SSGCID: Target Page

TogoA.18017.a

M16 family peptidase, putative (EC 3.4.24.56)

CENTER ID:	TogoA.18017.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.18017.a.A2.GE33601	domain 1		16	665

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_269885
RefSeq:	XP_002365667.1
UniProt:	B6KCU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MWSPRAVTVS LSWVILSLLS LLFPVRKPRN DSRNYRYIEL PNELRALLVS DPECDEAAAS MRVGVGSMSD PPKIPGLAHF TEHMLFQGSK RFPGTHDFFD FVHNHGGYTN AFTSKFSTVF SFSIGPGFLE PGLDRLADLF SAPLLKSENL LKEVNAVHSE YIIDLTDDGR RKHHLIRQTA KGGPFSNFTV GNLESLMERT KQQGIDPVKA MREFHNKWYS SNLMTLAVVG RESLDVLESH VRKHFGNVPN GRVTPPVFEE CSEAFIPLDP NELGTETLVV PEADLHDATF VFYLPPQAKN WRSKPLQFIS EMLEHEGPTS LSSKLKREGL ITSLVTDYWS PELCTVLQVN VRLTEGGRSK ESVYKIGHAL FTFLRNLGVS RPERWRVTEM AKIRQLGFAF ADMPDPYALT VRAVEGLNYY TPEEVIAGDR LIYHFDPDII QQYVQKFLVP DNVRLFIFDK KLAADVDREE YWFKIKHGVE PIMDSAFKKW KEITSAPANT VQSMMRMEGM ALPAPNRFLP NNVAILPRPP GSGQGAEKSA FPEPLVFAGD HICANKCAVF HKQDTTFHSP KAVVELQIYY TGSHEDGVRE RILTALYVQS LRFALRERFA DTHRAGMSLG LGSGVAVGST TLPTVKRQRV LTLSAAGFSD KLDYVLRAFA KSLAATEGAS DEKPIPSPME LGVTMSHGVR HLMHSRVTAP SNVLASVQSS RVYTTAVRLP GGGRAARRTL THDQITTMHR KPASMDVTLM RTPVSSLGIR IVGGRAEREA TSVVAGGGKP LLEKRFFRLA FDQLRTDLRV ATLNRTPLAQ ANDAVLELIM HPHVPVHKMY AALFEMEKNH PTLDAIFEEV ADWGVKLWNE AAVEGLVQVR ISCSRGCALQ SFCRHMNGSG QVGHSCRKQW FSSVCVPCIS NRLRGLQPLL CARLVLFQLP PDVNVPCLFV G

NT Sequence

atgtggagtc caagagcagt cactgtttca ctgtcatggg tcatcctgtc tctgctgtca cttctgtttc cagttcggaa accacgaaac gacagtagaa actaccgcta catcgagctg ccaaacgagt tgcgagccct cctcgtttca gatccggaat gcgatgaagc agctgcatcg atgcgtgtgg gtgtgggctc catgtcggac ccgcccaaga tacctggcct ggcccatttt acagaacaca tgctgtttca gggatccaag cggttccccg gtacgcatga cttcttcgat ttcgtccaca accatggtgg ctataccaat gccttcacga gcaagttttc gacggtcttc tccttctcca ttggccccgg gttcctggaa cctgggctcg accgcttggc tgacctcttc tctgccccgc tgctgaaaag cgaaaacctg ctcaaagaag tcaacgccgt tcattcggag tacatcattg acctcaccga tgacggccgt aggaagcacc atctcattcg gcagacagct aaaggcggtc cgttctctaa tttcaccgtc ggaaacctcg agagcctcat ggagcggacg aagcagcagg gcatcgaccc ggtcaaagcc atgcgcgagt tccacaacaa atggtactcg tcaaatctca tgacgttggc tgtggtgggc cgagaatctc ttgatgtcct cgaaagtcac gtccgcaagc acttcggcaa cgtccccaat ggtcgtgtca cccctccagt attcgaagaa tgttctgaag cattcattcc tcttgatccg aatgaactcg gtactgaaac gctcgtagtt ccggaagcag accttcacga tgccactttc gtcttttatc tgccacccca agcgaagaac tggcgaagca agcctctgca gttcatttct gaaatgctgg aacacgaagg accaacatct ctgtcaagca aactcaaacg cgagggtctc atcacgtctc ttgttacgga ttactggtcg ccagagcttt gcaccgtctt acaggtcaac gtgcgcttaa cagaaggagg acgatccaag gagagcgtct ataaaatagg ccacgctctt ttcacctttc ttcgaaacct cggcgtctct cgccctgaac gctggcgtgt gacggagatg gccaagatcc gccagcttgg attcgcgttt gccgacatgc cggatccgta tgcattaact gttagagccg tggaagggct gaactactac acgcctgagg aagtgattgc tggagatcgg cttatctacc atttcgaccc ggacatcatt cagcagtatg tgcagaagtt cctggttccc gataacgttc gtcttttcat atttgataag aaacttgcgg cagatgtgga tcgcgaggag tactggttca agatcaagca tggagttgaa cccataatgg attccgcgtt caagaagtgg aaggaaatca cctcagcccc agctaacacg gtgcagagta tgatgcgaat ggaaggcatg gcacttcctg ctccaaatcg attccttcca aataatgtcg ccatccttcc tcgtccgcct ggttccgggc aaggcgctga gaagagtgcc ttcccggagc ctcttgtctt tgccggcgac cacatctgcg ccaacaaatg tgctgttttt cacaagcaag acaccacttt tcactctcca aaggctgtcg tcgagcttca aatatattat actggaagtc atgaagacgg cgtccgcgag cgcatcctga ctgcgctgta cgtgcagagt cttagattcg ctctgcgtga gcggttcgcc gatacgcaca gggcaggcat gtctcttggc ctgggtagcg gtgtcgccgt cgggtccacc acgcttccga ccgtaaagcg acagagagtg ctgacactct ctgcggcagg cttttcggac aagctcgatt atgtgcttcg cgcattcgca aagtctctcg cggcaactga gggtgcgtca gatgaaaagc caatcccttc acctatggaa cttggagtca ctatgtctca cggcgtgaga catttgatgc actcccgcgt caccgctcca tctaatgtac tcgcttcggt ccagtcaagc cgtgtataca ccaccgcagt tcgcttgcct ggaggcggcc gagccgcgcg tcggaccctg acacacgacc agatcacaac tatgcatcgg aaaccggcct cgatggatgt aactctgatg agaactcccg tatcctcatt gggcatccgg atcgtcggcg gaagagcaga aagagaagct acatcggttg ttgctggtgg aggaaaaccc ctcctggaga agcgtttctt caggctggcc tttgatcagc tgcggacaga cttgcgagta gcaaccctca accgcacccc tttagcgcaa gcgaacgacg ctgttttgga gctgatcatg cacccacatg ttccagtcca caaaatgtat gccgccttat tcgaaatgga aaagaatcac ccgactctgg acgcaatctt cgaagaggtt gcagactggg gggtaaagct ttggaatgaa gctgccgtcg agggacttgt ccaggtacgt atctcatgtt ctcgaggatg cgcgctgcag agcttttgcc ggcacatgaa cgggtcaggt caggttggtc actcctgcag aaaacagtgg ttttcttcag tttgtgttcc ctgtatctcc aaccggttac gtggtttgca gcctcttctg tgtgcaagac tcgtgttatt ccaattgcct ccagacgtga atgtgccttg tctctttgta gggtaa

Details for TogoA.18017.a.A2.GE33601

HARVESTED ON:	10/31/2011
SEQUENCED ON:	11/4/2011
EXPECTED MW:	75kDa
OBSERVED MW:	75kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	76
PERCENT COVERAGE:	56

Validated AA Sequence

Validated NT Sequence

ttgtttanct ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgctgtct ctgctgtcac ttctgtttcc agttcggaaa ccacgaaacg acagtagaaa ctaccgctac atcgagctgc caaacgagtt gcgagccctc ctcgtttcag atccggaatg cgatgaagca gctgcatcga tgcgtgtggg tgtgggctcc atgtcggacc cgcccaagat acctggcctg gcccatttta cagaacacat gctgtttcag ggatccaagc ggttccccgg tacgcatgac ttcttcgatt tcgtccacaa ccatggtggc tataccaatg ccttcacgag caagttttcg acggtcttct ccttctccat tggccccggg ttcctggaac ctgggctcga ccgcttggct gacctcttct ctgccccgct gctgaaaagc gaaaacctgc tcaaagaagt caacgccgtt cattcggagt acatcattga cctcaccgat gacggccgta ggaagcacca tctcattcgg cagacagcta aaggcggtcc gttctctaat ttcaccgtcg gaaacctcga gagcctcatg gagcggacga agcagcangg catcgacccg gtcaaagcca tgcgcgagtt ccacaacaaa tggtactcgt caaatctcat gacgttggct gtggtgggcc gagaatctct tgatgtcctc naaagtcacg tccgcaagca cttcggcanc gtccccnatg gncgtgtcac ccctncagta ttcgaagaat gttctgaagc attcattcct cttgatccga atgaantcgg tactgaaacg ctcgtagttn cgnaagcnga nnntcacgat gccactttcg tcntttatct gnnnccncna gnngaannan tggcnaagca nnnntgcann nnntnnngaa nngctnnann cnnnnnnnnn nnnnnnncan naannnaann cnnngntcnn nnnnnnnnnn cnnatnntgg nnnnnnnncn tnnnncnnnn tgntnannnn nnnnnnnnnn nannnnnnnn nannnnnnna nnnnnnnnnn annnnnnnnn nnnnntnnnn nnnnnnnnnn gnnnnnnnnn ncnnnnnnnn nnnnnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLSLLSLLF PVRKPRNDSR NYRYIELPNE LRALLVSDPE CDEAAASMRV GVGSMSDPPK IPGLAHFTEH MLFQGSKRFP GTHDFFDFVH NHGGYTNAFT SKFSTVFSFS IGPGFLEPGL DRLADLFSAP LLKSENLLKE VNAVHSEYII DLTDDGRRKH HLIRQTAKGG PFSNFTVGNL ESLMERTKQQ GIDPVKAMRE FHNKWYSSNL MTLAVVGRES LDVLESHVRK HFGNVPNGRV TPPVFEECSE AFIPLDPNEL GTETLVVPEA DLHDATFVFY LPPQAKNWRS KPLQFISEML EHEGPTSLSS KLKREGLITS LVTDYWSPEL CTVLQVNVRL TEGGRSKESV YKIGHALFTF LRNLGVSRPE RWRVTEMAKI RQLGFAFADM PDPYALTVRA VEGLNYYTPE EVIAGDRLIY HFDPDIIQQY VQKFLVPDNV RLFIFDKKLA ADVDREEYWF KIKHGVEPIM DSAFKKWKEI TSAPANTVQS MMRMEGMALP APNRFLPNNV AILPRPPGSG QGAEKSAFPE PLVFAGDHIC ANKCAVFHKQ DTTFHSPKAV VELQIYYTGS HEDGVRERIL TALYVQSLRF ALRERFADTH RAGMSLGLGS GVAVGSTTLP TVKRQRVLTL SAAGFSDKLD YVLRAFAKSL AA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgtctctg ctgtcacttc tgtttccagt tcggaaacca cgaaacgaca gtagaaacta ccgctacatc gagctgccaa acgagttgcg agccctcctc gtttcagatc cggaatgcga tgaagcagct gcatcgatgc gtgtgggtgt gggctccatg tcggacccgc ccaagatacc tggcctggcc cattttacag aacacatgct gtttcaggga tccaagcggt tccccggtac gcatgacttc ttcgatttcg tccacaacca tggtggctat accaatgcct tcacgagcaa gttttcgacg gtcttctcct tctccattgg ccccgggttc ctggaacctg ggctcgaccg cttggctgac ctcttctctg ccccgctgct gaaaagcgaa aacctgctca aagaagtcaa cgccgttcat tcggagtaca tcattgacct caccgatgac ggccgtagga agcaccatct cattcggcag acagctaaag gcggtccgtt ctctaatttc accgtcggaa acctcgagag cctcatggag cggacgaagc agcagggcat cgacccggtc aaagccatgc gcgagttcca caacaaatgg tactcgtcaa atctcatgac gttggctgtg gtgggccgag aatctcttga tgtcctcgaa agtcacgtcc gcaagcactt cggcaacgtc cccaatggtc gtgtcacccc tccagtattc gaagaatgtt ctgaagcatt cattcctctt gatccgaatg aactcggtac tgaaacgctc gtagttccgg aagcagacct tcacgatgcc actttcgtct tttatctgcc accccaagcg aagaactggc gaagcaagcc tctgcagttc atttctgaaa tgctggaaca cgaaggacca acatctctgt caagcaaact caaacgcgag ggtctcatca cgtctcttgt tacggattac tggtcgccag agctttgcac cgtcttacag gtcaacgtgc gcttaacaga aggaggacga tccaaggaga gcgtctataa aataggccac gctcttttca cctttcttcg aaacctcggc gtctctcgcc ctgaacgctg gcgtgtgacg gagatggcca agatccgcca gcttggattc gcgtttgccg acatgccgga tccgtatgca ttaactgtta gagccgtgga agggctgaac tactacacgc ctgaggaagt gattgctgga gatcggctta tctaccattt cgacccggac atcattcagc agtatgtgca gaagttcctg gttcccgata acgttcgtct tttcatattt gataagaaac ttgcggcaga tgtggatcgc gaggagtact ggttcaagat caagcatgga gttgaaccca taatggattc cgcgttcaag aagtggaagg aaatcacctc agccccagct aacacggtgc agagtatgat gcgaatggaa ggcatggcac ttcctgctcc aaatcgattc cttccaaata atgtcgccat ccttcctcgt ccgcctggtt ccgggcaagg cgctgagaag agtgccttcc cggagcctct tgtctttgcc ggcgaccaca tctgcgccaa caaatgtgct gtttttcaca agcaagacac cacttttcac tctccaaagg ctgtcgtcga gcttcaaata tattatactg gaagtcatga agacggcgtc cgcgagcgca tcctgactgc gctgtacgtg cagagtctta gattcgctct gcgtgagcgg ttcgccgata cgcacagggc aggcatgtct cttggcctgg gtagcggtgt cgccgtcggg tccaccacgc ttccgaccgt aaagcgacag agagtgctga cactctctgc ggcaggcttt tcggacaagc tcgattatgt gcttcgcgca ttcgcaaagt ctctcgcggc ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa