SSGCID: Target Page

TogoA.10317.b

Protein kinase 3

CENTER ID:	TogoA.10317.b
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.10317.b.A1.GE30877	full length		1	394

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_265330
RefSeq:	XP_002368662.1
UniProt:	B9Q1F6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPDPQYDPAA AQKSSNARGG GSQRTTAVKG ERQSRVHTGN PHPTLTYDIG RVLGNGSFGV VTEARCVDTG EVVAIKKVLQ DPRYKNRELD IMKELKHPNV VKLIDYFYTE MGERDSNDHN RFLNVVMEHI PETVYRVMKS FLRANQQVPF ILIKLYTYQM CRALGYLHAL GICHRDIKPQ NLLVDSRTHV LKLCDFGSAK RLVPGEQSVS YICSRFYRAP ELMLGASEYT TAIDVWSIGC VLGELLLGRP LFAGETSVDQ LVKIIQILGT PSRRQMSTMN PNYTEFRFPD VKPREWKSIF ASHIASTETN DPSWDQALDL LTKFLRYEPG ERLLPLEALA HDFFDELRLP STRLPSGGPL PELFTFSQLE LQNMSPAAQV KCIPKHIASA NNRGX

NT Sequence

atgccggacc cgcagtacga tcccgcagct gctcagaaga gtagcaacgc tcgcggagga ggaagtcaac ggacaacagc cgtgaagggg gaacggcaga gccgcgttca cacaggcaac cctcacccga ctctgactta cgatattggg agggtcctcg gcaatggatc attcggcgtc gtcacagagg caagatgcgt tgatacgggg gaagtcgtag ctatcaagaa agtccttcag gacccgcgtt acaaaaatcg cgaacttgat attatgaagg aactcaaaca ccccaatgtt gttaaactca ttgactactt ctacacggaa atgggcgaac gcgatagcaa cgaccacaac cggttcctca acgttgtgat ggaacacata ccggaaacgg tatacagagt catgaagtct tttctacggg ctaatcaaca ggtcccgttc attctgatca agctgtacac ctaccaaatg tgccgcgcgt tgggttatct tcatgctttg ggcatttgcc accgcgacat caagcctcag aatctgttgg tagattctcg gacacatgtc ctcaagttgt gtgactttgg ttcggcaaaa cggcttgttc ccggcgaaca gagtgtctct tacatctgtt ctcggtttta ccgggcacct gagctcatgc ttggcgcgag cgaatatact acggcaatcg acgtttggtc catagggtgt gtgctcggag agctactgct tggccgccca ctgtttgccg gcgagactag tgtagaccag ttggtcaaga tcattcaaat tttgggcact cccagcaggc gtcaaatgtc aactatgaac ccgaactaca cagaatttcg gttccctgat gtcaaaccga gagagtggaa gtcgatattt gcttcgcata ttgcttcgac tgagaccaat gacccatcat gggaccaagc gctcgacctt ctgaccaagt ttttgagata tgagcctgga gagaggctcc ttccgctgga agctttagct catgatttct tcgatgagct acgtctgcca tcaacgaggc tcccgagtgg tggacctctg cccgagttgt tcaccttctc ccagctagag ctgcagaaca tgtcgcctgc agcgcaagta aaatgtattc cgaagcatat cgccagtgca aacaaccgtg gctaa

Details for TogoA.10317.b.A1.GE30877

HARVESTED ON:	11/16/2010
SEQUENCED ON:	11/23/2010
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

tgtttgcact ggcgatatgc ttcggaatac attttacttg cgctgcaggc gacatgttct gcagctctag ctgggagaag gtgaacaact cgggcagagg tccaccactc gggagcctcg ttgatggcag acgtagctca tcgaagaaat catgagctaa agcttccagc ggaaggagcc tctctccagg ctcatatctc aaaaacttgg tcagaaggtc gagcgcttgg tcccatgatg ggtcattggt ctcagtcgaa gcaatatgcg aagcaaatat cgacttccac tctctcggtt tgacatcagg gaaccgaaat tctgtgtagt tcgggttcat agttgacatt tgacgcctgc tgggagtgcc caaaatttga atgatcttga ccaactggtc tacactagtc tcgccggcaa acagtgggcg gccaagcagt agctctccga gcacacaccc tatggaccaa acgtcgattg ccgtagtata ttcgctcgcg ccaagcatga gctcaggtgc ccggtaaaac cgagaacaga tgtaagagac actctgttcg ccgggaacaa gccgttttgc cgaaccaaag tcacacaact tgaggacatg tgtccgagaa tctaccaaca gattctgagg cttgatgtcg cggtggcaaa tgcccaaagc atgaagataa cccaacgcgc ggcacatttg gtaggtgtac agcttgatca gaatgaacgg gacctgttga ttagcccgta gaaaagactt catgactctg tataccgttt ccggtatgtg ttccatcaca acgttgagga accggttgtg gtcgttgcta tcgcgttcgc ccatttccgt gtagaagtag tcaatgagtt taacaacatt ggggtgtttg agttccttca taatatcaag ttcgcgattt ttgtaacgcg ggtcctgaag gactttcttg atagctacga cttcccccgt atcaacgcat cttgcctctg tgacgacgcc gaatgatcca ttgccgagga ccctcccaat atcgtaagtc agagtcgggt gagggttgcc tgtgtgaacg cggctctgcc gttccccctt cacggctgtt gtccgttgac ttcctcctcc gcgagcgttg ctactcttct gagcagctgc gggatcgtac tgcgggtccg gcatcgaacc aggaccctgg gtctgagctt cca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPDPQYDPA AAQKSSNARG GGSQRTTAVK GERQSRVHTG NPHPTLTYDI GRVLGNGSFG VVTEARCVDT GEVVAIKKVL QDPRYKNREL DIMKELKHPN VVKLIDYFYT EMGERDSNDH NRFLNVVMEH IPETVYRVMK SFLRANQQVP FILIKLYTYQ MCRALGYLHA LGICHRDIKP QNLLVDSRTH VLKLCDFGSA KRLVPGEQSV SYICSRFYRA PELMLGASEY TTAIDVWSIG CVLGELLLGR PLFAGETSVD QLVKIIQILG TPSRRQMSTM NPNYTEFRFP DVKPREWKSI FASHIASTET NDPSWDQALD LLTKFLRYEP GERLLPLEAL AHDFFDELRL PSTRLPSGGP LPELFTFSQL ELQNMSPAAQ VKCIPKHIAS ANNRG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccggacccg cagtacgatc ccgcagctgc tcagaagagt agcaacgctc gcggaggagg aagtcaacgg acaacagccg tgaaggggga acggcagagc cgcgttcaca caggcaaccc tcacccgact ctgacttacg atattgggag ggtcctcggc aatggatcat tcggcgtcgt cacagaggca agatgcgttg atacggggga agtcgtagct atcaagaaag tccttcagga cccgcgttac aaaaatcgcg aacttgatat tatgaaggaa ctcaaacacc ccaatgttgt taaactcatt gactacttct acacggaaat gggcgaacgc gatagcaacg accacaaccg gttcctcaac gttgtgatgg aacacatacc ggaaacggta tacagagtca tgaagtcttt tctacgggct aatcaacagg tcccgttcat tctgatcaag ctgtacacct accaaatgtg ccgcgcgttg ggttatcttc atgctttggg catttgccac cgcgacatca agcctcagaa tctgttggta gattctcgga cacatgtcct caagttgtgt gactttggtt cggcaaaacg gcttgttccc ggcgaacaga gtgtctctta catctgttct cggttttacc gggcacctga gctcatgctt ggcgcgagcg aatatactac ggcaatcgac gtttggtcca tagggtgtgt gctcggagag ctactgcttg gccgcccact gtttgccggc gagactagtg tagaccagtt ggtcaagatc attcaaattt tgggcactcc cagcaggcgt caaatgtcaa ctatgaaccc gaactacaca gaatttcggt tccctgatgt caaaccgaga gagtggaagt cgatatttgc ttcgcatatt gcttcgactg agaccaatga cccatcatgg gaccaagcgc tcgaccttct gaccaagttt ttgagatatg agcctggaga gaggctcctt ccgctggaag ctttagctca tgatttcttc gatgagctac gtctgccatc aacgaggctc ccgagtggtg gacctctgcc cgagttgttc accttctccc agctagagct gcagaacatg tcgcctgcag cgcaagtaaa atgtattccg aagcatatcg ccagtgcaaa caaccgtggc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa