TogoA.17127.a
Glucose-6-phosphate isomerase (EC 5.3.1.9)
| CENTER ID: | TogoA.17127.a |
| ORGANISM: | Toxoplasma gondii ME49 |
| ASSOCIATED DISEASE: | Toxoplasmosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TogoA.17127.a.A1.GE28255 | full length | 1 | 563 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TogoA.17127.a.A1.PW28176 | full length | 1 | 563 |
Structures
3UJH
DEPOSITED: 11/7/2011
DETERMINATION: XRay
CLONE: TogoA.17127.a.A1.GE28255
PROTEIN: TogoA.17127.a.A1.PW28176
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | ToxoDB:TGME49_283780 |
| OrthoMCL: | OG5_126980 |
| RefSeq: | XP_002369127.1 |
| UniProt: | B6KMQ4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAPTQLEQCA SHGKLLQEKK KLEKLHLRDL LKDEARNDLL IRSTDQGVYL DFSRQKITLE TLQHLVNLAH ERQVPAMVKR MFSGEKINQT ENRAVLHVAL RMPEGSEPVH VDGKNVLDEV HAVLRRIRVF SEKVRSGEIR GHTGKKLVNV ISIGIGGSYL GTEFVHLALA AEGYAAEKAH GRQIHFLANV DPVDVWLAER GFDPEETLVV VISKTFTTAE TMMNARSVRD WYLHHYKGDE RALGAHFCAV STNLDGTSKF GIQSDRVFGF WDWVGGRYSV TSAVGILPLA LQYGYDVAQE FLNGAHAMDV HFKTAELADN LPMLMGLISV WNATFFGYSN VAVLPYAQAL LRFPAHIQQL TMESNGKRVT MDGKTLDFDV GEIFFGEPGT NGQHSFYQLI HQGRVIPAEF IGFCKSQRAI KLKEEPVSNH DELMSNFFAQ PDALAFGKTP EELRKEGIPE KLVPHKTFPG DRPSCMLLFP EISPFHIGQL LALYEHRVAV EGWLWGINSF DQWGVELGKV LAKGVRGILQ KRREGKAPHE SGQSELCSST RKILEHYVQQ SKA
NT Sequence
atggcgccga cacagctgga acagtgcgcc agtcacggca agctgctgca ggagaagaaa aagctcgaga agcttcattt gcgggacttg ctcaaggacg aagcgcgaaa tgacctcctc atcagatcca ctgaccaggg cgtctacctg gactttagcc gccagaaaat aacgctggaa acccttcagc atcttgtgaa tcttgcacac gagcgacaag tgccagcgat ggtgaagcgc atgttttcgg gagagaagat caaccagacc gagaaccgcg ccgtgttgca tgtcgccttg cgcatgcccg aaggcagcga gccggtccac gtcgatggca aaaatgttct ggatgaagtg catgcagtgc tgagacgcat tcgcgtcttt tccgagaaag tccgcagtgg tgaaattcga ggccacactg ggaagaaact cgtgaacgtc atttcaattg gcattggcgg aagctacttg gggactgagt ttgttcatct cgctctcgca gccgaaggct atgccgctga gaaggcacac ggccgtcaga ttcacttttt agcgaacgtg gacccggtcg atgtgtggct tgccgagaga ggcttcgacc ctgaggagac tctcgtggtt gtcattagca agacgttcac gacagcagag acgatgatga atgctcgctc tgttcgggac tggtacctcc accactacaa gggagatgaa cgcgcgttgg gtgcgcactt ctgcgcggtt agcacgaacc tggatggcac gagcaagttc ggcattcagt cggaccgtgt cttcggcttc tgggactggg tgggcgggag gtactccgtc acctcggctg tgggcattct tcctctcgct ctccaatacg gctatgacgt cgcgcaagag tttctgaatg gcgcgcatgc catggatgtt cacttcaaga cggccgagct ggcagacaat cttccgatgc tcatgggtct catcagtgtg tggaacgcca ctttcttcgg ctactcgaat gtcgccgttc tgccctacgc gcaggctctt ctccgcttcc ctgcacacat ccaacagctt accatggaga gcaacgggaa gcgcgtgacg atggatggca agaccctgga tttcgatgtg ggcgaaattt tctttggaga gccgggcacc aacggccagc acagtttcta ccagctcatc caccaaggtc gtgtcattcc tgcggagttc attggctttt gcaagtctca gagagcgatc aagctgaagg aggagcccgt aagcaaccac gacgaactga tgagcaactt ctttgctcag ccagatgcgc tggctttcgg aaagacaccc gaagaactcc gcaaggaagg cattccggag aagctcgttc cccacaagac cttccctggc gaccgtcctt cgtgcatgct tctttttccg gagatctcac ccttccatat tggccagctg ctggcgttgt acgagcaccg cgttgctgtg gagggttggc tgtggggaat caactcgttc gatcagtggg gtgttgaact tggaaaggtc ctcgccaagg gcgtccgtgg catcctccag aagcgtcgtg agggcaaggc accccatgag tctggacaaa gcgaactctg cagctcgacg aggaagattt tggagcacta cgtgcagcag tcgaaggctt aa
Details for TogoA.17127.a.A1.GE28255
| HARVESTED ON: | 2/2/2010 |
| SEQUENCED ON: | 2/22/2010 |
| EXPECTED MW: | 65kDa |
| OBSERVED MW: | 65kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAPTQLEQC ASHGKLLQEK KKLEKLHLRD LLKDEARNDL LIRSTDQGVY LDFSRQKITL ETLQHLVNLA HERQVPAMVK RMFSGEKINQ TENRAVLHVA LRMPEGSEPV HVDGKNVLDE VHAVLRRIRV FSEKVRSGEI RGHTGKKLVN VISIGIGGSY LGTEFVHLAL AAEGYAAEKA HGRQIHFLAN VDPVDVWLAE RGFDPEETLV VVISKTFTTA ETMMNARSVR DWYLHHYKGD ERALGAHFCA VSTNLDGTSK FGIQSDRVFG FWDWVGGRYS VXXAXXILPL ALQYGYDVAQ EFLNGAHAMD VHFKTAELAD NLPMLMGLIS VWNATFFGYS NVAVLPYAQA LLRFPAHIQQ LTMESNGKRV TMDGKTLDFD VGEIFFGEPG TNGQHSFYQL IHQGRVIPAE FIGFCKSQRA IKLKEEPVSN HDELMSNFFA QPDALAFGKT PEELRKEGIP EKLVPHKTFP GDRPSCMLLF PEISPFHIGQ LLALYEHRVA VEGWLWGINS FDQWGVELGK VLAKGVRGIL QKRREGKAPH ESGQSELCSS TRKILEHYVQ QS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgc cgacacagct ggaacagtgc gccagtcacg gcaagctgct gcaggagaag aaaaagctcg agaagcttca tttgcgggac ttgctcaagg acgaagcgcg aaatgacctc ctcatcagat ccactgacca gggcgtctac ctggacttta gccgccagaa aataacgctg gaaacccttc agcatcttgt gaatcttgca cacgagcgac aagtgccagc gatggtgaag cgcatgtttt cgggagagaa gatcaaccag accgagaacc gcgccgtgtt gcatgtcgcc ttgcgcatgc ccgaaggcag cgagccggtc cacgtcgatg gcaaaaatgt tctggatgaa gtgcatgcag tgctgagacg cattcgcgtc ttttccgaga aagtccgcag tggtgaaatt cgaggccaca ctgggaagaa actcgtgaac gtcatttcaa ttggcattgg cggaagctac ttggggactg agtttgttca tctcgctctc gcagccgaag gctatgccgc tgagaaggca cacggccgtc agattcactt tttagcgaac gtggacccgg tcgatgtgtg gcttgccgag agaggcttcg accctgagga gactctcgtg gttgtcatta gcaagacgtt cacgacagca gagacgatga tgaatgctcg ctctgttcgg gactggtacc tccaccacta caagggagat gaacgcgcgt tgggtgcgca cttctgcgcg gttagcacga acctggatgg cacgagcaag ttcggcattc agtcggaccg tgtcttcggc ttctgggact gggtgggcgg gaggtactcc gtcncntngg ctgnggncat tcttcctctc gctctccaat acggctatga cgtcgcgcaa gagtttctga atggcgcgca tgccatggat gttcacttca agacggccga gctggcagac aatcttccga tgctcatggg tctcatcagt gtgtggaacg ccactttctt cggctactcg aatgtcgccg ttctgcccta cgcgcaggct cttctccgct tccctgcaca catccaacag cttaccatgg agagcaacgg gaagcgcgtg acgatggatg gcaagaccct ggatttcgat gtgggcgaaa ttttctttgg agagccgggc accaacggcc agcacagttt ctaccagctc atccaccaag gtcgtgtcat tcctgcggag ttcattggct tttgcaagtc tcagagagcg atcaagctga aggaggagcc cgtaagcaac cacgacgaac tgatgagcaa cttctttgct cagccagatg cgctggcttt cggaaagaca cccgaagaac tccgcaagga aggcattccg gagaagctcg ttccccacaa gaccttccct ggcgaccgtc cttcgtgcat gcttcttttt ccggagatct cacccttcca tattggccag ctgctggcgt tgtacgagca ccgcgttgct gtggagggtt ggctgtgggg aatcaactcg ttcgatcagt ggggtgttga acttggaaag gtcctcgcca agggcgtccg tggcatcctc cagaagcgtc gtgagggcaa ggcaccccat gagtctggac aaagcgaact ctgcagctcg acgaggaaga ttttggagca ctacgtgcag cagtcga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAPTQLEQC ASHGKLLQEK KKLEKLHLRD LLKDEARNDL LIRSTDQGVY LDFSRQKITL ETLQHLVNLA HERQVPAMVK RMFSGEKINQ TENRAVLHVA LRMPEGSEPV HVDGKNVLDE VHAVLRRIRV FSEKVRSGEI RGHTGKKLVN VISIGIGGSY LGTEFVHLAL AAEGYAAEKA HGRQIHFLAN VDPVDVWLAE RGFDPEETLV VVISKTFTTA ETMMNARSVR DWYLHHYKGD ERALGAHFCA VSTNLDGTSK FGIQSDRVFG FWDWVGGRYS VTSAVGILPL ALQYGYDVAQ EFLNGAHAMD VHFKTAELAD NLPMLMGLIS VWNATFFGYS NVAVLPYAQA LLRFPAHIQQ LTMESNGKRV TMDGKTLDFD VGEIFFGEPG TNGQHSFYQL IHQGRVIPAE FIGFCKSQRA IKLKEEPVSN HDELMSNFFA QPDALAFGKT PEELRKEGIP EKLVPHKTFP GDRPSCMLLF PEISPFHIGQ LLALYEHRVA VEGWLWGINS FDQWGVELGK VLAKGVRGIL QKRREGKAPH ESGQSELCSS TRKILEHYVQ QSKA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgccgaca cagctggaac agtgcgccag tcacggcaag ctgctgcagg agaagaaaaa gctcgagaag cttcatttgc gggacttgct caaggacgaa gcgcgaaatg acctcctcat cagatccact gaccagggcg tctacctgga ctttagccgc cagaaaataa cgctggaaac ccttcagcat cttgtgaatc ttgcacacga gcgacaagtg ccagcgatgg tgaagcgcat gttttcggga gagaagatca accagaccga gaaccgcgcc gtgttgcatg tcgccttgcg catgcccgaa ggcagcgagc cggtccacgt cgatggcaaa aatgttctgg atgaagtgca tgcagtgctg agacgcattc gcgtcttttc cgagaaagtc cgcagtggtg aaattcgagg ccacactggg aagaaactcg tgaacgtcat ttcaattggc attggcggaa gctacttggg gactgagttt gttcatctcg ctctcgcagc cgaaggctat gccgctgaga aggcacacgg ccgtcagatt cactttttag cgaacgtgga cccggtcgat gtgtggcttg ccgagagagg cttcgaccct gaggagactc tcgtggttgt cattagcaag acgttcacga cagcagagac gatgatgaat gctcgctctg ttcgggactg gtacctccac cactacaagg gagatgaacg cgcgttgggt gcgcacttct gcgcggttag cacgaacctg gatggcacga gcaagttcgg cattcagtcg gaccgtgtct tcggcttctg ggactgggtg ggcgggaggt actccgtcac ctcggctgtg ggcattcttc ctctcgctct ccaatacggc tatgacgtcg cgcaagagtt tctgaatggc gcgcatgcca tggatgttca cttcaagacg gccgagctgg cagacaatct tccgatgctc atgggtctca tcagtgtgtg gaacgccact ttcttcggct actcgaatgt cgccgttctg ccctacgcgc aggctcttct ccgcttccct gcacacatcc aacagcttac catggagagc aacgggaagc gcgtgacgat ggatggcaag accctggatt tcgatgtggg cgaaattttc tttggagagc cgggcaccaa cggccagcac agtttctacc agctcatcca ccaaggtcgt gtcattcctg cggagttcat tggcttttgc aagtctcaga gagcgatcaa gctgaaggag gagcccgtaa gcaaccacga cgaactgatg agcaacttct ttgctcagcc agatgcgctg gctttcggaa agacacccga agaactccgc aaggaaggca ttccggagaa gctcgttccc cacaagacct tccctggcga ccgtccttcg tgcatgcttc tttttccgga gatctcaccc ttccatattg gccagctgct ggcgttgtac gagcaccgcg ttgctgtgga gggttggctg tggggaatca actcgttcga tcagtggggt gttgaacttg gaaaggtcct cgccaagggc gtccgtggca tcctccagaa gcgtcgtgag ggcaaggcac cccatgagtc tggacaaagc gaactctgca gctcgacgag gaagattttg gagcactacg tgcagcagtc gaaggctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for TogoA.17127.a.A1.PW28176
| PURIFICATION DATe: | 3/22/2010 |
| CONCENTRATION: | 26.2mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAPTQLEQC ASHGKLLQEK KKLEKLHLRD LLKDEARNDL LIRSTDQGVY LDFSRQKITL ETLQHLVNLA HERQVPAMVK RMFSGEKINQ TENRAVLHVA LRMPEGSEPV HVDGKNVLDE VHAVLRRIRV FSEKVRSGEI RGHTGKKLVN VISIGIGGSY LGTEFVHLAL AAEGYAAEKA HGRQIHFLAN VDPVDVWLAE RGFDPEETLV VVISKTFTTA ETMMNARSVR DWYLHHYKGD ERALGAHFCA VSTNLDGTSK FGIQSDRVFG FWDWVGGRYS VXXAXXILPL ALQYGYDVAQ EFLNGAHAMD VHFKTAELAD NLPMLMGLIS VWNATFFGYS NVAVLPYAQA LLRFPAHIQQ LTMESNGKRV TMDGKTLDFD VGEIFFGEPG TNGQHSFYQL IHQGRVIPAE FIGFCKSQRA IKLKEEPVSN HDELMSNFFA QPDALAFGKT PEELRKEGIP EKLVPHKTFP GDRPSCMLLF PEISPFHIGQ LLALYEHRVA VEGWLWGINS FDQWGVELGK VLAKGVRGIL QKRREGKAPH ESGQSELCSS TRKILEHYVQ QS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgc cgacacagct ggaacagtgc gccagtcacg gcaagctgct gcaggagaag aaaaagctcg agaagcttca tttgcgggac ttgctcaagg acgaagcgcg aaatgacctc ctcatcagat ccactgacca gggcgtctac ctggacttta gccgccagaa aataacgctg gaaacccttc agcatcttgt gaatcttgca cacgagcgac aagtgccagc gatggtgaag cgcatgtttt cgggagagaa gatcaaccag accgagaacc gcgccgtgtt gcatgtcgcc ttgcgcatgc ccgaaggcag cgagccggtc cacgtcgatg gcaaaaatgt tctggatgaa gtgcatgcag tgctgagacg cattcgcgtc ttttccgaga aagtccgcag tggtgaaatt cgaggccaca ctgggaagaa actcgtgaac gtcatttcaa ttggcattgg cggaagctac ttggggactg agtttgttca tctcgctctc gcagccgaag gctatgccgc tgagaaggca cacggccgtc agattcactt tttagcgaac gtggacccgg tcgatgtgtg gcttgccgag agaggcttcg accctgagga gactctcgtg gttgtcatta gcaagacgtt cacgacagca gagacgatga tgaatgctcg ctctgttcgg gactggtacc tccaccacta caagggagat gaacgcgcgt tgggtgcgca cttctgcgcg gttagcacga acctggatgg cacgagcaag ttcggcattc agtcggaccg tgtcttcggc ttctgggact gggtgggcgg gaggtactcc gtcncntngg ctgnggncat tcttcctctc gctctccaat acggctatga cgtcgcgcaa gagtttctga atggcgcgca tgccatggat gttcacttca agacggccga gctggcagac aatcttccga tgctcatggg tctcatcagt gtgtggaacg ccactttctt cggctactcg aatgtcgccg ttctgcccta cgcgcaggct cttctccgct tccctgcaca catccaacag cttaccatgg agagcaacgg gaagcgcgtg acgatggatg gcaagaccct ggatttcgat gtgggcgaaa ttttctttgg agagccgggc accaacggcc agcacagttt ctaccagctc atccaccaag gtcgtgtcat tcctgcggag ttcattggct tttgcaagtc tcagagagcg atcaagctga aggaggagcc cgtaagcaac cacgacgaac tgatgagcaa cttctttgct cagccagatg cgctggcttt cggaaagaca cccgaagaac tccgcaagga aggcattccg gagaagctcg ttccccacaa gaccttccct ggcgaccgtc cttcgtgcat gcttcttttt ccggagatct cacccttcca tattggccag ctgctggcgt tgtacgagca ccgcgttgct gtggagggtt ggctgtgggg aatcaactcg ttcgatcagt ggggtgttga acttggaaag gtcctcgcca agggcgtccg tggcatcctc cagaagcgtc gtgagggcaa ggcaccccat gagtctggac aaagcgaact ctgcagctcg acgaggaaga ttttggagca ctacgtgcag cagtcga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAPTQLEQC ASHGKLLQEK KKLEKLHLRD LLKDEARNDL LIRSTDQGVY LDFSRQKITL ETLQHLVNLA HERQVPAMVK RMFSGEKINQ TENRAVLHVA LRMPEGSEPV HVDGKNVLDE VHAVLRRIRV FSEKVRSGEI RGHTGKKLVN VISIGIGGSY LGTEFVHLAL AAEGYAAEKA HGRQIHFLAN VDPVDVWLAE RGFDPEETLV VVISKTFTTA ETMMNARSVR DWYLHHYKGD ERALGAHFCA VSTNLDGTSK FGIQSDRVFG FWDWVGGRYS VTSAVGILPL ALQYGYDVAQ EFLNGAHAMD VHFKTAELAD NLPMLMGLIS VWNATFFGYS NVAVLPYAQA LLRFPAHIQQ LTMESNGKRV TMDGKTLDFD VGEIFFGEPG TNGQHSFYQL IHQGRVIPAE FIGFCKSQRA IKLKEEPVSN HDELMSNFFA QPDALAFGKT PEELRKEGIP EKLVPHKTFP GDRPSCMLLF PEISPFHIGQ LLALYEHRVA VEGWLWGINS FDQWGVELGK VLAKGVRGIL QKRREGKAPH ESGQSELCSS TRKILEHYVQ QSKA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGCCGACA CAGCTGGAAC AGTGCGCCAG TCACGGCAAG CTGCTGCAGG AGAAGAAAAA GCTCGAGAAG CTTCATTTGC GGGACTTGCT CAAGGACGAA GCGCGAAATG ACCTCCTCAT CAGATCCACT GACCAGGGCG TCTACCTGGA CTTTAGCCGC CAGAAAATAA CGCTGGAAAC CCTTCAGCAT CTTGTGAATC TTGCACACGA GCGACAAGTG CCAGCGATGG TGAAGCGCAT GTTTTCGGGA GAGAAGATCA ACCAGACCGA GAACCGCGCC GTGTTGCATG TCGCCTTGCG CATGCCCGAA GGCAGCGAGC CGGTCCACGT CGATGGCAAA AATGTTCTGG ATGAAGTGCA TGCAGTGCTG AGACGCATTC GCGTCTTTTC CGAGAAAGTC CGCAGTGGTG AAATTCGAGG CCACACTGGG AAGAAACTCG TGAACGTCAT TTCAATTGGC ATTGGCGGAA GCTACTTGGG GACTGAGTTT GTTCATCTCG CTCTCGCAGC CGAAGGCTAT GCCGCTGAGA AGGCACACGG CCGTCAGATT CACTTTTTAG CGAACGTGGA CCCGGTCGAT GTGTGGCTTG CCGAGAGAGG CTTCGACCCT GAGGAGACTC TCGTGGTTGT CATTAGCAAG ACGTTCACGA CAGCAGAGAC GATGATGAAT GCTCGCTCTG TTCGGGACTG GTACCTCCAC CACTACAAGG GAGATGAACG CGCGTTGGGT GCGCACTTCT GCGCGGTTAG CACGAACCTG GATGGCACGA GCAAGTTCGG CATTCAGTCG GACCGTGTCT TCGGCTTCTG GGACTGGGTG GGCGGGAGGT ACTCCGTCAC CTCGGCTGTG GGCATTCTTC CTCTCGCTCT CCAATACGGC TATGACGTCG CGCAAGAGTT TCTGAATGGC GCGCATGCCA TGGATGTTCA CTTCAAGACG GCCGAGCTGG CAGACAATCT TCCGATGCTC ATGGGTCTCA TCAGTGTGTG GAACGCCACT TTCTTCGGCT ACTCGAATGT CGCCGTTCTG CCCTACGCGC AGGCTCTTCT CCGCTTCCCT GCACACATCC AACAGCTTAC CATGGAGAGC AACGGGAAGC GCGTGACGAT GGATGGCAAG ACCCTGGATT TCGATGTGGG CGAAATTTTC TTTGGAGAGC CGGGCACCAA CGGCCAGCAC AGTTTCTACC AGCTCATCCA CCAAGGTCGT GTCATTCCTG CGGAGTTCAT TGGCTTTTGC AAGTCTCAGA GAGCGATCAA GCTGAAGGAG GAGCCCGTAA GCAACCACGA CGAACTGATG AGCAACTTCT TTGCTCAGCC AGATGCGCTG GCTTTCGGAA AGACACCCGA AGAACTCCGC AAGGAAGGCA TTCCGGAGAA GCTCGTTCCC CACAAGACCT TCCCTGGCGA CCGTCCTTCG TGCATGCTTC TTTTTCCGGA GATCTCACCC TTCCATATTG GCCAGCTGCT GGCGTTGTAC GAGCACCGCG TTGCTGTGGA GGGTTGGCTG TGGGGAATCA ACTCGTTCGA TCAGTGGGGT GTTGAACTTG GAAAGGTCCT CGCCAAGGGC GTCCGTGGCA TCCTCCAGAA GCGTCGTGAG GGCAAGGCAC CCCATGAGTC TGGACAAAGC GAACTCTGCA GCTCGACGAG GAAGATTTTG GAGCACTACG TGCAGCAGTC GAAGGCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA