TogoA.18023.a

Amidohydrolase domain-containing protein (EC 3.5.1.32)

CENTER ID:	TogoA.18023.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.18023.a.A1.GE33596	full length		1	514

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_213520
OrthoMCL:	OG5_127543
RefSeq:	XP_002371089.1
UniProt:	B6KTB6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPRGGHKKMA SKTFWNRGII FLRSYSFFFL PLLAAAASSP ASDDKYIIPD GELQNLLAVP TNLSSLSNSG SLVDFSTLFE AASETSFNAW IVAVRRALHQ WPETAYNEYR TSALIHKLLK AMNVRVTTGW GTNTIGMSEE EAKIARARRE GTGLVAEIGT GKEPCVALRA DIDALPIFER TNVPFRSKVD GQMHACGHDV HTTMLLGAAA LLKQLEPHME GTIRLIFQPA EEGGGGALMM REEGVLTMAP PVEFIFGMHV APALPTGELA TRKGAMMAAA TQFSINVKGR GGHGAVPHET IDPSPGVAAI VQGLYAIVAR ETSFTENTTG LISVTRIQGG TAFNVIPSEY FIGGTIRALD MAMMRNLQAR VVELVENLAQ AFRCQADVKY GSVSYVPLVN DPDATEFFIQ TAAPASRSGR VGIADPTLGG EDFAFFLEDV PGTFAVIGIG SGAEHQLGHV PTNIPLHNPN FAVDERVLNR GAAVHAFTAL RAFSFLASKK RNAGAAETRT TCEA

NT Sequence

atgccccgcg gcggccacaa gaaaatggct tcgaaaacct tttggaatcg aggaatcatc tttttacgtt catattcttt ctttttcctt cctctgctgg cggcagccgc tagttcacct gcaagtgacg acaaatatat catcccggac ggcgagctcc agaacctcct tgcggtgcca acgaatctga gctctctgtc gaactctgga tctcttgtcg acttctccac gctctttgaa gcagccagtg aaacttcttt caatgcatgg atcgtggctg tccgtcgagc tcttcaccag tggccagaaa cagcgtacaa tgagtacagg acctctgcac tgattcacaa gcttcttaag gcgatgaacg ttcgggtcac aacaggatgg ggcacgaata caattggcat gagcgaagaa gaagcgaaaa ttgcacgggc gcgacgggaa ggaacgggac tggttgcgga aatcggaaca gggaaggaac cttgtgttgc gctgcgagct gatatcgacg ctcttcctat cttcgaacgg actaacgttc ctttccggtc caaagttgac gggcaaatgc atgcatgtgg ccacgacgtt cataccacga tgcttctggg agcagcggcg ctgcttaagc agctggagcc ccacatggag ggaacgattc gacttatctt ccaacctgcc gaggaaggcg gaggcggggc gctcatgatg agagaagagg gtgttctgac tatggcacca cccgtagaat tcattttcgg gatgcatgtc gccccggcat tacccacagg ggaattggcg acaagaaaag gtgccatgat ggccgccgca acacaattct caatcaatgt gaagggacga ggtggccatg gcgccgtgcc gcatgaaacc atcgacccga gtccaggcgt agcagcgatc gttcagggat tatacgcaat cgtggcgcgg gaaacgtcgt tcacggaaaa cactaccggt cttatctctg tcacccggat ccagggagga acagcattca acgtgatacc atctgaatac ttcattggag gaacgatccg ggccttggac atggcgatga tgcggaacct acaggctaga gttgtagaac tcgtggagaa tttggcccag gcgttcagat gtcaagctga cgtcaaatat gggtccgtca gctacgtacc tcttgtcaat gacccagacg cgacagagtt cttcatccag accgcggcac cggcgtcgcg gagcggaagg gtggggatag cggacccgac gctgggagga gaagatttcg catttttcct cgaagacgtt cccggtacat ttgcagtgat tggcatcggc agtggagctg aacaccagct aggccatgtc cctacaaaca ttcccctcca caaccccaac ttcgctgtcg atgaacgagt gttgaaccgc ggagctgctg tgcacgcgtt cactgcactg agggcctttt cgttcttggc tagcaagaaa cgtaatgctg gggccgcgga aacacgtacg acctgtgaag cctag

Details for TogoA.18023.a.A1.GE33596

HARVESTED ON:	10/31/2011
SEQUENCED ON:	11/7/2011
EXPECTED MW:	57kDa
OBSERVED MW:	57kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	91
PERCENT COVERAGE:	54

Validated AA Sequence

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcccc gcggcggcca caagaaaatg gcttcgaaaa ccttttggaa tcgaggaatc atctttttac gttcatattc tttctttttc cttcctctgc tggcggcagc cgctagttca cctgcaagtg acgacaaata tatcatcccg gacggcgagc tccagaacct ccttgcggtg ccaacgaatc tgagctctct gtcgaactct ggatctcttg tcgacttctc cacgctcttt gaagcagcca gtgaaacttc tttcaatgca tggatcgtgg ctgtccgtcg agctcttcac cagtggccag aaacagcgta caatgagtac aggacctctg cactgattca caagcttctt aaggcgatga acgttcgggt cacaacagga tggggcacga atacaattgg catgagcgaa gaagaagcga aaattgcacg ggcgcgacgg gaaggaacgg gactggttgc ggaaatcgga acagggaagg aaccttgtgt tgcgctgcga gctgatatcg acgctcttcc tatcttcgaa cggactaacg ttcctttccg gtccaaagtt gacgggcaaa tgcatgcatg tggccacgac gttcatacca cgatgcttct gggagcagcg gcgctgctta agcagctgga gccccacatg gagggaacga ntcgacttat cttccnacct gccgaggaan ggcggangcg gggcgctcat gatgagagaa gagggtgttc tgactatggc accacccgta gaattcattt tcnggangca tgtcgccccn gcattaccca caggggaant ggcga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPRGGHKKM ASKTFWNRGI IFLRSYSFFF LPLLAAAASS PASDDKYIIP DGELQNLLAV PTNLSSLSNS GSLVDFSTLF EAASETSFNA WIVAVRRALH QWPETAYNEY RTSALIHKLL KAMNVRVTTG WGTNTIGMSE EEAKIARARR EGTGLVAEIG TGKEPCVALR ADIDALPIFE RTNVPFRSKV DGQMHACGHD VHTTMLLGAA ALLKQLEPHM EGTIRLIFQP AEEGGGGALM MREEGVLTMA PPVEFIFGMH VAPALPTGEL ATRKGAMMAA ATQFSINVKG RGGHGAVPHE TIDPSPGVAA IVQGLYAIVA RETSFTENTT GLISVTRIQG GTAFNVIPSE YFIGGTIRAL DMAMMRNLQA RVVELVENLA QAFRCQADVK YGSVSYVPLV NDPDATEFFI QTAAPASRSG RVGIADPTLG GEDFAFFLED VPGTFAVIGI GSGAEHQLGH VPTNIPLHNP NFAVDERVLN RGAAVHAFTA LRAFSFLASK KRNAGAAETR TTCEA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccccgcggc ggccacaaga aaatggcttc gaaaaccttt tggaatcgag gaatcatctt tttacgttca tattctttct ttttccttcc tctgctggcg gcagccgcta gttcacctgc aagtgacgac aaatatatca tcccggacgg cgagctccag aacctccttg cggtgccaac gaatctgagc tctctgtcga actctggatc tcttgtcgac ttctccacgc tctttgaagc agccagtgaa acttctttca atgcatggat cgtggctgtc cgtcgagctc ttcaccagtg gccagaaaca gcgtacaatg agtacaggac ctctgcactg attcacaagc ttcttaaggc gatgaacgtt cgggtcacaa caggatgggg cacgaataca attggcatga gcgaagaaga agcgaaaatt gcacgggcgc gacgggaagg aacgggactg gttgcggaaa tcggaacagg gaaggaacct tgtgttgcgc tgcgagctga tatcgacgct cttcctatct tcgaacggac taacgttcct ttccggtcca aagttgacgg gcaaatgcat gcatgtggcc acgacgttca taccacgatg cttctgggag cagcggcgct gcttaagcag ctggagcccc acatggaggg aacgattcga cttatcttcc aacctgccga ggaaggcgga ggcggggcgc tcatgatgag agaagagggt gttctgacta tggcaccacc cgtagaattc attttcggga tgcatgtcgc cccggcatta cccacagggg aattggcgac aagaaaaggt gccatgatgg ccgccgcaac acaattctca atcaatgtga agggacgagg tggccatggc gccgtgccgc atgaaaccat cgacccgagt ccaggcgtag cagcgatcgt tcagggatta tacgcaatcg tggcgcggga aacgtcgttc acggaaaaca ctaccggtct tatctctgtc acccggatcc agggaggaac agcattcaac gtgataccat ctgaatactt cattggagga acgatccggg ccttggacat ggcgatgatg cggaacctac aggctagagt tgtagaactc gtggagaatt tggcccaggc gttcagatgt caagctgacg tcaaatatgg gtccgtcagc tacgtacctc ttgtcaatga cccagacgcg acagagttct tcatccagac cgcggcaccg gcgtcgcgga gcggaagggt ggggatagcg gacccgacgc tgggaggaga agatttcgca tttttcctcg aagacgttcc cggtacattt gcagtgattg gcatcggcag tggagctgaa caccagctag gccatgtccc tacaaacatt cccctccaca accccaactt cgctgtcgat gaacgagtgt tgaaccgcgg agctgctgtg cacgcgttca ctgcactgag ggccttttcg ttcttggcta gcaagaaacg taatgctggg gccgcggaaa cacgtacgac ctgtgaagcc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa