SSGCID: Target Page

TogoA.18022.a

Prolyl endopeptidase, putative (EC 3.4.21.26)

CENTER ID:	TogoA.18022.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.18022.a.A2.GE33594	domain 1		1	594
TogoA.18022.a.A3.GE33595	domain 2		615	825

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_286120
RefSeq:	XP_002369249.1
UniProt:	B6KN26

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLTSWLSVRC CSLIHIGGGR TTRQSFSYMS HFMRLFCRRK LSFLTSFSGH SPTRSDIQVA PLRTESCVAA TRGARPHHFL PRATHCSSAS STMVAAGVSH GNRSLTYFPA RRDESVVERH FGETVADPYR WLENPDSEET KEFVEEQNNV SRPLYRTPIR EKFFAKLEQI YDYPKYGVPF REGDMWFHWY NTGLQNQHVL YGRKSLTDET AEVVLDPNSW TEDGTAAVGN VVFTDDGSLM AYTRSERGSD WKRIFVKRVT DGYLFPDVLK HVKFSSLAWS PDNRGFFYNR YDVDDEDQDD SGRANAQHDF QKAFYHKLGT DQKEDILILA FPDQPKWLSE ITVSWDHRFL IAGIAESCSP TNQVWIADLD TIRTSNAQGM FDFSKLKWNK IIENFDAGYE YLTNDGTEFV FLTNKDAPLY KLVKIDLAKP EEASWKTLIE ERTELLSSAD VVDGDKLVLH YFRDVTSQLE VRNLHTGKLL GEISLPLGSV KKISGERRFP HLLFSFTSFT TPSDIYQVNV TAAAKSGDWR PTLYRQTKLD GADLSQLEVR QEFFDGKDGT KIPMFIVHKK GIKRDGSNPA NLYGYGGFNI SITPFFSASW VVAMLNLGMI MAVANIRGGG EYGRAWYEAA IKTKKQVSYD DFQQAAEYLI AQKYTSPQQL AIEGGSNGGL LVGACINQRP DLYGAAIIHV GVLDLLRFHK FTIGHAWTSD YGNPENEEDF PAILAISPLH NIGKGRGKGK GHQYPAVLLL TGDHDDRVSP FHSLKYIAEL QHSVGSSPKQ TNPLVIRVDT NTGHGAGKPV KKTIEEAADV YGFLANALHI QWHEQ

NT Sequence

atgctgacca gttggctgag cgtacgatgt tgttctctca tccacattgg cggcggcagg actactagac aaagtttctc ctacatgagc cactttatga gacttttttg tcgccggaaa ctttccttcc tgacttcatt ctcaggtcac agtccaaccc ggagtgacat acaggttgcg ccactacgca ctgagtcttg cgtagcagca acgcgaggag cccgtccgca tcacttccta ccccgagcca ctcactgttc ttcggcctct agcacaatgg tggctgctgg agtttctcat ggaaaccgtt cgctgactta ctttccagcg agaagagacg aaagtgttgt tgaaaggcac ttcggcgaga ccgttgcgga tccctacaga tggctggaga atcctgacag cgaggagacg aaggaattcg ttgaggagca gaacaatgtc tcgcgacctc tgtaccgaac gccgattcgc gagaagttct ttgcaaagtt ggagcagatc tacgactacc caaagtatgg cgttccgttt cgcgaaggag acatgtggtt tcactggtac aacaccgggc tgcagaacca gcatgtgttg tacggtcgaa aatccctcac cgacgaaaca gccgaggttg tcttggaccc taattcctgg actgaggacg gcactgccgc cgtaggcaac gtggtgttca cagacgacgg ttctttgatg gcgtacaccc gaagcgagcg gggaagtgac tggaagcgaa tttttgtgaa gcgagtcaca gatggctacc tctttccaga tgtgctcaag cacgtcaagt tctcgtcttt ggcgtggtcg ccggacaatc gaggcttttt ctacaaccgc tacgacgtgg acgatgaaga ccaagacgac agcggcagag caaacgctca gcacgacttc caaaaggcct tctaccacaa gttgggaacg gatcaaaaag aggacattct cattcttgcc ttccccgacc aacccaagtg gctcagtgag atcaccgttt cgtgggatca ccggttcctc atcgccggga ttgccgagag ctgcagcccg acaaaccaag tgtggatcgc ggacttggac acgatccgaa cctccaatgc acaaggcatg tttgacttct cgaaactgaa atggaacaag attatcgaaa actttgacgc tggatacgaa tacctcacca acgacggaac cgagttcgtg ttcctcacaa acaaggatgc gccgctgtac aagctcgtga agatcgacct tgcaaagcca gaagaagcat cctggaagac gcttattgag gagaggactg agttgctgag ctccgcggat gtggtggacg gagataagtt ggttctgcac tacttccgtg acgtcacctc ccagctggaa gtccgaaatc tccacacagg gaagctgcta ggagagattt ctcttccact cgggagtgtg aaaaaaatct ctggcgaacg cagattccct cacttgctct tctccttcac atccttcacg acaccctccg acatctacca ggtcaacgtg actgctgcgg ccaagagcgg ggactggaga ccgacgctgt accgacagac caagcttgac ggagcggact tgagccagct ggaagttcgc caggagtttt tcgatggcaa ggacggcacc aagatcccga tgttcatcgt gcacaaaaaa ggaatcaaac gcgatggcag caaccccgct aacctttacg gatacggcgg attcaacatc tcaataacgc cgttcttcag cgcctcgtgg gtcgttgcga tgctcaactt gggcatgatc atggcagttg cgaatatccg cggcggaggc gaatacggga gggcctggta tgaggctgca atcaaaacga agaaacaggt cagctacgat gacttccagc aagctgctga atatctcatc gcacaaaagt acacttcgcc tcaacagctg gcgattgagg gaggctccaa cggaggtctc ttggttggcg catgcatcaa ccagcggcct gatctctatg gcgccgcaat tatccacgtc ggtgtcctcg atctcttgcg ctttcacaaa ttcaccatcg gccatgcctg gacaagtgac tacggtaacc cggaaaacga agaggacttc cctgccattc tcgcaatttc gccgttgcac aacatcggca agggtcgtgg aaaaggtaaa ggacaccaat atccggcagt gttactcctg acaggagacc acgatgatcg cgtttccccc ttccactcgc tcaagtacat cgcggaactc cagcactctg tcgggagctc tccgaagcaa accaatccgt tggtgattcg cgtcgacacg aacaccggcc atggagcagg gaagccagtg aagaagacca tcgaagaggc ggcggatgtg tacggcttct tggcaaatgc gttgcacatt cagtggcatg agcagtaa

Details for TogoA.18022.a.A2.GE33594

HARVESTED ON:	10/31/2011
SEQUENCED ON:	11/4/2011
EXPECTED MW:	70kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	86
PERCENT COVERAGE:	39

Validated AA Sequence

Validated NT Sequence

aannnttttg tnnnnnttaa gaaggagata taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg ctgaccagtt ggctgagcgt acgatgttgt tctctcatcc acattggcgg cggcaggact actagacaaa gtttctccta catgagccac tttatgagac ttttttgtcg ccggaaactt tccttcctga cttcattctc aggtcacagt ccaacccgga gtgacataca ggttgcgcca ctacgcactg agtcttgcgt agcagcaacg cgaggagccc gtccgcatca cttcctaccc cgagccactc actgttcttc ggcctctagc acaatggtgg ctgctggagt ttctcatgga aaccgttcgc tgacttactt tccagcgaga agagacgaaa gtgttgttga aaggcacttc ggcgagaccg ttgcggatcc ctacagatgg ctggagaatc ctgacagcga ggagacgaan gaattcgttg aggagcagaa caatgtctcg cgacctctgt accgaacgcc gattcgcgag aagttctttg caaagttgga gcagatctac gactacccaa agtatggcgt tccgtttncg cgaangagac atgtggtttc actggtacaa caccgggctg cagaaccagc atgtgttgta cngtcnaaaa tccctnancg acgaaacagc cgangttgtc ntngnnccta nnncctnnnc tganganggn actncnnccg tgnnnntnnn ntcnnnnacn angnncnttg nngngtncan ncnanncnag cngggnannn actngannnn nttnnnnnnn nnnnnnnnnn gntncnnntt nnnnnnnnnn annnnnnagt nnnnnnnnnn nnnnnnnnnn ggnnannnng nnttnnnnnn nnnnnnnnnn gnnnannnnn nnnnnnnnnn nnnnannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLTSWLSVR CCSLIHIGGG RTTRQSFSYM SHFMRLFCRR KLSFLTSFSG HSPTRSDIQV APLRTESCVA ATRGARPHHF LPRATHCSSA SSTMVAAGVS HGNRSLTYFP ARRDESVVER HFGETVADPY RWLENPDSEE TKEFVEEQNN VSRPLYRTPI REKFFAKLEQ IYDYPKYGVP FREGDMWFHW YNTGLQNQHV LYGRKSLTDE TAEVVLDPNS WTEDGTAAVG NVVFTDDGSL MAYTRSERGS DWKRIFVKRV TDGYLFPDVL KHVKFSSLAW SPDNRGFFYN RYDVDDEDQD DSGRANAQHD FQKAFYHKLG TDQKEDILIL AFPDQPKWLS EITVSWDHRF LIAGIAESCS PTNQVWIADL DTIRTSNAQG MFDFSKLKWN KIIENFDAGY EYLTNDGTEF VFLTNKDAPL YKLVKIDLAK PEEASWKTLI EERTELLSSA DVVDGDKLVL HYFRDVTSQL EVRNLHTGKL LGEISLPLGS VKKISGERRF PHLLFSFTSF TTPSDIYQVN VTAAAKSGDW RPTLYRQTKL DGADLSQLEV RQEFFDGKDG TKIPMFIVHK KGIKRDGSNP ANLYGYGGFN ISITP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgaccagt tggctgagcg tacgatgttg ttctctcatc cacattggcg gcggcaggac tactagacaa agtttctcct acatgagcca ctttatgaga cttttttgtc gccggaaact ttccttcctg acttcattct caggtcacag tccaacccgg agtgacatac aggttgcgcc actacgcact gagtcttgcg tagcagcaac gcgaggagcc cgtccgcatc acttcctacc ccgagccact cactgttctt cggcctctag cacaatggtg gctgctggag tttctcatgg aaaccgttcg ctgacttact ttccagcgag aagagacgaa agtgttgttg aaaggcactt cggcgagacc gttgcggatc cctacagatg gctggagaat cctgacagcg aggagacgaa ggaattcgtt gaggagcaga acaatgtctc gcgacctctg taccgaacgc cgattcgcga gaagttcttt gcaaagttgg agcagatcta cgactaccca aagtatggcg ttccgtttcg cgaaggagac atgtggtttc actggtacaa caccgggctg cagaaccagc atgtgttgta cggtcgaaaa tccctcaccg acgaaacagc cgaggttgtc ttggacccta attcctggac tgaggacggc actgccgccg taggcaacgt ggtgttcaca gacgacggtt ctttgatggc gtacacccga agcgagcggg gaagtgactg gaagcgaatt tttgtgaagc gagtcacaga tggctacctc tttccagatg tgctcaagca cgtcaagttc tcgtctttgg cgtggtcgcc ggacaatcga ggctttttct acaaccgcta cgacgtggac gatgaagacc aagacgacag cggcagagca aacgctcagc acgacttcca aaaggccttc taccacaagt tgggaacgga tcaaaaagag gacattctca ttcttgcctt ccccgaccaa cccaagtggc tcagtgagat caccgtttcg tgggatcacc ggttcctcat cgccgggatt gccgagagct gcagcccgac aaaccaagtg tggatcgcgg acttggacac gatccgaacc tccaatgcac aaggcatgtt tgacttctcg aaactgaaat ggaacaagat tatcgaaaac tttgacgctg gatacgaata cctcaccaac gacggaaccg agttcgtgtt cctcacaaac aaggatgcgc cgctgtacaa gctcgtgaag atcgaccttg caaagccaga agaagcatcc tggaagacgc ttattgagga gaggactgag ttgctgagct ccgcggatgt ggtggacgga gataagttgg ttctgcacta cttccgtgac gtcacctccc agctggaagt ccgaaatctc cacacaggga agctgctagg agagatttct cttccactcg ggagtgtgaa aaaaatctct ggcgaacgca gattccctca cttgctcttc tccttcacat ccttcacgac accctccgac atctaccagg tcaacgtgac tgctgcggcc aagagcgggg actggagacc gacgctgtac cgacagacca agcttgacgg agcggacttg agccagctgg aagttcgcca ggagtttttc gatggcaagg acggcaccaa gatcccgatg ttcatcgtgc acaaaaaagg aatcaaacgc gatggcagca accccgctaa cctttacgga tacggcggat tcaacatctc aataacgccg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for TogoA.18022.a.A3.GE33595

HARVESTED ON:	10/31/2011
SEQUENCED ON:	11/4/2011
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNIRGGGEY GRAWYEAAIK TKKQVSYDDF QQAAEYLIAQ KYTSPQQLAI EGGSNGGLLV GACINQRPDL YGAAIIHVGV LDLLRFHKFT IGHAWTSDYG NPENEEDFPA ILAISPLHNI GKGRGKGKGH QYPAVLLLTG DHDDRVSPFH SLKYIAELQH SVGSSPKQTN PLVIRVDTNT GHGAXKPVKK TIEEAAXVYG FLANALHIQW HEQ

Validated NT Sequence

attttgtttn nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgaat atccgcggcg gaggcgaata cgggagggcc tggtatgagg ctgcaatcaa aacgaagaaa caggtcagct acgatgactt ccagcaagct gctgaatatc tcatcgcaca aaagtacact tcgcctcaac agctggcgat tgagggaggc tccaacggag gtctcttggt tggcgcatgc atcaaccagc ggcctgatct ctatggcgcc gcaattatcc acgtcggtgt cctcgatctc ttgcgctttc acaaattcac catcggccat gcctggacaa gtgactacgg taacccggaa aacgaagagg acttccctgc cattctcgca atttcgccgt tgcacaacat cggcaagggt cgtggaaaag gtaaaggaca ccaatatccg gcagtgttac tcctgacagg agaccacgat gatcgcgttt cccccttcca ctcgctcaag tacatcgcgg aactccagca ctctgtcggg agctctccga agcaaaccaa tccgttggtg attcgcgtcg acacgaacac cggccatgga gcanggaagc cagtgaagaa gaccatcgaa gaggcggcgg angtgtacgg cttcttggca aatgcgttgc acattcagtg gcatgagcag taataaacag cacgaacaag ttctgcagcc nagcttctcg aggatnccgg ctgctaacaa agcccgaang nagctgantt ggctgctgca cnnnnnnnnn nnnnnnnnnn nnnaanaant tnccccccnn nnnnnngngn nnnnnccnna angnnnnnnn annnnnaaan tnnnnnnnnn nnnnnnnnnn ntnnnnncnn nnnngggnnn nnntnnnnnn gnnnnnncnn nnnnnnnnnn gnannnnnnn nnnnnttnnn ngnnnnnnan nnnnncnnnn nnnnnnnnnn nnnnnnnnan nt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatatccgc ggcggaggcg aatacgggag ggcctggtat gaggctgcaa tcaaaacgaa gaaacaggtc agctacgatg acttccagca agctgctgaa tatctcatcg cacaaaagta cacttcgcct caacagctgg cgattgaggg aggctccaac ggaggtctct tggttggcgc atgcatcaac cagcggcctg atctctatgg cgccgcaatt atccacgtcg gtgtcctcga tctcttgcgc tttcacaaat tcaccatcgg ccatgcctgg acaagtgact acggtaaccc ggaaaacgaa gaggacttcc ctgccattct cgcaatttcg ccgttgcaca acatcggcaa gggtcgtgga aaaggtaaag gacaccaata tccggcagtg ttactcctga caggagacca cgatgatcgc gtttccccct tccactcgct caagtacatc gcggaactcc agcactctgt cgggagctct ccgaagcaaa ccaatccgtt ggtgattcgc gtcgacacga acaccggcca tggagcaggg aagccagtga agaagaccat cgaagaggcg gcggatgtgt acggcttctt ggcaaatgcg ttgcacattc agtggcatga gcagtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa