TogoA.17181.a

unnamed apical complex protein

CENTER ID: TogoA.17181.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.17181.a.A2.GE29347 helical domain 834 1031
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_227000
OrthoMCL: OG5_240683
RefSeq: XP_002366353.1
UniProt: B6KET0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAPYSPRHV SKHPTLPARA VRFASQRFYR NYSPPPSSQT ETPADTPEPP SPLLAPERLF DFPGPATSLS RQFARAGQSL RAPVTGPTSK ERSISLSRAP HRSLSSLSLS SPRLSQDKYA TSHALEGRSV GRSDVVRGVV PVGMAGMSSF GVSGPGEKQH GSSPRAGSRG ATMSSFTSPA QSAPEAAVGS KEQQGVVDTP GNESAFAFSR AASRGFFVSD YENQQSSSDA FQCGDVPRTG AQHGPSEASG LYTPQDRLPQ EAQIRGSQLS SGRTETLQER QERLQREMDA LRQRQQLVTQ QARQLYQQKM ARPWLVPCNV SPDSTRLATL AIIPSKESRK GSCERSGSCP IFSRCARSGR RRSSGSRVRR IGVYVRPTAP SGQTVPPPAP GASFETNAAT VPGWFWENPG LLQCVAPVEV SCSDGKGGGV RSHPLVLKVD VVPSQTGSSL GKKEEDWKKS QASLVSSASA SETPKTDPRI VELAKENDTL RQDNALLKAQ LERAHAEGVR RRLTNIDLSR RSQVDVYGED GLLWGQEVEN EKLRRQIAEL GAALYAAAED GDFRLAAEAL TQQLTDAHQE IATLRKSMRS DSSGRQGPRC RDTSDDATLG ANDISVREAA VDSLISQGKR VTTLGRENVW KALSSMKDLV DQVFDRMTRL LTSTCTMKEK VQYLKGLHVL VVSSLDETLD AYEELAATCN KFDQIRAVVF DPKRNSPYCP CRPRRQVLED DLKHLCIQNK GATEKLRQLR ARIVKLEMEN LKFYVKHPDE PVRSSEARPV SPLAASLEVK ATETERLSSP KKDKKEKRDR RDISSKDDSA SATTAADDDS DRSGSVMRRA VAASRKKVWR KRQEVFPVRE DFSTYQLAST IKRLQDEIDR LKDDRQFDRT KAAEREQHLV SRVFNRNQIL VDKLAEQDRL LARLMQMIED RDCMHALAGP TSPTQLAQRQ ALHEDLEESR TLIRQSSVAL REGQTPEPQA FDNLITRLRT LKRDGLRDVT PEEQELLDMI NRQASQLRKI HQTVTELADH T
NT Sequence
atgagtgcac cgtactctcc caggcatgtg tccaaacatc caacgctccc tgcccgcgcc gttagattcg cgtctcaaag gttctaccgg aactactcgc ctccgccatc cagccagaca gagacccctg cggatacacc ggaacctcct tcgccgttgc tcgcccccga aagactcttt gacttccccg gtcctgccac cagcctctcc cgtcagttcg cgcgcgcggg acaaagtctc cgtgcgcccg ttacaggtcc tacttcaaag gaacgaagca tttcgctgtc gcgggcaccg caccgaagcc tttcttctct ttccctgtcc tctccaaggc tgtcccagga caaatacgcc accagccacg ccttggaagg gaggtccgtc ggccgctccg acgttgtccg cggggtggtt cctgtgggga tggctggaat gtcctccttt ggcgtgtctg gtcccggcga aaagcaacac gggtccagcc cgcgggcagg ttctcgcggc gccacgatga gcagcttcac gagtcccgcg cagtccgcac ccgaagcggc tgtcggatcg aaagaacagc aaggggtcgt cgacacccct ggaaacgaga gcgcctttgc cttctcccgt gctgcgtcgc gggggttttt cgtttcggac tatgaaaatc aacagagttc gtcggacgcc ttccaatgcg gtgacgtgcc taggaccggc gcgcagcatg ggccgagcga agcctctggc ctttatacac cccaggatcg attgccgcag gaagcgcaga ttcgaggtag ccagctttcc agtggtcgaa cggagactct ccaggagaga caagagcgac tgcagcggga aatggatgct ctgcggcagc ggcaacaact ggtcacgcag caggctcggc agctgtacca gcagaaaatg gctcgcccgt ggctggtccc ctgcaacgtt tctccggact ccacgcgcct cgccactctc gccatcattc cctcgaaaga gagcaggaaa ggcagctgcg agcgcagcgg cagctgtccg attttctcgc gatgcgccag aagtggccgc cgccgaagca gtggcagccg cgtccggcgc atcggggtgt acgtccgccc cactgcgccc agcggccaga cggtcccacc tccagctcca ggggcgagtt tcgagaccaa tgcagcaacc gtgccagggt ggttctggga aaaccccgga ttgctgcagt gtgtggcacc cgtggaggtt tcgtgttccg acggaaaggg cggaggcgtg cgttctcacc ccctcgttct gaaggtcgat gtcgtgccgt cgcagactgg gtcttctctg gggaagaagg aggaggattg gaagaagagt caggcaagcc ttgtcagttc tgcctcagcg tctgaaacgc cgaagacaga cccgcgaatc gtcgaactcg cgaaggaaaa cgacaccctc cgacaggaca acgctctgct caaggcacag ctggagcggg ctcatgccga gggtgtccgc cgacgtctta cgaacatcga tttgagtcgc cggtcccagg ttgatgtgta cggagaggat ggacttctct ggggacagga agtggaaaac gaaaagctcc gtcgtcaaat tgcggagctc ggagccgcgc tgtatgcggc tgcggaagat ggagatttcc gactggcggc cgaggccctg actcagcaac tgacggatgc ccaccaggaa attgccacat tgcgaaagtc aatgagatcc gactccagtg ggaggcaggg accgcggtgt cgagacacct cagacgacgc gacgctggga gcgaacgaca tttctgttcg tgaagccgct gtcgattctc tgatcagcca aggaaagcgc gtcacaactt tgggtcggga gaacgtctgg aaagctctga gttccatgaa ggatctagtg gaccaagttt tcgatcgcat gacacgactg ctgaccagca cctgcactat gaaggagaaa gtccagtacc tgaagggtct ccatgtgctc gttgttagca gtctcgacga aaccctcgat gcctacgagg aactcgccgc cacatgcaac aaattcgacc agattcgcgc ggtggttttc gatcctaaac gaaacagccc gtactgccca tgcagaccgc gacgtcaagt cctcgaagac gatctgaagc acctttgtat ccagaacaag ggtgcgacag agaagctgcg acagctccgg gcgcgcatag tcaaattgga aatggagaac ctaaagtttt atgtcaaaca tcccgatgag cctgtgagat cctcggaggc gcggccggtg tctccgctgg cggcgagcct ggaggtgaag gcgacggaga cagagcgtct ctcttctcca aaaaaagaca aaaaagaaaa aagagacaga agagacattt caagcaagga cgactcggcg agtgctacga ccgccgctga cgacgacagt gatcggagcg gatctgtgat gaggagagca gttgcggcaa gtcggaagaa ggtctggcga aagagacaag aagtcttccc cgtcagagag gacttttcta cgtaccagct cgcctctacg atcaaacggt tgcaggacga gattgatcgc ctgaaagacg atcgccagtt cgacagaacg aaggctgctg agcgggagca gcacctcgtc tctcgcgtct tcaacagaaa tcaaattctt gtggacaagt tggctgaaca agatcgcctc ttggcacggc tcatgcagat gattgaggac agagactgca tgcatgcact ggccggacca acgtcgccaa ctcaacttgc ccaacgtcag gcactccatg aggacctcga ggaaagcagg actctgatac gtcagtcttc ggtagcgctc cgagaaggac aaactccgga gcctcaggcg tttgacaatc tgatcactcg gcttagaact ctaaagcgcg atgggctgag agacgtgact ccagaggaac aggaactgct ggatatgatc aaccgtcagg cctctcagct acggaagatc caccagacgg tcactgagtt ggcggatcac acgtag
Details for TogoA.17181.a.A2.GE29347
HARVESTED ON: 5/3/2010
SEQUENCED ON: 3/4/2011
EXPECTED MW: 25kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGSVMRRAV AASRKKVWRK RQEVFPVRED FSTYQLASTI KRLQDEIDRL KDDRQFDRTK AAEREQHLVS RVFNRNQILV DKLAEQGRLL ARLMQMIEDR DCMHALAGPT SPTQLAQRQA LHEDLEESRT LIRQSSVALR EGQTPEPQAF DNLITRLRTL KRDGLRDVTP EEQELLDMIN RQASQLRKIH QTVTELA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggat ctgtgatgag gagagcagtt gcggcaagtc ggaagaaggt ctggcgaaag agacaagaag tcttccccgt cagagaggac ttttctacgt accagctcgc ctctacgatc aaacggttgc aggacgagat tgatcgcctg aaagacgatc gccagttcga cagaacgaag gctgctgagc gggagcagca cctcgtctct cgcgtcttca acagaaatca aattcttgtg gacaagttgg ctgaacaagg tcgcctcttg gcacggctca tgcagatgat tgaggacaga gactgcatgc atgcactggc cggaccaacg tcgccaactc aacttgccca acgtcaggca ctccatgagg acctcgagga aagcaggact ctgatacgtc agtcttcggt agcgctccga gaaggacaaa ctccggagcc tcaggcgttt gacaatctga tcactcggct tagaactcta aagcgcgatg ggctgagaga cgtgactcca gaggaacagg aactgctgga tatgatcaac cgtcaggcct ctcagctacg gaagatccac cagacggtca ctgagttggc ggat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGSVMRRAV AASRKKVWRK RQEVFPVRED FSTYQLASTI KRLQDEIDRL KDDRQFDRTK AAEREQHLVS RVFNRNQILV DKLAEQDRLL ARLMQMIEDR DCMHALAGPT SPTQLAQRQA LHEDLEESRT LIRQSSVALR EGQTPEPQAF DNLITRLRTL KRDGLRDVTP EEQELLDMIN RQASQLRKIH QTVTELADHT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggatctgtg atgaggagag cagttgcggc aagtcggaag aaggtctggc gaaagagaca agaagtcttc cccgtcagag aggacttttc tacgtaccag ctcgcctcta cgatcaaacg gttgcaggac gagattgatc gcctgaaaga cgatcgccag ttcgacagaa cgaaggctgc tgagcgggag cagcacctcg tctctcgcgt cttcaacaga aatcaaattc ttgtggacaa gttggctgaa caagatcgcc tcttggcacg gctcatgcag atgattgagg acagagactg catgcatgca ctggccggac caacgtcgcc aactcaactt gcccaacgtc aggcactcca tgaggacctc gaggaaagca ggactctgat acgtcagtct tcggtagcgc tccgagaagg acaaactccg gagcctcagg cgtttgacaa tctgatcact cggcttagaa ctctaaagcg cgatgggctg agagacgtga ctccagagga acaggaactg ctggatatga tcaaccgtca ggcctctcag ctacggaaga tccaccagac ggtcactgag ttggcggatc acacgaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa