TogoA.17128.a

Putative uncharacterized protein (GAP45)

CENTER ID:	TogoA.17128.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.17128.a.A1.GE28256	full length		1	245

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_223940
OrthoMCL:	OG5_147428
RefSeq:	XP_002366080.1
UniProt:	B6KE07

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGNACKKNTA KTPTRKEAED LAEKERQERE AKEKAEAEEK ARAEAEKNAA DKAEAERRAA EAREREESAR KEAEAEAARK AEAEAAEAER LRKEAEKKKA EEAKRKAEEE QRAAAEEAEQ RAREEAERRK AEAAAAAERE RQMQEALKQE EMSPREKYDK LASPEDSASE TTMATQPQKV AEHSSAAVTD RSVVGYTVTP CDMASIDETA KYLSKRCGCD LGDQHDENEC PICRHIDLSD APLLN

NT Sequence

atgggaaacg cgtgcaagaa gaacacggcc aagacgccga cccggaagga ggcggaggac ctggctgaga aggagcggca ggagcgggag gcgaaggaga aggctgaggc tgaagagaag gctcgcgccg aagcggagaa gaacgcagca gacaaagcgg aggctgaacg tagagcggca gaggcgcgag agcgcgaaga atcagccagg aaggaggcgg aggctgaggc ggcccgcaag gccgaagcag aggcggctga ggccgagcgc ctccggaagg aagcagagaa gaaaaaggcg gaggaggcga aacgcaaggc ggaggaggag cagcgcgccg cagccgagga agcggagcaa agagcgcggg aggaggccga gcgccgcaaa gctgaagctg cggcggcagc ggagcgcgag cgccagatgc aggaggcgct gaagcaagag gaaatgtcac cgagagagaa gtacgacaag ttagccagcc ccgaagactc cgcatccgag accacgatgg cgacacagcc gcagaaagtc gccgagcaca gcagcgcggc ggtcacagac agatcagtgg tggggtacac cgtgactcca tgcgacatgg catcaattga cgagacagct aagtacttgt caaagcgctg cggttgcgac ctaggcgacc aacacgacga aaacgagtgc cctatttgcc gccacatcga cttgtcggat gcacccttgt tgaactga

Details for TogoA.17128.a.A1.GE28256

HARVESTED ON:	data unavailable
SEQUENCED ON:	2/22/2010
EXPECTED MW:	29kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMGNACKK NTAKTPTRKE AEDLAEKERQ EREAKEKAEA EEKARAEAEK NAADKAEAER RAAEAREREE SARKEAEAEA ARKAEAEAAE AERLRKEAEK KKAEEAKRKA EEEQRAAAEE AEQRAREEAE RRKAEAAAAA ERERQMQEAL KQEEMSPREK YDKLASPEDS ASETTMATQP QKVAEHSSAA VTDRSVVGYT VTPCDMASID ETAKYLSKRC GCDLGDQHDE NECPICRHID LSDAPLLN

Validated NT Sequence

tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggaaacgcg tgcaagaaga acacggccaa gacgccgacc cggaaggagg cggaggacct ggctgagaag gagcggcagg agcgggaggc gaaggagaag gctgaggctg aagagaaggc tcgcgccgaa gcggagaaga acgcagcaga caaagcggag gctgaacgta gagcggcaga ggcgcgagag cgcgaagaat cagccaggaa ggaggcggag gctgaggcgg cccgcaaggc cgaagcagag gcggctgagg ccgagcgcct ccggaaggaa gcagagaaga aaaaggcgga ggaggcgaaa cgcaaggcgg aggaggagca gcgcgccgca gccgaggaag cggagcaaag agcgcgggag gaggccgagc gccgcaaagc tgaagctgcg gcggcagcgg agcgcgagcg ccagatgcag gaggcgctga agcaagagga aatgtcaccg agagagaagt acgacaagtt agccagcccc gaagactccg catccgagac cacgatggcg acacagccgc agaaagtcgc cgagcacagc agcgcggcgg tcacagacag atcagtggtg gggtacaccg tgactccatg cgacatggca tcaattgacg agacagctaa gtacttgtca aagcgctgcg gttgcgacct aggcgaccaa cacgacgaaa acgagtgccc tatttgccgc cacatcgact tgtcggatgc acccttgttg aactaataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGNACKKNT AKTPTRKEAE DLAEKERQER EAKEKAEAEE KARAEAEKNA ADKAEAERRA AEAREREESA RKEAEAEAAR KAEAEAAEAE RLRKEAEKKK AEEAKRKAEE EQRAAAEEAE QRAREEAERR KAEAAAAAER ERQMQEALKQ EEMSPREKYD KLASPEDSAS ETTMATQPQK VAEHSSAAVT DRSVVGYTVT PCDMASIDET AKYLSKRCGC DLGDQHDENE CPICRHIDLS DAPLLN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggaaacgcg tgcaagaaga acacggccaa gacgccgacc cggaaggagg cggaggacct ggctgagaag gagcggcagg agcgggaggc gaaggagaag gctgaggctg aagagaaggc tcgcgccgaa gcggagaaga acgcagcaga caaagcggag gctgaacgta gagcggcaga ggcgcgagag cgcgaagaat cagccaggaa ggaggcggag gctgaggcgg cccgcaaggc cgaagcagag gcggctgagg ccgagcgcct ccggaaggaa gcagagaaga aaaaggcgga ggaggcgaaa cgcaaggcgg aggaggagca gcgcgccgca gccgaggaag cggagcaaag agcgcgggag gaggccgagc gccgcaaagc tgaagctgcg gcggcagcgg agcgcgagcg ccagatgcag gaggcgctga agcaagagga aatgtcaccg agagagaagt acgacaagtt agccagcccc gaagactccg catccgagac cacgatggcg acacagccgc agaaagtcgc cgagcacagc agcgcggcgg tcacagacag atcagtggtg gggtacaccg tgactccatg cgacatggca tcaattgacg agacagctaa gtacttgtca aagcgctgcg gttgcgacct aggcgaccaa cacgacgaaa acgagtgccc tatttgccgc cacatcgact tgtcggatgc acccttgttg aacaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa