SSGCID: Target Page

TogoA.07054.a

Cytochrome p450 superfamily protein

CENTER ID:	TogoA.07054.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.07054.a.AE51.GE39190	P450 functional domain, full		142	553
TogoA.07054.a.AE71.GE39191	TgCYP204 with N-term modif		70	553

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.07054.a.AE71.PW37747	TgCYP204 with N-term modif		70	553

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_315770
UniProt:	S8EQL7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSELSTPSDL LRYVPTSVQD LFRNVVYWGS NDEATQRWRL RVLSIGAVAV AGAASWVYLI YPFVQHVKVW SLPQPETSTW LNGDKKELEK HVRKGDKRHY FSRLRQTVGP NCFIRLPLQF SLASLTKNPF GLLFVTSDWN VINELLRRSG SLVRNPEWVQ AFDVISDSVI AIEDEQRARL HRRILSTFTT HQATNGTMKP LMDLLEVLRK ELVVLNDVRA VDALQLTRLT MVDLWLDLLT GRQGLHAVAS SGHLALTPKR DETGVLPYST VDAMMTTSRF HKENYELLFL PTDSSGAACT ASHRALVRQY KSVVNATPVI LTQMLDETNQ YALLPRILAA EDSVTRRGLM ADEIRDTASL LLLAAENTAL PIVWALYELA KDPLLQERIY TATRMEDLTL VDSTDHLVSH LGEVVNVFLE SLRFYALPIL SRSASKAIHL KSVDFSIPAG TVILVDNYSL TRDEVLWGQD ANVFNPDRFV GRIWQQAPWL PFGFGTRKCL GERLAVAHAV IFLAFIVRHF ALDLDVNSMP PEPMERQFLT PDKPVMLRFK PRA

NT Sequence

ATGTCGGAAC TTAGTACGCC TTCGGATCTA CTCCGCTATG TCCCCACGAG CGTCCAAGAC CTCTTCAGGA ATGTTGTGTA CTGGGGGTCG AACGACGAAG CTACTCAGAG GTGGAGGTTG CGAGTTCTCA GTATCGGCGC AGTAGCCGTG GCGGGCGCCG CCTCTTGGGT GTATCTTATC TACCCCTTTG TGCAGCACGT GAAAGTGTGG AGCTTGCCCC AGCCGGAGAC GTCCACGTGG CTCAACGGCG ACAAAAAAGA GCTCGAAAAG CATGTCCGGA AAGGAGACAA ACGACATTAC TTCTCTCGTC TGCGCCAGAC AGTGGGGCCT AACTGTTTTA TTCGCCTGCC TCTTCAATTC TCTCTTGCGT CGTTGACGAA GAATCCCTTT GGCCTTCTCT TCGTCACCAG TGACTGGAAT GTGATTAATG AACTACTGAG GCGGAGCGGA AGCTTGGTGA GGAACCCTGA GTGGGTGCAG GCGTTTGACG TGATAAGCGA CAGCGTAATC GCCATCGAAG ACGAACAAAG GGCCCGCCTC CACAGAAGAA TTCTCTCGAC CTTCACCACC CACCAGGCGA CGAATGGAAC GATGAAGCCT CTTATGGATC TCCTCGAGGT CCTTCGAAAA GAGCTCGTCG TTCTTAATGA CGTTCGAGCT GTCGATGCGC TGCAGCTCAC GCGGCTGACG ATGGTTGACT TGTGGCTGGA TTTGCTTACT GGCCGTCAGG GGCTGCACGC AGTTGCTTCA TCGGGTCATC TTGCTCTGAC TCCCAAGAGA GACGAGACAG GTGTCTTGCC CTACAGCACT GTGGACGCGA TGATGACTAC GAGCAGATTT CACAAAGAGA ACTACGAACT TCTCTTCCTC CCGACAGACT CTTCTGGCGC GGCCTGTACA GCCTCACACA GAGCCCTTGT CCGCCAATAC AAATCTGTGG TCAACGCCAC CCCCGTCATT CTGACGCAGA TGCTGGACGA GACGAATCAA TATGCACTCT TGCCCAGAAT CCTCGCAGCT GAAGACTCTG TGACACGCCG TGGTCTAATG GCGGACGAAA TCCGCGATAC CGCGTCTCTG CTTTTGCTTG CGGCGGAGAA CACTGCACTG CCCATCGTCT GGGCGTTGTA CGAGCTCGCG AAAGACCCGC TGCTTCAAGA ACGCATTTAC ACTGCCACGC GGATGGAAGA CTTGACACTG GTCGACAGCA CAGATCACCT GGTTAGCCAT CTCGGAGAAG TTGTGAATGT CTTCCTCGAG AGTCTACGGT TTTACGCTCT CCCAATTCTC TCGCGAAGTG CATCCAAGGC CATTCATCTG AAATCAGTGG ATTTCTCAAT CCCCGCCGGC ACGGTTATTT TGGTGGACAA TTACTCGCTG ACGCGTGATG AGGTTCTCTG GGGACAAGAC GCCAACGTCT TCAATCCAGA TCGATTTGTG GGCAGAATAT GGCAACAGGC CCCTTGGCTG CCTTTCGGAT TTGGCACACG CAAGTGTCTC GGAGAACGAC TAGCCGTAGC CCATGCAGTT ATTTTTCTCG CCTTCATTGT TCGTCACTTC GCTCTTGACC TAGACGTGAA TTCGATGCCC CCGGAGCCAA TGGAACGTCA GTTCCTCACT CCCGACAAGC CTGTCATGCT TCGTTTCAAA CCTCGGGCGT AG

Details for TogoA.07054.a.AE51.GE39190

HARVESTED ON:	5/27/2015
SEQUENCED ON:	6/26/2015
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAKKTSSKGL INELLRRSGS LVRNPEWVQA FDVISDSVIA IEDEQRARLH RRILSTFTTH QATNGTMKPL MDLLEVLRKE LVVLNDVRAV DALQLTRLTM VDLWLDLLTG RQGLHAVASS GHLALTPKRD ETGVLPYSTV DAMMTTSRFH KENYELLFLP TDSSGAACTA SHRALVRQYK SVVNATPVIL TQMLDETNQY ALLPRILAAE DSVTRRGLMA DEIRDTASLL LLAAENTALP IVWALYELAK DPLLQERIYT ATRMEDLTLV DSTDHLVSHL GEVVNVFLES LRFYALPILS RSASKAIHLK SVDFSIPAGT VILVDNYSLT RDEVLWGQDA NVFNPDRFVG RIWQQAPWLP FGFGTRKCLG ERLAVAHAVI FLAFIVRHFA LDLDVNSMPP EPMERQFLTP DKPVMLRFKP RAGHHHHHH

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat ggcgaaaaaa accagcagca aaggcctgat taatgaacta ctgaggcgga gcggaagctt ggtgaggaac cctgagtggg tgcaggcgtt tgacgtgata agcgacagcg taatcgccat cgaagacgaa caaagggccc gcctccacag aagaattctc tcgaccttca ccacccacca ggcgacgaat ggaacgatga agcctcttat ggatctcctc gaggtccttc gaaaagagct cgtcgttctt aatgacgttc gagctgtcga tgcgctgcag ctcacgcggc tgacgatggt tgacttgtgg ctggatttgc ttactggccg tcaggggctg cacgcagttg cttcatcggg tcatcttgct ctgactccca agagagacga gacaggtgtc ttgccctaca gcactgtgga cgcgatgatg actacgagca gatttcacaa agagaactac gaacttctct tcctcccgac agactcttct ggcgcggcct gtacagcctc acacagagcc cttgtccgcc aatacaaatc tgtggtcaac gccacccccg tcattctgac gcagatgctg gacgagacga atcaatatgc actcttgccc agaatcctcg cagctgaaga ctctgtgaca cgccgtggtc taatggcgga cgaaatccgc gataccgcgt ctctgctttt gcttgcggcg gagaacactg cactgcccat cgtctgggcg ttgtacgagc tcgcgaaaga cccgctgctt caagaacgca tttacactgc cacgcggatg gaagacttga cactggtcga cagcacagat cacctggtta gccatctcgg agaagttgtg aatgtcttcc tcgagagtct acggttttac gctctcccaa ttctctcgcg aagtgcatcc aaggccattc atctgaaatc agtggatttc tcaatccccg ccggcacggt tattttggtg gacaattact cgctgacgcg tgatgaggtt ctctggggac aagacgccaa cgtcttcaat ccagatcgat ttgtgggcag aatatggcaa caggcccctt ggctgccttt cggatttggc acacgcaagt gtctcggaga acgactagcc gtagcccatg cagttatttt tctcgccttc attgttcgtc acttcgctct tgacctagac gtgaattcga tgcccccgga gccaatggaa cgtcagttcc tcactcccga caagcctgtc atgcttcgtt tcaaacctcg ggcggggcac caccatcatc atcattaacg gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at

Details for TogoA.07054.a.AE71.GE39191

HARVESTED ON:	5/27/2015
SEQUENCED ON:	6/26/2015
EXPECTED MW:	56kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAKKTSSKGL WSLPQPETST WLNGDKKELE KHVRKGDKRH YFSRLRQTVG PNCFIRLPLQ FSLASLTKNP FGLLFVTSDW NVINELLRRS GSLVRNPEWV QAFDVISDSV IAIEDEQRAR LHRRILSTFT THQATNGTMK PLMDLLEVLR KELVVLNDVR AVDALQLTRL TMVDLWLDLL TGRQGLHAVA SSGHLALTPK RDETGVLPYS TVDAMMTTSR FHKENYELLF LPTDSSGAAC TASHRALVRQ YKSVVNATPV ILTQMLDETN QYALLPRILA AEDSVTRRGL MADEIRDTAS LLLLAAENTA LPIVWALYEL AKDPLLQERI YTATRMEDLT LVDSTDHLVS HLGEVVNVFL ESLRFYALPI LSRSASKAIH LKSVDFSIPA GTVILVDNYS LTRDEVLWGQ DANVFNPDRF VGRIWQQAPW LPFGFGTRKC LGERLAVAHA VIFLAFIVRH FALDLDVNSM PPEPMERQFL TPDKPVMLRF KPRAGHHHHH H

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat ggcgaaaaaa accagcagca aaggcctgtg gagcttgccc cagccggaga cgtccacgtg gctcaacggc gacaaaaaag agctcgaaaa gcatgtccgg aaaggagaca aacgacatta cttctctcgt ctgcgccaga cagtggggcc taactgtttt attcgcctgc ctcttcaatt ctctcttgcg tcgttgacga agaatccctt tggccttctc ttcgtcacca gtgactggaa tgtgattaat gaactactga ggcggagcgg aagcttggtg aggaaccctg agtgggtgca ggcgtttgac gtgataagcg acagcgtaat cgccatcgaa gacgaacaaa gggcccgcct ccacagaaga attctctcga ccttcaccac ccaccaggcg acgaatggaa cgatgaagcc tcttatggat ctcctcgagg tccttcgaaa agagctcgtc gttcttaatg acgttcgagc tgtcgatgcg ctgcagctca cgcggctgac gatggttgac ttgtggctgg atttgcttac tggccgtcag gggctgcacg cagttgcttc atcgggtcat cttgctctga ctcccaagag agacgagaca ggtgtcttgc cctacagcac tgtggacgcg atgatgacta cgagcagatt tcacaaagag aactacgaac ttctcttcct cccgacagac tcttctggcg cggcctgtac agcctcacac agagcccttg tccgccaata caaatctgtg gtcaacgcca cccccgtcat tctgacgcag atgctggacg agacgaatca atatgcactc ttgcccagaa tcctcgcagc tgaagactct gtgacacgcc gtggtctaat ggcggacgaa atccgcgata ccgcgtctct gcttttgctt gcggcggaga acactgcact gcccatcgtc tgggcgttgt acgagctcgc gaaagacccg ctgcttcaag aacgcattta cactgccacg cggatggaag acttgacact ggtcgacagc acagatcacc tggttagcca tctcggagaa gttgtgaatg tcttcctcga gagtctacgg ttttacgctc tcccaattct ctcgcgaagt gcatccaagg ccattcatct gaaatcagtg gatttctcaa tccccgccgg cacggttatt ttggtggaca attactcgct gacgcgtgat gaggttctct ggggacaaga cgccaacgtc ttcaatccag atcgatttgt gggcagaata tggcaacagg ccccttggct gcctttcgga tttggcacac gcaagtgtct cggagaacga ctagccgtag cccatgcagt tatttttctc gccttcattg ttcgtcactt cgctcttgac ctagacgtga attcgatgcc cccggagcca atggaacgtc agttcctcac tcccgacaag cctgtcatgc ttcgtttcaa acctcgggcg gggcaccacc atcatcatca ttaacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat

Details for TogoA.07054.a.AE71.PW37747

PURIFICATION DATe:	6/23/2015
CONCENTRATION:	9mg/ml
OBSERVED MW:	56kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GGCGAAAAAA ACCAGCAGCA AAGGCCTGTG GAGCTTGCCC CAGCCGGAGA CGTCCACGTG GCTCAACGGC GACAAAAAAG AGCTCGAAAA GCATGTCCGG AAAGGAGACA AACGACATTA CTTCTCTCGT CTGCGCCAGA CAGTGGGGCC TAACTGTTTT ATTCGCCTGC CTCTTCAATT CTCTCTTGCG TCGTTGACGA AGAATCCCTT TGGCCTTCTC TTCGTCACCA GTGACTGGAA TGTGATTAAT GAACTACTGA GGCGGAGCGG AAGCTTGGTG AGGAACCCTG AGTGGGTGCA GGCGTTTGAC GTGATAAGCG ACAGCGTAAT CGCCATCGAA GACGAACAAA GGGCCCGCCT CCACAGAAGA ATTCTCTCGA CCTTCACCAC CCACCAGGCG ACGAATGGAA CGATGAAGCC TCTTATGGAT CTCCTCGAGG TCCTTCGAAA AGAGCTCGTC GTTCTTAATG ACGTTCGAGC TGTCGATGCG CTGCAGCTCA CGCGGCTGAC GATGGTTGAC TTGTGGCTGG ATTTGCTTAC TGGCCGTCAG GGGCTGCACG CAGTTGCTTC ATCGGGTCAT CTTGCTCTGA CTCCCAAGAG AGACGAGACA GGTGTCTTGC CCTACAGCAC TGTGGACGCG ATGATGACTA CGAGCAGATT TCACAAAGAG AACTACGAAC TTCTCTTCCT CCCGACAGAC TCTTCTGGCG CGGCCTGTAC AGCCTCACAC AGAGCCCTTG TCCGCCAATA CAAATCTGTG GTCAACGCCA CCCCCGTCAT TCTGACGCAG ATGCTGGACG AGACGAATCA ATATGCACTC TTGCCCAGAA TCCTCGCAGC TGAAGACTCT GTGACACGCC GTGGTCTAAT GGCGGACGAA ATCCGCGATA CCGCGTCTCT GCTTTTGCTT GCGGCGGAGA ACACTGCACT GCCCATCGTC TGGGCGTTGT ACGAGCTCGC GAAAGACCCG CTGCTTCAAG AACGCATTTA CACTGCCACG CGGATGGAAG ACTTGACACT GGTCGACAGC ACAGATCACC TGGTTAGCCA TCTCGGAGAA GTTGTGAATG TCTTCCTCGA GAGTCTACGG TTTTACGCTC TCCCAATTCT CTCGCGAAGT GCATCCAAGG CCATTCATCT GAAATCAGTG GATTTCTCAA TCCCCGCCGG CACGGTTATT TTGGTGGACA ATTACTCGCT GACGCGTGAT GAGGTTCTCT GGGGACAAGA CGCCAACGTC TTCAATCCAG ATCGATTTGT GGGCAGAATA TGGCAACAGG CCCCTTGGCT GCCTTTCGGA TTTGGCACAC GCAAGTGTCT CGGAGAACGA CTAGCCGTAG CCCATGCAGT TATTTTTCTC GCCTTCATTG TTCGTCACTT CGCTCTTGAC CTAGACGTGA ATTCGATGCC CCCGGAGCCA ATGGAACGTC AGTTCCTCAC TCCCGACAAG CCTGTCATGC TTCGTTTCAA ACCTCGGGCG GGGCACCACC ATCATCATCA TTAACGGATC CGAATTCGAG CTCCGTCGAC AAGCTTGCGG CCGCACTCGA GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGAT