TogoA.17082.d

Rhoptry kinase family protein ROP22 (incomplete catalytic triad)

CENTER ID: TogoA.17082.d
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.17082.d.A2.GE27800 mature protein 36 611
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_207700
OrthoMCL: OG5_239260
RefSeq: XP_002369574.1
UniProt: B6KP01
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLLLLNEPS LRLPRIFTVS LCAILSWGHA AFPLFFAECG LATRNLHDTG ASSWDTDTRH IASTFNSSRG SADHSFAQRA EAVSDKSVAY WKGRIVEDGD VEVEDKIVHV AQEIVGGGDS LSAVDPTELT DVARILAATE RPKVVANADA FKEKRFAIFS AGLRTSSSGP KANTFRFLAP DRTEKFAFPG RRSADPQAVS RQLVPPANLV TRGSGETVKL KGIELTGAAR GLFVTKVSAV VRQEADGSST KNPLSSSLQT RSTQRRPWYQ MLDGFVAEVD KVYEEEVEAR LAWELRIWRH LPSSRAEQLA AEEHILIPGA LLSHSGRGSP SSRRKDAARF SRELEGKWND LLSEKVFNVF IWYPAGYRDT LESSMMHSDQ ITEPWFSSTP RDVMESITVQ MILAVANLNS YGLVHTHLRS SSFVFTPEGI VLLSDLNRVV RQGEFMDCVA DREASLNDLT ASPEERVCVH EHGYKGRDAK KKEELVGPLE AHPTVDAWRL AVILYKLWCL ESMYERSSYH QFVARQDLQR VPDSESNITF LRQNPFPMSA CRKDMPSSLQ RLLRQMFEPD PANRITSAEA VKNSSFFHDK KRAVLRKKQE RLIQKSQPAD G
NT Sequence
atgaacttgc tcttgctcaa cgaaccgtcc ctccggctcc ccaggatctt tacagtatcg ctatgtgcga tcctttcctg gggacatgct gctttccctc tgttttttgc ggaatgcgga cttgccacaa ggaacctaca tgacacaggc gccagctcct gggacacaga cacacggcac attgcgtcaa cattcaactc cagtcgtgga agcgcagacc actcgttcgc ccagcgagcg gaggctgtct cagacaaatc tgtcgcgtac tggaaaggtc gaatagtcga ggatggtgat gtcgaggtag aagacaaaat cgttcacgtg gcgcaagaaa tcgtcggggg cggcgacagt ttgtctgcgg tggacccaac ggagctgaca gatgttgcac gcatcctcgc ggcaacagaa aggccaaagg tcgtagcgaa tgcagatgcc ttcaaggaaa agcggttcgc gatcttcagc gctggtctcc gcacgtccag ctcaggtcca aaggcgaaca cattccgttt cctggctcca gaccgcactg agaagttcgc gtttcctggc cgtcgcagcg cggacccgca ggcagtatcg cggcagttgg tcccgcccgc aaacctcgtc acccgtggat cgggggaaac agtgaagcta aaaggaatag agttaaccgg tgcagctcgg ggactgtttg tgacgaaagt ttctgcagta gtcagacagg aggcagacgg gtcttcgact aagaacccgc tgtcaagctc gcttcagaca cgcagcacac agcgccgccc atggtaccag atgctcgacg gctttgtggc tgaagtagac aaagtctacg aagaagaagt ggaagctcgt ctcgcgtggg aactgaggat atggcgccac cttccgtctt ctcgtgcaga acagttagcc gcagaggaac acattctgat tccgggagct ctgctgagcc acagcggccg ggggtcgcct tcttcgcgac ggaaggacgc tgcccgcttt tcgagggagt tggagggaaa gtggaacgac cttttgtccg aaaaagtgtt caacgtgttc atatggtacc cggcggggta ccgcgacacc ctagaatcgt ctatgatgca ctcagaccaa attacggagc cgtggttcag ctccacgcct cgggacgtta tggagtcgat aaccgtccag atgattctcg ctgtggcaaa tctgaatagt tacggcctag tccatacgca tttgcgttca tccagtttcg tcttcactcc agaagggatc gttcttctgt cggatctaaa ccgcgtcgtg cgacaaggag agttcatgga ctgcgtagca gaccgtgaag cctcgctgaa tgacttgacg gcttccccag aggaacgcgt gtgcgttcac gagcacggct acaagggcag agacgcgaaa aaaaaagaag agcttgtcgg acctctggag gcacacccaa ctgtggatgc gtggcgacta gcggtgattc tctacaaact ttggtgcttg gaaagcatgt acgaacggtc ttcgtaccac cagttcgttg ctcgacaaga cctccaacgc gttccagact cggagtccaa catcaccttc ctcagacaaa atcccttccc catgtctgcc tgtcggaaag acatgccctc ttccctgcaa cgtctcctcc gccagatgtt cgagcccgac cctgcgaacc ggatcacttc ggctgaagcc gtgaagaata gttccttctt tcacgacaaa aagagagcag tgctgcgaaa aaagcaggag cggctcatcc agaaatccca accggcagac ggctag
Details for TogoA.17082.d.A2.GE27800
HARVESTED ON: 11/16/2009
SEQUENCED ON: 11/23/2009
EXPECTED MW: 66kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 88
PERCENT COVERAGE: 59
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFAECGLAT RNLHDTGASS WDTDTRHIAS TFNSNRGSAD HSFAQRAEAV SDKSVAYWKG RIVEDGDVEV EDKIVHVAQE IVGGGDSLSA VDPTELTDVA RILAATERPK VVANADAFKE KRFAIFSAGL RTSSSGPKAN TFRFLAPDRT EKFAFPGRRS ADPQAVSRQL VPPANLVTRG SGETVKLKGI ELTGAARGLF VTKVSAVVRQ EADGSSTKNP LSSSLQTRST QRRPSYQMLD GFVAEVDKVY EEXVEARLAW ELXIWRXLPS SRAEQLAAEE XXLXXGXLLX HSGXGXXLRD GRTLPAFRGS WRESGTTFXP KKCSTCSYGT RRGTATP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtttg cggaatgcgg acttgccaca aggaacctac atgacacagg cgccagctcc tgggacacag acacacggca cattgcgtca acattcaact ccaatcgtgg aagcgcagac cactcgttcg cccagcgagc ggaggctgtc tcagacaaat ctgtcgcgta ctggaaaggt cgaatagtcg aggatggtga tgtcgaggta gaagacaaaa tcgttcacgt ggcgcaagaa atcgtcggtg gcggcgacag tttgtctgcg gtggacccaa cggagctgac agatgttgca cgcatcctcg cggcaacaga aaggccaaag gtcgtagcga atgcagatgc cttcaaggaa aagcggttcg cgatcttcag cgctggtctc cgcacgtcca gctcaggtcc gaaggcgaac acattccgtt tcctggctcc agaccgcact gagaagttcg cgtttcctgg ccgtcgcagc gcggacccgc aggcagtatc gcggcagttg gtcccgcccg caaacctcgt cacccgtgga tcgggggaaa cagtgaagct aaaaggaata gagttaacgg gtgcagctcg gggactgttt gtgacgaaag tttctgcagt agtcagacag gaggcagacg ggtcttcgac taagaacccg ctgtcaagct cgcttcagac acgcagcaca cagcgccgcc catcgtacca gatgctcgac ggctttgtgg ctgaagtaga caaagtctac gaagaanaag tggaagctcg tctcgcgtgg gaactganga tatggcgccn ccttccgtct tctcgtgcag aacagttagc cgcagaggaa cncnttctga ntncnggnnc tctgctganc cacagcggnc nggggnnnnn tcttcgcgac ggaaggacgc tgcccgcttt tcgagggagt tggagggaaa gtggaacgac nttttntccg aaaaagtgtt caacgtgttc atatggtacc cggcggggta ccgcgacacc ctagaatcgt ctatgatgca ctcagaccaa attacggagc cgtggttcag ctccacgcct cgggacgtta tggagtcgat aaccgtccag atgattctcg ctgtggcaaa tctgaatagt tacggcctag tccatacgca tttgcgttca tccagtttcg tcttcactcc agaagggatc gttcttctgt cggatctaaa ccgcgtcgtg cgacaaggag agttcatgga ctgcgtagca gaccgtgaag cctcgctgaa tgacttgacg gcttccccag aggaacgcgt gtgcgttcac gagcacggct acaagggcag agacgcgaaa aaaaaagaag agcttgtcgg acctctggag gcacacccaa ctgtggatgc gtggcgacta gcggtgattc tctacaaact ttggtgcttg gaaagcatgt acgaacggtc ttcgtaccac cagttcgttg ctcgacaaga cctccaacgc attccagact cggagtccaa catcaccttc ctcagacaaa atcccttccc catgtctgcc tgtcggaaag acatgccctc ttccctgcaa cgtctcctcc gccagatgtt cgagcccgac cctgcgaacc ggatcacttc ggctgaagcc gtgaagaata gttccttctt tcacgacaaa aagagagcag tgctgcgaaa aaagcaggag cggctcatcc agaaatccca accggcag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFAECGLAT RNLHDTGASS WDTDTRHIAS TFNSSRGSAD HSFAQRAEAV SDKSVAYWKG RIVEDGDVEV EDKIVHVAQE IVGGGDSLSA VDPTELTDVA RILAATERPK VVANADAFKE KRFAIFSAGL RTSSSGPKAN TFRFLAPDRT EKFAFPGRRS ADPQAVSRQL VPPANLVTRG SGETVKLKGI ELTGAARGLF VTKVSAVVRQ EADGSSTKNP LSSSLQTRST QRRPWYQMLD GFVAEVDKVY EEEVEARLAW ELRIWRHLPS SRAEQLAAEE HILIPGALLS HSGRGSPSSR RKDAARFSRE LEGKWNDLLS EKVFNVFIWY PAGYRDTLES SMMHSDQITE PWFSSTPRDV MESITVQMIL AVANLNSYGL VHTHLRSSSF VFTPEGIVLL SDLNRVVRQG EFMDCVADRE ASLNDLTASP EERVCVHEHG YKGRDAKKKE ELVGPLEAHP TVDAWRLAVI LYKLWCLESM YERSSYHQFV ARQDLQRVPD SESNITFLRQ NPFPMSACRK DMPSSLQRLL RQMFEPDPAN RITSAEAVKN SSFFHDKKRA VLRKKQERLI QKSQPADG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttgcggaa tgcggacttg ccacaaggaa cctacatgac acaggcgcca gctcctggga cacagacaca cggcacattg cgtcaacatt caactccagt cgtggaagcg cagaccactc gttcgcccag cgagcggagg ctgtctcaga caaatctgtc gcgtactgga aaggtcgaat agtcgaggat ggtgatgtcg aggtagaaga caaaatcgtt cacgtggcgc aagaaatcgt cgggggcggc gacagtttgt ctgcggtgga cccaacggag ctgacagatg ttgcacgcat cctcgcggca acagaaaggc caaaggtcgt agcgaatgca gatgccttca aggaaaagcg gttcgcgatc ttcagcgctg gtctccgcac gtccagctca ggtccaaagg cgaacacatt ccgtttcctg gctccagacc gcactgagaa gttcgcgttt cctggccgtc gcagcgcgga cccgcaggca gtatcgcggc agttggtccc gcccgcaaac ctcgtcaccc gtggatcggg ggaaacagtg aagctaaaag gaatagagtt aaccggtgca gctcggggac tgtttgtgac gaaagtttct gcagtagtca gacaggaggc agacgggtct tcgactaaga acccgctgtc aagctcgctt cagacacgca gcacacagcg ccgcccatgg taccagatgc tcgacggctt tgtggctgaa gtagacaaag tctacgaaga agaagtggaa gctcgtctcg cgtgggaact gaggatatgg cgccaccttc cgtcttctcg tgcagaacag ttagccgcag aggaacacat tctgattccg ggagctctgc tgagccacag cggccggggg tcgccttctt cgcgacggaa ggacgctgcc cgcttttcga gggagttgga gggaaagtgg aacgaccttt tgtccgaaaa agtgttcaac gtgttcatat ggtacccggc ggggtaccgc gacaccctag aatcgtctat gatgcactca gaccaaatta cggagccgtg gttcagctcc acgcctcggg acgttatgga gtcgataacc gtccagatga ttctcgctgt ggcaaatctg aatagttacg gcctagtcca tacgcatttg cgttcatcca gtttcgtctt cactccagaa gggatcgttc ttctgtcgga tctaaaccgc gtcgtgcgac aaggagagtt catggactgc gtagcagacc gtgaagcctc gctgaatgac ttgacggctt ccccagagga acgcgtgtgc gttcacgagc acggctacaa gggcagagac gcgaaaaaaa aagaagagct tgtcggacct ctggaggcac acccaactgt ggatgcgtgg cgactagcgg tgattctcta caaactttgg tgcttggaaa gcatgtacga acggtcttcg taccaccagt tcgttgctcg acaagacctc caacgcgttc cagactcgga gtccaacatc accttcctca gacaaaatcc cttccccatg tctgcctgtc ggaaagacat gccctcttcc ctgcaacgtc tcctccgcca gatgttcgag cccgaccctg cgaaccggat cacttcggct gaagccgtga agaatagttc cttctttcac gacaaaaaga gagcagtgct gcgaaaaaag caggagcggc tcatccagaa atcccaaccg gcagacggca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a