaaag cagatggcag caaatggcaa tctcagcttt tattccggct ttcttggcgc ggcacgacga cgcgcgcctt atgttctgtc tgaagacgtc gagcgcgcac tccttgttcg gaagcccatg actgtgcaag ttgccactga gtactacttc aagcaactcg gcgacgcaac cttcgacatg aacggcgaga agctgatttt gtcgatgatc ctcgcgttca ttatggacaa cagccaagag aagcgctggg ccgcgcagaa ggctgtgaag caaggtctcg aacggcacaa gatcaccaac tttgcagcgc tcagtttaaa cgttgtggcg gggtcgtggc acatggagag aaaggagaga ggttacgaga ggctgcggtc gtcccggaac gtcgccaaca atgtgtctga caaaacaatt gacgcgctca tccacgcagg ggagactgtc gcagtgtcgt tgacgaagcg gtacttcaag ttgaaaaaga aaattctcaa gaaccgggct ggagtggagg ttttcgactt cgctgatcgg ctagctccat tgcccctcaa atccagcgat cgcctttacg actggaaaga atcaacgaga atcgtccgag atgcgtacaa gtccttctcg ccgaccatgg ccctgatgtt cgacaaactg cttgaggaag agaggattga cgcccctgcc gttccaggaa aacgcacgcc agcatgctac ttgggcacac cgacaacagg ccccttcatc attctcgagc atgttggaca accccgttgt gtggaaactc tcgctcacga agccggccac gcaatccatt ccatgttgtc ctacgagcaa ggaaacctgc agctcgcacc gcccctgacg attgcagaaa tggcgagcct catgggagag agaatagtat tcaatgactt gttgcgccgg acgacggatc ctgaggagcg cctttctcat ctgcttcgct atctagatgg atggactagc actgttactc ggcagcttat gttcgatgac ttcgagagaa gagtgcatga tgcccgcgca caagggacaa taccggatgg cgcattcaca acaatgtgga aggagtcact tgagaagtac tttggtgctg aaggcgaggt gtttgactcc tatgcgaact cagaggcgga ttgggcccgc attccacact ttcataatac gcccttctat gtctacgcgt acgcgttctc cgaccttgcc gtgggctctc tctaccgcgt gtacaaggca caccccgaag gcttcgaaca gaaatacctt gacattttgc ggtcaggaaa taaaaagagc ttcgaggaga tcatgaagcc tttcggtctg aacccgtcgg ctgagacatt ctgggctgac gctgtggaag ctacaggagg aacggtaatc gacgaggccg agtcccttgc cctcaagctg ggctttgtct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa