TogoA.17110.a

GPI-anchor transamidase, putative (EC 3.4.22.34)

CENTER ID:	TogoA.17110.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.17110.a.MB2.GE30439	mature protein		36	604

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_252440
OrthoMCL:	OG5_128053
RefSeq:	XP_002369352.1
UniProt:	Q8MPF3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MATSEPLAPA AASSSSSPSS FSSSSFSASL ASLCAASASP VASGSSHISP RLRFFLFSLL SLCLSSPVCF SRASSAPSAS SRSSSSPSLS SFFSGDFRNN WAVIVNTSRY WYNYRHTANA LSIYHTVKRL GIPDSQIILM LSDDHACSPR NFFPGRIFND HTRTLNLYGA GDRSGGGSSV EVDYRGDEVQ VATLLQLLAG RHNPATPRGK RLLTDENSQV LLYLSGHGGD GFLKFQDWEE ISSVDLADAV AQMKAQRRFR EMLLIAETCQ GSTLLDAMAT AGVLGLASSG PKESSYSHHA DGFLGVAVID RWTYYTLQFF EKSVRDASSS ATFEQLMNSY SRKQLLSTAS VRTELFGRPL GETKLTEFFA TASSLHATHG LYPIKTQRRV SWRMCRDRPA QDTQHKRGNS RAGGENSSER EENSIEREET ENSSEREENS SEREENSSER EENSSEREEN SSEREENSSE REENSSEREE NSSEREENSI EREENSSERG GRNAVRVGTY YERHFAITRE DRGEFDQRGR NAGKAGRTET DGQAEKAREE AGRPEAEGAS VKQAVETLVE RIWGGREEKF QSTLVAPGLM CLALGFAGVA SVLL

NT Sequence

atggcgacgt cggagcctct cgcgcccgcc gcggcttctt cttcttcttc tccttcttct ttttcttctt cttctttttc tgcttcactc gcgtctctct gcgcagcttc tgcctcgcct gtagcctctg gctcttctca catctcgcct cgtctccgtt tctttctctt ttctctcctc tctctctgtc tgtcgtcacc tgtctgcttc tctcgcgcct cgtctgctcc ctccgcctcc tcgcgttctt cttcttctcc gtctttgtcg tcgtttttct ccggcgactt tcggaacaac tgggcagtga tcgtgaacac ctcgcggtac tggtacaact accggcacac agcgaacgcg ttgtcgattt accacaccgt gaagcgcctg gggatccccg acagccagat catcttgatg ctttctgacg atcacgcctg ctcgccgcgg aacttctttc cagggcggat cttcaacgat cacacgcgga ccttgaatct ctacggagca ggcgacaggt ccggaggcgg cagcagcgtc gaggtcgact accgcggaga cgaagtccag gtcgcgactc ttctgcagct gctcgcaggg cgccacaatc ccgcgacgcc gcgcgggaag cgcctgttga ccgacgagaa cagtcaggtg ttgctgtacc tctcaggtca cggcggagac ggcttcttga agttccagga ctgggaggag atctccagtg tcgacttggc agatgctgtg gcgcagatga aggcccagcg gcgcttccga gagatgctgc tgatcgcgga aacctgccaa ggcagtacgc tgctggacgc gatggcgacg gcaggcgtcc tgggtctcgc gtcttccggt ccaaaagaga gcagctactc gcaccatgca gacggcttcc tcggcgtcgc cgtcatcgac cggtggacct actacactct ccagttcttc gaaaagtcgg tgagagacgc ctcgtcttcc gccaccttcg agcaactcat gaacagctac tcgcggaagc agctgctctc gacggcttcc gtgcgaaccg agctgttcgg caggccgctc ggggaaacca agctcacaga gttcttcgcc actgcctcca gtctgcatgc gactcacggc ctctacccaa taaaaacgca gagacgagtc tcctggagaa tgtgcagaga cagaccggcg caagacactc agcacaagcg agggaactcg cgcgcgggag gagagaactc gagcgagaga gaagagaact cgatcgagag agaagagacc gagaactcga gcgagagaga agagaactcg agcgagagag aagagaactc gagcgagaga gaagagaact cgagcgagag agaagagaac tcgagcgaga gagaagagaa ctcgagcgag agagaagaga actcgagcga gagagaagag aactcgagcg agagagaaga gaactcgatc gagagagaag agaactcgag cgagagagga gggcggaatg cagtgcgtgt ggggacctac tacgaaagac actttgcgat cacgcgtgag gacagaggag agttcgacca gagagggaga aacgctggca aggcaggaag gacagagacg gacggacagg ccgagaaggc aagagaagaa gcaggaaggc cagaggcaga aggtgcgtca gtgaagcaag ctgtcgagac ccttgtggag aggatttggg gaggacgcga agagaagttc cagtcgacgc ttgtggctcc cggattgatg tgcctagcgc tgggcttcgc gggggtcgcc tctgttcttc tgtag

Details for TogoA.17110.a.MB2.GE30439

HARVESTED ON:	5/11/2010
SEQUENCED ON:	10/7/2010
EXPECTED MW:	108kDa
OBSERVED MW:	106kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	52
PERCENT COVERAGE:	20

Validated AA Sequence

MASASPVASX XXSXXSPRLR FFLFSLLSLC XXSPXCFXXA SXAPXASXXS SSSXXSPSXX XFFSGXFXXL XXXXXXXXXX XXXXRXXXXX NXXXXXLGIP XXQXXXMLSD DHACSXXNFF XXRIFXXXXX TLNLYXAXXR SGGGSXXEVD YRXDXXQVAT LLQLLXXXXN XRDXAXXAPX XDEXXQXXLX LSXXXXRRFL KFXX

Validated NT Sequence

gnnnnnnnnn nnnnnnnnnn nnnnntcana ncnngggtcc tggttcgatg gcttctgcct cgcctgtagc ctctgnntnn nnttctnaaa tntcgcctcg tctccgtttc tttctctttt ctctcctctc tctctgtnng nngtcacctg nntgcttcnn nngngcctcg ncngctcccn ncgcntccnn nnnttcntct tcttcnncnt nttctccntc tntgnnnncg tttttctcng gngantttnn nnanctnnnn ngngannnnn nncnnntcgn gnnnnnggna cngntnanna ggaannngnn nncgntnaac nnnancnagn agngcctggg gatccccnan agncagatnn tcntgatgct ttctgacgat catgcctgct cgangnngaa cttctttcnn ngncggatct tcaanganna cnannaaacc ttgaatctct acngcgcann nnacaggtcc ggaggcggca gcancntcga ggtcgactac cgcngagacn aannccaggt cgcgactctn ctgcagctgc tcnnangnnn ccnnaatncc cgcgacnccg cncntnaagc gcctgnnnac gacgagancn nncagnnnnt gttgnanctc tcangnnann nnnngagacg nttcttgaag ttcnnngann g

Expected Protein Sequence

MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MASASPVASG SSHISPRLRF FLFSLLSLCL SSPVCFSRAS SAPSASSRSS SSPSLSSFFS GDFRNNWAVI VNTSRYWYNY RHTANALSIY HTVKRLGIPD SQIILMLSDD HACSPRNFFP GRIFNDHTRT LNLYGAGDRS GGGSSVEVDY RGDEVQVATL LQLLAGRHNP ATPRGKRLLT DENSQVLLYL SGHGGDGFLK FQDWEEISSV DLADAVAQMK AQRRFREMLL IAETCQGSTL LDAMATAGVL GLASSGPKES SYSHHADGFL GVAVIDRWTY YTLQFFEKSV RDASSSATFE QLMNSYSRKQ LLSTASVRTE LFGRPLGETK LTEFFATASS LHATHGLYPI KTQRRVSWRM CRDRPAQDTQ HKRGNSRAGG ENSSEREENS IEREETENSS EREENSSERE ENSSEREENS SEREENSSER EENSSEREEN SSEREENSSE REENSIEREE NSSERGGRNA VRVGTYYERH FAITREDRGE FDQRGRNAGK AGRTETDGQA EKAREEAGRP EAEGASVKQA VETLVERIWG GREEKFQSTL VAPGLMCLAL GFAGVASVLL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatggc ttctgcctcg cctgtagcct ctggctcttc tcacatctcg cctcgtctcc gtttctttct cttttctctc ctctctctct gtctgtcgtc acctgtctgc ttctctcgcg cctcgtctgc tccctccgcc tcctcgcgtt cttcttcttc tccgtctttg tcgtcgtttt tctccggcga ctttcggaac aactgggcag tgatcgtgaa cacctcgcgg tactggtaca actaccggca cacagcgaac gcgttgtcga tttaccacac cgtgaagcgc ctggggatcc ccgacagcca gatcatcttg atgctttctg acgatcacgc ctgctcgccg cggaacttct ttccagggcg gatcttcaac gatcacacgc ggaccttgaa tctctacgga gcaggcgaca ggtccggagg cggcagcagc gtcgaggtcg actaccgcgg agacgaagtc caggtcgcga ctcttctgca gctgctcgca gggcgccaca atcccgcgac gccgcgcggg aagcgcctgt tgaccgacga gaacagtcag gtgttgctgt acctctcagg tcacggcgga gacggcttct tgaagttcca ggactgggag gagatctcca gtgtcgactt ggcagatgct gtggcgcaga tgaaggccca gcggcgcttc cgagagatgc tgctgatcgc ggaaacctgc caaggcagta cgctgctgga cgcgatggcg acggcaggcg tcctgggtct cgcgtcttcc ggtccaaaag agagcagcta ctcgcaccat gcagacggct tcctcggcgt cgccgtcatc gaccggtgga cctactacac tctccagttc ttcgaaaagt cggtgagaga cgcctcgtct tccgccacct tcgagcaact catgaacagc tactcgcgga agcagctgct ctcgacggct tccgtgcgaa ccgagctgtt cggcaggccg ctcggggaaa ccaagctcac agagttcttc gccactgcct ccagtctgca tgcgactcac ggcctctacc caataaaaac gcagagacga gtctcctgga gaatgtgcag agacagaccg gcgcaagaca ctcagcacaa gcgagggaac tcgcgcgcgg gaggagagaa ctcgagcgag agagaagaga actcgatcga gagagaagag accgagaact cgagcgagag agaagagaac tcgagcgaga gagaagagaa ctcgagcgag agagaagaga actcgagcga gagagaagag aactcgagcg agagagaaga gaactcgagc gagagagaag agaactcgag cgagagagaa gagaactcga gcgagagaga agagaactcg atcgagagag aagagaactc gagcgagaga ggagggcgga atgcagtgcg tgtggggacc tactacgaaa gacactttgc gatcacgcgt gaggacagag gagagttcga ccagagaggg agaaacgctg gcaaggcagg aaggacagag acggacggac aggccgagaa ggcaagagaa gaagcaggaa ggccagaggc agaaggtgcg tcagtgaagc aagctgtcga gacccttgtg gagaggattt ggggaggacg cgaagagaag ttccagtcga cgcttgtggc tcccggattg atgtgcctag cgctgggctt cgcgggggtc gcctctgttc ttctgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa