TogoA.18030.a

Cullin 3, putative

CENTER ID: TogoA.18030.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.18030.a.A2.GE33597 mature protein 24 929
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_201770
RefSeq: XP_002367473.1
UniProt: B6KIP5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRSLASAGNA GRPPGGPAAS LLRPSSSSSS SSSSSSSSSF SSSSSSFSFS SSSDFGLFGH KAMTGDEAAR RWEFLRAAFQ HIFAKKAGAL SYEEVYRYAY LLVINKRGRL LYDGARQCVE GHLQAVASEI AEKDDDDLFL AALLEKWRDH RVNMQMIKDV LLYLDKHYVE MHHLTPSFSM GMSLFCSTVL QHPNIQLRFR HLLLDRIRKE REGHQIDRMV MREATAMLSQ LRLHAHRPVY TEEFEAPFLA ATREFYTREA ADFIAYNSSP EFLQKAESRI REEARRVEEY LDLETTAPLR ALMEDVWLGQ HFKTLVYNPN SGCTHLFQAD KIADLARMHR LFSSVPGALE EVRQVMKESI TKYGEAIVRD PEKTRDPVTF VQNFLALKAK FDQIVVRAFA ESKEAFGALV AAFENVLNKD TRAARFLSLY LDDLFRKSMR GLSEAEAEQK LDEVLVLFRY LRDKDIFEAL YKQHLARRLL GGRSASEEEE KKMIVKLKAE CGQQYTSKLE VMYRDMQASD DIMRQYHARE DLQRDTACPV SLLDARLLQL GRAIARRAGT FASASGSFSA SALHQPVDDT VRSPNDPNAS DSPESPAFTT PLPSSSSASS SSSASSSSSA SSSSSSSSSS SASSFAPCPP SSSVWGCEVP QGRGEFEFSV RVITQGTWPV DAQTLISETQ LLPSLLAEEA TRFEAFYLGR HNGRVLRWNL AQGKASVRGQ LLHSRHEFEC TTLQMIFLLA FNFFPSTPVS VSDLLQPFFA VSSSLSRAEL KRHLISLTTP RCKILLRASS GAGAANAAEL KDSDTLTVNL SYTNKLRRVR VPLVAVPAAA GSGEAADAWA TPHLEAQAGA DVPSSVEQDR NYLVEAAIVR VMKTRRRMIH NDLLVEVTRH LAQRFRPSPA LIKQRVEKLI EREFLERDAA DRRMYNYLA
NT Sequence
atgaggtccc tcgcctctgc cggcaatgca ggccgccccc cgggtggtcc tgccgcctcg cttctccgcc cttcctcttc ctcttcctct tcttcttctt cctcttcttc ttcctccttt tcttcctctt cttcctcttt ttctttttcc tccagctcgg atttcggact ttttggccac aaggcgatga ccggcgacga agccgctcga cgttgggagt ttctgcgcgc agcgtttcag cacattttcg ctaaaaaagc tggggccctc agctacgagg aagtctaccg atacgcctac ttgttggtca tcaacaagag agggcgactc ctgtacgatg gagctcgaca gtgcgtggaa gggcatctgc aagcggtggc aagtgaaatt gcggagaagg atgacgacga cctctttctc gcggctctgc tggagaagtg gagagatcac cgcgtaaaca tgcagatgat caaagacgtt ctgctttact tagacaaaca ctacgtcgag atgcatcact tgacgccttc gttttcgatg gggatgagtc tcttctgctc cacggttctg cagcacccga acattcagct cagatttcgg caccttcttc tcgacagaat tcgcaaagag agagagggcc atcaaattga ccgaatggtg atgcgggagg cgactgcgat gctgtcccag ttgcgtctgc atgctcatcg gccggtttac acggaggagt ttgaggcacc tttcctggct gcgacgcgcg aattttacac tcgcgaagca gcggacttca tcgcgtacaa ttcctctcca gagttccttc agaaggcaga aagccgcatt cgcgaggaag cgagacgcgt ggaagagtat cttgacctgg agacgactgc gcctttacga gcgctcatgg aggacgtttg gctgggccaa cacttcaaga ctctcgtcta taatccgaac tcaggctgca cgcatttatt tcaggcagat aaaattgcag acctcgcgcg catgcaccgt ctcttctcct cggtccctgg cgccctcgag gaagttagac aagtgatgaa agagagcatc acgaaatacg gggaggccat cgtccgcgac ccggagaaga ctcgcgatcc cgtgacgttt gtgcagaatt tcctcgcgct gaaggccaag ttcgaccaga tcgtcgttcg cgcctttgca gaaagcaaag aggccttcgg cgcgctcgtg gcggcatttg aaaacgttct gaacaaagac acgcgagcgg cgcgcttcct ctcgctttac ctcgacgact tgtttcgaaa aagcatgcgc ggactgtccg aagcggaggc ggagcagaaa ctagacgaag tgctcgtgct cttccggtac ctgcgagaca aagacatctt cgaggccctg tacaagcaac acctcgcccg tcgtctcctg ggaggccgct ccgcgtctga agaagaggag aagaagatga ttgtcaaact gaaggctgaa tgcggacaac agtacacgtc gaagctcgag gtcatgtacc gagacatgca ggcatccgat gacattatgc ggcaatacca cgctagagaa gatctccaga gagacactgc ctgtcctgtc tcgctgttgg acgcgcgcct cctgcaactc ggtcgcgcga ttgcgcgtcg cgcagggact ttcgcctctg cttctggcag tttttctgcc tccgctctcc accaacctgt agacgacaca gtccggtcgc cgaacgaccc caacgcatcc gacagtcccg agtcgccggc cttcaccacg cctctgcctt cttcctcttc tgcttcttct tcctcttccg cttcttcttc ctcttctgct tcttcttcct cttcttcttc ttcctcttct tctgcttcct ccttcgcgcc ttgtcctccc tcttcttctg tgtggggatg cgaggttccg caaggtcgtg gcgagttcga gttttcggtg cgcgtgatca cacaggggac atggccagtg gatgcgcaga cgctgatttc cgaaactcaa cttttgccga gtctcctggc cgaagaagcg acgcgcttcg aggccttcta cctggggcgt cacaacggcc gggtgctcag gtggaacctc gcgcaaggaa aggcgagtgt tcgcggccag ttgctccaca gtcgccacga gttcgagtgc acgacgttgc agatgatttt tcttctcgca ttcaactttt tcccttccac gccggtctcc gtcagtgacc ttctccagcc tttcttcgct gtctcttcct ccctctctcg cgcggagtta aagagacatc tcatcagcct caccacgcct cgatgcaaaa tcttgctgcg agcctcatct ggtgcaggag cggcgaatgc ggccgagctg aaagactcgg acactctcac ggtcaatcta agctacacga acaagctcag gcgagttcga gttcccctcg tcgcggtgcc cgcggccgct ggaagtggag aggccgcaga cgcgtgggcg actccgcatt tggaggcgca agctggcgcc gatgtcccgt cgagtgtcga gcaggaccga aactacctgg tcgaagctgc gatcgtgagg gtgatgaaga ctcgcagacg gatgattcac aatgacctgc tcgtggaagt cactcgacac ctggcgcagc gatttcgccc ctcgcctgcc ctcatcaaac agcgagtaga aaaactcatc gagagagaat ttctcgaaag agatgccgcc gacaggcgaa tgtacaacta cctggcgtag
Details for TogoA.18030.a.A2.GE33597
HARVESTED ON: 10/31/2011
SEQUENCED ON: 11/4/2011
EXPECTED MW: 103kDa
OBSERVED MW: 103kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 26
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPSSSSSSS SSSSSSSSFS SSSSSFSFSS SSDFGLFGHK AMTGDEAARR WEFLRAAFQH IFAKKAGALS YEEVYRYAYL LVINKRGRLL YDGARQCVEG HLQAVASEIA EKDDDDLFLA ALLEKWRDHR VNMQMIKDVL LYLDKHYVEM HHLTPSFSMG MSLFCSTVLQ HPNIQLRFRH LLLDRIRKER EGHQIDRMVM REXTAMLSQL RLHAHRPGLH GGV
Validated NT Sequence
ttttgtnnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgccttc ctcttcctct tcctcttctt cttcttcctc ttcttcttcc tccttttctt cctcttcttc ctctttttct ttttcctcca gctcggattt cggacttttt ggccacaagg cgatgaccgg cgacgaagcc gctcgacgtt gggagtttct gcgcgcagcg tttcagcaca ttttcgctaa aaaagctggg gccctcagct acgaggaagt ctaccgatac gcctacttgt tggtcatcaa caagagaggg cgactcctgt acgatggagc tcgacagtgc gtggaagggc atctgcaagc ggtggcaagt gaaattgcgg agaaggatga cgacgacctc tttctcgcgg ctctgctgga gaagtggaga gatcaccgcg taaacatgca gatgatcaaa gacgttctgc tttacttaga caaacactac gtcgagatgc atcacttgac gccttcgttt tcgatgggga tgagtctctt ctgctccacg gttctgcagc acccgaacat tcagctcaga tttcggcacc ttcttctcga cagaattcgc aaagagagag agggncatca aattgaccga atggtgatgc gggaggngac tgcgatgctg tcccagttgc gtctgcatgc tcatcggccn ggtttacacg gaggagtttg angcannttt nctggctgcg acncncgant ttacactcnc gaagcancng anttcatcgc gnacnannnn tcnncnnant nnnnnnnnnn nnaannncnn nnnnngnanc nannnnnnnn gnnannannt gncnnnnacn acngnnnntn annnnngnnc angnangnng tnnnngnnnn nnnnnngann nnnnnnnnnn nnnannnnng ntgnnnngnn nnnannnnnn nnngnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncnn nnnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPSSSSSSS SSSSSSSSFS SSSSSFSFSS SSDFGLFGHK AMTGDEAARR WEFLRAAFQH IFAKKAGALS YEEVYRYAYL LVINKRGRLL YDGARQCVEG HLQAVASEIA EKDDDDLFLA ALLEKWRDHR VNMQMIKDVL LYLDKHYVEM HHLTPSFSMG MSLFCSTVLQ HPNIQLRFRH LLLDRIRKER EGHQIDRMVM REATAMLSQL RLHAHRPVYT EEFEAPFLAA TREFYTREAA DFIAYNSSPE FLQKAESRIR EEARRVEEYL DLETTAPLRA LMEDVWLGQH FKTLVYNPNS GCTHLFQADK IADLARMHRL FSSVPGALEE VRQVMKESIT KYGEAIVRDP EKTRDPVTFV QNFLALKAKF DQIVVRAFAE SKEAFGALVA AFENVLNKDT RAARFLSLYL DDLFRKSMRG LSEAEAEQKL DEVLVLFRYL RDKDIFEALY KQHLARRLLG GRSASEEEEK KMIVKLKAEC GQQYTSKLEV MYRDMQASDD IMRQYHARED LQRDTACPVS LLDARLLQLG RAIARRAGTF ASASGSFSAS ALHQPVDDTV RSPNDPNASD SPESPAFTTP LPSSSSASSS SSASSSSSAS SSSSSSSSSS ASSFAPCPPS SSVWGCEVPQ GRGEFEFSVR VITQGTWPVD AQTLISETQL LPSLLAEEAT RFEAFYLGRH NGRVLRWNLA QGKASVRGQL LHSRHEFECT TLQMIFLLAF NFFPSTPVSV SDLLQPFFAV SSSLSRAELK RHLISLTTPR CKILLRASSG AGAANAAELK DSDTLTVNLS YTNKLRRVRV PLVAVPAAAG SGEAADAWAT PHLEAQAGAD VPSSVEQDRN YLVEAAIVRV MKTRRRMIHN DLLVEVTRHL AQRFRPSPAL IKQRVEKLIE REFLERDAAD RRMYNYLA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccttcctct tcctcttcct cttcttcttc ttcctcttct tcttcctcct tttcttcctc ttcttcctct ttttcttttt cctccagctc ggatttcgga ctttttggcc acaaggcgat gaccggcgac gaagccgctc gacgttggga gtttctgcgc gcagcgtttc agcacatttt cgctaaaaaa gctggggccc tcagctacga ggaagtctac cgatacgcct acttgttggt catcaacaag agagggcgac tcctgtacga tggagctcga cagtgcgtgg aagggcatct gcaagcggtg gcaagtgaaa ttgcggagaa ggatgacgac gacctctttc tcgcggctct gctggagaag tggagagatc accgcgtaaa catgcagatg atcaaagacg ttctgcttta cttagacaaa cactacgtcg agatgcatca cttgacgcct tcgttttcga tggggatgag tctcttctgc tccacggttc tgcagcaccc gaacattcag ctcagatttc ggcaccttct tctcgacaga attcgcaaag agagagaggg ccatcaaatt gaccgaatgg tgatgcggga ggcgactgcg atgctgtccc agttgcgtct gcatgctcat cggccggttt acacggagga gtttgaggca cctttcctgg ctgcgacgcg cgaattttac actcgcgaag cagcggactt catcgcgtac aattcctctc cagagttcct tcagaaggca gaaagccgca ttcgcgagga agcgagacgc gtggaagagt atcttgacct ggagacgact gcgcctttac gagcgctcat ggaggacgtt tggctgggcc aacacttcaa gactctcgtc tataatccga actcaggctg cacgcattta tttcaggcag ataaaattgc agacctcgcg cgcatgcacc gtctcttctc ctcggtccct ggcgccctcg aggaagttag acaagtgatg aaagagagca tcacgaaata cggggaggcc atcgtccgcg acccggagaa gactcgcgat cccgtgacgt ttgtgcagaa tttcctcgcg ctgaaggcca agttcgacca gatcgtcgtt cgcgcctttg cagaaagcaa agaggccttc ggcgcgctcg tggcggcatt tgaaaacgtt ctgaacaaag acacgcgagc ggcgcgcttc ctctcgcttt acctcgacga cttgtttcga aaaagcatgc gcggactgtc cgaagcggag gcggagcaga aactagacga agtgctcgtg ctcttccggt acctgcgaga caaagacatc ttcgaggccc tgtacaagca acacctcgcc cgtcgtctcc tgggaggccg ctccgcgtct gaagaagagg agaagaagat gattgtcaaa ctgaaggctg aatgcggaca acagtacacg tcgaagctcg aggtcatgta ccgagacatg caggcatccg atgacattat gcggcaatac cacgctagag aagatctcca gagagacact gcctgtcctg tctcgctgtt ggacgcgcgc ctcctgcaac tcggtcgcgc gattgcgcgt cgcgcaggga ctttcgcctc tgcttctggc agtttttctg cctccgctct ccaccaacct gtagacgaca cagtccggtc gccgaacgac cccaacgcat ccgacagtcc cgagtcgccg gccttcacca cgcctctgcc ttcttcctct tctgcttctt cttcctcttc cgcttcttct tcctcttctg cttcttcttc ctcttcttct tcttcctctt cttctgcttc ctccttcgcg ccttgtcctc cctcttcttc tgtgtgggga tgcgaggttc cgcaaggtcg tggcgagttc gagttttcgg tgcgcgtgat cacacagggg acatggccag tggatgcgca gacgctgatt tccgaaactc aacttttgcc gagtctcctg gccgaagaag cgacgcgctt cgaggccttc tacctggggc gtcacaacgg ccgggtgctc aggtggaacc tcgcgcaagg aaaggcgagt gttcgcggcc agttgctcca cagtcgccac gagttcgagt gcacgacgtt gcagatgatt tttcttctcg cattcaactt tttcccttcc acgccggtct ccgtcagtga ccttctccag cctttcttcg ctgtctcttc ctccctctct cgcgcggagt taaagagaca tctcatcagc ctcaccacgc ctcgatgcaa aatcttgctg cgagcctcat ctggtgcagg agcggcgaat gcggccgagc tgaaagactc ggacactctc acggtcaatc taagctacac gaacaagctc aggcgagttc gagttcccct cgtcgcggtg cccgcggccg ctggaagtgg agaggccgca gacgcgtggg cgactccgca tttggaggcg caagctggcg ccgatgtccc gtcgagtgtc gagcaggacc gaaactacct ggtcgaagct gcgatcgtga gggtgatgaa gactcgcaga cggatgattc acaatgacct gctcgtggaa gtcactcgac acctggcgca gcgatttcgc ccctcgcctg ccctcatcaa acagcgagta gaaaaactca tcgagagaga atttctcgaa agagatgccg ccgacaggcg aatgtacaac tacctggcgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa