TogoA.17105.a

Rhoptry protein ROP7 (EC 2.7.11.12) (EC 2.7.11.17)

CENTER ID: TogoA.17105.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.17105.a.A3.GE28153 kinase domain 2 466 830
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_295110
OrthoMCL: OG5_149164
RefSeq: XP_002370280.1
UniProt: B6KR07
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQFEATDQAT GEPMTALVGH TVLEATARDV DSMRNEGLAV GLFQKVKNPY LANRYLRFLA PFDLVTIPGK PLVQKAKSRN EVGWVKNLLF LLPPTHVDME TFVDEIGRFP QEDRPLADAA RLYLTVQAVR LVAHLQDEGV VHGKIMPDSF CLKREGGLYL RDFGSLVRAG AKVVVPAEYD EYTPPEGRAA ARSRFGSGAT TMTYAFDAWT LGSVIFLIWC SRAPDTKSGY EYSVEFFFSR CRRVPENVKL LVYKLINPSV EARLLALQAI ETPEYREMEE QLSAASRLYS AAAFLVLGLC LVQQGAGIQR PHQWKSSEAA LSVSPAGDIV DKYSHDSTEG ENTVSEGEAE GSRGGSWLEQ EGVELRPPLL DSQTGTSTAS PTGFRRFLRR LRFWRRGSTR GSDDAAEVSR RTRVPLHTRL LQHLRRVGRF FRHGIPAAAG RFFRRVWPER PQPVFTEGDP PDLETNSLYY RDKVPGEVII REVLGKVAGF GPTSGHGVFA AYENAFSEML WAEGGAVTVI SELGRPGRQL VRGNLINIVD GGLLFQATDQ ATGEPMTVLV GSTWNKPSGK DLDKLRHQAL AIGLFQKVKN PYLANRYLRF LAPFDLVTIP GKPLVQKAKS HNEVGWVINL LLLLPAIEID MGRFVEELYE LPTEDRPLAD AARLYLTVQA VRLVAHLQDE GVVHGKILPD SFCLKREGGL YLRDFGSLVR AGTKVVAENA QGFSPPEVRG SRGGLLFGPR KTQMTHGMDA WGLGATIFFI WCFKAPTTGP EEEYSIEFLF SLCRRAPENV KLLVYKLINP SVEARLLALQ ATETPEYREM EEQLSAASRL YSGDGTPPNM GHPTSFGQPS CLVWLAAAFL VLGLCLVQQG AGIQRPHQWK SSEAALSVSP AGDIVDKYSH DSTEGENTVS EGEAEGSRGG SWLEQEGVEL TSRLLDSQAG S
NT Sequence
atgcagtttg aagcaacaga ccaggcgaca ggagaaccga tgactgcgct tgttggccat actgtgcttg aggccacggc aagagacgta gattcgatgc gcaacgaagg attagctgtt gggttgtttc aaaaggtgaa gaacccatat ctagctaaca ggtatctaag atttctggcc ccgttcgatt tggtgacgat cccggggaag ccattagttc agaaagctaa gtcacgtaat gaggttggct gggtcaaaaa tctgttgttt ctgcttcctc caactcatgt cgatatggaa acgtttgtcg acgagattgg tcgatttcca caagaggacc gccccctagc tgatgccgct cgattgtatc ttactgttca ggcggtgagg ttggtagcgc acctccaaga cgaaggagtg gtgcacggga agataatgcc tgattcgttt tgtttgaagc gtgagggggg cctgtacttg cgtgactttg gctctctggt gagagcgggc gcaaaagtgg tggtgcccgc ggaatatgat gagtatacgc cgccagaagg tcgtgcggcc gcccgaagtc gtttcggctc tggggcgacc acgatgacgt acgcattcga cgcctggaca ctgggttcgg ttatattttt aatttggtgc tctcgagctc ccgatacaaa atcggggtac gaatacagtg tcgaattctt tttttcgagg tgccggcggg tgccagagaa cgtgaaactt ctggtttaca agttgattaa cccttctgtc gaggcccgcc tgctcgcctt gcaagcgata gagactccgg aatacaggga gatggaagaa cagctgtccg ctgcttcgcg cctgtactca gctgcggcat tccttgttct ggggctttgc ctcgtccagc aaggcgctgg catacagcgg cctcaccagt ggaagagctc ggaagccgct ttgagtgtca gtccggcggg agacatcgtg gacaagtatt ctcatgattc cactgaagga gagaacactg tctcagaggg ggaagctgag ggcagtcggg ggggttcatg gctggagcag gagggggttg aattaaggcc gcctttactg gacagccaga caggaacctc gaccgcgtcc cccacaggct tcagaaggtt cctcaggcgt ttgcgttttt ggcgtcgtgg gagtacacgc ggatccgatg atgcagcaga agtttccagg aggactcggg ttcctctgca tacccgtctg cttcagcatt tgcggcgggt tggaagattt ttccgacatg gcattccagc ggcagctggc agatttttca ggcgagtttg gccggagcgt ccacaacccg ttttcactga aggtgatcct ccggatctcg agacgaattc cttgtattat cgtgacaagg ttccaggaga ggtgataatc cgggaggttc ttggcaaggt ggcggggttc ggaccaacgt ccgggcatgg tgtgtttgcc gcatatgaaa acgcattctc ggaaatgctg tgggcggaag gtggggctgt cacagtgatt tctgaattgg gccggcccgg gagacagctc gtcaggggta acctaatcaa cattgttgat ggaggattgc tgtttcaagc gacagaccaa gcgacaggag aaccgatgac tgtactggtt ggcagtactt ggaacaaacc gtctgggaag gacttagata agctgcggca ccaagcacta gctattgggt tgtttcaaaa ggtgaagaac ccatatctag ctaacaggta tctaagattt ctggccccgt tcgatttggt gacgatcccg gggaagccat tagttcagaa agctaagtca cataatgagg ttggctgggt cataaatctg ttgttactgc ttcctgcaat tgagatcgat atgggaaggt ttgtcgagga gctgtacgag cttccaacag aggaccgccc cctagctgat gccgctcgat tgtatcttac tgttcaggcg gtgaggctgg tagctcacct ccaagacgaa ggagtggtgc acgggaagat attgcctgat tcgttttgtt tgaagcgtga ggggggcctg tacttgcgtg actttggctc tctggtgaga gcgggcacaa aagtggtggc tgaaaatgct caaggctttt cgccgccgga agttcgtgga tcccgcggag gacttctttt tggtccacgc aagacccaga tgacacacgg aatggatgcg tgggggttgg gagcgactat attttttatt tggtgcttta aagctccaac tacaggacct gaagaggaat actccatcga attcttgttc tcattgtgcc ggcgggcacc agagaacgtg aaactcctgg tttacaagtt gattaaccct tctgtcgagg cccgcctgct cgccttgcaa gcgacggaga ctccggaata cagggagatg gaagaacagc tgtccgctgc ttcgcgcctg tactcaggtg atgggactcc tcccaacatg gggcacccta cctctttcgg acagccgtcg tgtcttgtct ggctagctgc ggcattcctt gttctggggc tttgcctcgt ccagcaaggc gctggcatac agcggcctca ccagtggaag agctcggaag ccgctttgag tgtcagtccg gcgggagaca tcgtggacaa gtattctcat gattccactg aaggagagaa cactgtctca gagggggaag ctgagggcag tcgggggggt tcatggctgg agcaggaggg ggttgaatta acgtcgcgtt tactggacag ccaggcagga tcgtag
Details for TogoA.17105.a.A3.GE28153
HARVESTED ON: 12/22/2009
SEQUENCED ON: 12/31/2009
EXPECTED MW: 42kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MNSLYYRDKV PGEVIIREVL GKVAGFGPTS GHGVFAAYEN AFSEMLWAEG GAVTVISELG RPGRQLVRGN LINIVDGGLL FQATDQATGE PMTVLVGSTW NKPSGKDLDK LRHQALAIGL FQKVKNPYLA NRYLRFLAPF DLVTIPGKPL VQKAKSHNEV GWVINLLLLL PAIEIDMGRF VEELYELPTE DRPLADAARL YLTVQAVRLV AHLQDEGVVH GKILPDSFCL KREGGLYLRD FGSLVRAGTK VVAENAQGFS PPEVRGSRGG LLFGPRKTQM THGMDAWGLG ATIFFIWCFK APTTGPEEEY SIEFLFSLCR RAPENVKLLV YKLINPSVEA RLLALQATET PEYREMEEQL SAASRL
Validated NT Sequence
accctggaag ctcagaccca gggtcctggt tcgatgaatt ccttgtatta tcgtgacaag gttccaggag aggtgataat ccgggaggtt cttggcaagg tggcggggtt cggaccaacg tccgggcatg gtgtgtttgc cgcatatgaa aacgcattct cggaaatgct gtgggcggaa ggtggggctg tcacagtgat ttctgaattg ggccggcccg ggagacagct cgtcaggggt aacctaatca acattgttga tggaggattg ctgtttcaag cgacagacca agcgacagga gaaccgatga ctgtactggt tggcagtact tggaacaaac cgtctgggaa ggacttagat aagctgcggc accaagcact agctattggg ttgtttcaaa aggtgaagaa cccatatcta gctaacaggt atctaagatt tctggccccg ttcgatttgg tgacgatccc ggggaagcca ttagttcaga aagctaagtc acataatgag gttggctggg tcataaatct gttgttactg cttcctgcaa ttgagatcga tatgggaagg tttgtcgagg agctgtacga gcttccaaca gaggaccgcc ccctagctga tgccgctcga ttgtatctta ctgttcaggc ggtgaggctg gtagctcacc tccaagacga aggagtggtg cacgggaaga tattgcctga ttcgttttgt ttgaagcgtg aggggggcct gtacttgcgt gactttggct ctctggtgag agcgggcaca aaagtggtgg ctgaaaatgc tcaaggcttt tcgccgccgg aagttcgtgg atcccgcgga ggacttcttt ttggtccacg caagacccag atgacacacg gaatggatgc gtgggggttg ggagcgacta tattttttat ttggtgcttt aaagctccaa ctacaggacc tgaagaggaa tactccatcg aattcttgtt ctcattgtgc cggcgggcac cagagaacgt gaaactcctg gtttacaagt tgattaaccc ttctgtcgag gcccgcctgc tcgccttgca agcgacggag actccggaat acagggagat ggaagaacag ctgtccgctg cttcgcgcct gt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNSLYYRDK VPGEVIIREV LGKVAGFGPT SGHGVFAAYE NAFSEMLWAE GGAVTVISEL GRPGRQLVRG NLINIVDGGL LFQATDQATG EPMTVLVGST WNKPSGKDLD KLRHQALAIG LFQKVKNPYL ANRYLRFLAP FDLVTIPGKP LVQKAKSHNE VGWVINLLLL LPAIEIDMGR FVEELYELPT EDRPLADAAR LYLTVQAVRL VAHLQDEGVV HGKILPDSFC LKREGGLYLR DFGSLVRAGT KVVAENAQGF SPPEVRGSRG GLLFGPRKTQ MTHGMDAWGL GATIFFIWCF KAPTTGPEEE YSIEFLFSLC RRAPENVKLL VYKLINPSVE ARLLALQATE TPEYREMEEQ LSAASRL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaattccttg tattatcgtg acaaggttcc aggagaggtg ataatccggg aggttcttgg caaggtggcg gggttcggac caacgtccgg gcatggtgtg tttgccgcat atgaaaacgc attctcggaa atgctgtggg cggaaggtgg ggctgtcaca gtgatttctg aattgggccg gcccgggaga cagctcgtca ggggtaacct aatcaacatt gttgatggag gattgctgtt tcaagcgaca gaccaagcga caggagaacc gatgactgta ctggttggca gtacttggaa caaaccgtct gggaaggact tagataagct gcggcaccaa gcactagcta ttgggttgtt tcaaaaggtg aagaacccat atctagctaa caggtatcta agatttctgg ccccgttcga tttggtgacg atcccgggga agccattagt tcagaaagct aagtcacata atgaggttgg ctgggtcata aatctgttgt tactgcttcc tgcaattgag atcgatatgg gaaggtttgt cgaggagctg tacgagcttc caacagagga ccgcccccta gctgatgccg ctcgattgta tcttactgtt caggcggtga ggctggtagc tcacctccaa gacgaaggag tggtgcacgg gaagatattg cctgattcgt tttgtttgaa gcgtgagggg ggcctgtact tgcgtgactt tggctctctg gtgagagcgg gcacaaaagt ggtggctgaa aatgctcaag gcttttcgcc gccggaagtt cgtggatccc gcggaggact tctttttggt ccacgcaaga cccagatgac acacggaatg gatgcgtggg ggttgggagc gactatattt tttatttggt gctttaaagc tccaactaca ggacctgaag aggaatactc catcgaattc ttgttctcat tgtgccggcg ggcaccagag aacgtgaaac tcctggttta caagttgatt aacccttctg tcgaggcccg cctgctcgcc ttgcaagcga cggagactcc ggaatacagg gagatggaag aacagctgtc cgctgcttcg cgcctgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa