TogoA.17082.t

Rhoptry kinase family protein ROP40 (incomplete catalytic triad)

CENTER ID:	TogoA.17082.t
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.17082.t.A2.GE28101	mature protein		28	519

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_291960
OrthoMCL:	OG5_239597
RefSeq:	XP_002368538.1
UniProt:	B6KL15

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTQTLCFFIF TIECLVLVLI FQGGGHPDGT TATVFRPKTF QALGLATDSD SEPEGRGGYQ RLTESTRRKK KATGRIASLF GRGRGKGDEE ASGDEGASGG KEQPLGPPSG IPRRRRFWQK EHVEPEDAVV RAFRRFLKYR VLDVETIEGA PSQLDRSSWT PSPPFFQPGE GSSKAVEYFV KQQTPALTDE MHKLLEVVDP VMPPGRPLQM SSPSSKRTTH FERGPMLRTD FFALTVDHQP FNMRIFPLPG GFEGEQKVEQ YKKVMLNEQK VLSIFGVADG KKVSNAYHCY LPVEEVFFGK GQQVISLGPS LKMPSVVFLY PSASATLGQV AEAIKLAAQQ QGTALVAQAA QLHITLQLIK LVAITTSKGI LLRRLSLENF LLRGDGVVFL SGFSELVNEK EIFYETEDGT LVTQPPTRTS RGRRFTPADN SCDLGLVIFS LWCNGSPPER EPSGWVNLDF SSCESEVPVL VQQIICGLCR AHGYPPQNAF QTLGSHEFKQ LRQLYRDVEQ RAEVHLLVE

NT Sequence

atgactcaaa ctctgtgttt ttttatcttt acaatagaat gcctggtgct tgttttaatc tttcaggggg ggggccaccc tgacgggact accgcaaccg ttttccgacc gaagaccttc caggcactgg ggctcgccac ggattctgac agtgagccag agggaagggg cggttaccaa cgactgacag agtcgactcg gcgaaagaag aaagcgactg gacgcattgc ctctctcttt ggccgaggca gaggcaaagg ggatgaggag gcatcaggcg atgaaggcgc cagcggagga aaagaacagc cactcggtcc cccctctggt attccacggc gccgtcggtt ctggcagaaa gaacacgtgg agcccgagga tgcagtggta agggcgtttc gcaggttctt gaaatacaga gtattggatg tggaaaccat agagggagct ccctcacagt tggatcgatc gtcttggacg ccttcaccgc cgttctttca gccaggagag gggtcgtcga aagccgtcga gtatttcgtc aaacagcaga caccagcgtt aactgacgag atgcataaac tattggaagt tgtcgaccca gttatgcctc ccggcaggcc gcttcagatg tcgagtccgt cctcgaaacg aaccacccac ttcgaaaggg gccccatgct gaggacagat ttctttgcac tgacagttga ccaccagcct ttcaatatga ggatattccc gttgcctggc ggattcgaag gggaacagaa ggtggaacaa tacaaaaaag ttatgctaaa tgaacagaag gttttaagca tatttggtgt tgcggatggt aagaaagtca gcaatgccta tcactgttat cttccagtcg aagaagtctt ttttggaaag ggacagcagg tgatctcact tggtccaagc ttaaaaatgc ccagtgttgt ttttctgtac ccgagcgcaa gcgcgacgct cggtcaagtc gctgaggcga taaaactagc agctcaacag cagggaactg cacttgtcgc tcaggctgcc cagctgcaca ttacactgca actcatcaag ttggtcgcga tcaccacctc aaaagggatc ctgctaagaa ggctctcact tgaaaatttc cttttgaggg gggatggggt ggtttttttg agtggctttt ctgaactcgt aaacgaaaag gaaatttttt acgaaacaga ggatgggacc cttgttacac agccaccgac gcggacttcc cgcggaagac gtttcacccc tgcagacaac agctgtgatt taggacttgt catctttagt ttgtggtgca acggttcgcc tcctgagaga gagccaagtg gatgggtcaa cctggatttc tcgagttgcg agtcagaggt gccggtgctt gtccagcaaa taatttgcgg gttgtgcagg gcccacggct accctcctca gaacgctttc caaacccttg ggagtcacga gtttaaacaa ctgaggcaat tgtaccgaga cgtggaacaa cgtgctgagg tacatttatt agtggaatga

Details for TogoA.17082.t.A2.GE28101

HARVESTED ON:	12/22/2009
SEQUENCED ON:	12/31/2009
EXPECTED MW:	57kDa
OBSERVED MW:	57kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	59

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDGTTATVF RPKTLQALGL ATDSDSEPEG KGGYQRLTES TRRKKKATGR IASLFGRGRG KGDEEASGDE GASGGKEQPL GPPSGIPRRR RFWQKERVEP EDAVVRAFRR FLKYRVLDVE TIEGTPSQLD RSSWTPSPPF FQPGEGSSKA VEYFVKQQTP ALTDEMHKLM EVVDPVMPPG RPLQMSSPSS KRTTHFERGP MLMTDFFALT VDHQPFNMRI FPLPGGFEXE QKVEQYKKVM LNEQXXLSIF GVADGKKVSN AYHCYLPVXE VFFXKGQXXX HLVQA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggacg ggactaccgc aaccgttttc cgaccgaaga ccttacaggc actggggctc gccacggatt ctgacagtga gccagaggga aagggcggtt accaacgact gacagagtcg actcggcgaa agaagaaagc gactggacgc attgcttctc tctttggccg aggcagaggc aaaggggatg aggaggcatc aggcgatgaa ggcgccagcg gaggaaaaga acagccactc ggtcccccct ctggtattcc acggcgccgt cggttctggc agaaagaacg cgtggagccc gaggatgcag tggtaagggc gtttcgcagg ttcttgaaat acagagtatt ggatgtggaa accatagagg gaactccttc acagttggat cgatcgtctt ggacgccttc accgccgttc tttcagccag gagaggggtc gtcgaaagcc gtcgagtatt tcgtcaaaca gcagacacca gcgttaactg acgagatgca taaactaatg gaagttgtcg acccagttat gcctcccggc aggccgcttc agatgtcgag tccgtcctcg aaacgaacca cccacttcga aaggggcccc atgctgatga cagatttctt tgcactgaca gttgaccacc agcctttcaa tatgaggata ttcccgttgc ctggcggatt cgaangggaa cagaaggtgg aacaatacaa aaaagttatg ctaaatgaac agaangnttt aagcatattt ggtgttgcgg atggtaagaa agtcagcaat gcctatcact gttatcttcc agtcnaagaa gtcttttttg naaagggaca gcnnnganct cacttggtcc aagcttaaaa atgcccagtg ntgtttttct gnaccngagc gcaagcncga cgctcggnca agtcgctga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDGTTATVF RPKTFQALGL ATDSDSEPEG RGGYQRLTES TRRKKKATGR IASLFGRGRG KGDEEASGDE GASGGKEQPL GPPSGIPRRR RFWQKEHVEP EDAVVRAFRR FLKYRVLDVE TIEGAPSQLD RSSWTPSPPF FQPGEGSSKA VEYFVKQQTP ALTDEMHKLL EVVDPVMPPG RPLQMSSPSS KRTTHFERGP MLRTDFFALT VDHQPFNMRI FPLPGGFEGE QKVEQYKKVM LNEQKVLSIF GVADGKKVSN AYHCYLPVEE VFFGKGQQVI SLGPSLKMPS VVFLYPSASA TLGQVAEAIK LAAQQQGTAL VAQAAQLHIT LQLIKLVAIT TSKGILLRRL SLENFLLRGD GVVFLSGFSE LVNEKEIFYE TEDGTLVTQP PTRTSRGRRF TPADNSCDLG LVIFSLWCNG SPPEREPSGW VNLDFSSCES EVPVLVQQII CGLCRAHGYP PQNAFQTLGS HEFKQLRQLY RDVEQRAEVH LLVE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacgggact accgcaaccg ttttccgacc gaagaccttc caggcactgg ggctcgccac ggattctgac agtgagccag agggaagggg cggttaccaa cgactgacag agtcgactcg gcgaaagaag aaagcgactg gacgcattgc ctctctcttt ggccgaggca gaggcaaagg ggatgaggag gcatcaggcg atgaaggcgc cagcggagga aaagaacagc cactcggtcc cccctctggt attccacggc gccgtcggtt ctggcagaaa gaacacgtgg agcccgagga tgcagtggta agggcgtttc gcaggttctt gaaatacaga gtattggatg tggaaaccat agagggagct ccctcacagt tggatcgatc gtcttggacg ccttcaccgc cgttctttca gccaggagag gggtcgtcga aagccgtcga gtatttcgtc aaacagcaga caccagcgtt aactgacgag atgcataaac tattggaagt tgtcgaccca gttatgcctc ccggcaggcc gcttcagatg tcgagtccgt cctcgaaacg aaccacccac ttcgaaaggg gccccatgct gaggacagat ttctttgcac tgacagttga ccaccagcct ttcaatatga ggatattccc gttgcctggc ggattcgaag gggaacagaa ggtggaacaa tacaaaaaag ttatgctaaa tgaacagaag gttttaagca tatttggtgt tgcggatggt aagaaagtca gcaatgccta tcactgttat cttccagtcg aagaagtctt ttttggaaag ggacagcagg tgatctcact tggtccaagc ttaaaaatgc ccagtgttgt ttttctgtac ccgagcgcaa gcgcgacgct cggtcaagtc gctgaggcga taaaactagc agctcaacag cagggaactg cacttgtcgc tcaggctgcc cagctgcaca ttacactgca actcatcaag ttggtcgcga tcaccacctc aaaagggatc ctgctaagaa ggctctcact tgaaaatttc cttttgaggg gggatggggt ggtttttttg agtggctttt ctgaactcgt aaacgaaaag gaaatttttt acgaaacaga ggatgggacc cttgttacac agccaccgac gcggacttcc cgcggaagac gtttcacccc tgcagacaac agctgtgatt taggacttgt catctttagt ttgtggtgca acggttcgcc tcctgagaga gagccaagtg gatgggtcaa cctggatttc tcgagttgcg agtcagaggt gccggtgctt gtccagcaaa taatttgcgg gttgtgcagg gcccacggct accctcctca gaacgctttc caaacccttg ggagtcacga gtttaaacaa ctgaggcaat tgtaccgaga cgtggaacaa cgtgctgagg tacatttatt agtggaaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa