TogoA.17172.a

unnamed apical complex protein

CENTER ID:	TogoA.17172.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.17172.a.A1.GE29339	full length		1	315

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_283510
OrthoMCL:	OG5_241441
RefSeq:	XP_002369110.1
UniProt:	B6KMN7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDCCGLCADD DRDVWMTDRI SPKIGLSDSD LLDFHSDDEM PVAPAAQCHR SQPSRQTSRV AASLPQKSER SPETRSQTSL NRLRSGGQFP PTRTSFDERQ GTTPLELQST SAKPPYRAPS LARAASKRRE PSPPARVATR SKAFDGDGSV PPPMSPAKSL SANDRVPTKL SELTPEEKEE EKKRLQALVK EFAKEAVAGF SIERIDNDTG VRAKTTFSMD RFLTTIDLRN DDDDSPLPHM SLPMEDLTGI FKGDDAVSHS SITGDCDLNR VLVLTSSDNP DVVLEAASRQ HRDKVYTCLK ILRLSFDIAR KDKRQ

NT Sequence

atggactgct gcggactgtg tgccgatgat gatcgggacg tgtggatgac cgaccgcatt tcacccaaga ttggcctctc cgacagcgac cttctcgatt tccatagcga tgatgaaatg cccgttgcgc cagcggctca gtgtcaccgg tcacagccca gccgccagac ctcacgggtg gctgcgtcgt tgccccagaa atcggaacgg tctccggaga cacgctccca gacttcgctt aacaggttgc gttccggagg tcagtttcca cccacacgca catcttttga cgaacgtcag ggcaccactc cacttgaact acagtcgacc tcggcaaagc cgccatacag ggccccgtca cttgcccgag cagcctccaa aaggcgagag ccctctcccc cagccagagt tgctacccgg tcaaaggcgt ttgacgggga tggcagcgtt ccaccgccaa tgtctccagc taagagtctt tccgcaaatg acagagtgcc cacgaaactg tctgaattga caccagaaga aaaggaggaa gagaagaaga gattgcaagc actcgttaaa gaatttgcga aggaagcagt agctggtttc tccattgagc gcattgacaa cgacacaggg gttcgagcga agacgacctt ctccatggac cgattcctca cgacgattga cttgcgtaac gacgacgacg acagcccgtt gccccacatg tcacttccca tggaggatct gactggtatt tttaagggtg acgacgcggt ctcacattca tctatcaccg gtgactgcga cctcaaccgt gttcttgtct taacatcgtc agacaatcct gatgttgttc ttgaagcagc gtcacgccaa catcgggaca aggtgtacac ttgcttgaag atcctcaggt tgtcatttga tattgccaga aaagataagc ggcagtga

Details for TogoA.17172.a.A1.GE29339

HARVESTED ON:	5/3/2010
SEQUENCED ON:	2/9/2011
EXPECTED MW:	37kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMDCCGLC ADDDRDVWMT DRISPKIGLS DSDLLDFHSD DEMPVAPAAQ CHRSQPSRQT SRVAASLPQK SERSPETRSQ TSLNRLRSGG QFPPTRTSFD ERQGTTPLEL QSTSAKPPYR APSLARAASK RREPSPPARV ATRSKAFDGD GSVPPPMSPA KSLSANDRVP TKLSELTPEE KEEEKKRLQA LVKEFAKEAV AGFSIERIDN DTGVRAKTTF SMDRFLTTID LRNDDDDSPL PHMSLPMEDL TGIFKGDDAV SHSSITGDCD LNRVLVLTSS DNPDVVLEAA SRQHRDKVYT CLKILRLSFD IARKDKRQ

Validated NT Sequence

ttattactgc cgcttatctt ttctggcaat atcaaatgac aacctgagga tcttcaagca agtgtacacc ttgtcccgat gttggcgtga cgctgcttca agaacaacat caggattgtc tgacgatgtt aagacaagaa cacggttgag gtcgcagtca ccggtgatag atgaatgtga gaccgcgtcg tcacccttaa aaataccagt cagatcctcc atgggaagtg acatgtgggg caacgggctg tcgtcgtcgt cgttacgcaa gtcaatcgtc gtgaggaatc ggtccatgga gaaggtcgtc ttcgctcgaa cccctgtgtc gttgtcaatg cgctcaatgg agaaaccagc tactgcttcc ttcgcaaatt ctttaacgag tgcttgcaat ctcttcttct cttcctcctt ttcttctggt gtcaattcag acagtttcgt gggcactctg tcatttgcgg aaagactctt agctggagac attggcggtg gaacgctgcc atccccgtca aacgcctttg accgggtagc aactctggct gggggagagg gctctcgcct tttggaggct gctcgggcaa gtgacggggc cctgtatggc ggctttgccg aggtcgactg tagttcaagt ggagtggtgc cctgacgttc gtcaaaagat gtgcgtgtgg gtggaaactg acctccggaa cgcaacctgt taagcgaagt ctgggagcgt gtctccggag accgttccga tttctggggc aacgacgcag ccacccgtga ggtctggcgg ctgggctgtg accggtgaca ctgagccgct ggcgcaacgg gcatttcatc atcgctatgg aaatcgagaa ggtcgctgtc ggagaggcca atcttgggtg aaatgcggtc ggtcatccac acgtcccgat catcatcggc acacagtccg cagcagtcca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag c

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDCCGLCAD DDRDVWMTDR ISPKIGLSDS DLLDFHSDDE MPVAPAAQCH RSQPSRQTSR VAASLPQKSE RSPETRSQTS LNRLRSGGQF PPTRTSFDER QGTTPLELQS TSAKPPYRAP SLARAASKRR EPSPPARVAT RSKAFDGDGS VPPPMSPAKS LSANDRVPTK LSELTPEEKE EEKKRLQALV KEFAKEAVAG FSIERIDNDT GVRAKTTFSM DRFLTTIDLR NDDDDSPLPH MSLPMEDLTG IFKGDDAVSH SSITGDCDLN RVLVLTSSDN PDVVLEAASR QHRDKVYTCL KILRLSFDIA RKDKRQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggactgctgc ggactgtgtg ccgatgatga tcgggacgtg tggatgaccg accgcatttc acccaagatt ggcctctccg acagcgacct tctcgatttc catagcgatg atgaaatgcc cgttgcgcca gcggctcagt gtcaccggtc acagcccagc cgccagacct cacgggtggc tgcgtcgttg ccccagaaat cggaacggtc tccggagaca cgctcccaga cttcgcttaa caggttgcgt tccggaggtc agtttccacc cacacgcaca tcttttgacg aacgtcaggg caccactcca cttgaactac agtcgacctc ggcaaagccg ccatacaggg ccccgtcact tgcccgagca gcctccaaaa ggcgagagcc ctctccccca gccagagttg ctacccggtc aaaggcgttt gacggggatg gcagcgttcc accgccaatg tctccagcta agagtctttc cgcaaatgac agagtgccca cgaaactgtc tgaattgaca ccagaagaaa aggaggaaga gaagaagaga ttgcaagcac tcgttaaaga atttgcgaag gaagcagtag ctggtttctc cattgagcgc attgacaacg acacaggggt tcgagcgaag acgaccttct ccatggaccg attcctcacg acgattgact tgcgtaacga cgacgacgac agcccgttgc cccacatgtc acttcccatg gaggatctga ctggtatttt taagggtgac gacgcggtct cacattcatc tatcaccggt gactgcgacc tcaaccgtgt tcttgtctta acatcgtcag acaatcctga tgttgttctt gaagcagcgt cacgccaaca tcgggacaag gtgtacactt gcttgaagat cctcaggttg tcatttgata ttgccagaaa agataagcgg cagaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa