TogoA.17098.b

Membrane skeletal protein IMC1

CENTER ID: TogoA.17098.b
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
TogoA.17098.b.A13.GE31420 full length 1 486
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_231640
OrthoMCL: OG5_156798
RefSeq: XP_002368045.1
UniProt: Q962H9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFKDCADPCS DCCQPAEQQR GQATLPSHVV LPQTSDSPAI HVTAEASQRL SSPRALQQLH GTQESVRSVT LEERADRSRT VPIGEETERQ WVAITAYQPI DTVTKTVEVP VVRTVETYVP KVVVEEKIVE VPKYIPEYVE KVVEVPEVQY VDKIVEVPEY QYEYKYVPKI ETKENIIQRP KYETKYIEKV VEVAQVKEVV RYQDVEEVEE IIRYVPKDSV VPEEWKRIQE EQDKREAELK EKEAKEEEEL KGLLEEERKL HEERVREQIK VQQQIIQQQL SARAEQERLL KERREAEMVH FPAVEGAPPL PTIPKVEQVF KPKVIKQVEI QKHVPISVDV PVPYMVPKPV VVSVQVPVLK FRDHFVPVPV RRRVVPRIRW TNEVYEVECI KEKPFLQVQD VIKPVPCDVE IRVHEFVERA APINPAELSQ ADIHAMWMRV NADLAEKRKQ ELGDKYPYVK HPAGTVFGEP GCSEEEGEVE SAQAEEGGQP AGAEPLALHP GHPLNMTYLQ NEWIKKSTVT THEMYTPEWF EAHQKALFNL TMQQPTQVQL SAEQAAKLQQ QEAQFGDAPW IEEAHAGVPM KPAAPEPEAA QCCVMCAVCG GDGVCRCQC
NT Sequence
atgtttaagg actgcgccga tccttgcagc gattgctgcc agcctgctga acagcagcgc ggccaggcca cccttccctc gcacgtggtc ctacctcaga cgtccgacag cccggccatc cacgtgactg ccgaagcttc tcagcgactc agctcgccca gagctctgca acagctccac ggcacccagg aatctgtccg atctgtcact cttgaagagc gcgctgatcg gtcacgaacc gtccccatcg gtgaagagac tgagagacaa tgggttgcca tcactgcata ccagcctatt gacaccgtga cgaagacggt cgaggtgccg gttgtgcgca ctgtggagac ctacgtccct aaggtcgtcg tcgaagagaa gatcgtcgag gtgcccaaat atatccccga atacgttgag aaggtggttg aggtacctga ggttcagtac gtcgacaaaa tcgtcgaggt tcccgagtac cagtacgagt acaagtatgt ccctaagatt gagaccaagg aaaacattat ccagcgcccg aagtatgaga ccaagtacat cgagaaggtc gttgaggtcg cccaggtcaa ggaagtcgtc cgctaccagg atgtcgagga agtggaggaa attattcgct acgtgcccaa ggactctgtt gttcctgagg aatggaagcg catccaagag gagcaggaca agcgtgaggc agagttgaag gaaaaggagg cgaaggagga ggaagagctc aaaggcctcc tggaagagga gcgcaagcta cacgaggaga gagtccgcga gcagatcaag gtccagcagc agatcatcca gcagcaactt tctgcccgcg ccgagcagga acgcttgctc aaggagaggc gcgaggccga gatggtccac ttccccgctg tcgaaggtgc ccctcctctt cccacgatcc ccaaggtgga gcaggtcttc aagcctaagg tcatcaaaca ggtggagatt cagaagcacg tgccgatctc cgtcgacgtc cctgtgccct acatggtccc gaagcccgtt gttgtgtccg tccaggtgcc cgtgctgaag ttccgtgacc acttcgtgcc tgttccggtt cgccgccgcg tcgtgcctcg catccgctgg acaaacgaag tgtatgaggt cgagtgcatc aaggagaaac cattcttgca ggtgcaagac gtgatcaagc cagttccttg cgacgtcgag atccgtgtcc acgagtttgt cgagcgagca gccccgatca accccgcaga gctgtctcag gcagacatcc acgccatgtg gatgcgcgtc aacgccgacc tggcggagaa gcgcaagcaa gagctgggcg ataagtaccc gtacgtcaag cacccggctg gaaccgtctt cggcgagccc ggatgcagcg aggaagaggg cgaagtcgaa agcgctcagg ccgaagaagg cggccaaccg gctggcgccg aaccacttgc gctccacccg ggtcacccgc tcaacatgac ctacctccag aacgagtgga tcaagaagag cactgttacc acccacgaga tgtacactcc tgagtggttc gaggcccacc agaaggccct gttcaacttg acgatgcagc agccgactca ggtgcagctg agcgcagagc aggcagccaa gcttcagcaa caggaggccc agttcggtga cgcgccgtgg atcgaagagg cacatgctgg agtaccgatg aagcccgcag cgccagaacc ggaagctgcc cagtgctgcg tcatgtgcgc ggtctgcggc ggtgatggtg tgtgccgatg ccagtgctaa
Details for TogoA.17098.b.A13.GE31420
HARVESTED ON: 2/8/2010
SEQUENCED ON: 2/14/2011
EXPECTED MW: 58kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 37
Validated AA Sequence
MVHFPAVEGA PPLPTIPKVE QVFKPKVIKQ VEIQKHVPIS VDVPVPYMVP KPVVVSVQVP VLKFRDHFVP VPVRRRVVPR IRWTNEVYEV ECIKEKPFLQ VQDVIKPVPC DVEIRVHEFV ERAAPINPAE LSQADIHAMW MRVNADLAEK RKQELGDKYP YVKHPAGTVF GEPGCSEEEG EVESAQAEE
Validated NT Sequence
tnnttcgngc tttgttagca gccggancct cgagaagctt ggctgcagaa cttgttcgtg ctgtttatta ttcttcagcc tgtgcggatt ctacttcgcc ttcctcttcg ctgcagcccg gttcaccgaa cacagtacca gccgggtgtt tgacgtacgg atacttgtcg cctaattcct gtttacgctt ctccgctaag tcagcgttaa cgcgcatcca catagcgtgg atatcagcct ggctcaattc ggccgggttg atcggtgctg cacgttcaac aaattcatgc acacggattt caacgtcgca cggaaccggt ttgatcacgt cctgcacttg cagaaacggc ttctctttga tgcactcaac ttcataaact tcgttagtcc aacggatacg cggaactaca cggcgacgaa ctggaaccgg cacgaagtgg tcacggaact tcagaaccgg gacctgcacg ctaacaacta ccggctttgg aaccatatac ggaaccggca cgtcgacgga gatcggcacg tgcttctgga tctcgacctg tttgatcacc ttcggtttaa acacctgttc aaccttagga atggtaggca gcggcggagc gccttcaaca gcagggaagt gcaccatctc agcctcgcgg cgctctttca gtaaacgttc ctgttcagcg cgtgcgctca gctgttgctg gatgatctgt tgctgaacct taatctgttc gcggacacgt tcttcgtgca gtttacgttc ctcttctaag aganctttca gctcttcctc ttccttagct tctttctctt tcagttcagc ttcacgttta tcttgttctt cttggatacg cttccactct tccggaacta cngaatcttt agggacgtag cgganaattt cctctacttc ttctacgtcc tgataacgca ccacttcttt nanctgggcc anttcaacta ccttctcaat atatttnggt ttcgtatttc ngangctgaa tnatatnnnn nnnntcnnan cntnngnacg tnnttntnnn actngnantn nnnnnntcna cnannntnnn ntacntacng ncnggnntnn nncnnnnnnn nnnntnntnn ngnnannnnn nnnnnnnnnt nnnnnnnntt nnnnnnnnnn nnnnnnntnn nnnaac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFKDCADPC SDCCQPAEQQ RGQATLPSHV VLPQTSDSPA IHVTAEASQR LSSPRALQQL HGTQESVRSV TLEERADRSR TVPIGEETER QWVAITAYQP IDTVTKTVEV PVVRTVETYV PKVVVEEKIV EVPKYIPEYV EKVVEVPEVQ YVDKIVEVPE YQYEYKYVPK IETKENIIQR PKYETKYIEK VVEVAQVKEV VRYQDVEEVE EIIRYVPKDS VVPEEWKRIQ EEQDKREAEL KEKEAKEEEE LKGLLEEERK LHEERVREQI KVQQQIIQQQ LSARAEQERL LKERREAEMV HFPAVEGAPP LPTIPKVEQV FKPKVIKQVE IQKHVPISVD VPVPYMVPKP VVVSVQVPVL KFRDHFVPVP VRRRVVPRIR WTNEVYEVEC IKEKPFLQVQ DVIKPVPCDV EIRVHEFVER AAPINPAELS QADIHAMWMR VNADLAEKRK QELGDKYPYV KHPAGTVFGE PGCSEEEGEV ESAQAEE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttaaagat tgcgctgacc cttgttccga ctgttgccag cctgctgaac agcagcgtgg ccaagctact ctgccaagcc acgtagttct gccgcagacg tccgatagcc ctgcaattca cgtgaccgct gaggctagcc agcgtctgtc ctctccacgt gccctgcagc agctccacgg cacccaggaa tctgtgcgtt ccgtgacctt agaagaacgt gctgaccgta gccgtactgt gcctatcggc gaagagaccg aacgccagtg ggttgctatt actgcttatc agcctatcga tactgtgacc aagactgtgg aagttccagt agttcgcact gtggagacct acgttccgaa agtagtggtt gaggagaaga ttgttgaagt gcctaagtac attcctgaat acgttgagaa ggtagttgaa gtcccggaag tgcagtacgt agacaagatc gtagaggtcc cggaatacca gtacgaatac aaatacgtac cgaagattga gactaaagag aatataattc agcgtccgaa atacgaaacc aaatatattg agaaggtagt tgaagtggcc caggttaaag aagtggtgcg ttatcaggac gtagaagaag tagaggaaat tatccgctac gtccctaaag attccgtagt tccggaagag tggaagcgta tccaagaaga acaagataaa cgtgaagctg aactgaaaga gaaagaagct aaggaagagg aagagctgaa aggtctctta gaagaggaac gtaaactgca cgaagaacgt gtccgcgaac agattaaggt tcagcaacag atcatccagc aacagctgag cgcacgcgct gaacaggaac gtttactgaa agagcgccgc gaggctgaga tggtgcactt ccctgctgtt gaaggcgctc cgccgctgcc taccattcct aaggttgaac aggtgtttaa accgaaggtg atcaaacagg tcgagatcca gaagcacgtg ccgatctccg tcgacgtgcc ggttccgtat atggttccaa agccggtagt tgttagcgtg caggtcccgg ttctgaagtt ccgtgaccac ttcgtgccgg ttccagttcg tcgccgtgta gttccgcgta tccgttggac taacgaagtt tatgaagttg agtgcatcaa agagaagccg tttctgcaag tgcaggacgt gatcaaaccg gttccgtgcg acgttgaaat ccgtgtgcat gaatttgttg aacgtgcagc accgatcaac ccggccgaac tgagccaggc tgatatccac gctatgtgga tgcgcgttaa cgctgactta gcggagaagc gtaaacagga attaggcgac aagtatccgt acgtcaaaca cccggctggt actgtgttcg gtgaaccggg ctgcagcgaa gaggaaggcg aagtagaatc cgcacaggct gaagaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa