TogoA.17897.a

FtsH protease, putative

CENTER ID: TogoA.17897.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.17897.a.A3.GE33587 domain 2 293 902
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.17897.a.A3.PS01420 domain 2 293 902

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_300020
RefSeq: XP_002371725.1
UniProt: B6KV52

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRSLLAQAPL GPRGTPAQAA ALQGTLSTVA TPAALLRRPF ATLPPPRFAA SFFSPSASVS PASSATASTL KHLQEQALRH PSDPACWMRY FQALNARGDS REVVRQFEEN LLVKQLSPEH LRGDAATASA LHGVVAEYLK ALCACDGIHS QALSALARDL FPLLSAGTST SPAKRAETTT APSASRDVES EMRLLQLNQL QLLLQQLQHL SSQLAQERTT GNAGALGRYG TSAAVEREQE AKSRARGVLG WTLFAVHGVL SGVWRTFKVG SAGLWSLLLS PIAAVLLIWS FATEALPDLQ QAAKKAEGLP EGKSKEWVQG KIVQPDQVKT TFNDVKGCEE VKKEVEEVVA YLKSPEKFTA MGARLPKGIL LQGPPGTGKT LLARAIAGEA GVPFLHASGS EFEEMFVGVG ASRLRQLFAE ARRLSPCILF IDEIDALGGK RTLTENKHHR QTLNQLLTEL DGFNPSDGVT LVCATNLLEA LDPALTRPGR IDRIIHVPFP SKKERIEILK HYAKEMPLAA DVDLEALAGL TSGMTGADLS NLLNFAAIRA ATEGKEQVTR AEVDESFDRL MVGSRRTGVV MKEEERRLTA YHESGHALVA LYTPASAPLH KATILFRGSS LGVTWSVEKE DTFSQSEQQC LASLDVAMGG KAAEELAFGA GKVTSGCRSD LVRATQLARA MVTNYGMGFT DSKAPMVIGR QDYLLVSDEK KSRVDEAVQK LLDESYARAR RLLEEKRDEL TRLAEALLEH ETLSAEEVRL AVAGKLRAKP RAEAQQTAEE AGEELHVSSS LSNNEDRKLE KRSAPGSAPT AEERENATDR SAHTRKETHK ETRGQVSPTT GPSAALAPSP YAVAAAVEVS ELLAERQRRR EQGGGGPEKE GKEREKKKSE PGAGEGTAAN GR
NT Sequence
atgcggtctc tgctggctca ggcgcccctc gggccgcgcg ggactcccgc gcaggctgcg gcgttgcagg ggacattgtc gactgtggct acgcccgctg cactccttcg gcgtccgttc gcgactctcc ctccgccgcg cttcgccgcc tccttcttct ccccctctgc gtctgtctcg cctgcctcgt ccgcgaccgc ttcgactctg aagcatttgc aggagcaggc gctgcggcat ccctcggatc ctgcatgttg gatgcgctac ttccaagcgc tgaacgctcg gggagactcg cgcgaagtcg tgagacagtt cgaagagaac ctgcttgtga agcagttgtc tccggagcac ctgcgcggcg acgcggcgac ggcttctgct ctccacggtg tcgtggcgga gtatctgaag gccctctgcg cctgcgacgg aattcactcg caagctctct ctgcgctcgc cagagacctc ttcccccttc tgtccgcggg gacctccacc tcgccagcga agcgcgcgga gacaacgact gcgccttccg cctccagaga tgtcgagagc gaaatgcgcc tcctccaact aaatcagctg cagctgctcc tgcagcagct gcaacatctg tcctcgcagc tcgcgcaaga gcgaaccacg ggcaacgcgg gcgcccttgg ccgctacggc acgagcgcgg cggtcgaaag agaacaagaa gcgaagtcgc gcgcgagagg cgtcctaggc tggactctct tcgctgtcca cggcgtactt agcggcgtct ggagaacctt caaagtcggc agcgcgggtc tatggagtct cctgctctct ccaatcgcgg cggttctgct gatttggagc ttcgcgacgg aggcgctgcc ggacttgcaa caggcagcga agaaggccga gggactccca gaaggaaaat ccaaggaatg ggtacaagga aaaatcgtgc agcctgacca agtgaaaacc actttcaatg atgtcaaggg gtgcgaagag gtcaagaagg aagtcgaaga ggttgtcgcc tatctcaaat ctccagagaa attcacggct atgggagcgc gcctgccgaa gggcattctc ctgcaaggtc ccccagggac tggcaaaaca ctgctggcca gggcgatcgc tggagaggca ggcgttccct tcctccacgc ctcaggctcg gaattcgagg agatgttcgt cggcgtcgga gcgagtcggc tgcgacagct ctttgcggag gctcggcgtc tgagtccctg cattttgttc atcgacgaaa tcgacgcctt ggggggaaaa cgaactttga cagaaaacaa gcatcatcgg caaacgctga atcaactcct gaccgaactc gacggattca atccttccga cggagtgact ctcgtttgcg ccacaaatct tctggaggct ctcgaccctg cgttgacgcg accgggccga atcgaccgaa ttattcatgt ccccttcccc tccaaaaaag aacgcatcga gattttgaaa cattatgcga aggagatgcc actcgcggca gacgtggact tggaggctct tgcgggtctg acgtctggga tgacaggagc agatttgtcg aatcttctga actttgctgc gatccgggca gcgactgaag gcaaagaaca agtgacgcgc gcggaagtag acgaaagttt cgatcgtttg atggtcggca gtcgccggac aggtgtggtg atgaaggagg aggagcgtcg cctcaccgcc taccacgagt ccgggcatgc actggtggcg ctgtacacgc cggcgagtgc gccgctgcac aaggcgacga tcttgtttcg cggctcgtcg ctgggtgtga cctggtcggt ggagaaggaa gatactttct cccagtcgga gcagcagtgc ctcgcgtctc tggacgtcgc gatgggaggc aaagctgcgg aggagctcgc cttcggcgca gggaaagtca cgagcggctg cagaagcgac ctggtccgcg ccacccagct cgcgagagcg atggtcacga actacggcat gggattcacc gactcgaagg cgccgatggt catcggcaga caagactacc tgctcgtgag cgacgagaag aaaagccgcg tcgacgaggc cgtccagaaa ctcctcgacg aaagctacgc gcgcgccagg cgcctgctcg aggagaaacg agacgaactc acgcggctgg ccgaggcact gttggagcat gagactttgt cagcggagga agttcgcctg gcggttgctg ggaaactgcg agcaaagcct cgcgcagagg ctcagcaaac tgctgaggag gcgggagagg agcttcacgt ttcttcttct ttgtcgaaca atgaagaccg gaagctagag aaaagatctg ctccagggtc ggcccccact gcagaggaga gagaaaatgc aacggacaga agtgcacaca ctcgaaagga gactcacaag gagacacgag gccaggtgtc tccgacgaca gggccatcgg ccgcgctcgc gccgtcgccc tacgcggtcg cggcggcagt tgaagtctcc gagctgcttg ccgagcgcca acgaaggcga gagcaaggcg gggggggtcc ggagaaagaa ggaaaagaaa gagagaaaaa gaaatccgag ccgggcgctg gagaaggaac agcggccaac ggcagatag
Details for TogoA.17897.a.A3.GE33587
HARVESTED ON: 10/31/2011
SEQUENCED ON: 11/4/2011
EXPECTED MW: 68kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 60
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTEALPDLQ QAAKKAEGLP EGKSKEWVQG KIVQPDQVKT TFNDVKGCEE VKKEVEEVVA YLKSPEKFTA MGARLPKGIL LQGPPGTGKT LLARAIAGEA GVPFLHASGS EFEEMFVGVG ASRLRQLFAE ARRLSPCILF IDEIDALGGK RTLTENKHHR QTLNQLLTEL DGFNPSDGVT LVCATNLLEA LDPALTRPGR IDRIIHVPFP SKKERIEILK HYAKEMPLAA DVDLXALAGL TSGMTGAXLS NLLNFAAIRA ATEGKEQVTR AEVDESFDRL MVGSRXTGVV MXXXXSXXXX XVXHXLXALY TXXXXXCXXX XXXXXXXLXX XXXXXXXXXX XXXXXXXXXX XXXXXXXLXX XX
Validated NT Sequence
ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacgga ggcgctgccg gacttgcaac aggcagcgaa gaaggccgag ggactcccag aaggaaaatc caaggaatgg gtacaaggaa aaatcgtgca gcctgaccaa gtgaaaacca ctttcaatga tgtcaagggg tgcgaagagg tcaagaagga agtcgaagag gttgtcgcct atctcaaatc tccagagaaa ttcacggcta tgggagcgcg cctgccgaag ggcattctcc tgcaaggtcc cccagggact ggcaaaacac tgctggccag ggcgatcgct ggagaggcag gcgttccctt cctccacgcc tcaggctcgg aattcgagga gatgttcgtc ggcgtcggag cgagtcggct gcgacagctc tttgcggagg ctcggcgtct gagtccctgc attttgttca tcgacgaaat cgacgccttg gggggaaaac gaactttgac agaaaacaag catcatcggc aaacgctgaa tcaactcctg accgaactcg acggattcaa tccttccgac ggagtgactc tcgtttgcgc cacaaatctt ctggaggctc tcgaccctgc gttgacgcga ccgggccgaa tcgaccgaat tattcatgtc cccttcccct ccaaaaaaga acgcatcgag attttgaaac attatgcgaa ggagatgcca ctcgcggcag acgtggactt ggangctctt gcgggtctga cgtctgggat gacaggagca ganttgtcga atcttctgaa ctttgctgcg atccgggcag cgactgaagg caaagaacaa gtgacgcgcg cggaagtaga cgaaagtttc gatcgtttga tggtcggcag tcgnnngaca ggtgtggtga tgannnnnnn ancgtcncnn nnncnacnac nagtcnggca tgnactggng gcgctgtaca cnnnncnann gngcngctgc nnnannnncn annnngnttn nnnnnnnncn ctggnnannn nnnnngnnng nnnnnnnnnn nnnnttnnnn ccnnnnnnnn nnnnntngnn nnnnnnnnnn ngnnnnnnnn nnnnnnnnnn cttnnnnnnn ngnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTEALPDLQ QAAKKAEGLP EGKSKEWVQG KIVQPDQVKT TFNDVKGCEE VKKEVEEVVA YLKSPEKFTA MGARLPKGIL LQGPPGTGKT LLARAIAGEA GVPFLHASGS EFEEMFVGVG ASRLRQLFAE ARRLSPCILF IDEIDALGGK RTLTENKHHR QTLNQLLTEL DGFNPSDGVT LVCATNLLEA LDPALTRPGR IDRIIHVPFP SKKERIEILK HYAKEMPLAA DVDLEALAGL TSGMTGADLS NLLNFAAIRA ATEGKEQVTR AEVDESFDRL MVGSRRTGVV MKEEERRLTA YHESGHALVA LYTPASAPLH KATILFRGSS LGVTWSVEKE DTFSQSEQQC LASLDVAMGG KAAEELAFGA GKVTSGCRSD LVRATQLARA MVTNYGMGFT DSKAPMVIGR QDYLLVSDEK KSRVDEAVQK LLDESYARAR RLLEEKRDEL TRLAEALLEH ETLSAEEVRL AVAGKLRAKP RAEAQQTAEE AGEELHVSSS LSNNEDRKLE KRSAPGSAPT AEERENATDR SAHTRKETHK ETRGQVSPTT GPSAALAPSP YAVAAAVEVS ELLAERQRRR EQGGGGPEKE GKEREKKKSE PGAGEGTAAN GR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggaggcg ctgccggact tgcaacaggc agcgaagaag gccgagggac tcccagaagg aaaatccaag gaatgggtac aaggaaaaat cgtgcagcct gaccaagtga aaaccacttt caatgatgtc aaggggtgcg aagaggtcaa gaaggaagtc gaagaggttg tcgcctatct caaatctcca gagaaattca cggctatggg agcgcgcctg ccgaagggca ttctcctgca aggtccccca gggactggca aaacactgct ggccagggcg atcgctggag aggcaggcgt tcccttcctc cacgcctcag gctcggaatt cgaggagatg ttcgtcggcg tcggagcgag tcggctgcga cagctctttg cggaggctcg gcgtctgagt ccctgcattt tgttcatcga cgaaatcgac gccttggggg gaaaacgaac tttgacagaa aacaagcatc atcggcaaac gctgaatcaa ctcctgaccg aactcgacgg attcaatcct tccgacggag tgactctcgt ttgcgccaca aatcttctgg aggctctcga ccctgcgttg acgcgaccgg gccgaatcga ccgaattatt catgtcccct tcccctccaa aaaagaacgc atcgagattt tgaaacatta tgcgaaggag atgccactcg cggcagacgt ggacttggag gctcttgcgg gtctgacgtc tgggatgaca ggagcagatt tgtcgaatct tctgaacttt gctgcgatcc gggcagcgac tgaaggcaaa gaacaagtga cgcgcgcgga agtagacgaa agtttcgatc gtttgatggt cggcagtcgc cggacaggtg tggtgatgaa ggaggaggag cgtcgcctca ccgcctacca cgagtccggg catgcactgg tggcgctgta cacgccggcg agtgcgccgc tgcacaaggc gacgatcttg tttcgcggct cgtcgctggg tgtgacctgg tcggtggaga aggaagatac tttctcccag tcggagcagc agtgcctcgc gtctctggac gtcgcgatgg gaggcaaagc tgcggaggag ctcgccttcg gcgcagggaa agtcacgagc ggctgcagaa gcgacctggt ccgcgccacc cagctcgcga gagcgatggt cacgaactac ggcatgggat tcaccgactc gaaggcgccg atggtcatcg gcagacaaga ctacctgctc gtgagcgacg agaagaaaag ccgcgtcgac gaggccgtcc agaaactcct cgacgaaagc tacgcgcgcg ccaggcgcct gctcgaggag aaacgagacg aactcacgcg gctggccgag gcactgttgg agcatgagac tttgtcagcg gaggaagttc gcctggcggt tgctgggaaa ctgcgagcaa agcctcgcgc agaggctcag caaactgctg aggaggcggg agaggagctt cacgtttctt cttctttgtc gaacaatgaa gaccggaagc tagagaaaag atctgctcca gggtcggccc ccactgcaga ggagagagaa aatgcaacgg acagaagtgc acacactcga aaggagactc acaaggagac acgaggccag gtgtctccga cgacagggcc atcggccgcg ctcgcgccgt cgccctacgc ggtcgcggcg gcagttgaag tctccgagct gcttgccgag cgccaacgaa ggcgagagca aggcgggggg ggtccggaga aagaaggaaa agaaagagag aaaaagaaat ccgagccggg cgctggagaa ggaacagcgg ccaacggcag ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for TogoA.17897.a.A3.PS01420
PURIFICATION DATe: 3/9/2012
CONCENTRATION: 45.68mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 82
PERCENT COVERAGE: 60
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTEALPDLQ QAAKKAEGLP EGKSKEWVQG KIVQPDQVKT TFNDVKGCEE VKKEVEEVVA YLKSPEKFTA MGARLPKGIL LQGPPGTGKT LLARAIAGEA GVPFLHASGS EFEEMFVGVG ASRLRQLFAE ARRLSPCILF IDEIDALGGK RTLTENKHHR QTLNQLLTEL DGFNPSDGVT LVCATNLLEA LDPALTRPGR IDRIIHVPFP SKKERIEILK HYAKEMPLAA DVDLXALAGL TSGMTGAXLS NLLNFAAIRA ATEGKEQVTR AEVDESFDRL MVGSRXTGVV MXXXXSXXXX XVXHXLXALY TXXXXXCXXX XXXXXXXLXX XXXXXXXXXX XXXXXXXXXX XXXXXXXLXX XX
Validated NT Sequence
ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacgga ggcgctgccg gacttgcaac aggcagcgaa gaaggccgag ggactcccag aaggaaaatc caaggaatgg gtacaaggaa aaatcgtgca gcctgaccaa gtgaaaacca ctttcaatga tgtcaagggg tgcgaagagg tcaagaagga agtcgaagag gttgtcgcct atctcaaatc tccagagaaa ttcacggcta tgggagcgcg cctgccgaag ggcattctcc tgcaaggtcc cccagggact ggcaaaacac tgctggccag ggcgatcgct ggagaggcag gcgttccctt cctccacgcc tcaggctcgg aattcgagga gatgttcgtc ggcgtcggag cgagtcggct gcgacagctc tttgcggagg ctcggcgtct gagtccctgc attttgttca tcgacgaaat cgacgccttg gggggaaaac gaactttgac agaaaacaag catcatcggc aaacgctgaa tcaactcctg accgaactcg acggattcaa tccttccgac ggagtgactc tcgtttgcgc cacaaatctt ctggaggctc tcgaccctgc gttgacgcga ccgggccgaa tcgaccgaat tattcatgtc cccttcccct ccaaaaaaga acgcatcgag attttgaaac attatgcgaa ggagatgcca ctcgcggcag acgtggactt ggangctctt gcgggtctga cgtctgggat gacaggagca ganttgtcga atcttctgaa ctttgctgcg atccgggcag cgactgaagg caaagaacaa gtgacgcgcg cggaagtaga cgaaagtttc gatcgtttga tggtcggcag tcgnnngaca ggtgtggtga tgannnnnnn ancgtcncnn nnncnacnac nagtcnggca tgnactggng gcgctgtaca cnnnncnann gngcngctgc nnnannnncn annnngnttn nnnnnnnncn ctggnnannn nnnnngnnng nnnnnnnnnn nnnnttnnnn ccnnnnnnnn nnnnntngnn nnnnnnnnnn ngnnnnnnnn nnnnnnnnnn cttnnnnnnn ngnnnnt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTEALPDLQ QAAKKAEGLP EGKSKEWVQG KIVQPDQVKT TFNDVKGCEE VKKEVEEVVA YLKSPEKFTA MGARLPKGIL LQGPPGTGKT LLARAIAGEA GVPFLHASGS EFEEMFVGVG ASRLRQLFAE ARRLSPCILF IDEIDALGGK RTLTENKHHR QTLNQLLTEL DGFNPSDGVT LVCATNLLEA LDPALTRPGR IDRIIHVPFP SKKERIEILK HYAKEMPLAA DVDLEALAGL TSGMTGADLS NLLNFAAIRA ATEGKEQVTR AEVDESFDRL MVGSRRTGVV MKEEERRLTA YHESGHALVA LYTPASAPLH KATILFRGSS LGVTWSVEKE DTFSQSEQQC LASLDVAMGG KAAEELAFGA GKVTSGCRSD LVRATQLARA MVTNYGMGFT DSKAPMVIGR QDYLLVSDEK KSRVDEAVQK LLDESYARAR RLLEEKRDEL TRLAEALLEH ETLSAEEVRL AVAGKLRAKP RAEAQQTAEE AGEELHVSSS LSNNEDRKLE KRSAPGSAPT AEERENATDR SAHTRKETHK ETRGQVSPTT GPSAALAPSP YAVAAAVEVS ELLAERQRRR EQGGGGPEKE GKEREKKKSE PGAGEGTAAN GR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGAGGCG CTGCCGGACT TGCAACAGGC AGCGAAGAAG GCCGAGGGAC TCCCAGAAGG AAAATCCAAG GAATGGGTAC AAGGAAAAAT CGTGCAGCCT GACCAAGTGA AAACCACTTT CAATGATGTC AAGGGGTGCG AAGAGGTCAA GAAGGAAGTC GAAGAGGTTG TCGCCTATCT CAAATCTCCA GAGAAATTCA CGGCTATGGG AGCGCGCCTG CCGAAGGGCA TTCTCCTGCA AGGTCCCCCA GGGACTGGCA AAACACTGCT GGCCAGGGCG ATCGCTGGAG AGGCAGGCGT TCCCTTCCTC CACGCCTCAG GCTCGGAATT CGAGGAGATG TTCGTCGGCG TCGGAGCGAG TCGGCTGCGA CAGCTCTTTG CGGAGGCTCG GCGTCTGAGT CCCTGCATTT TGTTCATCGA CGAAATCGAC GCCTTGGGGG GAAAACGAAC TTTGACAGAA AACAAGCATC ATCGGCAAAC GCTGAATCAA CTCCTGACCG AACTCGACGG ATTCAATCCT TCCGACGGAG TGACTCTCGT TTGCGCCACA AATCTTCTGG AGGCTCTCGA CCCTGCGTTG ACGCGACCGG GCCGAATCGA CCGAATTATT CATGTCCCCT TCCCCTCCAA AAAAGAACGC ATCGAGATTT TGAAACATTA TGCGAAGGAG ATGCCACTCG CGGCAGACGT GGACTTGGAG GCTCTTGCGG GTCTGACGTC TGGGATGACA GGAGCAGATT TGTCGAATCT TCTGAACTTT GCTGCGATCC GGGCAGCGAC TGAAGGCAAA GAACAAGTGA CGCGCGCGGA AGTAGACGAA AGTTTCGATC GTTTGATGGT CGGCAGTCGC CGGACAGGTG TGGTGATGAA GGAGGAGGAG CGTCGCCTCA CCGCCTACCA CGAGTCCGGG CATGCACTGG TGGCGCTGTA CACGCCGGCG AGTGCGCCGC TGCACAAGGC GACGATCTTG TTTCGCGGCT CGTCGCTGGG TGTGACCTGG TCGGTGGAGA AGGAAGATAC TTTCTCCCAG TCGGAGCAGC AGTGCCTCGC GTCTCTGGAC GTCGCGATGG GAGGCAAAGC TGCGGAGGAG CTCGCCTTCG GCGCAGGGAA AGTCACGAGC GGCTGCAGAA GCGACCTGGT CCGCGCCACC CAGCTCGCGA GAGCGATGGT CACGAACTAC GGCATGGGAT TCACCGACTC GAAGGCGCCG ATGGTCATCG GCAGACAAGA CTACCTGCTC GTGAGCGACG AGAAGAAAAG CCGCGTCGAC GAGGCCGTCC AGAAACTCCT CGACGAAAGC TACGCGCGCG CCAGGCGCCT GCTCGAGGAG AAACGAGACG AACTCACGCG GCTGGCCGAG GCACTGTTGG AGCATGAGAC TTTGTCAGCG GAGGAAGTTC GCCTGGCGGT TGCTGGGAAA CTGCGAGCAA AGCCTCGCGC AGAGGCTCAG CAAACTGCTG AGGAGGCGGG AGAGGAGCTT CACGTTTCTT CTTCTTTGTC GAACAATGAA GACCGGAAGC TAGAGAAAAG ATCTGCTCCA GGGTCGGCCC CCACTGCAGA GGAGAGAGAA AATGCAACGG ACAGAAGTGC ACACACTCGA AAGGAGACTC ACAAGGAGAC ACGAGGCCAG GTGTCTCCGA CGACAGGGCC ATCGGCCGCG CTCGCGCCGT CGCCCTACGC GGTCGCGGCG GCAGTTGAAG TCTCCGAGCT GCTTGCCGAG CGCCAACGAA GGCGAGAGCA AGGCGGGGGG GGTCCGGAGA AAGAAGGAAA AGAAAGAGAG AAAAAGAAAT CCGAGCCGGG CGCTGGAGAA GGAACAGCGG CCAACGGCAG ATAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA