TogoA.17142.a

Membrane occupation and recognition nexus protein (TgMORN1)

CENTER ID: TogoA.17142.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.17142.a.A1.GE29308 full length 1 363
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_310440
OrthoMCL: OG5_128972
RefSeq: XP_002364290.1
UniProt: Q3S2E8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MESCHAYHGQ IKDGLFHGKG TLIYSGNEKY EGEFVFGKRE GHGRFLYADG ATYEGKWVED RIHGQGVAHF ASGNRYEGQW EMGRINGFGK LSYSNGDEYE GEWVDGKMHG RGTYRYAEGD VYTGEWRDDK RHGKGSVTYV SAKGSVVEKY EGDWVNGKMH GHGKYIYSDG GVYEGDWIDG KMHGKGTYVF PNGNVYEGEW AHDMKDGYGV LTYQNGEKYE GYWKQDKVHG KGTLTYTRGD KYIGDWMDAK KDGEGELIYA NGDRFKGQWA DDRANGFGVF TYANGNRYEG EWTDDKRHGR GVFYCAEDGS AYEGEFVGGR KEGNGILRLA TGHQLEGTWS GGQLVRVTSF VFAQDSPWLN VDL
NT Sequence
atggagagct gccacgcgta ccacggacag atcaaagacg gtctgtttca cgggaaaggg actctgattt acagcggaaa tgaaaagtac gagggggagt tcgtgttcgg gaagcgcgag gggcacggtc gcttcctcta cgccgacgga gccacttacg aagggaagtg ggtcgaagac cgcatccacg gccagggcgt cgcacatttc gccagtggca accggtacga aggccagtgg gaaatgggcc gaatcaacgg cttcgggaag ctttcgtaca gcaacggtga cgagtacgag ggcgagtggg tggacgggaa gatgcacggt cgcgggacct accgctacgc ggagggcgat gtctatacag gggagtggcg cgacgataag cgccacggaa aaggcagtgt gacgtacgtc agtgccaaag gttccgtggt ggagaaatac gagggcgact gggtgaacgg aaagatgcac ggtcacggga agtacatcta cagcgacggc ggcgtctacg agggagactg gatcgacggg aagatgcatg gaaagggcac ttatgtgttt ccgaacggca acgtgtacga aggcgagtgg gctcacgaca tgaaggacgg ctacggggtc ctgacgtacc agaacggaga aaagtacgaa gggtactgga agcaggacaa ggtccacggc aaaggcacac tcacttacac ccgcggtgac aagtacatcg gcgactggat ggacgcaaag aaggacgggg aaggcgagct catctacgcg aacggcgacc gcttcaaagg ccagtgggcc gacgaccgag caaacggatt cggcgtcttc acgtatgcaa acgggaaccg atacgaaggc gaatggacag acgacaaacg ccacggacgc ggagtcttct actgcgctga agacggcagc gcgtacgagg gcgagttcgt cggagggaga aaagaaggca atggaattct gcgtctcgcg actggccacc aactcgaagg cacctggtct ggtggacagc tcgtgcgcgt cacgtccttc gtcttcgccc aggactcacc atggctcaat gtcgacttgt aa
Details for TogoA.17142.a.A1.GE29308
HARVESTED ON: 5/3/2010
SEQUENCED ON: 2/9/2011
EXPECTED MW: 43kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 72
Validated AA Sequence
MGRINGFGKL SYSNGDEYEG EWVDGKMHGR GTYRYAEGDV YTGEWRDDKR HGKGSVTYVS AKGSVVEKYE GDWVNGKMHG HGKYIYSDGG VYEGDWIDGK MHGKGTYVFP NGNVYEGEWA HDMKDGYGVL TYQNGEKYEG YWKQDKVHGK GTLTYTRGDK YIGDWMDAKK DGEGELIYAN GDRFKGQWAD DRANGFGVFT YANGNRYEGE WTDDKRHGRG VFYCAEDGSA YEGEFVGGRK EGNGILRLAT GHQLEGTWSG GQLVRVTSFV FAQDSPWLNV
Validated NT Sequence
anacngnctg tttcnnngga aaggnnntnn gatttacagc ggaaatgaaa agtacgaggg ggagttcgtg ttcgggaagc gcgaggggca cggtcgcttc ctctacgccg acggagccac ttacgaaggg aagtgggtcg aagaccgcat ccacggccag ggcgtcgcac atttcgccag tggcaaccgg tacgaaggcc agtgggaaat gggccgaatc aacggcttcg ggaagctttc gtacagcaac ggtgacgagt acgagggcga gtgggtggac gggaagatgc acggtcgcgg gacctaccgc tacgcggagg gcgatgtcta tacaggggag tggcgcgacg ataagcgcca cggaaaaggc agtgtgacgt acgtcagtgc caaaggttcc gtggtggaga aatacgaggg cgactgggtg aacggaaaga tgcacggtca cgggaagtac atctacagcg acggcggcgt ctacgaggga gactggatcg acgggaagat gcatggaaag ggcacttatg tgtttccgaa cggcaacgtg tacgaaggcg agtgggctca cgacatgaag gacggctacg gggtcctgac gtaccagaac ggagaaaagt acgaagggta ctggaagcag gacaaggtcc acggcaaagg cacactcact tacacccgcg gtgacaagta catcggcgac tggatggacg caaagaagga cggggaaggc gagctcatct acgcgaacgg cgaccgcttc aaaggccagt gggccgacga ccgagcaaac ggattcggcg tcttcacgta tgcaaacggg aaccgatacg aaggcgaatg gacagacgac aaacgccacg gacgcggagt cttctactgc gctgaagacg gcagcgcgta cgagggcgag ttcgtcggag ggagaaaaga aggcaatgga attctgcgtc tcgcgactgg ccaccaactc gaaggcacct ggtctggtgg acagctcgtg cgcgtcacgt ccttcgtctt cgcccaggac tcaccatggc tcaatgtcg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMESCHAYHG QIKDGLFHGK GTLIYSGNEK YEGEFVFGKR EGHGRFLYAD GATYEGKWVE DRIHGQGVAH FASGNRYEGQ WEMGRINGFG KLSYSNGDEY EGEWVDGKMH GRGTYRYAEG DVYTGEWRDD KRHGKGSVTY VSAKGSVVEK YEGDWVNGKM HGHGKYIYSD GGVYEGDWID GKMHGKGTYV FPNGNVYEGE WAHDMKDGYG VLTYQNGEKY EGYWKQDKVH GKGTLTYTRG DKYIGDWMDA KKDGEGELIY ANGDRFKGQW ADDRANGFGV FTYANGNRYE GEWTDDKRHG RGVFYCAEDG SAYEGEFVGG RKEGNGILRL ATGHQLEGTW SGGQLVRVTS FVFAQDSPWL NVDL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagagctgc cacgcgtacc acggacagat caaagacggt ctgtttcacg ggaaagggac tctgatttac agcggaaatg aaaagtacga gggggagttc gtgttcggga agcgcgaggg gcacggtcgc ttcctctacg ccgacggagc cacttacgaa gggaagtggg tcgaagaccg catccacggc cagggcgtcg cacatttcgc cagtggcaac cggtacgaag gccagtggga aatgggccga atcaacggct tcgggaagct ttcgtacagc aacggtgacg agtacgaggg cgagtgggtg gacgggaaga tgcacggtcg cgggacctac cgctacgcgg agggcgatgt ctatacaggg gagtggcgcg acgataagcg ccacggaaaa ggcagtgtga cgtacgtcag tgccaaaggt tccgtggtgg agaaatacga gggcgactgg gtgaacggaa agatgcacgg tcacgggaag tacatctaca gcgacggcgg cgtctacgag ggagactgga tcgacgggaa gatgcatgga aagggcactt atgtgtttcc gaacggcaac gtgtacgaag gcgagtgggc tcacgacatg aaggacggct acggggtcct gacgtaccag aacggagaaa agtacgaagg gtactggaag caggacaagg tccacggcaa aggcacactc acttacaccc gcggtgacaa gtacatcggc gactggatgg acgcaaagaa ggacggggaa ggcgagctca tctacgcgaa cggcgaccgc ttcaaaggcc agtgggccga cgaccgagca aacggattcg gcgtcttcac gtatgcaaac gggaaccgat acgaaggcga atggacagac gacaaacgcc acggacgcgg agtcttctac tgcgctgaag acggcagcgc gtacgagggc gagttcgtcg gagggagaaa agaaggcaat ggaattctgc gtctcgcgac tggccaccaa ctcgaaggca cctggtctgg tggacagctc gtgcgcgtca cgtccttcgt cttcgcccag gactcaccat ggctcaatgt cgacttgaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa