TogoA.17194.a

unnamed apical complex protein

CENTER ID: TogoA.17194.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.17194.a.A3.GE30581 SMC-like domain 1 474
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_239300
OrthoMCL: OG5_163290
RefSeq: XP_002366570.1
UniProt: B6KGH0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADLTLEPRG AGASRYLMTD LEDLDSSEGN SLVPYSRLGK SGNTDANLYS TPDMTTRYPG SRGLLRDDSR GSGGLDDPMG LPMRSLSPKD LGGRGGPRLS PAELKAQVRL RNMCGKPLRP DDVLVTRILT GEKSYWEALD EMRNLCASLT VENEKLKTGN AKATTKALEV EQKSRAEIRR LQTLQRVTRD ELEMKLQDLT AENVRLRQSR DVERALQEQK VHMQKQIERL KQDSEEKGRL AAQRAREIHH LKEETQRSTD EAEALKGRVE SLQAKLAEAI SQKNLLLIRV KKDIRDIQDS AQQAREAVLA EKARSEQQAA EVEKHAQMQL QLHERKIRDL QQTVASEESQ GEYYRRKYEE ASKQLAKLKT QFDSTQNKTE TDIALRERKL QELSESLNAA QKARDRSAQE LNAMRSKYNI LRGELEKAEQ RRNHAEQEAI RAGVCASESK RDWTAKRKQG RGNAQVETMD VGRSTLEAKV SELQLERSGL MEKLQLAEKE VSELREAKNK IKASLDLAYQ KEQFRLLEEN SRIGTDLKVA QQQLDTVTRQ KEQLQTDAER LRGAEEELRE MQVDFLKMKT TLQLVQGRPC DNAKVVADGV VNALRAYAEL KKATETQEQS MASLSQQNQA LSQKANEAAT LTREMLTLES ENRSLNEHIA SLTAELRKIG DQQTALQLEK EAQDEAFRQQ IERVQKEAEA RRRELDHQLE QAKERHRQET TKLTAELDRV KKLHDAEKKR TSELQVQVED VKAEATQHTE GLEKRYQEQL RERNAQIEAL SSEREEFAAE TRAEIQQLKK THADALKAAR ADRDKVLAER ESHYQQQLEE MRTRQEQHAA SQHHQRQELQ QRLQQLELSF QETEANLRRE VDAKAADVRS LQGQKEKLER ELGAVKERLE DEQRSADRNL ERVKRERQQD VERLQKELDD ATRLAAEAER TAAEKIQSLE TDVKNTRDQL KKANAGSEEK ESLLEASKTR YADLENKHND VVQKFNDLSE ENKKLKKDLS AAADDKEAKE KQIKKLTDAQ ARDQQEIAAI KQKEREAIAA GDRLNAEIEK LKAEHRSAEL KLKNDIDAVT TDKEAVEAKL AQVQKMHKEE AEALNSRIAQ LTKEKADLEK AQRNLEEEKA ALEQEKRKAA SEAKVKQDLL EQKIAELQKQ NQNLENGLRA AKAAGAAAAA RPAPGAPGQA PPAAQAPQAG APPAEEGGLF SWWGGGAAEA PPEAPASSAG APAEAAPAPA AEAESSSWGW W
NT Sequence
atggcagatc tgacactcga gccccggggc gcgggtgcgt cgcgttatct gatgacagat ctagaggatt tggactcgtc tgaagggaac agcttagttc cctattcgcg gttgggcaag tcggggaaca cggatgcgaa tctgtactca actccagaca tgacgactcg gtacccgggg tcgcggggcc tgctgcggga cgactcgcgg ggctctgggg ggctagatga ccctatgggg ttgccgatgc ggtccctgtc ccccaaggat ctggggggcc ggggcggccc gcgcctgtct cccgccgagc tgaaggccca ggtgaggctc cgtaacatgt gcgggaaacc tctgcgccca gacgacgtgc tcgtgacgcg gatcttgacg ggggaaaaga gctactggga ggcactggac gagatgcgga acctgtgtgc aagtctgaca gtggagaacg agaagctcaa gaccggcaac gcgaaggcga cgaccaaggc gctggaagtc gagcagaaga gccgcgccga gatccggaga ctacagactc tgcagcgcgt gacccgggac gaactggaga tgaagctaca agacttgacg gcggaaaacg ttcgactgcg gcagtcgcga gacgtcgaga gggcgctcca ggagcagaag gttcacatgc agaagcaaat agaacggttg aaacaggaca gtgaagagaa gggacgcttg gcggcgcagc gcgctcgcga gattcaccac ctgaaggagg agacgcagcg tagcacggat gaggctgaag cgttgaaggg ccgcgtcgag agcctccagg cgaagctcgc tgaggcgatc tcgcagaaga acctcctgct gattcgcgtg aagaaagaca ttcgagacat ccaggactcg gcgcagcagg cgcgggaggc cgtgctcgct gagaaggcgc gcagcgagca gcaagccgcc gaagtcgaaa aacatgcgca gatgcagctc cagctgcacg agcgaaagat ccgcgatctc cagcaaacgg tggcatcgga ggagagccag ggcgagtatt acagacgaaa gtacgaggag gcatccaagc agctggccaa gctgaagaca cagttcgaca gcacgcaaaa caagacagag acagacatcg cattgcgaga acggaagctc caagaactga gcgagagcct gaacgctgcg cagaaggcca gagaccgcag tgcgcaggaa ctcaatgcta tgcgctccaa gtacaacatt ctcaggggag aactcgagaa ggcggaacag agacgaaacc acgccgaaca ggaggccatt cgagccgggg tatgcgccag cgaatcgaag cgagattgga cggcgaaacg aaagcagggg aggggaaacg ctcaagtcga gacgatggac gtcggccgga gcacgctgga ggcaaaagtg agtgagcttc agctcgaacg atctggcctc atggagaagc ttcaactcgc agaaaaggag gtgtccgaac tgcgagaagc gaagaacaaa atcaaagctt ctctggacct cgcgtaccag aaggaacagt tcagactgtt ggaggagaac agccgcatcg gcaccgactt gaaagtcgcg cagcaacagc tcgacacggt cacgcgacaa aaggagcagc ttcagaccga cgcagagcgc ctgcggggag ccgaggaaga actgcgagag atgcaagttg acttcctcaa aatgaaaacg acactgcaac tcgtccaggg gcgcccctgc gacaacgcta aggtcgttgc cgatggcgtt gtcaacgccc ttcgagcgta cgcggaactc aaaaaggcaa ctgaaactca ggagcagtcg atggcatctc tctctcagca gaaccaagcg ttgagtcaga aggcgaatga ggctgcgacg ctgacgcgag aaatgcttac acttgagtct gagaatcggt ctctgaacga acacattgcg agtctgacag ccgaactgcg gaagatcgga gaccagcaga cggcgctgca gttggagaag gaggcgcagg acgaagcctt tcgccagcag atcgaacgcg tgcagaaaga agctgaagcg cgtcggcgag aactggacca ccagctcgaa caggcgaagg agaggcatcg tcaggagacc acgaagctga cggcagagtt ggatcgtgtg aagaaactgc acgacgccga gaagaagcgc accagcgagc tgcaggtgca ggtggaagat gtgaaggcgg aggcgactca acacaccgag ggtctggaga agaggtacca agagcagctc cgagagcgga acgcgcagat cgaggccttg agctctgagc gggaagagtt cgctgcggag acgagggctg agattcagca gctgaagaaa acacatgcag atgcgttgaa ggcggcccgg gcagaccggg acaaagtcct ggctgagcga gaaagccact accagcagca actcgaagag atgcgcacgc gccaggagca acatgctgcg tcgcagcatc accaaaggca agagctgcag cagcgcctgc agcagttgga actttcgttc caagaaaccg aagcgaatct gcgtcgcgag gtggacgcga aggccgccga tgtgcggtcg ctgcagggac agaaggagaa gctggagcgc gagctgggcg ctgtgaagga gcgcctggag gacgaacagc gaagcgcaga taggaacctg gagcgcgtca agcgcgagag acaacaggac gtcgagagac tgcaaaagga actggacgac gcgacgcgac tcgccgccga agctgaacgc acagctgcag agaagatcca gagtctcgag acagacgtga agaacactcg agaccagttg aagaaggcaa atgcaggcag cgaagagaaa gaaagccttt tggaggcttc aaagaccagg tacgcagacc tcgagaacaa acacaacgac gtggtgcaga agttcaatga cctctcggag gaaaacaaga agctcaagaa ggacttgagc gcggcagctg acgacaaaga ggcgaaggaa aagcaaatca agaaactgac ggacgcacag gctcgagacc aacaggaaat tgcggccatc aaacaaaagg agagagaagc tatcgccgcg ggagaccgcc taaacgcaga aatcgaaaaa ctgaaggcgg aacaccgctc tgcagagttg aagctcaaaa atgacatcga tgctgtcacc acggataaag aggcagtcga ggcgaagctg gcgcaagttc aaaagatgca caaggaagaa gctgaggcgc tgaatagtcg catcgcccag ctgacaaagg agaaagcgga cctcgagaag gcccagcgga acctggagga ggagaaagct gccctagaac aggaaaagcg aaaggctgca agtgaagcaa aggtgaagca agacctcctt gagcaaaaga tcgccgaact tcagaaacaa aatcaaaacc tcgagaatgg gctgagagcg gcgaaggccg ctggagcagc agctgccgcg aggcctgccc ccggcgcgcc aggtcaggcc ccgcccgcag ctcaggcacc acaagccggc gcacccccgg ctgaagaagg aggccttttc tcgtggtggg gaggcggagc ggcagaagct ccaccagagg cgcctgccag tagcgccggc gcaccagccg aggcagcacc cgcgccagct gccgaggctg agagttccag ttggggctgg tggtaa
Details for TogoA.17194.a.A3.GE30581
HARVESTED ON: 8/26/2010
SEQUENCED ON: 3/4/2011
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 94
Validated AA Sequence
MGTLEAQTQG PGSMADLTLE PRGAGASRYL MTDLEDLDSS EGNSLVPYSR LGKSGNTDAN LYSTPDMTTR YPGSRGLLRD DSRGSGGLDD PMGLPMRSLS PKDLGGRGGP RLSPAELKAQ VRLRNMCGKP LRPDDVLVTR ILTGEKSYWE ALDEMRNLCA SLTVENEKLK TGNAKATTKA LEVEQKSRAE IRRLQTLQRV TRDELEMKLQ DLTAENVRLR QSRDVERALQ EQKVHMQKQI ERLKQDSEEK GRLAAQRARE IHHXKEETQR STDEAEALKG RVESLQAKLA EAISQKNLLL IRVKKDIRDI QDSAQQAREA VLAEKARSEQ QAAEVEKHAQ MQLQLHERKI RDLQQTVASE ESQGEYYRRK YEEASKQLAK LKTQFDSTQN KTETDIALRE RKLQELSESL NAAQKARDRS AQELNAMRSK YNILRGELEK AEQRRNHAEQ EAIRAGAQVE TMDVGRS
Validated NT Sequence
atatgggtac cctggaagct cagacccagg gtcctggttc gatggcagat ctgacactcg agccccgggg cgcgggtgcg tcgcgttatc tgatgacaga tctagaggat ttggactcgt ctgaagggaa cagcttagtt ccctattcgc ggttgggcaa gtcggggaac acggatgcga atctgtactc aactccagac atgacgactc ggtacccggg gtcgcggggc ctgctgcggg acgactcgcg gggctccggg gggctagatg acccgatggg gttgccgatg cggtccctgt cccccaagga tctggggggc cggggcggcc cgcgcctgtc tcccgccgag ctgaaggccc aggtgaggct ccgtaacatg tgcgggaaac ctctgcgccc agacgacgtg ctcgtgacgc ggatcttgac gggggaaaag agctactggg aggcactgga cgagatgcgg aacctgtgtg caagtctgac agtggagaac gagaagctca agaccggcaa cgcgaaggcg acgaccaagg cgctggaagt cgagcagaag agccgcgccg agatccggag actacagact ctgcagcgtg tgacccggga cgaactggag atgaagctac aagacttgac ggcggaaaac gtccgactgc ggcagtcgcg agacgtcgag agggcgctcc aggagcagaa ggttcacatg cagaagcaga tagaacggtt gaaacaggac agtgaagaga agggacgctt ggcggcgcag cgcgctcgcg agattcacca cntgaaggag gagactcagc gtagcacgga tgaggctgaa gcgttgaagg gccgcgtcga gagcctccag gcgaagctcg ctgaggcgat ctcgcagaag aacctcctgc tgattcgcgt gaagaaagac attcgagaca tccaggactc ggcgcagcag gcgcgggagg ccgtgctcgc tgagaaggcg cgcagcgagc agcaagccgc cgaagtcgaa aaacatgcgc agatgcagct ccagctgcac gagcgaaaga tccgcgatct ccagcaaacg gtggcatcgg aggagagcca gggcgagtat tacagacgaa agtacgagga ggcgtccaag cagctggcca agctgaagac acagttcgac agcacgcaaa ataagacaga gacagacatc gcattgcgag aacggaagct ccaagaactg agcgagagcc tgaacgctgc gcagaaggcc agagaccgca gtgcgcagga actcaatgct atgcgctcca agtacaacat tctcagggga gaactcgaga aggcggaaca gagacgaaac cacgccgaac aggaggccat tcgagccggg gctcaagtcg agacgatgga cgtcggccgg agcta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADLTLEPR GAGASRYLMT DLEDLDSSEG NSLVPYSRLG KSGNTDANLY STPDMTTRYP GSRGLLRDDS RGSGGLDDPM GLPMRSLSPK DLGGRGGPRL SPAELKAQVR LRNMCGKPLR PDDVLVTRIL TGEKSYWEAL DEMRNLCASL TVENEKLKTG NAKATTKALE VEQKSRAEIR RLQTLQRVTR DELEMKLQDL TAENVRLRQS RDVERALQEQ KVHMQKQIER LKQDSEEKGR LAAQRAREIH HLKEETQRST DEAEALKGRV ESLQAKLAEA ISQKNLLLIR VKKDIRDIQD SAQQAREAVL AEKARSEQQA AEVEKHAQMQ LQLHERKIRD LQQTVASEES QGEYYRRKYE EASKQLAKLK TQFDSTQNKT ETDIALRERK LQELSESLNA AQKARDRSAQ ELNAMRSKYN ILRGELEKAE QRRNHAEQEA IRAGVCASES KRDWTAKRKQ GRGNAQVETM DVGRS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagatctg acactcgagc cccggggcgc gggtgcgtcg cgttatctga tgacagatct agaggatttg gactcgtctg aagggaacag cttagttccc tattcgcggt tgggcaagtc ggggaacacg gatgcgaatc tgtactcaac tccagacatg acgactcggt acccggggtc gcggggcctg ctgcgggacg actcgcgggg ctctgggggg ctagatgacc ctatggggtt gccgatgcgg tccctgtccc ccaaggatct ggggggccgg ggcggcccgc gcctgtctcc cgccgagctg aaggcccagg tgaggctccg taacatgtgc gggaaacctc tgcgcccaga cgacgtgctc gtgacgcgga tcttgacggg ggaaaagagc tactgggagg cactggacga gatgcggaac ctgtgtgcaa gtctgacagt ggagaacgag aagctcaaga ccggcaacgc gaaggcgacg accaaggcgc tggaagtcga gcagaagagc cgcgccgaga tccggagact acagactctg cagcgcgtga cccgggacga actggagatg aagctacaag acttgacggc ggaaaacgtt cgactgcggc agtcgcgaga cgtcgagagg gcgctccagg agcagaaggt tcacatgcag aagcaaatag aacggttgaa acaggacagt gaagagaagg gacgcttggc ggcgcagcgc gctcgcgaga ttcaccacct gaaggaggag acgcagcgta gcacggatga ggctgaagcg ttgaagggcc gcgtcgagag cctccaggcg aagctcgctg aggcgatctc gcagaagaac ctcctgctga ttcgcgtgaa gaaagacatt cgagacatcc aggactcggc gcagcaggcg cgggaggccg tgctcgctga gaaggcgcgc agcgagcagc aagccgccga agtcgaaaaa catgcgcaga tgcagctcca gctgcacgag cgaaagatcc gcgatctcca gcaaacggtg gcatcggagg agagccaggg cgagtattac agacgaaagt acgaggaggc atccaagcag ctggccaagc tgaagacaca gttcgacagc acgcaaaaca agacagagac agacatcgca ttgcgagaac ggaagctcca agaactgagc gagagcctga acgctgcgca gaaggccaga gaccgcagtg cgcaggaact caatgctatg cgctccaagt acaacattct caggggagaa ctcgagaagg cggaacagag acgaaaccac gccgaacagg aggccattcg agccggggta tgcgccagcg aatcgaagcg agattggacg gcgaaacgaa agcaggggag gggaaacgct caagtcgaga cgatggacgt cggccggagc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa