TogoA.17159.a

thioredoxin-like, protein

CENTER ID: TogoA.17159.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.17159.a.A1.GE29325 full length 1 220
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_232410
RefSeq: XP_002368111.1
UniProt: Q8MPF4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQPVFASPL NVEKRRLNEE RALMQAQKAG GEGVNIQLPP NYGDMDLILF PEGSLKNSNN TVIPQSHLKG KSVALYFADG ADPKCASLLP FLLNYYRTMN EGGANQKIEI IFVSLDRDRE AFESHRAHMP WLSIDLENPL TEILKRHFRV MKEYEVPTYG YGSRTGVPSV IVIGSDGREA QFLPICSGLE EGDRALLRWD WRNTKFASDQ FHVRPTLLEQ
NT Sequence
atgtcgcaac ctgtgttcgc gtctccactg aacgtggaaa agaggaggct taatgaagag cgggctttga tgcaggccca gaaggcaggc ggtgaaggtg tgaacattca attgccaccc aactacggag atatggacct catccttttc ccggagggca gcctgaagaa ttcgaacaac acggtcattc ctcagtccca cttgaaggga aaatccgtgg ctctttactt cgctgatggt gccgacccga agtgcgcgtc tctcctcccc tttctgctca actattaccg gacgatgaat gagggcggcg ccaaccagaa gatagagatc atttttgtta gtttggatcg agatcgcgag gcttttgaga gtcatcgagc gcacatgccg tggctcagca tcgacttgga aaatcctttg acagagatct taaagcgtca ttttcgagtc atgaaggaat atgaggtgcc tacgtatgga tacggcagca ggactggcgt cccgagtgtc atcgtgattg gcagtgacgg gcgtgaagcc cagttcctcc ctatctgcag cggcctcgag gaaggcgaca gagctcttct tcgctgggac tggagaaaca cgaagttcgc ctctgaccag ttccacgttc gcccaaccct tttagagcag tag
Details for TogoA.17159.a.A1.GE29325
HARVESTED ON: 5/3/2010
SEQUENCED ON: 3/4/2011
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSQPVFASP LNVEKRRLNE ERALMQAQKA GGEGVNIQLP PNYGDMDLIL FPEGSLKNSN NTVIPQSHLK GKSVALYFAD GADPKCASLL PFLLNYYRTM NEGGANQKIE IIFVSLDRDR EAFESHRAHM PWLSIDLENP LTEILKRHFR VMKEYEVPTY GYGSRTGVPS VIVIGSDGRE AQFLPICSGL EEGDRALLRW DWRNTKFASD QFHVRPTLL
Validated NT Sequence
ctaaaagggt tgggcgaacg tggaactggt cagaggcgaa cttcgtgttt ctccagtccc agcgaagaag agctctgtcg ccttcctcga ggccgctgca gatagggagg aactgggctt cacgcccgtc actgccaatc acgatgacac tcgggacgcc agtcctgctg ccgtatccat acgtaggcac ctcatattcc ttcatgactc gaaaatgacg ctttaagatc tctgtcaaag gattttccaa gtcgatgctg agccacggca tgtgcgctcg atgactctca aaagcctcgc gatctcgatc caaactaaca aaaatgatct ctatcttctg gttggcgccg ccctcattca tcgtccggta atagttgagc agaaagggga ggagagacgc gcacttcggg tcggcaccat cagcgaagta aagagccacg gattttccct tcaagtggga ctgaggaatg accgtgttgt tcgaattctt caggctgccc tccgggaaaa ggatgaggtc catatctccg tagttgggtg gcaattgaat gttcacacct tcaccgcctg ccttctgggc ctgcatcaaa gcccgctctt cattaagcct cctcttttcc acgttcagtg gagacgcgaa cacaggttgc gacatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQPVFASP LNVEKRRLNE ERALMQAQKA GGEGVNIQLP PNYGDMDLIL FPEGSLKNSN NTVIPQSHLK GKSVALYFAD GADPKCASLL PFLLNYYRTM NEGGANQKIE IIFVSLDRDR EAFESHRAHM PWLSIDLENP LTEILKRHFR VMKEYEVPTY GYGSRTGVPS VIVIGSDGRE AQFLPICSGL EEGDRALLRW DWRNTKFASD QFHVRPTLLE Q
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgcaacct gtgttcgcgt ctccactgaa cgtggaaaag aggaggctta atgaagagcg ggctttgatg caggcccaga aggcaggcgg tgaaggtgtg aacattcaat tgccacccaa ctacggagat atggacctca tccttttccc ggagggcagc ctgaagaatt cgaacaacac ggtcattcct cagtcccact tgaagggaaa atccgtggct ctttacttcg ctgatggtgc cgacccgaag tgcgcgtctc tcctcccctt tctgctcaac tattaccgga cgatgaatga gggcggcgcc aaccagaaga tagagatcat ttttgttagt ttggatcgag atcgcgaggc ttttgagagt catcgagcgc acatgccgtg gctcagcatc gacttggaaa atcctttgac agagatctta aagcgtcatt ttcgagtcat gaaggaatat gaggtgccta cgtatggata cggcagcagg actggcgtcc cgagtgtcat cgtgattggc agtgacgggc gtgaagccca gttcctccct atctgcagcg gcctcgagga aggcgacaga gctcttcttc gctgggactg gagaaacacg aagttcgcct ctgaccagtt ccacgttcgc ccaacccttt tagagcagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa