TogoA.17220.a

unnamed apical complex protein

CENTER ID: TogoA.17220.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.17220.a.A1.GE29388 full length 1 394
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_316250
OrthoMCL: OG5_241114
RefSeq: XP_002364792.1
UniProt: B6K9R8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKGVSSRCSA LLRGGCLALA VLAALERLFT VADNVATAEN VQSGFPPLGV SPLEGTTQVG SSPRRLMSEG LSMMVMQYLL FPEMRESLVD LGAKSMEILK GGPTYDVLLD YYYDPQNCVV AFDTLYDGTF FGLVNLDSVK VTYSHVQHQG VLDYVESRRD TVEVLKRKQR HVQKFNLPKG CVADEDSYWV GYCRSVSPPC YLGRIDVHLS KGVPNEQAVP LEPIPYEEFV EQLVEREGMG FYDVEDMFDA ETPMYRLTWD PEKCFAHFRY FTDDLAATAY YWIQSRQAFR IDVKNPEDKF VRKMSAMGAD CGGEGPHEFS VTYKRLEDGS YKALFRARKL NDYEPRHTDT IELKEAKPNF PVQDWDPEMG EVMDFIDLFP EVDAVALDMM AKEQ
NT Sequence
atgaagggag tctcatctcg gtgctccgcc cttctgcggg gtggctgcct cgcgttggcg gtgctggcgg cactcgaacg gttgttcact gttgctgaca acgttgcaac tgcggaaaat gtgcaaagcg gctttccacc gctaggcgtc tcgccgctcg aaggcacaac tcaggtgggg tcgtcgccgc ggcggctgat gagtgaggga ctatcaatga tggtgatgca gtatttgctg tttccggaaa tgcgagagtc tctggttgat ctcggggcga agagcatgga aattctcaaa ggcgggccca cgtacgacgt gcttttggat tactactacg acccgcaaaa ctgcgtcgtc gcctttgaca cgctgtacga cgggactttt ttcggtttgg tgaatttaga ctcggtgaaa gtcacgtaca gccacgtcca gcaccagggc gtgctcgact acgtcgagag cagacgcgac actgtggaag tgctgaagcg gaagcagcgg cacgtgcaaa agttcaacct ccccaaaggg tgcgttgcag acgaggattc ctactgggta ggatattgcc gcagcgtctc cccgccctgc tacctcgggc ggatcgacgt gcacctttcg aagggtgttc ctaatgaaca ggctgtgcct ctggaaccca ttccgtacga agaatttgtt gaacagttgg tggaaagaga gggtatgggc ttttacgacg tcgaggacat gttcgatgcg gaaaccccca tgtaccgtct cacgtgggat ccagaaaagt gctttgccca cttccgatac ttcaccgacg acctggcggc gaccgcgtac tactggattc agagtcgcca ggcctttcga atcgacgtga agaatccgga ggacaaattc gtgagaaaaa tgtccgcaat gggggcagac tgcggcggag agggccccca cgagttctca gtgacctaca agcggctcga agacggctcg tacaaggcgc tcttccgcgc gcgcaagctc aacgactacg aaccccgtca cacggacacg attgaactga aagaagcgaa accaaacttc ccagttcagg actgggaccc cgagatgggc gaagtcatgg acttcattga cttattccca gaagtcgatg ctgttgctct cgacatgatg gctaaggaac agtaa
Details for TogoA.17220.a.A1.GE29388
HARVESTED ON: 5/3/2010
SEQUENCED ON: 5/6/2010
EXPECTED MW: 47kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MKGVSSRCSA LLRGGCLALA VLAALERLFT VADNVATAEN VQSGFPPLGV SPLEGTTQVG SSPRRLMSEG LSMMVMQYLL FPEMRESLVD LGAKSMEILK GGPTYDVLLD YYYDPQNCVV AFDTLYDGTF FGLVNLDSVK VTYSHVQHQG VLDYVESRRD TVEVLKRKQR HVQKFNLPKG CVADEDSYWV GYCRSVSPPC YLGRIDVHLS KGVPNEQAVP LEPIPYEEFV EQLVEREGMG FYDVEDMFDA ETPMYRLTWD PEKCFAHFRY FTDDLAATAY YWIQSRQAFR IDVKNPEDKF VRKMSAMGAD CGGEGPHEFS VTYKRLEDGS YKALFRARKL NDYEPRHTDT IELKEAKPNF PVQDWDPEMG EVMDFIDLFP EVDAVALDMM AK
Validated NT Sequence
accctggaag ctcagaccca gggtcctggt tcgatgaagg gagtctcatc tcggtgctcc gcccttctgc ggggtggctg cctcgcgttg gcggtgctgg cggcactcga acggttgttc actgttgctg acaacgttgc aactgcggaa aatgtgcaaa gcggctttcc accgctaggc gtctcgccgc tcgaaggcac aactcaggtg gggtcgtcgc cgcggcggct gatgagtgag ggactatcaa tgatggtgat gcagtatttg ctgtttccgg aaatgcgaga gtctctggtt gatctcgggg cgaagagcat ggaaattctc aaaggcgggc ccacgtacga cgtgcttttg gattactact acgacccgca aaactgcgtc gtcgcctttg acacgctgta cgacgggact tttttcggtt tggtgaattt agactcggtg aaagtcacgt acagccacgt ccagcaccag ggcgtgctcg actacgtcga gagcagacgc gacactgtgg aagtgctgaa gcggaagcag cggcacgtgc aaaagttcaa cctccccaaa gggtgcgttg cagacgagga ttcctactgg gtaggatatt gccgcagcgt ctccccgccc tgctacctcg ggcggatcga cgtgcacctt tcgaagggtg ttcctaatga acaggctgtg cctctggaac ccattccgta cgaagaattt gttgaacagt tggtggaaag agagggtatg ggcttttacg acgtcgagga catgttcgat gcggaaaccc ccatgtaccg tctcacgtgg gatccagaaa agtgctttgc ccacttccga tacttcaccg acgacctggc ggcgaccgcg tactactgga ttcagagtcg ccaggccttt cgaatcgacg tgaagaatcc ggaggacaaa ttcgtgagaa aaatgtccgc aatgggggca gactgcggcg gagagggccc ccacgagttc tcagtgacct acaagcggct cgaagacggc tcgtacaagg cgctcttccg cgcgcgcaag ctcaacgact acgaaccccg tcacacggac acgattgaac tgaaagaagc gaaaccaaac ttcccagttc aggactggga ccccgagatg ggcgaagtca tggacttcat tgacttattc ccagaagtcg atgctgttgc tctcgacatg atggctaagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKGVSSRCS ALLRGGCLAL AVLAALERLF TVADNVATAE NVQSGFPPLG VSPLEGTTQV GSSPRRLMSE GLSMMVMQYL LFPEMRESLV DLGAKSMEIL KGGPTYDVLL DYYYDPQNCV VAFDTLYDGT FFGLVNLDSV KVTYSHVQHQ GVLDYVESRR DTVEVLKRKQ RHVQKFNLPK GCVADEDSYW VGYCRSVSPP CYLGRIDVHL SKGVPNEQAV PLEPIPYEEF VEQLVEREGM GFYDVEDMFD AETPMYRLTW DPEKCFAHFR YFTDDLAATA YYWIQSRQAF RIDVKNPEDK FVRKMSAMGA DCGGEGPHEF SVTYKRLEDG SYKALFRARK LNDYEPRHTD TIELKEAKPN FPVQDWDPEM GEVMDFIDLF PEVDAVALDM MAKEQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagggagtc tcatctcggt gctccgccct tctgcggggt ggctgcctcg cgttggcggt gctggcggca ctcgaacggt tgttcactgt tgctgacaac gttgcaactg cggaaaatgt gcaaagcggc tttccaccgc taggcgtctc gccgctcgaa ggcacaactc aggtggggtc gtcgccgcgg cggctgatga gtgagggact atcaatgatg gtgatgcagt atttgctgtt tccggaaatg cgagagtctc tggttgatct cggggcgaag agcatggaaa ttctcaaagg cgggcccacg tacgacgtgc ttttggatta ctactacgac ccgcaaaact gcgtcgtcgc ctttgacacg ctgtacgacg ggactttttt cggtttggtg aatttagact cggtgaaagt cacgtacagc cacgtccagc accagggcgt gctcgactac gtcgagagca gacgcgacac tgtggaagtg ctgaagcgga agcagcggca cgtgcaaaag ttcaacctcc ccaaagggtg cgttgcagac gaggattcct actgggtagg atattgccgc agcgtctccc cgccctgcta cctcgggcgg atcgacgtgc acctttcgaa gggtgttcct aatgaacagg ctgtgcctct ggaacccatt ccgtacgaag aatttgttga acagttggtg gaaagagagg gtatgggctt ttacgacgtc gaggacatgt tcgatgcgga aacccccatg taccgtctca cgtgggatcc agaaaagtgc tttgcccact tccgatactt caccgacgac ctggcggcga ccgcgtacta ctggattcag agtcgccagg cctttcgaat cgacgtgaag aatccggagg acaaattcgt gagaaaaatg tccgcaatgg gggcagactg cggcggagag ggcccccacg agttctcagt gacctacaag cggctcgaag acggctcgta caaggcgctc ttccgcgcgc gcaagctcaa cgactacgaa ccccgtcaca cggacacgat tgaactgaaa gaagcgaaac caaacttccc agttcaggac tgggaccccg agatgggcga agtcatggac ttcattgact tattcccaga agtcgatgct gttgctctcg acatgatggc taaggaacag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa