TogoA.17189.a

TgDIP13

CENTER ID:	TogoA.17189.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.17189.a.A1.GE29357	full length		1	248

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_295450
OrthoMCL:	OG5_130151
RefSeq:	XP_002371494.1
UniProt:	B6KUH1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSTTNSNLH NYNHELVACI DDLRRKREEV HRQIIKVGAI PGPAWTRSFR LSKRMYHILV FFISGLIRVK RTALGAVLLP QCTNARVAPW AVSVCHSSRK STAARSLESA HLRQLNQCIF KDVTIFFTFL CMSQEEEDRA KVQKEIALLQ ERLQKLNDSI TRKIQARNEY DKTILETEAA YTKILESAQT LLHVLKRESV HLGRKTSGDR AELPGIGITG VGVDYSPNSV SELSHRRAEK GKPTAGAG

NT Sequence

atgacgagca caacgaactc caacttacac aactacaacc acgagctcgt tgcgtgcatt gatgacctcc gccgcaagcg cgaagaggtc caccgccaga tcataaaagt aggcgcaatt cctggtccag cgtggacaag gtcatttcga ctgtctaagc gaatgtatca tattcttgtt ttctttataa gtggtctcat tcgtgtgaaa cgaacagctc tgggagcggt cttgttgcct cagtgcacaa acgcccgcgt cgctccttgg gctgtctctg tttgtcattc atcccgtaag tcaactgcgg cacgctctct ggagtctgcc catctcagac aactgaatca gtgcatcttc aaagatgtca cgattttttt cacgtttctg tgcatgtctc aggaagagga ggatagagcg aaagtgcaaa aggagatcgc gcttctgcag gaacggcttc agaagctcaa tgatagtata acacgcaaga tccaagcaag gaacgagtac gacaaaacca tcttggagac agaagcagcg tatacaaaga ttttagaatc cgcgcaaacg ctgctgcacg tactcaaacg cgagtcggtg catctgggca gaaaaacgtc tggcgaccgg gcggaattgc ctggtattgg catcacggga gttggtgtgg actattcccc aaatagcgtg tcggagctgt cgcatcgacg ggcggagaaa gggaaaccca cagctggcgc aggatga

Details for TogoA.17189.a.A1.GE29357

HARVESTED ON:	data unavailable
SEQUENCED ON:	5/6/2010
EXPECTED MW:	30kDa
OBSERVED MW:	19kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	63

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTSTTNSNL HNYNHELVAC IDDLRRKREE VHRQIIKEEE DRAKVQKEIA LLQERLQKLN DSITRKIQAR NEYDKTILET EAAYTKILES AQTLLHVLKR ESVHLGRKTS GDRAELPGIG ITGVGVDYSP NSVSELSHRR AEKGKPTAGA G

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacga gcacaacgaa ctccaactta cacaactaca accacgagct cgttgcgtgc attgatgacc tccgccgcaa gcgcgaagag gtccaccgcc agatcataaa agaagaggag gatagagcga aagtgcaaaa ggagatcgcg cttctgcagg aacggcttca gaagctcaat gatagtataa cacgcaagat ccaagcaagg aacgagtacg acaaaaccat cttggagaca gaagcagcgt atacaaagat tttagaatcc gcgcaaacgc tgctgcacgt actcaaacgc gagtcggtgc atctgggcag aaaaacgtct ggcgaccggg cggaattgcc tggtattggc atcacgggag ttggtgtgga ctattcccca aatagcgtgt cggagctgtc gcatcgacgg gcggagaaag ggaaacccac agctggcgca ggataataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTSTTNSNL HNYNHELVAC IDDLRRKREE VHRQIIKVGA IPGPAWTRSF RLSKRMYHIL VFFISGLIRV KRTALGAVLL PQCTNARVAP WAVSVCHSSR KSTAARSLES AHLRQLNQCI FKDVTIFFTF LCMSQEEEDR AKVQKEIALL QERLQKLNDS ITRKIQARNE YDKTILETEA AYTKILESAQ TLLHVLKRES VHLGRKTSGD RAELPGIGIT GVGVDYSPNS VSELSHRRAE KGKPTAGAG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgagcaca acgaactcca acttacacaa ctacaaccac gagctcgttg cgtgcattga tgacctccgc cgcaagcgcg aagaggtcca ccgccagatc ataaaagtag gcgcaattcc tggtccagcg tggacaaggt catttcgact gtctaagcga atgtatcata ttcttgtttt ctttataagt ggtctcattc gtgtgaaacg aacagctctg ggagcggtct tgttgcctca gtgcacaaac gcccgcgtcg ctccttgggc tgtctctgtt tgtcattcat cccgtaagtc aactgcggca cgctctctgg agtctgccca tctcagacaa ctgaatcagt gcatcttcaa agatgtcacg atttttttca cgtttctgtg catgtctcag gaagaggagg atagagcgaa agtgcaaaag gagatcgcgc ttctgcagga acggcttcag aagctcaatg atagtataac acgcaagatc caagcaagga acgagtacga caaaaccatc ttggagacag aagcagcgta tacaaagatt ttagaatccg cgcaaacgct gctgcacgta ctcaaacgcg agtcggtgca tctgggcaga aaaacgtctg gcgaccgggc ggaattgcct ggtattggca tcacgggagt tggtgtggac tattccccaa atagcgtgtc ggagctgtcg catcgacggg cggagaaagg gaaacccaca gctggcgcag gaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa