TogoA.17162.a

Hsp20/alpha crystallin domain-containing protein

CENTER ID:	TogoA.17162.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.17162.a.A1.GE29328	full length		1	272

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_289600
OrthoMCL:	OG5_126935
RefSeq:	XP_002368385.1
UniProt:	B9PTP1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADSSGPGDA RVAQRVTTIP GGTTTTTKTT TYTIRSVPVT TTTSSFAASS CGTTTGPTTS ATDFAFDDLK RYLEDHGKMI LEDPRTKHLI ASGQATWEDV KRYLDSRAPP THLAEGSSVV YSTTGGYWYP VVAGMPGVAA PSTIFEVGPG SAEICNKISF RPRLDAYYDA NRNRLVLLFD LPGFEKKDVE IELDKGALAI SGERPKLEES KLGQDCNNII KERSFGFFYR KFQLPGNAEE ESIKASMEQG VLEVTIGLKD ENQPTKKKID VQ

NT Sequence

atggcagact caagcgggcc gggggacgcg cgtgtcgctc agcgcgttac tacgattcct ggcggaacaa caacaacaac aaaaacgact acttacacca tccgtagcgt cccagtaaca acgactacca gctcttttgc ggccagttcg tgcggaacca ccaccggacc cacaacatca gcgacagact tcgccttcga cgatttaaaa cgctacttgg aagatcacgg caagatgata ttggaagatc ctcgaaccaa acacctgatt gcatcaggac aggctacctg ggaagacgtg aagcggtact tggattcacg tgcgccacca acacacctcg ctgaagggag tagcgtagtc tacagcacaa caggtggcta ctggtatcct gtcgtcgctg gaatgcccgg ggttgccgca cccagcacaa ttttcgaagt gggcccaggt tcagcggaaa tttgcaacaa gatctctttc cgacctcgcc ttgacgccta ctacgacgcc aatcgaaatc gattagttct tctgttcgac cttcccggtt tcgagaagaa agacgtcgag atcgagttgg acaaaggtgc tcttgcaata tccggcgagc gacctaagct ggaggaaagc aagttaggtc aggattgcaa taatataatc aaagaacgat catttggatt tttctacagg aagtttcagc tgcctggaaa cgcggaagag gagagtatca aagcctcgat ggagcaagga gttttggaag taacgattgg cctgaaagac gaaaaccaac caacgaagaa aaagattgat gtgcagtga

Details for TogoA.17162.a.A1.GE29328

HARVESTED ON:	5/3/2010
SEQUENCED ON:	5/6/2010
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	93
PERCENT COVERAGE:	83

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMADSSGPGD ARVAQRVTTI PGGTTTTTKT TTYTIRSVPV TTTTSSFAAS SCGTTTGPTT SATDFAFDDL KRYLEDHGKM ILEDPRTKHL IASGQATWED VKRYLDSRAP PTHLAEGSSV VYSTTGGYWY PVVAGMPGVA APSTIFEVGP GSAEICNKIS FRPRLDAYYD ANRNRLVLLF DLPGFEKKDV EIELDKGALA ISGSFSCLET RKRRVSKPRW SKEFWK

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcag actcaagcgg gccgggggac gcgcgtgtcg ctcagcgcgt tactacgatt cctggcggaa caacaacaac aacaaaaacg actacttaca ccatccgtag cgtcccagta acaacgacta ccagctcttt tgcggccagt tcgtgcggaa ccaccaccgg acccacaaca tcagcgacag acttcgcctt cgacgattta aaacgctact tggaagatca cggcaagatg atattggaag atcctcgaac caaacacctg attgcatcag gacaggctac ctgggaagac gtgaagcggt acttggattc acgtgcgcca ccaacacacc tcgctgaagg gagtagcgta gtctacagca caacaggtgg ctactggtat cctgtcgtcg ctggaatgcc cggggttgcc gcacccagca caattttcga agtgggccca ggttcagcgg aaatttgcaa caagatctct ttccgacctc gccttgacgc ctactacgac gccaatcgaa atcgattagt tcttctgttc gaccttcccg gtttcgagaa gaaagacgtc gagatcgagt tggacaaagg tgctcttgca atatccggaa gtttcagctg cctggaaacg cggaagagga gagtatcaaa gcctcgatgg agcaaggagt tttggaagta acgattggcc tgaaagacga aaaccaacca acgaagaaaa agattgatgt gcagtaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagactca agcgggccgg gggacgcgcg tgtcgctcag cgcgttacta cgattcctgg cggaacaaca acaacaacaa aaacgactac ttacaccatc cgtagcgtcc cagtaacaac gactaccagc tcttttgcgg ccagttcgtg cggaaccacc accggaccca caacatcagc gacagacttc gccttcgacg atttaaaacg ctacttggaa gatcacggca agatgatatt ggaagatcct cgaaccaaac acctgattgc atcaggacag gctacctggg aagacgtgaa gcggtacttg gattcacgtg cgccaccaac acacctcgct gaagggagta gcgtagtcta cagcacaaca ggtggctact ggtatcctgt cgtcgctgga atgcccgggg ttgccgcacc cagcacaatt ttcgaagtgg gcccaggttc agcggaaatt tgcaacaaga tctctttccg acctcgcctt gacgcctact acgacgccaa tcgaaatcga ttagttcttc tgttcgacct tcccggtttc gagaagaaag acgtcgagat cgagttggac aaaggtgctc ttgcaatatc cggcgagcga cctaagctgg aggaaagcaa gttaggtcag gattgcaata atataatcaa agaacgatca tttggatttt tctacaggaa gtttcagctg cctggaaacg cggaagagga gagtatcaaa gcctcgatgg agcaaggagt tttggaagta acgattggcc tgaaagacga aaaccaacca acgaagaaaa agattgatgt gcagaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa