TogoA.17255.a

MutS-like protein

CENTER ID: TogoA.17255.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.17255.a.A5.GE30008 DNA repair protein MutS C-term domain 1324 1801
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_218840
UniProt: B6DST1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGGDSVCSDR DQEWRFSSRQ EFFCSIASRA LRPASGPGAP LRGSASFARY RWKLQTILFS LVCISVEVNA RLLMEMFPRQ RGCRQGLACS VPQAYWSINA LLSANKMHGR TSKSFLQKVA KRDSSCGGWA TLGQHQLLDF FSAAARDAAG QLRGCRASLP FLASSRRVAF VVHPDRRLAR PTIQLPHAIS DRQTHFPSNA SRLSGRFFHT QKRPEAPTRD NLLGPKPSRR AEENCSVLRS SAESCPPPSV AVHPTFLARL PLLSRPASRR LLSFLSFDHP SMVPDSLHMP PLLVFVREQK RLRPDKVLII QVGDFFEAYG LDAVLLVEYC GLNPMGGKAK AGCPKQNLQQ TLDCLTSEGF SVSVFEEFAS PVAATPIAPG NRKLRLLTQI VSPSSPLYLP AHMPLYEGEI GAHTPDSEPI FSLYYSPSAG YACGEIDVPQ RTLRVEGGLT ADGVEMRITS ATGGAPRGEL YVHQMSALGV RDDGTTRLAK RLFPSLQKIY LRDYSSPSAF HRAVLRHLTA SLQVPHSEFT IRSHLDYFSP SVAVPEPAAS PADPCLLSPS ELGASQAAPP RNGSLSFPEN RAPFPPEPQL STAALSAEDA RRQAETLERG LAEDGRARTD AALRSASEES PAEPPRQNSG EVVSPHSQQV PPLLARPQKH RPHPLYVSTA SQLGLLRPSG RQGNTVASTS SFVVPPLHLA ALPPGAPVTS TRFIQRLLLF PPPHAVADAV QNILRQLLRL GCTDSRLSST FMKHFPGSSS PSAERSQAPT GKEEGDGVCV GCQSPPRATD FSAKNRSSER GLAQSGQAAA LDSSAENLAT HAENSDVLLV PSVRVMDAGK LVQVISSRSA NRRFFLEVLT LLAPTRYCLR RYPPSLLKPL LTVLQHETQQ QVCQERFLQG VEKAIALIEQ VLLLEEEEQE AAAEGAALAA IAAAEQMSRL AASEVQGRPF ENLSPHSQSA QSGENSTFGG DSRDIYSAAD AAAAAARAVA EAAAAREAAE EAAARAMATV AGGHATSSST RGLLPILDDY FKRMERFRGH IQPFCVPYAH ALVAAAVDDL LVSIFEDFAG AARMPALPAV CTLEEFLPSR SRAPGGEKTP LTARDVVDLF IALERRQTPG KGSAAPSARL AWLLEKQTPV RLKIPPAALF SSIAALQRQR LAAAKSGDFG RPAGEWLRLI AALQKKLWGD TLNESVYLKK GKPPLEPVKA ISRNGREKNA KSSAALTPEF ECGVAEAQAA NAVTHGVERS SCREAFEALG ETPGEALVSR EEGMGSGLRV TERATDLHAG EFGTNEREAN EQRSAVATTP HWSKTPEHPR DRHGHQIASA WTTPRVAACS RAYTAAKNFA ASAVEKRLRE LSRDLQPLLP DILLAAHLII ILQSLSHHST FAATNGWHLP QLLDPNAEAE LKVDTLTPYY MRRPDLNTLR RQRAVQRDRA ALLDREDRGS DTNRIAESRR TGTKNEELPT DDEFANANRT QSRDPLSEQV QKDTASLLAH ASSSLSKERE VVSYSFDMHG LFVLTGENMA GKSTFCRSVL ALVVLANAGL FCPCGPATTV PRYGAFLASS YMAHDSPLEN KSFFYEELSQ LHNILSQARS YGKVLPEKTA ALPASLLSSD TSDAGVSLRT ETADVALTTP FVIVDEPCKG TAPKWGAGFV GAALELLCRG NTHGVLSTHL HHELSRLPLD LQPNVRFKRM GVRKRNPTSL VLPESCSGPA SLTGGRDLPR FKSSQPLSSP LDSDKRTHFA AGSLGDDRLY TFELLDGICQ DSNAMQTAWK VGMPSGIITR ANALQAHLDA VDQGKIPCKI GGVSRGAVIK DGDGANTGIS SETSDEIVSS GTIAEKRFPE MHETPKQPPD ELPRHRNENG QEPQGTTADV SETVQLHTFH QPNPENAGSP NGEPTPCGGI GLFAEPQGIV DFPNRSASVM SAFASSAMAS NALLARLRLA MIETTRELTC ANAPAECVRN QAESRNNALH SVDTNAERPP LKIASAKDVL PVLELPALSR RLAAPPPWQD CSACVYMLVA PCTAARNAHR RAYLASEVPM RRPPDFPAQE ATRSRASADE PAGFCANVYV GETERLETRL RTHSRSPLWA HARVLVVRVK NKAEAREVET ALIRRLKEEK GVVLMSLKDG NRRASGLSVR EDVSTSESQD GRD
NT Sequence
atgggagggg attctgtatg cagtgaccgt gaccaggaat ggcgattctc ctctaggcag gaattttttt gctccattgc ctctcgcgct ctgcggcccg cttcgggtcc gggggcgcct ctccgcggct ccgcttcttt cgcgcgctat cgctggaaac tgcagacgat tctcttttca cttgtgtgca tctcagtgga ggtcaacgcg agactgctga tggaaatgtt tccgcgacag cgcggttgcc ggcagggttt agcctgctcc gttccgcaag cgtactggtc gatcaatgcc ttgctttccg cgaacaagat gcacggccgc acgtccaaga gttttttaca gaaggtcgcg aaacgcgaca gcagctgcgg agggtgggcc actcttgggc aacatcagct gctggacttc ttttcggcag ctgcaaggga cgcagcaggc cagttgcgtg gctgtcgcgc cagcttgccc tttcttgcct cttctcgacg cgtcgctttt gttgtccatc cggaccgacg cctcgcacgt ccgaccattc agttgcctca cgcgatttca gacaggcaga ctcatttccc ttcaaacgca tcccgcctgt ccggccgctt cttccacacg cagaagcgtc cggaagcgcc gacgcgcgac aacctgctgg gaccgaagcc gagtcgaaga gcggaagaaa actgctccgt tctgcgttcc tctgcagaaa gctgcccgcc tccctcggtg gcagtacacc cgacgttctt ggcaaggctg ccgcttcttt cccgtcccgc ttcccggcgg ctgctctctt ttctttcctt cgaccatccg tcgatggttc ctgactcgct gcatatgccg cctctcctcg tcttcgtacg agaacaaaaa cgacttcggc ccgacaaagt tctgattatt caggttggag acttcttcga ggcatacggc ctcgatgctg ttttgctggt ggagtactgc gggctgaatc cgatgggtgg gaaggcaaag gcggggtgcc ccaaacaaaa cctgcagcaa acgctcgatt gtttaacgag tgaaggcttc tccgtgtccg tttttgagga gttcgcgtct cctgtggcgg cgactccaat cgcacctgga aatcgaaagc ttcgcctcct tacgcaaatt gtctctcctt cttcacccct gtatctccca gcccacatgc ctctttatga gggcgagatc ggagcgcaca ctccagactc agagccgatt ttttcgctgt actactcccc gagtgcaggt tacgcttgcg gagaaatcga tgttccacag agaactttga gagtggaggg cggcttgact gccgacggcg ttgaaatgcg aattacgtcg gccacaggcg gcgcacctcg aggcgagctg tatgtacacc agatgtcggc gctcggcgtg cgagacgacg ggaccactcg gctggccaag cgactctttc cctctctcca gaaaatatac ttgcgggact acagttcgcc gtcggccttc caccgcgccg tgctgcggca cctgaccgcc tctctgcaag tgcctcattc tgagttcacc attcgctcgc acttggacta tttctctccc tctgtggcgg ttccggaacc cgctgcgagt cccgcagatc cctgcctgct gtccccatcc gagctggggg cctctcaagc tgcccctccc cgaaacggat ccttgtcttt tccagagaac cgcgcgccct ttccaccaga gcctcaactg tcaactgccg ccctgtccgc agaggatgct cgaaggcagg ctgaaactct agagcgcgga ctcgctgaag acggacgcgc gagaactgac gcagctctcc gctctgcatc agaggagtcg cctgcagaac cgccacgaca gaacagcgga gaagtcgttt cgccgcacag ccagcaagtt ccgccactcc tcgcgaggcc acagaagcac cgaccgcatc cgttgtatgt gtccacggca tcgcagctgg gccttctcag accttcaggc agacaaggaa acaccgtcgc ttccacttcg tccttcgtcg tccctcctct tcacctcgcc gccctccctc ctggcgctcc cgtcacttcc acgcgattca ttcaaaggct gctcctcttc ccgcctccgc atgcggtcgc cgacgcagtg cagaacattc ttcggcagct gctgcgtctc gggtgtacag acagccgact gtcttccacc ttcatgaagc actttccggg aagttcatcg ccttctgctg aacgctcaca ggctccgaca gggaaggaag aaggcgacgg cgtttgtgtc ggttgtcaat cgcccccaag agcgaccgac ttctctgcaa agaatcggtc ttccgaaaga ggactggcac agagcggaca agcggccgcc ctagacagca gcgcagagaa tttggcgacg catgcagaga acagcgatgt gcttctcgta ccctcagtcc gagtcatgga cgccgggaag ctggtgcaag taatttcgtc acgctcggcc aatcgaagat tctttctaga agttctgact ctcctcgcac ctacgcgcta ctgcctccgt cggtaccctc cttctctgct caagcccctc ctcaccgttc tccaacatga gacgcagcag caggtctgtc aggagcgatt cctgcaaggc gtcgagaagg ccattgctct gattgaacag gtccttctcc tggaagaaga ggaacaagaa gcagcagcgg agggagcagc gctggcggcg atcgctgcag ctgaacagat gagtcgcttg gcggcatctg aggtgcaggg aaggcccttt gagaatctct ctccccactc gcagtctgcg cagtcgggag agaactcaac gttcggagga gattctcgag acatctacag cgccgcagat gcagcagcag cggctgcgcg tgcagtggcg gaagctgcag cagctcgcga ggcggctgag gaggccgctg ctcgggcgat ggcgaccgtc gcagggggtc acgcgacgag cagcagcact cgaggtttgt tgccaattct cgacgattat ttcaagcgga tggagcgttt tcgtgggcat atccagccat tctgcgttcc ctacgcgcat gcacttgtgg cggctgcggt ggacgacctt ttggtgtcga ttttcgagga ctttgcgggc gcagccagga tgccggctct cccggctgtc tgtacactcg aggagtttct cccttctcgc tctcgcgcgc ctggggggga gaagacgccg ctgacagcac gagacgtcgt cgaccttttc atcgctctgg agcgcaggca aaccccaggg aagggatccg cggcgccctc agctcgtctc gcctggctgc tggaaaagca gacaccggtg cgcctgaaga ttcctcccgc agctctcttt tcgagcatcg cggctctcca gcgccagcgg ctggctgcgg caaagtcggg cgacttcggg agaccagctg gcgagtggct tcgcctcatt gcagcgctcc agaaaaaact ctggggcgac acgctgaatg agtcggtgta tttgaagaag ggtaaacccc cgttggaacc ggtgaaggcc atcagtcgaa acgggagaga aaagaacgcg aaaagttctg ctgctctgac ccccgaattt gagtgtggag tcgcggaggc gcaggcggcg aacgccgtga cacacggagt cgagaggagt tcttgccgcg aggccttcga ggcactgggc gagactccag gggaagcgct tgtgtccaga gaggagggga tgggcagcgg gctgcgtgtg accgagagag ccaccgactt gcatgcagga gaattcggga caaacgaaag agaggccaac gagcaaagaa gtgctgtggc taccactcca cactggagca agacgcctga acaccctagg gacagacacg gtcaccagat tgcctccgca tggacaacgc ctcgagtagc agcctgttca cgagcgtaca ctgcggcgaa gaatttcgca gcttctgcag ttgagaagag actgagggaa ctcagcagag atcttcagcc gttgctgccc gacattttgc tggcggccca cctcatcatc attctccaga gtctgagcca ccattcgact ttcgcggcaa ctaatgggtg gcatttaccc cagctcctgg acccaaatgc tgaagcagaa ctcaaggtcg atactttgac tccatactac atgcgtagac ccgatctgaa cacccttcgc cgccagcgtg cggtccagag agaccgtgcc gcgttactgg acagagaaga tcgcggaagc gacacaaacc gaatagcgga atcgagaagg accgggacaa aaaacgaaga acttccaact gacgatgagt ttgccaacgc aaacaggaca cagagtcggg accccctctc agagcaggtg cagaaggaca cggcttcgct cctggcgcat gcatcaagct cgctgtcgaa ggagcgggag gttgtgagct actccttcga tatgcacggg ttgtttgttt taacggggga gaacatggcc ggtaaaagca ccttctgtcg gtctgttctc gcactcgtcg tcctcgcaaa cgcaggttta ttttgcccct gtgggccagc cacgacggta cctcggtacg gggcgttcct cgcgtcgtcg tacatggctc acgactcgcc cttggagaac aaatcctttt tctatgaaga attgtctcag ctccacaaca tcctctctca ggcacggagc tacggtaaag tcctacctga gaaaacagcg gctcttcccg cctccttgct ctcaagcgac acctcagatg ccggtgtttc tctccggacg gaaactgcag acgttgcatt aactaccccg tttgtcattg ttgatgaacc ttgcaaaggc acagcgccta aatggggagc agggtttgtt ggtgcggcct tggagctgct ttgccgcggg aacacccacg gcgtattgtc aacccatctt catcacgaac tgagccgcct ccctctcgac cttcagccga acgtccggtt caagcgcatg ggcgtccgca agagaaaccc cacctctctc gtgcttcctg aaagctgctc cggaccagca tcactcaccg gaggacggga tttaccgcgg ttcaaaagtt cacagccctt gagttccccc ctcgactctg acaaaagaac gcacttcgcg gcagggtctc tcggtgatga taggttatac acgttcgaac tcctagacgg catttgccaa gatagcaacg ccatgcagac tgcgtggaaa gtgggcatgc cttcaggaat catcacgaga gcaaatgcac tgcaagcaca cttagatgcg gtagatcagg ggaaaatccc ttgtaagatc ggcggcgttt ctcggggggc tgttatcaaa gacggggatg gcgctaacac gggaatctct tccgaaacga gcgatgaaat tgtaagcagc ggaacgattg cggaaaaacg atttccagaa atgcacgaaa ccccaaaaca gcctccagac gaactaccgc gtcacagaaa cgaaaacggc caggaacctc aaggtacgac agcggatgtc tcggagactg tacagctaca cacgtttcac caacccaatc ccgagaatgc aggttcgcca aacggagaac cgacaccgtg tggcggtatc ggtttgttcg ccgagccaca aggaatcgta gattttccga atcgaagcgc ctctgtgatg agtgcttttg cgtcttccgc aatggcctct aacgctctcc ttgcacgcct gcggttggcg atgatcgaaa ccacccgaga actgacttgc gcgaacgcgc ctgccgaatg tgttcggaac caagcagagt cacgaaacaa cgcacttcac tcggtcgata cgaatgccga aagacctccg ttaaagatag cttccgcaaa agatgttctt ccagtgctcg agctaccggc cctgtcgaga cgcttagcag ctccgccgcc ttggcaagat tgctctgctt gtgtttatat gcttgtggct ccgtgtactg cggcacgaaa tgcacatcgg cgtgcatatc tcgcgagtga agtgcctatg cgccggccgc cggacttccc agctcaggaa gcgacgcgct ctcgagcaag tgcggacgaa ccggcagggt tctgtgccaa tgtttatgtg ggtgaaacag aacgccttga gacgcgtttg cgaactcaca gccgttcgcc gctgtgggct catgcccgcg tgttggttgt gagagtgaaa aacaaggcag aagcgcgaga ggttgagacc gccctgatta gaagactcaa ggaagaaaag ggcgtcgtcc tcatgtcact aaaggacggg aaccgtcgcg catcgggact gagtgtcagg gaggatgttt caacttccga gtcacaggat gggagagact ga
Details for TogoA.17255.a.A5.GE30008
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 54kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 76
PERCENT COVERAGE: 80
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGHQIASAW TTPRVAACSR AYTAAKNFAA SAVEKRLREL SRDLQPLLPD ILLAAHLIII LQSLSHHSTF AATNGWHLPQ LLDPNAEAEL KVDTLTPYYM RRPDLNTLRR QRAVQRDRAA LLDREDRGSD TNRIAESRRT GTKNEELPTD DEFANANRTQ SRDPLSEQVQ KDTASLLAHA SSSLSKEREV VSYSFDMHGL FVLTGENMXG KSTFCRSVLA LVVLANAGLF CPCGPATTVP RXGAFLASSY MXHDSPLXXK SFFYEELSQL HNILSQXRXY GKVXPXXTXA LPASLXXXXL XCXCXXXXGX XXVALXPXXX XXXXXXXAXX LXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
ttttgtnnnn nnttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggtc accagattgc ctccgcatgg acaacgcctc gagtagcagc ctgttcacga gcgtacactg cggcgaagaa tttcgcagct tctgcagtcg agaagagact gagggaactc agcagagatc ttcagccgtt gctgcccgac attttgctgg cggcccacct catcatcatt ctccagagtc tgagccacca ttcgactttc gcggcaacta atgggtggca tttaccccag ctcctggacc caaatgctga agcagaactc aaggtcgata ctttgactcc atactacatg cgtagacccg atctgaacac ccttcgccgc cagcgtgcgg tccagagaga ccgtgccgcg ttactggaca gagaagatcg cggaagcgac acaaaccgaa tagcggaatc gagaaggacc gggacaaaaa acgaagaact tccaactgac gatgagtttg ccaacgcaaa caggacacag agtcgggacc ccctctcaga gcaggtgcag aaggacacgg cttcgctcct ggcgcatgca tcaagctcgc tgtcgaagga gcgggaggtt gtgagctact ccttcgatat gcacgggttg tttgttttaa cgggggagaa catggncggt aaaagcacct tctgtcggtc tgttctcgca ctcgtcgtcc tcgcaaacgc aggtttattt tgcccctgtg ggccagccac gacggtacct cggnacgggg cgttcctcgc gtcgtcgtac atggntcacg actcgccctt gnagaanaaa tcctttttct atgaagaatt gtctcagctc cacaacatcc tctctcaggn acggngctac ggtaaagtnn tacctganna aacancngct cttcccgcct ccttgnnnna nnnananctc nnntgcnngt gtttntnnnc gganggnnnn gnnnacgttg cattantacc ccnnntgncn tnnntgatna ncnnngnnaa ngcacannnn ctaannngnn nncnngnttn gntgnnnngn nnngnnnnnn nnntttnncn nnngnnnnnc cnnnnngnnn nnnnnancnn nnntncnnnn nnagnnnnnn cntnnnnnnn nnnnnnnnnn nnncnnnnnn nnnnanggnn nnnnnnaann nnnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGHQIASAW TTPRVAACSR AYTAAKNFAA SAVEKRLREL SRDLQPLLPD ILLAAHLIII LQSLSHHSTF AATNGWHLPQ LLDPNAEAEL KVDTLTPYYM RRPDLNTLRR QRAVQRDRAA LLDREDRGSD TNRIAESRRT GTKNEELPTD DEFANANRTQ SRDPLSEQVQ KDTASLLAHA SSSLSKEREV VSYSFDMHGL FVLTGENMAG KSTFCRSVLA LVVLANAGLF CPCGPATTVP RYGAFLASSY MAHDSPLENK SFFYEELSQL HNILSQARSY GKVLPEKTAA LPASLLSSDT SDAGVSLRTE TADVALTTPF VIVDEPCKGT APKWGAGFVG AALELLCRGN THGVLSTHLH HELSRLPLDL QPNVRFKRMG VRKRNPTSLV LPESCSGPAS LTGGRDLPRF KSSQPLSSPL DSDKRTHFAA GSLGDDRLYT FELLDGICQD SNAMQTAWKV GMPSGIITRA NALQAHLDAV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtcaccag attgcctccg catggacaac gcctcgagta gcagcctgtt cacgagcgta cactgcggcg aagaatttcg cagcttctgc agttgagaag agactgaggg aactcagcag agatcttcag ccgttgctgc ccgacatttt gctggcggcc cacctcatca tcattctcca gagtctgagc caccattcga ctttcgcggc aactaatggg tggcatttac cccagctcct ggacccaaat gctgaagcag aactcaaggt cgatactttg actccatact acatgcgtag acccgatctg aacacccttc gccgccagcg tgcggtccag agagaccgtg ccgcgttact ggacagagaa gatcgcggaa gcgacacaaa ccgaatagcg gaatcgagaa ggaccgggac aaaaaacgaa gaacttccaa ctgacgatga gtttgccaac gcaaacagga cacagagtcg ggaccccctc tcagagcagg tgcagaagga cacggcttcg ctcctggcgc atgcatcaag ctcgctgtcg aaggagcggg aggttgtgag ctactccttc gatatgcacg ggttgtttgt tttaacgggg gagaacatgg ccggtaaaag caccttctgt cggtctgttc tcgcactcgt cgtcctcgca aacgcaggtt tattttgccc ctgtgggcca gccacgacgg tacctcggta cggggcgttc ctcgcgtcgt cgtacatggc tcacgactcg cccttggaga acaaatcctt tttctatgaa gaattgtctc agctccacaa catcctctct caggcacgga gctacggtaa agtcctacct gagaaaacag cggctcttcc cgcctccttg ctctcaagcg acacctcaga tgccggtgtt tctctccgga cggaaactgc agacgttgca ttaactaccc cgtttgtcat tgttgatgaa ccttgcaaag gcacagcgcc taaatgggga gcagggtttg ttggtgcggc cttggagctg ctttgccgcg ggaacaccca cggcgtattg tcaacccatc ttcatcacga actgagccgc ctccctctcg accttcagcc gaacgtccgg ttcaagcgca tgggcgtccg caagagaaac cccacctctc tcgtgcttcc tgaaagctgc tccggaccag catcactcac cggaggacgg gatttaccgc ggttcaaaag ttcacagccc ttgagttccc ccctcgactc tgacaaaaga acgcacttcg cggcagggtc tctcggtgat gataggttat acacgttcga actcctagac ggcatttgcc aagatagcaa cgccatgcag actgcgtgga aagtgggcat gccttcagga atcatcacga gagcaaatgc actgcaagca cacttagatg cggtaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa