TogoA.17198.a

unnamed apical complex protein

CENTER ID: TogoA.17198.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.17198.a.A2.GE29365 N-terminal domain 263 546
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_272390
OrthoMCL: OG5_240313
RefSeq: XP_002365872.1
UniProt: B6KDE9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEEERQASS PRRRCSILKL FLCLLQFLFC VSTPTLLLSL AALYLVLPLF GDSPNAGFLG FVFADASRLP ASGVFSSFPG LYYAQRQEAS HPRPSHASHA ASLSTVSSSL SSVEPSDGCK RLSTAFSIRR LPPSSDARSS SLSHSSRPLS SAPVAFAGPR RADPLSVEQK GSGDVDTPEA ALQLPSLPPH YTIVGFRVQG SQVLPSRLVR QLEKELLSKH VSPASTSSAS STSSSNAPRV SRRSASPSPG SPAASSKSSA LSAEENEALI RTFVDIVNAW YLENGYLFAH LVPRPHFLPS SVPVSARGAP GVSAGTETTE KPVPAFLISF DCEEPPTANP PLHLAFFARQ RPPKTAPDSP RTQLDTAEKA AQSTSVEHDA HRCEELVETQ GTLNPELLAH HLGLVPGKPF KWDAQRWHRV ATEADLFADA HATAAVLPGD GGIRVEVAAV EKPACSLRPG LSFSSALRDV EGQLLLEHRN LFGTAARGSV ALRLSPSLDF LPGQKQTTDD AVSLPPSRAQ SSSLCAELLF NSLSRATDRE SVRLLASASS SYSPPPLLPS SPSRSNPSLP SSSLLSRILS APPPTPESPT SVSGVSAPLP ATPPQTPARL LVPAGHFRVG FAASGERRLG AAGTMWGEAT IERRKGLYVN LGLAGSASRQ GSDSSRLPSS LAEASDTSHV GEKRNEDAPH RKESQSWGDK VRNALGMLQP PRFAPTTTTT SVAMVSADDV ASLLPSRERG SVLTDPSVAH DVAKVAGGVR FQFSESSRQF GLPPTRLAPT WGFEASSFAG VYTPYTAADA AAAFAPLTSS GSASTRPGSR PSRVSRDRPL PPLPGLAGFS PPRPHVGTAR SFSLSSLVPS RVSAFAAKLV PRPLRDESRF SQRCREVKET VSAALASVSP FVASRAWAAG VWTRTVALAN LPFAPVLFAS SFPSASPSLY SSCPPLPYCG ASLSFASRVF PFSSLFLSRS SYASSTSQGR LRSLLLRPLI SLVSPFDFSQ LAVRTRLNID LLLPPRLLLP ILASCLSPAS SSPSCSARSS PPLPRQMWQD VACRGRALWA QVSSHVSAYL SSCSSALSSA EAVNASVASS LPSSSSLCVL PRLSLCAFPV IHPSTASPAV SLPSTTDPLS PPREAIETSL LSEPAAALPF SSSSTSPMSS SVASSACSAP AKSSEPAGAA GKAHAAPAVV SSLPSHADRR RFLASSLRAA AARLPPWEML KLSGQKGNRL RAWGAEPVGI FEAVADGAIE LSLPIVARLP LGGGTARLPL KLMEVAFFFD HSVGVSPFLL PGTSSSSPSP AAAAASALGA TAAAIAVGDS SLGSSTLTSA AEEGSGVGGS RPLRFLAQAK QRLLRRGHDI EETLLNAVDR VREKTGWSVC HWPSVGVCVR LALLSVTFAR PIAFRNGGMS LLPGRFFVGV ADNSF
NT Sequence
atggcggagg aagaaagaca ggcatcgtcg ccacggcggc ggtgttcaat cttgaagctc tttctctgtc tccttcagtt ccttttctgc gtgtcgactc ccacactgct tctctctctt gccgcattgt atctcgttct cccgctcttc ggcgactctc cgaatgccgg gttcctcggc ttcgtctttg cggatgcgtc tcgtcttcca gcctcggggg tcttctccag tttccctggt ctctactacg cacagcgaca ggaagcgtcg cacccccgcc cctctcacgc atcgcacgct gcctctctct ctaccgtctc ctctagcctc tcctcggtcg aaccgagcga cggctgcaag cgcctctcaa ctgctttctc gattcgtcgt cttcctccgt cctctgatgc ccgctcttct tctctctccc actcatcgcg ccctttgtct tctgctcctg tcgccttcgc gggaccgcgt cgtgcagacc ctctgtctgt tgagcagaag gggagtggcg atgtcgacac ccccgaagcg gctcttcagc ttccctcgct gccgccgcac tacacgatcg ttggcttcag agtccaaggc tcccaagtgc ttccctcgcg ccttgtccga cagcttgaga aggaacttct ctcgaaacac gtcagtcccg cctcgacgtc ttccgcctcg tccacgtcct cttccaacgc ccctcgtgtg tctcgtcggt ctgcttcgcc ttctccaggt tctcccgccg cttcatccaa gtcctctgca ttgtcggcag aggaaaatga agccttgatc cgtaccttcg tggatatcgt gaacgcgtgg tacctcgaaa atggctattt gtttgcgcac ttggttccgc gtccccactt ccttccttcc agcgttccag tcagcgcccg tggagccccc ggtgtctcgg ctggcactga aacgacggag aagcctgtcc ccgcgttcct catttcgttc gactgcgagg agcctccaac tgcaaatccc cctctccacc tcgccttctt cgcgcgacag agacccccga agacagcgcc cgactcgccg cgaacacagc tagacactgc cgagaaggcc gcgcaatcga cctccgtgga acacgacgca caccgctgcg aggaactcgt cgaaacacag ggcaccctga acccagagct cctcgcccat cacttgggcc tcgtcccggg caagccgttc aagtgggatg cccagcgctg gcaccgagtg gcaactgaag cggatctttt cgcggacgca cacgcgactg ccgcggtgct tcctggagac gggggcatcc gcgtggaagt cgccgccgta gaaaaacctg cttgttctct tcggccaggc ctctcgtttt cgtctgcgct ccgcgacgtc gagggacagc ttctgctgga acatcgaaat cttttcggca ctgccgcccg cgggagcgtc gccctgcggc tctcgccctc gctcgatttt ctgccaggac agaaacagac gacagacgac gcagtttcac ttcctccctc cagagcccaa agctcttcgc tctgtgctga actcctcttc aactctctct cgcgagcaac agaccgggag tctgtccggt tgctggcctc ggcgtcgtct tcgtattcgc ctcctcccct tttgccatcg tcgccttctc ggtcgaatcc cagtcttccg tcgtcttctc tgttgtctcg cattctctcc gcgccgcctc cgacgcccga gtcgccgacg tctgtctctg gtgtatctgc acctctgccc gccactccgc cgcagacgcc ggcgcgtctc ctcgttcccg caggtcattt tcgtgtgggc tttgcagcct caggagaaag gcgcttgggc gcggcgggga cgatgtgggg tgaggcgact atcgagcggc gcaagggact gtacgtcaac ctgggactcg cgggcagcgc ctcgcgccag ggcagcgact cgtcgaggct ccccagttcg ttggcggaag cgtctgacac gtcacatgtt ggtgagaaga gaaacgaaga cgcgccgcat cgcaaggaga gccaaagctg gggcgacaag gtacgaaacg ctctaggcat gctgcagcct cctcggtttg caccgacaac gactacaacc tccgtcgcga tggtctctgc cgatgacgtc gcttctcttc ttccctcgcg ggaacgcggc tctgtgctca cggatccctc ggtcgcccac gatgtcgcca aggtcgcagg aggtgtgcgc ttccagttct ctgaatcgtc gcgtcagttt ggcctgcctc ccacgaggtt ggcgccgacc tggggcttcg aggcgtcgtc gttcgcaggc gtctatacac cgtacacggc tgccgacgcg gcagccgcgt tcgcgcctct tacgtcttct ggtagcgcct caacacgccc tggatctcgg ccgtcgcggg tgtcccggga tcgtcctctt ccaccgcttc caggtctcgc ggggttctca ccgcctcggc ctcacgtcgg taccgcgcgg tccttttcgc tgtcctcctt ggtgccttct cgcgtgtctg cgtttgccgc caagctggtg ccgcgaccgc tgagagacga gagccgcttc tcacagagat gccgggaggt gaaggagacg gtttctgcgg ccctcgcctc tgtctcgcct ttcgtcgcct cccgggcgtg ggcggcgggt gtgtggacgc gaactgttgc tctcgctaac ctcccgtttg cgcctgttct ctttgcttca tcttttccgt ctgcgtctcc gtctctgtac tcctcttgtc cgccgcttcc gtattgtgga gcgtccctgt cgttcgcgtc ccgtgtcttt ccgttttcgt ctctctttct gtcccgctcc tcctacgctt cttccacctc tcaaggaagg ctccgaagcc ttctcttacg tcctctgatt tctctcgtct cgcccttcga tttctcccaa ctcgctgttc ggactcgcct caacatcgac cttctcctgc ctccacgtct ccttcttcct atcctcgcca gctgtctctc tcccgcgtcg tcttctccgt cgtgttctgc tcgttcgtct cctccgcttc ctcgtcagat gtggcaagat gtcgcatgtc gcgggcgtgc actttgggct caggtatcct cgcatgtttc tgcgtatctt tcctcttgtt cttctgcgct ttcgtctgcc gaggccgtca acgcgtctgt cgcgtcttcc cttccttctt cgtcttccct ttgcgtcctc cctcgtctct ccctctgcgc ctttcctgtc attcacccgt ccacggcgtc gcccgctgtt tcgctcccgt cgaccactga ccctctttct cccccccgcg aggccatcga aacgtcgcta ctctccgagc ctgccgccgc gctccccttt tcttcctcgt caacctctcc catgtcttct tcggttgctt cgtcggcttg ctccgcgcca gcgaagtctt cagagcctgc gggcgcagcg gggaaggcgc atgcagctcc tgccgttgtc tcgtccctcc cctcgcacgc agacagacgt cggttcctcg caagctcgct acgagcagct gccgcgcggc tgcctccctg ggagatgctg aaactttcag ggcagaaggg caacagactg cgcgcctggg gtgcagagcc cgtgggaatc tttgaggcgg tcgcggatgg cgcgatcgaa ctcagcctgc ccatcgtcgc cagactacca ctcggaggcg gcaccgctcg tcttcccttg aagctgatgg aagtcgcctt ctttttcgac cacagcgtgg gggtctcgcc ctttcttctt cctggcacgt cgtcgtcgtc cccgagccca gccgccgccg ccgctagcgc cttaggcgcc acggcagccg cgatcgcggt cggagacagc tcgttgggct cgagcaccct gaccagcgcg gcagaggagg ggtctggcgt cggcggcagt cggcccctgc ggtttctcgc ccaagcgaag cagagacttt taagacgagg ccacgacatc gaggagacgc ttttgaatgc agtcgacaga gtgcgcgaga aaacaggctg gagtgtttgc cactggccct cggtgggcgt ctgcgttcgc ctagcgttgc tgagcgtcac cttcgcaagg cctatcgcat tcagaaacgg cgggatgtct cttctccctg ggcggttctt tgtaggcgtg gccgacaaca gcttctag
Details for TogoA.17198.a.A2.GE29365
HARVESTED ON: 5/3/2010
SEQUENCED ON: 5/6/2010
EXPECTED MW: 33kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAEENEALI RTFVDIVNAW YLENGYLFAH LVPRPHFLPS SVPVSARGAP GVSAGTETTE RPVPAFLISF DCEEPPTANP PLHLAFFARQ RPPKTAPDSP RTQLDTAEKA AQSTSVEHDA HRCEELVETQ GTLNPELLAH HLGLVPGKPF KWDAQRWHRV ATEADLFADA HATAAVLPGD GGIRVEVAAV EKPACSLRPG LSFSSALRDV EGQLLLEHRN LFGTAARGSV ALRLSPSLDF LPGQKQTTDD AVSLPPSRAQ SSSLCAELLF NSLSRATDRE SVRLLA
Validated NT Sequence
ttattaggcc agcaaccgga cagactcccg gtctgttgct cgcgagagag agttgaagag gagttcagca cagagcgaag agctttgggc tctggaggga ggaagtgaaa ctgcgtcgtc tgtcgtctgt ttctgtcctg gcagaaaatc gagcgagggc gagagccgca gggcgacgct cccgcgggcg gcagtgccga aaagatttcg atgttccagc agaagctgtc cctcgacgtc gcggagcgca gacgaaaacg agaggcctgg ccgaagagaa caagcaggtt tttctacggc ggcgacttcc acgcggatgc ccccgtctcc aggaagcacc gcggcagtcg cgtgtgcgtc cgcgaaaaga tccgcttcag ttgccactcg gtgccagcgc tgggcatccc acttgaacgg cttgcccggg acgaggccca agtgatgggc gaggagctct gggttcaggg tgccctgtgt ttcgacgagt tcctcgcagc ggtgtgcgtc gtgttccacg gaggtcgatt gcgcggcctt ctcggcagtg tctagctgtg ttcgcggcga gtcgggcgct gtcttcgggg gtctctgtcg cgcgaagaag gcgaggtgga gagggggatt tgcagttgga ggctcctcgc agtcgaacga aatgaggaac gcggggacag gcctctccgt cgtttcagtg ccagccgaga caccgggggc tccacgggcg ctgactggaa cgctggaagg aaggaagtgg ggacgcggaa ccaagtgcgc aaacaaatag ccattttcga ggtaccacgc gttcacgata tccacgaagg tacggatcaa ggcttcattt tcctctgcca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEENEALI RTFVDIVNAW YLENGYLFAH LVPRPHFLPS SVPVSARGAP GVSAGTETTE KPVPAFLISF DCEEPPTANP PLHLAFFARQ RPPKTAPDSP RTQLDTAEKA AQSTSVEHDA HRCEELVETQ GTLNPELLAH HLGLVPGKPF KWDAQRWHRV ATEADLFADA HATAAVLPGD GGIRVEVAAV EKPACSLRPG LSFSSALRDV EGQLLLEHRN LFGTAARGSV ALRLSPSLDF LPGQKQTTDD AVSLPPSRAQ SSSLCAELLF NSLSRATDRE SVRLLA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagaggaa aatgaagcct tgatccgtac cttcgtggat atcgtgaacg cgtggtacct cgaaaatggc tatttgtttg cgcacttggt tccgcgtccc cacttccttc cttccagcgt tccagtcagc gcccgtggag cccccggtgt ctcggctggc actgaaacga cggagaagcc tgtccccgcg ttcctcattt cgttcgactg cgaggagcct ccaactgcaa atccccctct ccacctcgcc ttcttcgcgc gacagagacc cccgaagaca gcgcccgact cgccgcgaac acagctagac actgccgaga aggccgcgca atcgacctcc gtggaacacg acgcacaccg ctgcgaggaa ctcgtcgaaa cacagggcac cctgaaccca gagctcctcg cccatcactt gggcctcgtc ccgggcaagc cgttcaagtg ggatgcccag cgctggcacc gagtggcaac tgaagcggat cttttcgcgg acgcacacgc gactgccgcg gtgcttcctg gagacggggg catccgcgtg gaagtcgccg ccgtagaaaa acctgcttgt tctcttcggc caggcctctc gttttcgtct gcgctccgcg acgtcgaggg acagcttctg ctggaacatc gaaatctttt cggcactgcc gcccgcggga gcgtcgccct gcggctctcg ccctcgctcg attttctgcc aggacagaaa cagacgacag acgacgcagt ttcacttcct ccctccagag cccaaagctc ttcgctctgt gctgaactcc tcttcaactc tctctcgcga gcaacagacc gggagtctgt ccggttgctg gccaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa