TogoA.18046.a

Aminopeptidase N, putative (EC 3.4.11.2)

CENTER ID: TogoA.18046.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.18046.a.A2.GE33592 mature protein 28 970
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_224460
RefSeq: XP_002366121.1
UniProt: B6KE48

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVTLSATTMN ATVSPCRISG GVSSRLCGWG VLSFLLASVI TVQVSRAGEE KEEPKAVVRL DYKPPNFYLE DIVLDFNLDD EDTTVEALLT LYRRAGTEPM NLVLDGDESV ILKSISLATT SSPSLRGQGR VTFTELDGQM PTDSQFQFRI VDGNLMVSKF LLPREAEVRF YIRTLVSINP KANLALFGLY KAGDIFVTLN EPSGFRRITY GVDRPDVLAT YTVTVTAPRH LPILLSNGDK VLSGYAGENR HFAKFVDPFP KPSYLFALVA GDFASVSGEF VTMSGRSVTV TIYAQHHQRN QLQWALRSLL RAMRWDEETF GREYQYSEFR VLCVEVFNPG AMENTSLNIF TCSLLLADPK LTTDADHRLI VDVVSHEYFH NWTGNRVTVQ DWFQLTLKEG LTMFRNNSFT EETTSRAMKR IGDVADILSI QFREDSGPFA HPIRPETYKS IENLYTRTVY LKGAEVNRMY RTMLGPKGFR RGMDLYFKRH DGKAVTCDDL RAAMADANDK DLSQFERWYS QAGTPHVTVS SFIYDAAERK MHLTLKQHTP PTPGQDKKLP LQIPVAVGCI GKTSKRDVLS PPTQILELTE EEQAFVLVDV GEDCVLSVLR DFSAPVKLIQ PLQTDEDLAF LVTYDSDNVN RWQAAQTLSR KVLLTRISEY QQRVEEIEEG RVSESDLFSK LPTQYVQTVR DIVIAPESSM GKDIKSLLLS LPTKAQLELA VDSIDPDAIN AALASVRRDI VDALGEEMLQ LYTELTLPAG TEESGADIEH WGRRALRNEL LRFLTASFDQ KSAKLASAHF DRAMVMSDKV AALTVLTEIP GQERDEAFER FRQEVAGNAV LMEKWMSLQA MSAAADTLER VAQLRQHSAF HPFIPGSAYS LFGTFSSSSP QFHSKTGAGY ALVLDFLTMI DEHNSLVSSR IAGVAFANWK RFTSPRREML KEVLKKLLKK EKLSVGLKEV LEKALAGEDD
NT Sequence
atggtgaccc tgtcggctac gaccatgaat gcgacggtgt caccatgtcg gatctccgga ggtgtttcct cgagactatg cggttggggt gttctcagct ttctgcttgc atccgttatt acggtgcagg tttctagagc aggcgaggaa aaggaagaac ccaaggccgt cgtgagattg gactacaagc ctccgaattt ctaccttgaa gacatcgttt tggacttcaa cttggacgac gaagacacaa ctgtcgaagc tctcctgact ctttaccgtc gcgccggcac cgaaccaatg aaccttgtcc tcgatggaga tgagagtgtg atcttgaaga gcatctcttt ggcgactacc tcgtcaccct ctctgcgtgg tcaaggtcgt gtcaccttca ccgaactgga cgggcaaatg cccacggaca gccagtttca gtttcgcatt gttgatggta acctgatggt gtcgaaattc cttctgccca gggaagcaga agtacgcttc tacatcagaa ctctggtgtc catcaacccc aaggcgaacc tggcactctt cggtctctac aaggcgggtg atattttcgt cactctcaac gaaccctcgg gattccgcag aatcacctac ggcgtcgatc gacccgacgt cctggctacc tacacggtta ccgtgactgc ccctcgtcac ctcccgattc tcctgagcaa tggagacaaa gtgctttctg gatatgccgg agaaaatcgc cactttgcga agttcgtgga cccattcccg aagccctctt acctttttgc gttggttgct ggtgacttcg cgtcagtcag tggcgaattt gtcacgatgt ctggacgcag cgtgaccgtg acgatatacg cgcagcatca ccagaggaat caactccaat gggctcttcg ttccctgttg cgggcgatgc gctgggacga agagacattc ggcagagagt accagtattc ggagttccgt gtgttgtgcg tcgaagtctt caaccctggc gcgatggaaa acacgtcact caacatcttc acgtgcagtc ttttgcttgc cgatcccaag ctcaccacgg atgctgacca ccggctgatt gtcgatgtcg tcagtcacga gtactttcac aactggactg gaaaccgcgt tactgtccag gattggttcc agctgactct gaaggagggt ttgacgatgt tccgcaacaa cagcttcact gaggaaacga cgtcgcgagc gatgaagaga attggagatg tcgccgatat cctcagcatc cagttccgcg aagatagtgg tcccttcgcg catccgattc gtcccgaaac gtacaagtcg attgagaatc tctacacccg aacagtctac ctgaagggtg ccgaggttaa tcgcatgtac cgaacaatgt tggggccgaa gggctttcgc agagggatgg atttgtactt caaacgtcac gacggaaagg cggtgacttg cgacgacttg cgcgccgcga tggcagacgc gaacgacaaa gatttgagcc agtttgaaag gtggtactct caggccggga ctccacatgt caccgtcagc agttttatct acgacgcagc ggagcgaaag atgcatttga cgctcaagca acacaccccg ccgacgccgg gacaggacaa gaaactccct ctccagattc ccgtcgctgt tggatgtatt ggcaaaacga gcaaacgaga tgtgctttcc ccaccgactc aaattctcga actaacggaa gaggagcaag cgttcgtgtt ggtcgacgtc ggagaagact gcgtgctctc tgttctgcgc gacttttcgg cgcccgtcaa gctcatccag cccctccaga cagacgagga cttggcattc ctggtgacgt acgattcaga caatgtcaac aggtggcaag cggcacaaac gctctcgcgc aaagtgttgc tcacgcgcat ttccgagtac caacagagag tggaagaaat cgaggaggga cgtgtgagtg agagcgactt gttttccaag cttcccaccc agtacgtgca gaccgttcgc gatattgtga tcgcccctga atcctccatg ggcaaagaca tcaagtctct gctgctgagt ctgccgacaa aagcgcagct ggaactggca gtcgacagca tcgatcccga cgccatcaat gcagccttgg catccgtcag gagagacatc gtcgatgcct tgggggaaga gatgctgcag ctgtacacgg agctcacgct accggcaggc accgaggagt ctggagctga catcgagcac tggggcagac gcgctctccg gaacgagctc ttgcggttcc tgactgcgtc atttgaccag aagtcggcga aactcgcttc tgcccacttc gaccgtgcca tggtcatgtc tgacaaagtt gcggcgttga ctgtgctcac ggaaattcca ggacaagaaa gggatgaagc tttcgagcgt tttcgccagg aagtcgccgg caacgcggtg ctgatggaga agtggatgag cctgcaagcg atgtcggctg cggcagacac gctggagcgg gtcgcgcaac tgcgccagca cagcgcgttt cacccgttta tccccggttc cgcgtactct ctcttcggca ctttcagctc gtccagtccg cagttccaca gcaagactgg cgcaggctat gccttggttc ttgattttct gacgatgatc gacgagcaca actcgctggt gtcttcccgg atcgctggcg tggcgttcgc gaactggaag cgattcactt ctccacgaag ggagatgctg aaggaagtgc tgaagaagct gctcaagaag gagaagctct ctgtcggtct gaaagaagtc ctggagaagg cccttgccgg ggaagacgac tga
Details for TogoA.18046.a.A2.GE33592
HARVESTED ON: 10/31/2011
SEQUENCED ON: 11/4/2011
EXPECTED MW: 108kDa
OBSERVED MW: 108kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 65
PERCENT COVERAGE: 33
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGWGVLSFL LASVITVQVS RAGEEKEEPK AVVRLDYKPP NFYLEDIVLD FNLDDEDTTV EALLTLYRRA GTEPMNLVLD GDESVILKSI SLATTSSPSL RGQGRVTFTE LDGQMPTDSQ FQFRIVDGNL MVSKFLLPRE AEVRFYIRTL VSINPKANLA LFGLYKAGDI FVTLNEXSGF RRXTXXVDRP XVXXPTRXPX LXXVXXXXXX XXXXXFXXXX XKXXXXXXXX XXPXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX GXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggtt ggggtgttct cagctttctg cttgcatccg ttattacggt gcaggtttct agagcaggcg aggaaaagga agaacccaag gccgtcgtga gattggacta caagcctccg aatttctacc ttgaagacat cgttttggac ttcaacttgg acgacgaaga cacaactgtc gaagctctcc tgactcttta ccgtcgcgcc ggcaccgaac caatgaacct tgtcctcgat ggagatgaga gtgtgatctt gaagagcatc tctttggcga ctacctcgtc accctctctg cgtggtcaag gtcgtgtcac cttcaccgaa ctggacgggc aaatgcccac ggacagccag tttcagtttc gcattgttga tggtaacctg atggtgtcga aattccttct gcccagggaa gcagaagtac gcttctacat cagaactctg gtgtccatca accccaaggc gaacctggca ctcttcggtc tctacaaggc gggtgatatt ttcgtcactc tcaacgaanc ctcnggattc cgcagaanca cctannnngt cgatcgaccc nacgtcnggn tacctacacg nntaccgnga ctgncccncg tcancnnccn atnnnncnna ncanggnnna nngntttctg nnnnngnnnn anaaaannnn cnnnntngcn annncnnnnn cnnaanccnn nntnnntttt nnnnnnnnng nnggnnnnnn nnnnnnnnnn ngnnnaantt nnnnnnnnnn nnnnnngnnc ncnnnnnnnn ngnnnnnnnn tnncnnnnnn ncnnnnncna ggnnnnnnnn nncnnnnnnn nnnnncnnnn cannnnnntn nnnnnnnnnc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngngna annnnnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGWGVLSFL LASVITVQVS RAGEEKEEPK AVVRLDYKPP NFYLEDIVLD FNLDDEDTTV EALLTLYRRA GTEPMNLVLD GDESVILKSI SLATTSSPSL RGQGRVTFTE LDGQMPTDSQ FQFRIVDGNL MVSKFLLPRE AEVRFYIRTL VSINPKANLA LFGLYKAGDI FVTLNEPSGF RRITYGVDRP DVLATYTVTV TAPRHLPILL SNGDKVLSGY AGENRHFAKF VDPFPKPSYL FALVAGDFAS VSGEFVTMSG RSVTVTIYAQ HHQRNQLQWA LRSLLRAMRW DEETFGREYQ YSEFRVLCVE VFNPGAMENT SLNIFTCSLL LADPKLTTDA DHRLIVDVVS HEYFHNWTGN RVTVQDWFQL TLKEGLTMFR NNSFTEETTS RAMKRIGDVA DILSIQFRED SGPFAHPIRP ETYKSIENLY TRTVYLKGAE VNRMYRTMLG PKGFRRGMDL YFKRHDGKAV TCDDLRAAMA DANDKDLSQF ERWYSQAGTP HVTVSSFIYD AAERKMHLTL KQHTPPTPGQ DKKLPLQIPV AVGCIGKTSK RDVLSPPTQI LELTEEEQAF VLVDVGEDCV LSVLRDFSAP VKLIQPLQTD EDLAFLVTYD SDNVNRWQAA QTLSRKVLLT RISEYQQRVE EIEEGRVSES DLFSKLPTQY VQTVRDIVIA PESSMGKDIK SLLLSLPTKA QLELAVDSID PDAINAALAS VRRDIVDALG EEMLQLYTEL TLPAGTEESG ADIEHWGRRA LRNELLRFLT ASFDQKSAKL ASAHFDRAMV MSDKVAALTV LTEIPGQERD EAFERFRQEV AGNAVLMEKW MSLQAMSAAA DTLERVAQLR QHSAFHPFIP GSAYSLFGTF SSSSPQFHSK TGAGYALVLD FLTMIDEHNS LVSSRIAGVA FANWKRFTSP RREMLKEVLK KLLKKEKLSV GLKEVLEKAL AGEDD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggttggggt gttctcagct ttctgcttgc atccgttatt acggtgcagg tttctagagc aggcgaggaa aaggaagaac ccaaggccgt cgtgagattg gactacaagc ctccgaattt ctaccttgaa gacatcgttt tggacttcaa cttggacgac gaagacacaa ctgtcgaagc tctcctgact ctttaccgtc gcgccggcac cgaaccaatg aaccttgtcc tcgatggaga tgagagtgtg atcttgaaga gcatctcttt ggcgactacc tcgtcaccct ctctgcgtgg tcaaggtcgt gtcaccttca ccgaactgga cgggcaaatg cccacggaca gccagtttca gtttcgcatt gttgatggta acctgatggt gtcgaaattc cttctgccca gggaagcaga agtacgcttc tacatcagaa ctctggtgtc catcaacccc aaggcgaacc tggcactctt cggtctctac aaggcgggtg atattttcgt cactctcaac gaaccctcgg gattccgcag aatcacctac ggcgtcgatc gacccgacgt cctggctacc tacacggtta ccgtgactgc ccctcgtcac ctcccgattc tcctgagcaa tggagacaaa gtgctttctg gatatgccgg agaaaatcgc cactttgcga agttcgtgga cccattcccg aagccctctt acctttttgc gttggttgct ggtgacttcg cgtcagtcag tggcgaattt gtcacgatgt ctggacgcag cgtgaccgtg acgatatacg cgcagcatca ccagaggaat caactccaat gggctcttcg ttccctgttg cgggcgatgc gctgggacga agagacattc ggcagagagt accagtattc ggagttccgt gtgttgtgcg tcgaagtctt caaccctggc gcgatggaaa acacgtcact caacatcttc acgtgcagtc ttttgcttgc cgatcccaag ctcaccacgg atgctgacca ccggctgatt gtcgatgtcg tcagtcacga gtactttcac aactggactg gaaaccgcgt tactgtccag gattggttcc agctgactct gaaggagggt ttgacgatgt tccgcaacaa cagcttcact gaggaaacga cgtcgcgagc gatgaagaga attggagatg tcgccgatat cctcagcatc cagttccgcg aagatagtgg tcccttcgcg catccgattc gtcccgaaac gtacaagtcg attgagaatc tctacacccg aacagtctac ctgaagggtg ccgaggttaa tcgcatgtac cgaacaatgt tggggccgaa gggctttcgc agagggatgg atttgtactt caaacgtcac gacggaaagg cggtgacttg cgacgacttg cgcgccgcga tggcagacgc gaacgacaaa gatttgagcc agtttgaaag gtggtactct caggccggga ctccacatgt caccgtcagc agttttatct acgacgcagc ggagcgaaag atgcatttga cgctcaagca acacaccccg ccgacgccgg gacaggacaa gaaactccct ctccagattc ccgtcgctgt tggatgtatt ggcaaaacga gcaaacgaga tgtgctttcc ccaccgactc aaattctcga actaacggaa gaggagcaag cgttcgtgtt ggtcgacgtc ggagaagact gcgtgctctc tgttctgcgc gacttttcgg cgcccgtcaa gctcatccag cccctccaga cagacgagga cttggcattc ctggtgacgt acgattcaga caatgtcaac aggtggcaag cggcacaaac gctctcgcgc aaagtgttgc tcacgcgcat ttccgagtac caacagagag tggaagaaat cgaggaggga cgtgtgagtg agagcgactt gttttccaag cttcccaccc agtacgtgca gaccgttcgc gatattgtga tcgcccctga atcctccatg ggcaaagaca tcaagtctct gctgctgagt ctgccgacaa aagcgcagct ggaactggca gtcgacagca tcgatcccga cgccatcaat gcagccttgg catccgtcag gagagacatc gtcgatgcct tgggggaaga gatgctgcag ctgtacacgg agctcacgct accggcaggc accgaggagt ctggagctga catcgagcac tggggcagac gcgctctccg gaacgagctc ttgcggttcc tgactgcgtc atttgaccag aagtcggcga aactcgcttc tgcccacttc gaccgtgcca tggtcatgtc tgacaaagtt gcggcgttga ctgtgctcac ggaaattcca ggacaagaaa gggatgaagc tttcgagcgt tttcgccagg aagtcgccgg caacgcggtg ctgatggaga agtggatgag cctgcaagcg atgtcggctg cggcagacac gctggagcgg gtcgcgcaac tgcgccagca cagcgcgttt cacccgttta tccccggttc cgcgtactct ctcttcggca ctttcagctc gtccagtccg cagttccaca gcaagactgg cgcaggctat gccttggttc ttgattttct gacgatgatc gacgagcaca actcgctggt gtcttcccgg atcgctggcg tggcgttcgc gaactggaag cgattcactt ctccacgaag ggagatgctg aaggaagtgc tgaagaagct gctcaagaag gagaagctct ctgtcggtct gaaagaagtc ctggagaagg cccttgccgg ggaagacgac taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa