TogoA.17107.a

Serine proteinase inhibitor, putative

CENTER ID:	TogoA.17107.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.17107.a.A2.GE28156	mature protein		24	294

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.17107.a.A2.PW28052	mature protein		24	294

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_208450
OrthoMCL:	OG5_162816
RefSeq:	XP_002369628.1
UniProt:	B9QKZ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGKNPLLFLA GVLVCSFSVA VFASPETKVC SCPRNLELNC GGDHVTYANH CIRECHGVGL LHDGPCADQE DQGSSVNEPS EVADTTEQPS ITPSGEEDRP PKDAPKERKR LRGCACTREL RLNCGVDGVT YSNHCVRKCE RVKLLHEGPC RGGPGRTLFS QQDDESENDA LEEEEQRLQS CPCPLNLMLN CGVDGVTYDN HCLRRCRRVE LKHEGPCEAD EQKQLPVQHG DNEIDVPERR IEDLKDCACP LILTPNCGTD RKTYPNNCVR ECAGVKLLHE GPCEGDTEEI WDDQ

NT Sequence

atgggaaaga atcctctttt gtttcttgcc ggagttctgg tttgttcgtt cagcgtagct gtgtttgctt cgcccgaaac gaaagtttgt tcttgtccgc gcaatctcga attaaactgc ggtggggatc acgtgaccta cgcaaaccac tgcatccggg agtgccacgg cgtcggcctg ttacacgacg ggccttgcgc tgaccaagaa gaccaaggtt cgtctgtcaa cgaaccatcc gaagtagccg atacaacgga acagcccagc ataacgccat cgggagagga ggatcgtccg ccaaaggacg ccccaaagga acggaagagg ctgcgaggat gcgcttgcac tcgcgaattg cgactcaact gcggtgtgga cggcgtcacg tattcaaacc attgtgtccg caaatgcgaa agagtcaagc tgctacacga aggtccttgc aggggaggtc ccggacgcac actgttttcg cagcaggatg acgaatctga gaacgatgct ctggaagagg aggaacaaag attacagagc tgcccatgcc ctcttaacct gatgcttaac tgcggcgtcg acggcgtcac gtatgacaac cactgccttc gacggtgcag gagagttgaa ctgaagcacg agggtccatg tgaagccgac gaacagaaac agcttcccgt gcagcacggt gacaatgaga tcgacgtgcc agaacggaga attgaagatc ttaaagactg cgcttgcccc ttgatcctaa cgccaaactg cgggacggac cggaagactt accccaacaa ctgtgtgcgt gagtgtgctg gagtcaaact gcttcacgag ggcccctgcg aaggggacac cgaagagatc tgggatgacc aataa

Details for TogoA.17107.a.A2.GE28156

HARVESTED ON:	12/22/2009
SEQUENCED ON:	12/31/2009
EXPECTED MW:	32kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMSPETKV CSCPRNLELN CGGDHVTYAN HCIRECHGVG LLHDGPCADQ EDQGSSVNEP SEVADTTEQP SITPSGEEDR PPKDAPKERK RLRGCACTRE LRLNCGVDGV TYSNHCVRKC ERVKLLHEGP CRGGPGRTLF SQQDDESEND ALEEEEQKLQ SCPCPLNLML NCGVDGVTYD NHCLRRCRRV ELKHEGPCEA DEQKQLPVQH GDNEIDVPER RIEDLKDCAC PLILTPNCGT DRKTYPNNCV RECAGVKLLH EGPCEGDTEE IWD

Validated NT Sequence

tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgcccgaa acgaaagttt gttcttgtcc gcgcaatctc gaattaaact gcggtgggga tcacgtgacc tacgcaaacc actgcatccg ggagtgccac ggcgtcggcc tgttacacga cgggccttgc gctgaccaag aagaccaagg ttcgtctgtc aacgaaccat ccgaagtagc cgatacaacg gaacagccca gcataacgcc atcgggagag gaggatcgtc cgccaaagga cgccccaaag gaacggaaga ggctgcgagg atgcgcttgc actcgcgaat tgcgactcaa ctgcggtgtg gacggcgtca cgtattcaaa ccattgtgtc cgcaaatgcg aaagagtcaa gctgctacac gaaggtcctt gcaggggagg tcccggacgc acactgtttt cgcagcagga tgacgaatct gagaacgatg ctctggaaga ggaggaacaa aaattacaga gctgcccatg ccctcttaac ctgatgctta actgcggcgt cgacggcgtc acgtatgaca accactgcct tcgacggtgc aggagagttg aactgaagca cgagggtcca tgtgaagccg acgaacagaa acagcttccc gtgcagcacg gtgacaatga gatcgacgtg ccagaacgga gaattgaaga tcttaaagac tgcgcttgcc ccttgatcct aacgccaaac tgcgggacgg accggaagac ttaccccaac aactgtgtgc gtgagtgtgc tggagtcaaa ctgcttcacg agggcccctg cgaaggggac accgaagaga tctgggatg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSPETKVCS CPRNLELNCG GDHVTYANHC IRECHGVGLL HDGPCADQED QGSSVNEPSE VADTTEQPSI TPSGEEDRPP KDAPKERKRL RGCACTRELR LNCGVDGVTY SNHCVRKCER VKLLHEGPCR GGPGRTLFSQ QDDESENDAL EEEEQRLQSC PCPLNLMLNC GVDGVTYDNH CLRRCRRVEL KHEGPCEADE QKQLPVQHGD NEIDVPERRI EDLKDCACPL ILTPNCGTDR KTYPNNCVRE CAGVKLLHEG PCEGDTEEIW DDQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgcccgaa acgaaagttt gttcttgtcc gcgcaatctc gaattaaact gcggtgggga tcacgtgacc tacgcaaacc actgcatccg ggagtgccac ggcgtcggcc tgttacacga cgggccttgc gctgaccaag aagaccaagg ttcgtctgtc aacgaaccat ccgaagtagc cgatacaacg gaacagccca gcataacgcc atcgggagag gaggatcgtc cgccaaagga cgccccaaag gaacggaaga ggctgcgagg atgcgcttgc actcgcgaat tgcgactcaa ctgcggtgtg gacggcgtca cgtattcaaa ccattgtgtc cgcaaatgcg aaagagtcaa gctgctacac gaaggtcctt gcaggggagg tcccggacgc acactgtttt cgcagcagga tgacgaatct gagaacgatg ctctggaaga ggaggaacaa agattacaga gctgcccatg ccctcttaac ctgatgctta actgcggcgt cgacggcgtc acgtatgaca accactgcct tcgacggtgc aggagagttg aactgaagca cgagggtcca tgtgaagccg acgaacagaa acagcttccc gtgcagcacg gtgacaatga gatcgacgtg ccagaacgga gaattgaaga tcttaaagac tgcgcttgcc ccttgatcct aacgccaaac tgcgggacgg accggaagac ttaccccaac aactgtgtgc gtgagtgtgc tggagtcaaa ctgcttcacg agggcccctg cgaaggggac accgaagaga tctgggatga ccaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for TogoA.17107.a.A2.PW28052

PURIFICATION DATe:	4/19/2010
CONCENTRATION:	24mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	100µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGCCCGAA ACGAAAGTTT GTTCTTGTCC GCGCAATCTC GAATTAAACT GCGGTGGGGA TCACGTGACC TACGCAAACC ACTGCATCCG GGAGTGCCAC GGCGTCGGCC TGTTACACGA CGGGCCTTGC GCTGACCAAG AAGACCAAGG TTCGTCTGTC AACGAACCAT CCGAAGTAGC CGATACAACG GAACAGCCCA GCATAACGCC ATCGGGAGAG GAGGATCGTC CGCCAAAGGA CGCCCCAAAG GAACGGAAGA GGCTGCGAGG ATGCGCTTGC ACTCGCGAAT TGCGACTCAA CTGCGGTGTG GACGGCGTCA CGTATTCAAA CCATTGTGTC CGCAAATGCG AAAGAGTCAA GCTGCTACAC GAAGGTCCTT GCAGGGGAGG TCCCGGACGC ACACTGTTTT CGCAGCAGGA TGACGAATCT GAGAACGATG CTCTGGAAGA GGAGGAACAA AGATTACAGA GCTGCCCATG CCCTCTTAAC CTGATGCTTA ACTGCGGCGT CGACGGCGTC ACGTATGACA ACCACTGCCT TCGACGGTGC AGGAGAGTTG AACTGAAGCA CGAGGGTCCA TGTGAAGCCG ACGAACAGAA ACAGCTTCCC GTGCAGCACG GTGACAATGA GATCGACGTG CCAGAACGGA GAATTGAAGA TCTTAAAGAC TGCGCTTGCC CCTTGATCCT AACGCCAAAC TGCGGGACGG ACCGGAAGAC TTACCCCAAC AACTGTGTGC GTGAGTGTGC TGGAGTCAAA CTGCTTCACG AGGGCCCCTG CGAAGGGGAC ACCGAAGAGA TCTGGGATGA CCAAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA