TogoA.17219.a

unnamed apical complex protein

CENTER ID: TogoA.17219.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.17219.a.A1.GE29387 full length 1 393
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_246940
RefSeq: XP_002367071.1
UniProt: B6KGU8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRMKNPFGF CFGGGKKGRR SDSRKAAQEI PAKQPEEEEE EETQIPLRYP LFDPRAVKTR AEDLGEAPLS RPASIPVEMT TRDHIELPED EEENGFEGEE EPEEEVSEKE APDEIEAPSP REEEAPATPP MKEVEEPHQE REEVEEPPEE REEVEAPPQE REEVEVPPRP APHAEGEKLP TPPGSEQPSR EASQEVQLVN RDPVPVSPPP KAPGQLKTPS PERVPIPEPR EPTPEPSEST ARSPSAASVQ LPEREPAHEV PVFAPPPAGD QPQMSAFEKL ENAREGSVSD SLPTAPHTPR GRFSDDDMAR RLQAKLAELE TLQTLAEVTY YRQKRRTRCR MRPRWVCVEK AIDFYADAHE EFTARVREKM NVHQKKDCWI CRDFDFSDIP VLQ
NT Sequence
atgggacgca tgaagaatcc gttcggtttc tgcttcggcg ggggaaagaa ggggaggcgc agcgacagcc ggaaggcggc tcaggaaatt cctgcgaaac agcctgaaga ggaggaagaa gaagagacac agattcctct tcgctatcct ctcttcgacc ccagagcagt caaaacccgc gccgaagacc tcggcgaggc gccgctctcg agaccggcca gcatccccgt ggaaatgaca actcgggacc acatcgaact ccccgaagac gaagaagaaa acggttttga aggtgaagag gaacctgagg aagaagtttc ggaaaaagaa gctccagatg aaatcgaagc gccttctccc cgtgaggaag aggcacccgc cacacctcca atgaaagaag ttgaggaacc tcatcaggaa cgtgaggaag tggaggaacc tcctgaggaa cgggaagaag tagaggcacc tcctcaggaa cgggaggaag tcgaggtgcc gccgcggcct gcccctcatg cagaaggaga gaagcttccg acaccgccgg ggagtgagca gccttcccga gaagccagtc aggaagttca gttggtcaac cgggacccgg tgcctgtttc tccacctccg aaagcacctg gacaactcaa gacaccttcc ccggagcgcg tgccaattcc agagcctagg gaaccgacac cggagccgtc cgagtcgacg gcgcgttccc cctcggcggc ttctgtccaa ctgccagaga gagaacccgc gcacgaagtg ccagtcttcg cgccgcctcc cgccggcgat cagccgcaaa tgtctgcgtt tgaaaagctg gaaaatgcgc gagaaggcag tgtcagcgac agcctgccga ccgctccgca tacgcctagg ggacgatttt cagacgatga catggctcga agacttcagg cgaaactcgc agaactggag acgctgcaga cgctcgctga ggtcacctac taccgccaaa agcgacgaac acgctgcaga atgcgaccta gatgggtgtg tgtggagaaa gccattgatt tctacgcaga cgctcacgaa gagttcactg cgagagtccg agagaaaatg aacgttcacc agaaaaagga ctgctggata tgcagggact ttgacttctc agacatccca gttcttcagt ga
Details for TogoA.17219.a.A1.GE29387
HARVESTED ON: 5/3/2010
SEQUENCED ON: 5/6/2010
EXPECTED MW: 46kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MGRMKNPFGF CFGGGKKGRR SDSRKAAQEI PAKQPEEEEE EETQIPLRYP LFDPRAVKTR AEDLGEAPLS RPASIPVEMT TRDHIELPED EEENGFEGEE EPEEEVSEKE APDEIEAPSP REEEAPATPP MKEVEEPHQE REEVEEPPEE REEVEAPPQE REEVEVPPRP APHAEGEKLP TPPGSEQPSR EASQEVQLVN RDPVPVSPPP KAPGQLKTPS PERVPIPEPR EPTPEPSEST ARSPSAASVQ LPEREPAHEV PVFAPPPAGD QPQMSAFEKL ENAREGSVSD SLPTAPHTPR GRFSDDDMAR RLQAKLAELE TLQTLAEVTY YRQKRRTRCR MRPRWVCVEK AIDFYADAHE EFTARVREKM NVHQKKDCWI CRDFDFSDIP VLQ
Validated NT Sequence
gggtaccctg gaagctcaga cccagggtcc tggttcgatg ggacgcatga agaatccgtt cggtttctgc ttcggcgggg gaaagaaggg gaggcgcagc gacagccgga aggcggctca ggaaattcct gcgaaacagc ctgaagagga ggaagaagaa gagacacaga ttcctcttcg ctatcctctc ttcgacccca gagcagtcaa aacccgcgcc gaagacctcg gcgaggcgcc gctctcgaga ccggccagca tccccgtgga aatgacaact cgggaccaca tcgaactccc cgaagacgaa gaagaaaacg gttttgaagg tgaagaggaa cctgaggaag aagtttcgga aaaagaagct ccagatgaaa tcgaagcgcc ttctccccgt gaggaagagg cacccgccac acctccaatg aaagaagttg aggaacctca tcaggaacgt gaggaagtgg aggaacctcc tgaggaacgg gaagaagtag aggcacctcc tcaggaacgg gaggaagtcg aggtgccgcc gcggcctgcc cctcatgcag aaggagagaa gcttccgaca ccgccgggga gtgagcagcc ttcccgagaa gccagtcagg aagttcagtt ggtcaaccgg gacccggtgc ctgtttctcc acctccgaaa gcacctggac aactcaagac accttccccg gagcgcgtgc caattccaga gcctagggaa ccgacaccgg agccgtccga gtcgacggcg cgttccccct cggcggcttc tgtccaactg ccagagagag aacccgcgca cgaagtgcca gtcttcgcgc cgcctcccgc cggcgatcag ccgcaaatgt ctgcgtttga aaagctggaa aatgcgcgag aaggcagtgt cagcgacagc ctgccgaccg ctccgcatac gcctagggga cgattttcag acgatgacat ggctcgaaga cttcaggcga aactcgcaga actggagacg ctgcagacgc tcgctgaggt cacctactac cgccaaaagc gacgaacacg ctgcagaatg cgacctagat gggtgtgtgt ggagaaagcc attgatttct acgcagacgc tcacgaagag ttcactgcga gagtccgaga gaaaatgaac gttcaccaga aaaaggactg ctggatatgc agggactttg acttctcaga catcccagtt cttcagta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGRMKNPFG FCFGGGKKGR RSDSRKAAQE IPAKQPEEEE EEETQIPLRY PLFDPRAVKT RAEDLGEAPL SRPASIPVEM TTRDHIELPE DEEENGFEGE EEPEEEVSEK EAPDEIEAPS PREEEAPATP PMKEVEEPHQ EREEVEEPPE EREEVEAPPQ EREEVEVPPR PAPHAEGEKL PTPPGSEQPS REASQEVQLV NRDPVPVSPP PKAPGQLKTP SPERVPIPEP REPTPEPSES TARSPSAASV QLPEREPAHE VPVFAPPPAG DQPQMSAFEK LENAREGSVS DSLPTAPHTP RGRFSDDDMA RRLQAKLAEL ETLQTLAEVT YYRQKRRTRC RMRPRWVCVE KAIDFYADAH EEFTARVREK MNVHQKKDCW ICRDFDFSDI PVLQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggacgcatg aagaatccgt tcggtttctg cttcggcggg ggaaagaagg ggaggcgcag cgacagccgg aaggcggctc aggaaattcc tgcgaaacag cctgaagagg aggaagaaga agagacacag attcctcttc gctatcctct cttcgacccc agagcagtca aaacccgcgc cgaagacctc ggcgaggcgc cgctctcgag accggccagc atccccgtgg aaatgacaac tcgggaccac atcgaactcc ccgaagacga agaagaaaac ggttttgaag gtgaagagga acctgaggaa gaagtttcgg aaaaagaagc tccagatgaa atcgaagcgc cttctccccg tgaggaagag gcacccgcca cacctccaat gaaagaagtt gaggaacctc atcaggaacg tgaggaagtg gaggaacctc ctgaggaacg ggaagaagta gaggcacctc ctcaggaacg ggaggaagtc gaggtgccgc cgcggcctgc ccctcatgca gaaggagaga agcttccgac accgccgggg agtgagcagc cttcccgaga agccagtcag gaagttcagt tggtcaaccg ggacccggtg cctgtttctc cacctccgaa agcacctgga caactcaaga caccttcccc ggagcgcgtg ccaattccag agcctaggga accgacaccg gagccgtccg agtcgacggc gcgttccccc tcggcggctt ctgtccaact gccagagaga gaacccgcgc acgaagtgcc agtcttcgcg ccgcctcccg ccggcgatca gccgcaaatg tctgcgtttg aaaagctgga aaatgcgcga gaaggcagtg tcagcgacag cctgccgacc gctccgcata cgcctagggg acgattttca gacgatgaca tggctcgaag acttcaggcg aaactcgcag aactggagac gctgcagacg ctcgctgagg tcacctacta ccgccaaaag cgacgaacac gctgcagaat gcgacctaga tgggtgtgtg tggagaaagc cattgatttc tacgcagacg ctcacgaaga gttcactgcg agagtccgag agaaaatgaa cgttcaccag aaaaaggact gctggatatg cagggacttt gacttctcag acatcccagt tcttcagaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa