TogoA.17789.a

Eukaryotic aspartyl protease, putative (EC 3.4.23.34)

CENTER ID: TogoA.17789.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.17789.a.A11.GE33759 full length 1 469
TogoA.17789.a.A12.GE33760 full length 1 450
TogoA.17789.a.A13.GE33761 full length 27 469
TogoA.17789.a.A14.GE33762 full length 27 450
TogoA.17789.a.A15.GE33763 full length 47 469
TogoA.17789.a.A16.GE33764 full length 47 450
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_262940
RefSeq: XP_002365394.1
UniProt: Q6PS96

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLFHTIACL AVCCCPAFAA KARSKHMVRE RVAEQEIFAQ TGSVEESLFH KPEFNVLLKN RYQETGLKDF ISRLLEHHGA LATGKHDAAQ KAHQELLNFH NSQYFGEIQV GTPPVSFIVV FDTGSSNLWI PASECKQGGC VPHTRFDPKT SSTYLPINAG AGEPAIAFIQ YGTGACVLRM AKDTVSIGGI RVQNQTLGLA LQESVHPFAD LPFDGLVGLG FPDVAGEEGL PPDALPLVDS MMKQKLLKRN AFAVYMSEDL NQPGEITFGS VNPRHTFEGH KPQWFPVISL DYWEVGVHGL RLNRKSLGFC ERTRCKAAVD TGSSLITGPS KVINPLLKSL NVAEDCSNKG NLPTVTFILE DTAGRLVKFP LKPSDYLVEE VDSQGRLVTC VAGFIPMDVP APRGPLFVLG NSFIRKFYTI FDRDHMMVGF MRANHNGSGP SMKESSGSST SIYWAALLGV MSLCVRATV
NT Sequence
atgaggctgt ttcacacgat tgcatgcttg gctgtctgct gctgcccagc gtttgcggca aaggcacgat cgaaacacat ggttcgcgaa cgtgtagcag aacaagagat tttcgcacaa acggggtccg tagaggaatc tctgttccac aaacccgaat tcaatgtgct gctgaaaaat cgttaccaag aaaccggtct taaggatttt atatctcgtc ttctcgagca tcatggggcc ctggcgaccg gcaaacacga tgctgcccag aaggcccacc aggagctact caactttcat aacagtcaat acttcggaga aatccaagtt gggacgcctc ctgtttcgtt tattgtggtt tttgataccg gatcctcaaa cctctggata cccgcatctg agtgcaaaca aggtggttgt gtgccgcata cccgctttga tccaaaaact tcctctactt acctaccgat aaatgctggt gctggggaac cagccatcgc ctttatccag tacgggacag gtgcatgtgt tcttcggatg gcgaaagata cggtgtccat tggagggata cgagtgcaaa atcaaaccct cggcttagcc cttcaggagt ccgtgcatcc gttcgccgat ctgccgttcg atggcctcgt tggcctcggt ttccctgatg tcgcaggaga agaaggcctt ccgcccgacg ctcttcccct cgtggactca atgatgaagc aaaaactact taaaagaaat gcgttcgccg tatatatgag tgaagatttg aatcagcctg gagaaataac gttcggctcc gtaaaccctc gacatacgtt cgaaggccac aaaccccagt ggttccccgt gatatctctg gactactggg aagttggagt tcatggccta cgcctcaata gaaagtctct aggcttttgc gaacgcactc ggtgcaaagc ggccgtggac acgggttcaa gcttgatcac cggcccctcg aaagtgatta accctctcct aaaatcgcta aacgtggctg aagactgttc aaacaaaggc aatctcccaa cggtcacatt tattcttgag gacactgccg gacgtctcgt taagttccca ctgaaaccat ctgactatct agtcgaggaa gtcgactctc aaggaagatt ggtcacttgc gtagctggct tcataccaat ggatgttcct gctcctcgtg gtcctctttt cgttcttggg aattcgttta tcaggaagtt ctacacaatt tttgatcggg atcacatgat ggttggattc atgagagcca accacaacgg aagtggccca tcaatgaaag aatcatccgg gagctcaacc agcatctatt gggcagcact gctcggcgtt atgagcttgt gcgtcagagc gaccgtttga
Details for TogoA.17789.a.A11.GE33759
HARVESTED ON: 10/4/2011
SEQUENCED ON: 10/7/2011
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLFHTIAC LAVCCCPAFA AKARSKHMVR ERVAEQEIFA QTGSVEESLF HKPEFNVLLK NRYQETGLKD FISRLLEHHG ALATGKHDAA QKAHQELLNF HNSQYFGEIQ VGTPPVSFIV VFDTGSSNLW IPASECKQGG CVPHTRFDPK TSSTYLPINA GAGEPAIAFI QYGTGACVLR MAKDTVSIGG IRVQNQTLGL ALQESVHPFA DLPFDGLVGL GFPDVAGEEG LPPDALPLVD SMMKQKLLKR NAFAVYMSED LNQPGEITFG SVNPRHTFEG HKPQWFPVIS LDYWEVGVHG LRLNRKSLGF CERTRCKAAV DTGSSLITGP SKVINPLLKS LNVAEDCSNK GNLPTVTFIL EDTAGRLVKF PLKPSDYLVE EVDSQGRLVT CVAGFIPMDV PAPRGPLFVL GNSFIRKFYT IFDRDHMMVG FMRANHNGSG PSMKESSGSS TSIYWAALLG VMSLCVRATV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtctgttc cacaccatcg cttgcctcgc agtgtgctgt tgtccggcgt tcgcagctaa agcgcgttct aaacacatgg tacgtgaacg tgttgctgaa caggaaatct tcgcccagac tggctccgtt gaagagtctc ttttccacaa accggaattc aacgtcctcc tgaagaaccg ctatcaggaa actggcctga aagattttat ctctcgtctg ctggaacacc acggcgcact ggcgaccggt aaacacgacg cagcgcagaa ggctcaccag gaactcctga acttccataa ctctcagtac tttggtgaaa tccaggttgg caccccgcct gtatctttca tcgtagtttt cgacaccggc tcatccaacc tttggatccc tgcttccgaa tgcaagcagg gcggttgcgt tccgcacacc cgcttcgacc cgaaaacctc ttctacttac ctgccgataa acgcaggcgc tggcgaaccg gcaatcgcgt tcatccagta cggcactggt gcttgtgtac tgcgtatggc aaaagacacc gtgtccatcg gtggtattcg tgttcagaac cagaccttag gtctggcact ccaggaatcc gtacacccgt tcgcggacct gccgtttgac ggcctggttg gcctgggctt cccggacgtt gctggtgaag aaggtctgcc gccggatgct ctgccgctgg tcgacagcat gatgaaacag aaactgctga aacgtaacgc attcgctgta tacatgtctg aagatctgaa ccagccgggc gaaatcacct ttggctctgt taacccgcgc cacactttcg aaggccacaa accgcagtgg tttccggtca tctccctgga ttactgggag gtgggtgttc acggtcttcg cttgaaccgc aaatccctcg gtttttgcga acgtacccgt tgcaaagctg ctgttgacac tggctcttct ctgatcaccg gtccttctaa agtgatcaac ccgctgctga aatccctgaa cgtggctgaa gactgctcca acaaaggcaa cctgccgacc gttaccttca tcctggaaga caccgcgggc cgcctggtga aattcccgct gaagccgagc gactacctgg ttgaagaggt ggactcccaa ggtcgcctgg taacctgcgt tgctggtttt atccctatgg acgtcccggc tccgcgtggc ccgctgttcg tactgggtaa ctccttcatc cgtaagttct acaccatttt cgaccgtgac cacatgatgg ttggctttat gcgcgcgaac cacaacggtt ctggcccgtc tatgaaagaa agcagcggct cctctacttc catctactgg gcagcactgc tgggcgtaat gtctctgtgc gttcgcgcta ccgtataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for TogoA.17789.a.A12.GE33760
HARVESTED ON: 10/4/2011
SEQUENCED ON: 10/7/2011
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 73
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLFHTIAC LAVCCCPAFA AKARSKHMVR ERVAEQEIFA QTGSVEESLF HKPEFNVLLK NRYQETGLKD FISRLLEHHG ALATGKHDAA QKAHQELLNF HNSQYFGEIQ VGTPPVSFIV VFDTGSSNLW IPASECKQGG CVPHTRFDPK TSSTYLPINA GAGEPAIAFI QYGTGACVLR MAKDTVSIGG IRVQNQTLGL ALQESVHPFA DLPFDGLVGL GFPDVAGEEX LPPDALPLVD SMMKQKLLKR NAFAVYMSED LNQPGEITFG SVNPRHTFEG HKPQXXXGHL PGLLGVGVHG LRLXXKSLGF XXYPXXSCXX XXXX
Validated NT Sequence
attttgtttn nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgcgt ctgttccaca ccatcgcttg cctcgcagtg tgctgttgtc cggcgttcgc agctaaagcg cgttctaaac acatggtacg tgaacgtgtt gctgaacagg aaatcttcgc ccagactggc tccgttgaag agtctctttt ccacaaaccg gaattcaacg tcctcctgaa gaaccgctat caggaaactg gcctgaaaga ttttatctct cgtctgctgg aacaccacgg cgcactggcg accggtaaac acgacgcagc gcagaaggct caccaggaac tcctgaactt ccataactct cagtactttg gtgaaatcca ggttggcacc ccgcctgtat ctttcatcgt agttttcgac accggctcat ccaacctttg gatccctgct tccgaatgca agcagggcgg ttgcgttccg cacacccgct tcgacccgaa aacctcttct acttacctgc cgataaacgc aggcgctggc gaaccggcaa tcgcgttcat ccagtacggc actggtgctt gtgtactgcg tatggcaaaa gacaccgtgt ccatcggtgg tattcgtgtt cagaaccaga ccttaggtct ggcactccag gaatccgtac acccgttcgc ggacctgccg tttgacggcc tggttggcct gggcttcccg gacgttgctg gtgaagaang tctgccgccg gatgctctgc cgctggtcga cagcatgatg aaacagaaac tgctgaaacg taacgcattc gctgtataca tgtctgaaga tctgaaccag ccgggcgaaa tcacctttgg ctctgttaac ccgcgccaca ctttcgaagg ccacaaaccg cagnggnttn cnggtcatct ccctggatta ctgggngtgg gtgttcacgg tctncgcttg nanngcaaat ccctcggttt tncnangtac ccgttncnaa gctgctnnna nnctngnnnn nnntgatcnn nnnnnnctna nnatnnnnnc tncnnnaann cnnannnnnn nannctgnnc nnncnannnn nnnncnnnnn nnnncnnnnn nnnnnnnnnn nnnnnnnnnc nnannnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLFHTIAC LAVCCCPAFA AKARSKHMVR ERVAEQEIFA QTGSVEESLF HKPEFNVLLK NRYQETGLKD FISRLLEHHG ALATGKHDAA QKAHQELLNF HNSQYFGEIQ VGTPPVSFIV VFDTGSSNLW IPASECKQGG CVPHTRFDPK TSSTYLPINA GAGEPAIAFI QYGTGACVLR MAKDTVSIGG IRVQNQTLGL ALQESVHPFA DLPFDGLVGL GFPDVAGEEG LPPDALPLVD SMMKQKLLKR NAFAVYMSED LNQPGEITFG SVNPRHTFEG HKPQWFPVIS LDYWEVGVHG LRLNRKSLGF CERTRCKAAV DTGSSLITGP SKVINPLLKS LNVAEDCSNK GNLPTVTFIL EDTAGRLVKF PLKPSDYLVE EVDSQGRLVT CVAGFIPMDV PAPRGPLFVL GNSFIRKFYT IFDRDHMMVG FMRANHNGSG PSMKESSGSS T
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtctgttc cacaccatcg cttgcctcgc agtgtgctgt tgtccggcgt tcgcagctaa agcgcgttct aaacacatgg tacgtgaacg tgttgctgaa caggaaatct tcgcccagac tggctccgtt gaagagtctc ttttccacaa accggaattc aacgtcctcc tgaagaaccg ctatcaggaa actggcctga aagattttat ctctcgtctg ctggaacacc acggcgcact ggcgaccggt aaacacgacg cagcgcagaa ggctcaccag gaactcctga acttccataa ctctcagtac tttggtgaaa tccaggttgg caccccgcct gtatctttca tcgtagtttt cgacaccggc tcatccaacc tttggatccc tgcttccgaa tgcaagcagg gcggttgcgt tccgcacacc cgcttcgacc cgaaaacctc ttctacttac ctgccgataa acgcaggcgc tggcgaaccg gcaatcgcgt tcatccagta cggcactggt gcttgtgtac tgcgtatggc aaaagacacc gtgtccatcg gtggtattcg tgttcagaac cagaccttag gtctggcact ccaggaatcc gtacacccgt tcgcggacct gccgtttgac ggcctggttg gcctgggctt cccggacgtt gctggtgaag aaggtctgcc gccggatgct ctgccgctgg tcgacagcat gatgaaacag aaactgctga aacgtaacgc attcgctgta tacatgtctg aagatctgaa ccagccgggc gaaatcacct ttggctctgt taacccgcgc cacactttcg aaggccacaa accgcagtgg tttccggtca tctccctgga ttactgggag gtgggtgttc acggtcttcg cttgaaccgc aaatccctcg gtttttgcga acgtacccgt tgcaaagctg ctgttgacac tggctcttct ctgatcaccg gtccttctaa agtgatcaac ccgctgctga aatccctgaa cgtggctgaa gactgctcca acaaaggcaa cctgccgacc gttaccttca tcctggaaga caccgcgggc cgcctggtga aattcccgct gaagccgagc gactacctgg ttgaagaggt ggactcccaa ggtcgcctgg taacctgcgt tgctggtttt atccctatgg acgtcccggc tccgcgtggc ccgctgttcg tactgggtaa ctccttcatc cgtaagttct acaccatttt cgaccgtgac cacatgatgg ttggctttat gcgcgcgaac cacaacggtt ctggcccgtc tatgaaagaa agcagcggct cctctactta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for TogoA.17789.a.A13.GE33761
HARVESTED ON: 10/4/2011
SEQUENCED ON: 10/7/2011
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRERVAEQ EIFAQTGSVE ESLFHKPEFN VLLKNRYQET GLKDFISRLL EHHGALATGK HDAAQKAHQE LLNFHNSQYF GEIQVGTPPV SFIVVFDTGS SNLWIPASEC KQGGCVPHTR FDPKTSSTYL PINAGAGEPA IAFIQYGTGA CVLRMAKDTV SIGGIRVQNQ TLGLALQESV HPFADLPFDG LVGLGFPDVA GEEGLPPDAL PLVDSMMKQK LLKRNAFAVY MSEDLNQPGE ITFGSVNPRH TFEGHKPQWF PVISLDYWEV GVHGLRLNRK SLGFCERTRC KAAVDTGSSL ITGPSKVINP LLKSLNVAED CSNKGNLPTV TFILEDTAGR LVKFPLKPSD YLVEEVDSQG RLVTCVAGFI PMDVPAPRGP LFVLGNSFIR KFYTIFDRDH MMVGFMRANH NGSGPSMKES SGSSTSIYWA ALLGVMSLCV RATV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtacgtgaa cgtgttgctg aacaggaaat cttcgcccag actggctccg ttgaagagtc tcttttccac aaaccggaat tcaacgtcct cctgaagaac cgctatcagg aaactggcct gaaagatttt atctctcgtc tgctggaaca ccacggcgca ctggcgaccg gtaaacacga cgcagcgcag aaggctcacc aggaactcct gaacttccat aactctcagt actttggtga aatccaggtt ggcaccccgc ctgtatcttt catcgtagtt ttcgacaccg gctcatccaa cctttggatc cctgcttccg aatgcaagca gggcggttgc gttccgcaca cccgcttcga cccgaaaacc tcttctactt acctgccgat aaacgcaggc gctggcgaac cggcaatcgc gttcatccag tacggcactg gtgcttgtgt actgcgtatg gcaaaagaca ccgtgtccat cggtggtatt cgtgttcaga accagacctt aggtctggca ctccaggaat ccgtacaccc gttcgcggac ctgccgtttg acggcctggt tggcctgggc ttcccggacg ttgctggtga agaaggtctg ccgccggatg ctctgccgct ggtcgacagc atgatgaaac agaaactgct gaaacgtaac gcattcgctg tatacatgtc tgaagatctg aaccagccgg gcgaaatcac ctttggctct gttaacccgc gccacacttt cgaaggccac aaaccgcagt ggtttccggt catctccctg gattactggg aggtgggtgt tcacggtctt cgcttgaacc gcaaatccct cggtttttgc gaacgtaccc gttgcaaagc tgctgttgac actggctctt ctctgatcac cggtccttct aaagtgatca acccgctgct gaaatccctg aacgtggctg aagactgctc caacaaaggc aacctgccga ccgttacctt catcctggaa gacaccgcgg gccgcctggt gaaattcccg ctgaagccga gcgactacct ggttgaagag gtggactccc aaggtcgcct ggtaacctgc gttgctggtt ttatccctat ggacgtcccg gctccgcgtg gcccgctgtt cgtactgggt aactccttca tccgtaagtt ctacaccatt ttcgaccgtg accacatgat ggttggcttt atgcgcgcga accacaacgg ttctggcccg tctatgaaag aaagcagcgg ctcctctact tccatctact gggcagcact gctgggcgta atgtctctgt gcgttcgcgc taccgtataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for TogoA.17789.a.A14.GE33762
HARVESTED ON: 10/4/2011
SEQUENCED ON: 10/7/2011
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 86
PERCENT COVERAGE: 86
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVRERVAEQ EIFAQTGSVE ESLFHKPEFN VLLKNRYQET GLKDFISRLL EHHGALATGK HDAAQKAHQE LLNFHNSQYF GEIQVGTPPV SFIVVFDTGS SNLWIPASEC KQGGCVPHTR FDPKTSSTYL PINAGAGEPA IAFIQYGTGA CVLRMAKDTV SIGGIRVQNQ TLGLALQESV HPFADLPFDG LVGLGFPDVA GEEGLPPDAL PLVDSMMKQK LLXRNAFAVY MSEDLNQPGE ITFGSVNPRH TFEGHKPQWF PVISLDYWXV GVHGLRLNRK SLGFCERTRC KAAVDTGSSL ITGXSXVINP LLKSXXXXXL LXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXX
Validated NT Sequence
attttgtnnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtac gtgaacgtgt tgctgaacag gaaatcttcg cccagactgg ctccgttgaa gagtctcttt tccacaaacc ggaattcaac gtcctcctga agaaccgcta tcaggaaact ggcctgaaag attttatctc tcgtctgctg gaacaccacg gcgcactggc gaccggtaaa cacgacgcag cgcagaaggc tcaccaggaa ctcctgaact tccataactc tcagtacttt ggtgaaatcc aggttggcac cccgcctgta tctttcatcg tagttttcga caccggctca tccaaccttt ggatccctgc ttccgaatgc aagcagggcg gttgcgttcc gcacacccgc ttcgacccga aaacctcttc tacttacctg ccgataaacg caggcgctgg cgaaccggca atcgcgttca tccagtacgg cactggtgct tgtgtactgc gtatggcaaa agacaccgtg tccatcggtg gtattcgtgt tcagaaccag accttaggtc tggcactcca ggaatccgta cacccgttcg cggacctgcc gtttgacggc ctggttggcc tgggcttccc ggacgttgct ggtgaagaag gtctgccgcc ggatgctctg ccgctggtcg acagcatgat gaaacagaaa ctgctgnaac gtaacgcatt cgctgtatac atgtctgaag atctgaacca gccgggcgaa atcacctttg gctctgttaa cccgcgccac actttcgaag gccacaaacc gcagtggttt ccggtcatct ccctggatta ctgggangtg ggtgttcacg gtcttcgctt gaaccgcaaa tccctcggtt tttgcgaacg tacccgttgc aaagctgctg ttgacactgg ctcttctctg atcaccggtc nntctnaagt gatcaacccg ctnctgaaat cccngnangn nnnnannctg ctnnnaannn nnnncnacnt nnnnnnncnn nnncncngnn nnnnnnaann cnnnnancnn nnnntncnnn nnnnnnggnn tncnnncnnn nnnnnnnnng nnnnnnnnnn nnnnnnnnnn nnnnnngnnn nnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRERVAEQ EIFAQTGSVE ESLFHKPEFN VLLKNRYQET GLKDFISRLL EHHGALATGK HDAAQKAHQE LLNFHNSQYF GEIQVGTPPV SFIVVFDTGS SNLWIPASEC KQGGCVPHTR FDPKTSSTYL PINAGAGEPA IAFIQYGTGA CVLRMAKDTV SIGGIRVQNQ TLGLALQESV HPFADLPFDG LVGLGFPDVA GEEGLPPDAL PLVDSMMKQK LLKRNAFAVY MSEDLNQPGE ITFGSVNPRH TFEGHKPQWF PVISLDYWEV GVHGLRLNRK SLGFCERTRC KAAVDTGSSL ITGPSKVINP LLKSLNVAED CSNKGNLPTV TFILEDTAGR LVKFPLKPSD YLVEEVDSQG RLVTCVAGFI PMDVPAPRGP LFVLGNSFIR KFYTIFDRDH MMVGFMRANH NGSGPSMKES SGSST
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtacgtgaa cgtgttgctg aacaggaaat cttcgcccag actggctccg ttgaagagtc tcttttccac aaaccggaat tcaacgtcct cctgaagaac cgctatcagg aaactggcct gaaagatttt atctctcgtc tgctggaaca ccacggcgca ctggcgaccg gtaaacacga cgcagcgcag aaggctcacc aggaactcct gaacttccat aactctcagt actttggtga aatccaggtt ggcaccccgc ctgtatcttt catcgtagtt ttcgacaccg gctcatccaa cctttggatc cctgcttccg aatgcaagca gggcggttgc gttccgcaca cccgcttcga cccgaaaacc tcttctactt acctgccgat aaacgcaggc gctggcgaac cggcaatcgc gttcatccag tacggcactg gtgcttgtgt actgcgtatg gcaaaagaca ccgtgtccat cggtggtatt cgtgttcaga accagacctt aggtctggca ctccaggaat ccgtacaccc gttcgcggac ctgccgtttg acggcctggt tggcctgggc ttcccggacg ttgctggtga agaaggtctg ccgccggatg ctctgccgct ggtcgacagc atgatgaaac agaaactgct gaaacgtaac gcattcgctg tatacatgtc tgaagatctg aaccagccgg gcgaaatcac ctttggctct gttaacccgc gccacacttt cgaaggccac aaaccgcagt ggtttccggt catctccctg gattactggg aggtgggtgt tcacggtctt cgcttgaacc gcaaatccct cggtttttgc gaacgtaccc gttgcaaagc tgctgttgac actggctctt ctctgatcac cggtccttct aaagtgatca acccgctgct gaaatccctg aacgtggctg aagactgctc caacaaaggc aacctgccga ccgttacctt catcctggaa gacaccgcgg gccgcctggt gaaattcccg ctgaagccga gcgactacct ggttgaagag gtggactccc aaggtcgcct ggtaacctgc gttgctggtt ttatccctat ggacgtcccg gctccgcgtg gcccgctgtt cgtactgggt aactccttca tccgtaagtt ctacaccatt ttcgaccgtg accacatgat ggttggcttt atgcgcgcga accacaacgg ttctggcccg tctatgaaag aaagcagcgg ctcctctact taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for TogoA.17789.a.A15.GE33763
HARVESTED ON: 10/4/2011
SEQUENCED ON: 10/7/2011
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLFHKPEF NVLLKNRYQE TGLKDFISRL LEHHGALATG KHDAAQKAHQ ELLNFHNSQY FGEIQVGTPP VSFIVVFDTG SSNLWIPASE CKQGGCVPHT RFDPKTSSTY LPINAGAGEP AIAFIQYGTG ACVLRMAKDT VSIGGIRVQN QTLGLALQES VHPFADLPFD GLVGLGFPDV AGEEGLPPDA LPLVDSMMKQ KLLKRNAFAV YMSEDLNQPG EITFGSVNPR HTFEGHKPQW FPVISLDYWE VGVHGLRLNR KSLGFCERTR CKAAVDTGSS LITGPSKVIN PLLKSLNVAE DCSNKGNLPT VTFILEDTAG RLVKFPLKPS DYLVEEVDSQ GRLVTCVAGF IPMDVPAPRG PLFVLGNSFI RKFYTIFDRD HMMVGFMRAN HNGSGPSMKE SSGSSTSIYW AALLGVMSLC VRATV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctcttttc cacaaaccgg aattcaacgt cctcctgaag aaccgctatc aggaaactgg cctgaaagat tttatctctc gtctgctgga acaccacggc gcactggcga ccggtaaaca cgacgcagcg cagaaggctc accaggaact cctgaacttc cataactctc agtactttgg tgaaatccag gttggcaccc cgcctgtatc tttcatcgta gttttcgaca ccggctcatc caacctttgg atccctgctt ccgaatgcaa gcagggcggt tgcgttccgc acacccgctt cgacccgaaa acctcttcta cttacctgcc gataaacgca ggcgctggcg aaccggcaat cgcgttcatc cagtacggca ctggtgcttg tgtactgcgt atggcaaaag acaccgtgtc catcggtggt attcgtgttc agaaccagac cttaggtctg gcactccagg aatccgtaca cccgttcgcg gacctgccgt ttgacggcct ggttggcctg ggcttcccgg acgttgctgg tgaagaaggt ctgccgccgg atgctctgcc gctggtcgac agcatgatga aacagaaact gctgaaacgt aacgcattcg ctgtatacat gtctgaagat ctgaaccagc cgggcgaaat cacctttggc tctgttaacc cgcgccacac tttcgaaggc cacaaaccgc agtggtttcc ggtcatctcc ctggattact gggaggtggg tgttcacggt cttcgcttga accgcaaatc cctcggtttt tgcgaacgta cccgttgcaa agctgctgtt gacactggct cttctctgat caccggtcct tctaaagtga tcaacccgct gctgaaatcc ctgaacgtgg ctgaagactg ctccaacaaa ggcaacctgc cgaccgttac cttcatcctg gaagacaccg cgggccgcct ggtgaaattc ccgctgaagc cgagcgacta cctggttgaa gaggtggact cccaaggtcg cctggtaacc tgcgttgctg gttttatccc tatggacgtc ccggctccgc gtggcccgct gttcgtactg ggtaactcct tcatccgtaa gttctacacc attttcgacc gtgaccacat gatggttggc tttatgcgcg cgaaccacaa cggttctggc ccgtctatga aagaaagcag cggctcctct acttccatct actgggcagc actgctgggc gtaatgtctc tgtgcgttcg cgctaccgta taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for TogoA.17789.a.A16.GE33764
HARVESTED ON: 10/4/2011
SEQUENCED ON: 10/7/2011
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 88
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSLFHKPEF NVLLKNRYQE TGLKDFISRL LEHHGALATG KHDAAQKAHQ ELLNFHNSQY FGEIQVGTPP VSFIVVFDTG SSNLWIPASE CKQGGCVPHT RFDPKTSSTY LPINAGAGEP AIAFIQYGTG ACVLRMAKDT VSIGGIRVQN QTLGLALQES VHPFADLPFD GLVGLGFPDV AGEEGLPPDA LPLVDSMMKQ KLLKRNAFAV YMSEDLNQPG EITFGSVNPR HTFEGHKPQW FPVISLDYWE VGVHGLRLNR KSLGFCERTR CKAAVDTGSS LITGPSKVIX PLLKSLNXXX LLXKXXXXXX LXXXXXXXXX XXXTXXXXXX XXXXXXXXXP XXXXXXXXXX XXXXX
Validated NT Sequence
ttttntttnn ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtctc ttttccacaa accggaattc aacgtcctcc tgaagaaccg ctatcaggaa actggcctga aagattttat ctctcgtctg ctggaacacc acggcgcact ggcgaccggt aaacacgacg cagcgcagaa ggctcaccag gaactcctga acttccataa ctctcagtac tttggtgaaa tccaggttgg caccccgcct gtatctttca tcgtagtttt cgacaccggc tcatccaacc tttggatccc tgcttccgaa tgcaagcagg gcggttgcgt tccgcacacc cgcttcgacc cgaaaacctc ttctacttac ctgccgataa acgcaggcgc tggcgaaccg gcaatcgcgt tcatccagta cggcactggt gcttgtgtac tgcgtatggc aaaagacacc gtgtccatcg gtggtattcg tgttcagaac cagaccttag gtctggcact ccaggaatcc gtacacccgt tcgcggacct gccgtttgac ggcctggttg gcctgggctt cccggacgtt gctggtgaag aaggtctgcc gccggatgct ctgccgctgg tcgacagcat gatgaaacag aaactgctga aacgtaacgc attcgctgta tacatgtctg aagatctgaa ccagccgggc gaaatcacct ttggctctgt taacccgcgc cacactttcg aaggccacaa accgcagtgg tttccggtca tctccctgga ttactgggag gtgggtgttc acggtcttcg cttgaaccgc aaatccctcg gtttttgcga acgtacccgt tgcaaagctg ctgttgacac tggctcttct ctgatcaccg gtccntctaa agtgatcnan ccgctgctga aatccctgaa cgngnngnnn ctgctcanna agnnnnnnnn ncnnnnnnnn ctnnnanncn cggnnncnnn nnanncnnct nancnnnnna ctncnnnnna nangngnntn nnnncnnnnn nnnngnnnnc nnnnnnnccn nnnnngnnnn ntnnnnnnnn nnnnnnnanc nnnnnnnnnn nnnntt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLFHKPEF NVLLKNRYQE TGLKDFISRL LEHHGALATG KHDAAQKAHQ ELLNFHNSQY FGEIQVGTPP VSFIVVFDTG SSNLWIPASE CKQGGCVPHT RFDPKTSSTY LPINAGAGEP AIAFIQYGTG ACVLRMAKDT VSIGGIRVQN QTLGLALQES VHPFADLPFD GLVGLGFPDV AGEEGLPPDA LPLVDSMMKQ KLLKRNAFAV YMSEDLNQPG EITFGSVNPR HTFEGHKPQW FPVISLDYWE VGVHGLRLNR KSLGFCERTR CKAAVDTGSS LITGPSKVIN PLLKSLNVAE DCSNKGNLPT VTFILEDTAG RLVKFPLKPS DYLVEEVDSQ GRLVTCVAGF IPMDVPAPRG PLFVLGNSFI RKFYTIFDRD HMMVGFMRAN HNGSGPSMKE SSGSST
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctcttttc cacaaaccgg aattcaacgt cctcctgaag aaccgctatc aggaaactgg cctgaaagat tttatctctc gtctgctgga acaccacggc gcactggcga ccggtaaaca cgacgcagcg cagaaggctc accaggaact cctgaacttc cataactctc agtactttgg tgaaatccag gttggcaccc cgcctgtatc tttcatcgta gttttcgaca ccggctcatc caacctttgg atccctgctt ccgaatgcaa gcagggcggt tgcgttccgc acacccgctt cgacccgaaa acctcttcta cttacctgcc gataaacgca ggcgctggcg aaccggcaat cgcgttcatc cagtacggca ctggtgcttg tgtactgcgt atggcaaaag acaccgtgtc catcggtggt attcgtgttc agaaccagac cttaggtctg gcactccagg aatccgtaca cccgttcgcg gacctgccgt ttgacggcct ggttggcctg ggcttcccgg acgttgctgg tgaagaaggt ctgccgccgg atgctctgcc gctggtcgac agcatgatga aacagaaact gctgaaacgt aacgcattcg ctgtatacat gtctgaagat ctgaaccagc cgggcgaaat cacctttggc tctgttaacc cgcgccacac tttcgaaggc cacaaaccgc agtggtttcc ggtcatctcc ctggattact gggaggtggg tgttcacggt cttcgcttga accgcaaatc cctcggtttt tgcgaacgta cccgttgcaa agctgctgtt gacactggct cttctctgat caccggtcct tctaaagtga tcaacccgct gctgaaatcc ctgaacgtgg ctgaagactg ctccaacaaa ggcaacctgc cgaccgttac cttcatcctg gaagacaccg cgggccgcct ggtgaaattc ccgctgaagc cgagcgacta cctggttgaa gaggtggact cccaaggtcg cctggtaacc tgcgttgctg gttttatccc tatggacgtc ccggctccgc gtggcccgct gttcgtactg ggtaactcct tcatccgtaa gttctacacc attttcgacc gtgaccacat gatggttggc tttatgcgcg cgaaccacaa cggttctggc ccgtctatga aagaaagcag cggctcctct acttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa