TogoA.01236.a

Fructose-bisphosphate aldolase (EC 4.1.2.13)

CENTER ID: TogoA.01236.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
TogoA.01236.a.A11.GE33353 full length 71 433
TogoA.01236.a.A12.GE33354 full length 71 410
TogoA.01236.a.A13.GE33355 full length 71 419
TogoA.01236.a.A15.GE33357 full length 78 410
TogoA.01236.a.A16.GE33358 full length 78 419
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
TogoA.01236.a.A13.PS01397 full length 71 419
TogoA.01236.a.A15.PW36055 full length 78 410

Structures

4TU1
DEPOSITED: 6/23/2014
DETERMINATION: XRay
CLONE: TogoA.01236.a.A15.GE33357
PROTEIN: TogoA.01236.a.A15.PC00111

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_236040
OrthoMCL: OG5_127143
RefSeq: XP_002368989.1
UniProt: B9PW35

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLCIVERIAM PTRGVNPSAA NGKLPPRTRS ATGCRVYIHP MVKFISLLFA VFYARFFLLF CVRIQSTFPT MSGYGLPISQ EVAKELAENA RKIAAPGKGI LAADESTGTI KKRFDSIGVE NTEANRAFYR DLLFSTKGLG QYISGAILFE ETLYQKSPSG VPMVDLLKAE GIIPGIKVDK GLETLPLTDD EKATMGLDGL SERCKKYYEA GARFAKWRAV LSIDPAKGKP TNLSITEVAH GLARYAAICQ ANRLVPIVEP EILTDGSHDI TVCAEVTERV LAAVFKALND HHVLLEGALL KPNMVTHGSD CPKPASHEEI AFYTVRSLKR TVPPALPGVM FLSGGQSEED ASLNLNEMNK MGPHPFQLSF SYGRALQASC LKAWKGVPEN KAKAQQVLME RARANGEAQL GKYGGGAGGA AAASSLFEKR YVY
NT Sequence
atgctttgca ttgtagagcg aattgcaatg ccaacgcgtg gtgtcaaccc ctcggcggca aacggcaagc tcccgccgcg gacccggtcc gcaacgggat gcagagtgta catacacccg atggtaaagt tcatatcctt actttttgcc gttttttacg cacgattctt tctgcttttt tgtgtacgca ttcaatccac atttcccacc atgtcgggat acggtcttcc catttcccag gaggtggcca aggagctggc cgagaacgcc cggaagattg cagcgccggg caagggtatt cttgcagccg atgaatccac tggcaccatc aagaagcgct tcgacagcat cggagttgag aacaccgagg cgaaccgcgc gttctaccgt gacttgcttt tcagcactaa gggtctcggc cagtacatta gcggcgcgat ccttttcgaa gagactctgt accagaagag cccgtcgggc gtgccgatgg tagacctgtt gaaggccgag gggatcatcc cgggcatcaa ggtcgacaag ggcctggaga ctcttccgct gacggacgac gagaaggcga ctatgggttt ggacggcctc tctgagcgct gcaagaagta ctacgaggca ggcgctcgtt tcgccaagtg gcgtgcggtg ctcagcatcg acccggcaaa gggaaagcct acaaatttgt ctatcactga ggtggcccac ggcctcgcgc gctacgcggc aatctgccaa gcgaatcgac tcgtccctat cgtcgaaccc gagattctca cagacggcag ccacgacatc accgtgtgcg ccgaagtcac cgagcgtgtg ctagcggcgg tcttcaaggc tctgaacgac caccacgttc tgctggaggg agcgctactg aagccaaaca tggtgaccca cggctcggac tgcccgaagc cggcgtctca cgaggagatt gctttctaca ccgtccgcag cctgaagcgc acggtcccgc ctgcgctccc cggcgtcatg tttctctccg gaggccagtc cgaggaagac gcttcgctga acctgaacga gatgaacaag atgggacccc acccgttcca gctctccttc tcttacggcc gcgctctcca ggcttcctgc ctgaaggcct ggaagggtgt ccccgaaaac aaagccaagg cacagcaagt gctgatggag cgcgcgcgcg ccaatggcga ggcacagctc ggcaaatacg gcggcggtgc aggcggggcc gctgcggctt catccctctt cgagaaacgc tacgtgtact aa
Details for TogoA.01236.a.A11.GE33353
HARVESTED ON: 9/12/2011
SEQUENCED ON: 9/20/2011
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL data unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: data unavailable
PERCENT IDENTITY: 97
PERCENT COVERAGE: 70
Validated AA Sequence
MVDLLKAEGI IPGIKVDKXL ETLPLTDDEK ATMGLDGXSE RCKKXYEAGA RFAKWRAVLS IDPAKXKXTN XSITEVAHGL ARYAAICQAN RLVPIVEPEI LTDGSHDITV CAEVTERVLA AVFKALNDHH VLLEGALLKP NMVTHGSDCP KPASHEEIAX YTVRSLKRTV PPALPGVMFL SGGQSEEDAS LNLNEMNKMG PHPFQLSFSY GRALQASCLK AWKGVPENKA KAQQVXMERA RANGEAQLGK YGGGAGGAAA ASSLFEKRYV Y
Validated NT Sequence
ctttgttagc agccggatcc tcgagaagct tggctgcaga acttgttcgt gctgtttatt agtaaacgta gcgcttctca aagaggctgg aagctgcagc tgcaccacct gcaccgccac cgtatttgcc taactgtgct tcaccgttgg cacgagcgcg ttccatcana acttgttgtg ctttagcttt attctcaggc acgcctttcc atgctttcag acagctagcc tgaagggcgc gaccataaga gaagctcagc tggaatggat gaggacccat cttattcatt tcattcagat tcaggctagc atcttcttcg gattgaccgc cggacaggaa cattacaccc ggcagggccg gtggcacagt gcgtttgagg ctacgtacag tgtanaaagc tatttcttca tgcgaagctg gtttcgggca gtcggagcca tgggtcacca tgttaggttt cagcagagcg ccttcaagca gcacgtggtg atcgttcagg gccttaaaca cggcggccag aacacgttcg gtaacctctg cgcaaacggt tatatcgtgg ctaccatcgg tcaggatttc aggctctacg attggcacca gacggttagc ctgacagatg gcagcgtaac gtgccagacc gtgagccacc tcggttatgc tcangttagt cngtttgncc ttagccggat caatggagag aaccgcacgc cacttagcga agcgtgcgcc tgcttcgtag natttcttgc aacgttcgct canaccgtca agacccatcg ttgctttctc atcgtcagtc agcggcaggg tttccaganc tttatcaact ttaatgcctg ggattatacc ttctgctttc aacaggtcaa ccatcnnnng ccngacggag atttttggta cnggtctctt caacnnatag cgnngcnnta tactggnnta annctttant gctgannngn nnnnncgata gaatgcgcnn ttagctnnnt attctccncn cctatgnngt cnaagcgnnn cttgntnnta cngnnnnann nnncanctgc annnnnncnt nnnnnnnnna ncannntgng ngnnnnnnna nnnnntnntt tnncnannnn nnggnnnatn nnnngncnna nnncnnannt cnnnnnnnnn nnnnnnnnnt nnnnnnnnnn nccnnntnna ngnnnnngnn nnnnnnntnn nnnnnnnnnn nnntnnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGYGLPIS QEVAKELAEN ARKIAAPGKG ILAADESTGT IKKRFDSIGV ENTEANRAFY RDLLFSTKGL GQYISGAILF EETLYQKSPS GVPMVDLLKA EGIIPGIKVD KGLETLPLTD DEKATMGLDG LSERCKKYYE AGARFAKWRA VLSIDPAKGK PTNLSITEVA HGLARYAAIC QANRLVPIVE PEILTDGSHD ITVCAEVTER VLAAVFKALN DHHVLLEGAL LKPNMVTHGS DCPKPASHEE IAFYTVRSLK RTVPPALPGV MFLSGGQSEE DASLNLNEMN KMGPHPFQLS FSYGRALQAS CLKAWKGVPE NKAKAQQVLM ERARANGEAQ LGKYGGGAGG AAAASSLFEK RYVY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctggctat ggcctgccga ttagccagga agtagctaaa gaactggctg agaacgcacg caagattgct gctcctggta agggtatcct tgcagctgac gaatccaccg gtacaatcaa gaaacgcttt gactccatag gcgtggagaa taccgaagct aaccgcgcat tctatcgtga ccttctgttc agcactaaag gcttaggcca gtatattagc ggcgctatcc tgtttgaaga gaccctgtac caaaaatctc cgtccggcgt cccgatggtt gacctgttga aagcagaagg tataatccca ggcattaaag ttgataaagg tctggaaacc ctgccgctga ctgacgatga gaaagcaacg atgggtcttg acggtctgag cgaacgttgc aagaaatact acgaagcagg cgcacgcttc gctaagtggc gtgcggttct ctccattgat ccggctaagg gcaaaccgac taacctgagc ataaccgagg tggctcacgg tctggcacgt tacgctgcca tctgtcaggc taaccgtctg gtgccaatcg tagagcctga aatcctgacc gatggtagcc acgatataac cgtttgcgca gaggttaccg aacgtgttct ggccgccgtg tttaaggccc tgaacgatca ccacgtgctg cttgaaggcg ctctgctgaa acctaacatg gtgacccatg gctccgactg cccgaaacca gcttcgcatg aagaaatagc tttctacact gtacgtagcc tcaaacgcac tgtgccaccg gccctgccgg gtgtaatgtt cctgtccggc ggtcaatccg aagaagatgc tagcctgaat ctgaatgaaa tgaataagat gggtcctcat ccattccagc tgagcttctc ttatggtcgc gcccttcagg ctagctgtct gaaagcatgg aaaggcgtgc ctgagaataa agctaaagca caacaagttc tgatggaacg cgctcgtgcc aacggtgaag cacagttagg caaatacggt ggcggtgcag gtggtgcagc tgcagcttcc agcctctttg agaagcgcta cgtttactaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for TogoA.01236.a.A12.GE33354
HARVESTED ON: 9/12/2011
SEQUENCED ON: 9/20/2011
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL data unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: data unavailable
PERCENT IDENTITY: 98
PERCENT COVERAGE: 68
Validated AA Sequence
MSGYGLPISQ EVAKELAENA RKIAAPGKGI LAADESTGTI KKRFDSIGVE NTEANRAFYR DLLFSTKGLG QYISGAILFE ETLYQKSPSG VPMVDLLKAE GIIPGIKVDK GLETLPLTDD EKATMGLDGL SERCKKYYEA GARFAKWRAV LSIDPAKGKP TNLSITEVAH GLARYAAICQ ANRLVPIVEP EILTDGSHDI TVCAEVTERV LAAVFKALND HHVLLEGALL KPNMVTHGSD CPETSFA
Validated NT Sequence
ctggaagctc agacccaggg tcctggttcg atgtctggct atggcctgcc gattagccag gaagtagcta aagaactggc tgagaacgca cgcaagattg ctgctcctgg taagggtatc cttgcagctg acgaatccac cggtacaatc aagaaacgct ttgactccat aggcgtggag aataccgaag ctaaccgcgc attctatcgt gaccttctgt tcagcactaa aggcttaggc cagtatatta gcggcgctat cctgtttgaa gagaccctgt accaaaaatc tccgtccggc gtcccgatgg ttgacctgtt gaaagcagaa ggtataatcc caggcattaa agttgataaa ggtctggaaa ccctgccgct gactgacgat gagaaagcaa cgatgggtct tgacggtctg agcgaacgtt gcaagaaata ctacgaagca ggcgcacgct tcgctaagtg gcgtgcggtt ctctccattg atccggctaa gggcaaaccg actaacctga gcataaccga ggtggctcac ggtctggcac gttacgctgc catctgtcag gctaaccgtc tggtgccaat cgtagagcct gaaatcctga ccgatggtag ccacgatata accgtttgcg cagaggttac cgaacgtgtt ctggccgccg tgtttaaggc cctgaacgat caccacgtgc tgcttgaagg cgctctgctg aaacctaaca tggtgaccca tggctccgac tgccccgaaa ccagcttcgc atgaagaaat agctttctac actgtacgta gcctcaaacg cactgtgcca ccggccctgc cgggtgtaat gttcctgtcc ggcggtcaat ccgaagaaga tgctagcctg aatctgaatg aaatgaataa gatgggtcct catccattcc agctgagctt ctcttatggt cgcgcccttc aggctagctg tctgaaagca tggaaaggcg tgcctgagaa taaagctaaa gcacaacaag ttctgatgga acgcgctcgt gccaacggtg aagca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGYGLPIS QEVAKELAEN ARKIAAPGKG ILAADESTGT IKKRFDSIGV ENTEANRAFY RDLLFSTKGL GQYISGAILF EETLYQKSPS GVPMVDLLKA EGIIPGIKVD KGLETLPLTD DEKATMGLDG LSERCKKYYE AGARFAKWRA VLSIDPAKGK PTNLSITEVA HGLARYAAIC QANRLVPIVE PEILTDGSHD ITVCAEVTER VLAAVFKALN DHHVLLEGAL LKPNMVTHGS DCPKPASHEE IAFYTVRSLK RTVPPALPGV MFLSGGQSEE DASLNLNEMN KMGPHPFQLS FSYGRALQAS CLKAWKGVPE NKAKAQQVLM ERARANGEAQ L
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctggctat ggcctgccga ttagccagga agtagctaaa gaactggctg agaacgcacg caagattgct gctcctggta agggtatcct tgcagctgac gaatccaccg gtacaatcaa gaaacgcttt gactccatag gcgtggagaa taccgaagct aaccgcgcat tctatcgtga ccttctgttc agcactaaag gcttaggcca gtatattagc ggcgctatcc tgtttgaaga gaccctgtac caaaaatctc cgtccggcgt cccgatggtt gacctgttga aagcagaagg tataatccca ggcattaaag ttgataaagg tctggaaacc ctgccgctga ctgacgatga gaaagcaacg atgggtcttg acggtctgag cgaacgttgc aagaaatact acgaagcagg cgcacgcttc gctaagtggc gtgcggttct ctccattgat ccggctaagg gcaaaccgac taacctgagc ataaccgagg tggctcacgg tctggcacgt tacgctgcca tctgtcaggc taaccgtctg gtgccaatcg tagagcctga aatcctgacc gatggtagcc acgatataac cgtttgcgca gaggttaccg aacgtgttct ggccgccgtg tttaaggccc tgaacgatca ccacgtgctg cttgaaggcg ctctgctgaa acctaacatg gtgacccatg gctccgactg cccgaaacca gcttcgcatg aagaaatagc tttctacact gtacgtagcc tcaaacgcac tgtgccaccg gccctgccgg gtgtaatgtt cctgtccggc ggtcaatccg aagaagatgc tagcctgaat ctgaatgaaa tgaataagat gggtcctcat ccattccagc tgagcttctc ttatggtcgc gcccttcagg ctagctgtct gaaagcatgg aaaggcgtgc ctgagaataa agctaaagca caacaagttc tgatggaacg cgctcgtgcc aacggtgaag cacagttata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for TogoA.01236.a.A13.GE33355
HARVESTED ON: 9/12/2011
SEQUENCED ON: 9/20/2011
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL data unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: data unavailable
PERCENT IDENTITY: 98
PERCENT COVERAGE: 89
Validated AA Sequence
MSGYGLPISQ EVAKELAENA RKIAAPGKGI LAADESTGTI KKRFDSIGVE NTEANRAFYR DLLFSTKGLG QYISGAILFE ETLYQKSPSG VPMVDLLKAE GIIPGIKVDK GLETLPLTDD EKATMGLDGL SERCKKYYEA GARFAKWRAV LSIDPAKGKP TNLSITEVAH GLARYAAICQ ANRLVPIVEP EILTDGSHDI TVCAEVTERV LAAVFKALND HHVLLEGALL KPNMVTHGSD CPKPASHEEI AFYTVRSLKR TVPPALPGVM FLSGGQSEED ASLNLNEMNK XGPHPFXLSF SYGRALQASC XXAWKGVPEN KAKAQQVXME R
Validated NT Sequence
ccctggaagc tcagacccag ggtcctggtt cgatgtctgg ctatggcctg ccgattagcc aggaagtagc taaagaactg gctgagaacg cacgcaagat tgctgctcct ggtaagggta tccttgcagc tgacgaatcc accggtacaa tcaagaaacg ctttgactcc ataggcgtgg agaataccga agctaaccgc gcattctatc gtgaccttct gttcagcact aaaggcttag gccagtatat tagcggcgct atcctgtttg aagagaccct gtaccaaaaa tctccgtccg gcgtcccgat ggttgacctg ttgaaagcag aaggtataat cccaggcatt aaagttgata aaggtctgga aaccctgccg ctgactgacg atgagaaagc aacgatgggt cttgacggtc tgagcgaacg ttgcaagaaa tactacgaag caggcgcacg cttcgctaag tggcgtgcgg ttctctccat tgatccggct aagggcaaac cgactaacct gagcataacc gaggtggctc acggtctggc acgttacgct gccatctgtc aggctaaccg tctggtgcca atcgtagagc ctgaaatcct gaccgatggt agccacgata taaccgtttg cgcagaggtt accgaacgtg ttctggccgc cgtgtttaag gccctgaacg atcaccacgt gctgcttgaa ggcgctctgc tgaaacctaa catggtgacc catggctccg actgcccgaa accagcttcg catgaagaaa tagctttcta cactgtacgt agcctcaaac gcactgtgcc accggccctg ccgggtgtaa tgttcctgtc cggcggtcaa tccgaagaag atgctagcct gaatctgaat gaaatgaata agannggtcc tcatccattc canctgagct tctcttatgg tcgcgccctt caggctagct gtnnnanagc atggaaaggc gtgcctgaga ataaagctaa agcacaacaa gttnngatgg aacgcgc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGYGLPIS QEVAKELAEN ARKIAAPGKG ILAADESTGT IKKRFDSIGV ENTEANRAFY RDLLFSTKGL GQYISGAILF EETLYQKSPS GVPMVDLLKA EGIIPGIKVD KGLETLPLTD DEKATMGLDG LSERCKKYYE AGARFAKWRA VLSIDPAKGK PTNLSITEVA HGLARYAAIC QANRLVPIVE PEILTDGSHD ITVCAEVTER VLAAVFKALN DHHVLLEGAL LKPNMVTHGS DCPKPASHEE IAFYTVRSLK RTVPPALPGV MFLSGGQSEE DASLNLNEMN KMGPHPFQLS FSYGRALQAS CLKAWKGVPE NKAKAQQVLM ERARANGEAQ LGKYGGGAGG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctggctat ggcctgccga ttagccagga agtagctaaa gaactggctg agaacgcacg caagattgct gctcctggta agggtatcct tgcagctgac gaatccaccg gtacaatcaa gaaacgcttt gactccatag gcgtggagaa taccgaagct aaccgcgcat tctatcgtga ccttctgttc agcactaaag gcttaggcca gtatattagc ggcgctatcc tgtttgaaga gaccctgtac caaaaatctc cgtccggcgt cccgatggtt gacctgttga aagcagaagg tataatccca ggcattaaag ttgataaagg tctggaaacc ctgccgctga ctgacgatga gaaagcaacg atgggtcttg acggtctgag cgaacgttgc aagaaatact acgaagcagg cgcacgcttc gctaagtggc gtgcggttct ctccattgat ccggctaagg gcaaaccgac taacctgagc ataaccgagg tggctcacgg tctggcacgt tacgctgcca tctgtcaggc taaccgtctg gtgccaatcg tagagcctga aatcctgacc gatggtagcc acgatataac cgtttgcgca gaggttaccg aacgtgttct ggccgccgtg tttaaggccc tgaacgatca ccacgtgctg cttgaaggcg ctctgctgaa acctaacatg gtgacccatg gctccgactg cccgaaacca gcttcgcatg aagaaatagc tttctacact gtacgtagcc tcaaacgcac tgtgccaccg gccctgccgg gtgtaatgtt cctgtccggc ggtcaatccg aagaagatgc tagcctgaat ctgaatgaaa tgaataagat gggtcctcat ccattccagc tgagcttctc ttatggtcgc gcccttcagg ctagctgtct gaaagcatgg aaaggcgtgc ctgagaataa agctaaagca caacaagttc tgatggaacg cgctcgtgcc aacggtgaag cacagttagg caaatacggt ggcggtgcag gtggttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for TogoA.01236.a.A15.GE33357
HARVESTED ON: 9/12/2011
SEQUENCED ON: 9/20/2011
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL data unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMISQEVAKE LAENARKIAA PGKGILAADE STGTIKKRFD SIGVENTEAN RAFYRDLLFS TKGLGQYISG AILFEETLYQ KSPSGVPMVD LLKAEGIIPG IKVDKGLETL PLTDDEKATM GLDGLSERCK KYYEAGARFA KWRAVLSIDP AKGKPTNLSI TEVAHGLARY AAICQANRLV PIVEPEILTD GSHDITVCAE VTERVLAAVF KALNDHHVLL EGALLKPNMV THGSDCPKPA SHEEIAFYTV RSLKRTVPPA LPGVMFLSGG QSEEDASLNL NEMNKMGPHP FQLSFSYGRA LQASCLKAWK GVPENKAKAQ QVLMERARAN GE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatta gccaggaagt agctaaagaa ctggctgaga acgcacgcaa gattgctgct cctggtaagg gtatccttgc agctgacgaa tccaccggta caatcaagaa acgctttgac tccataggcg tggagaatac cgaagctaac cgcgcattct atcgtgacct tctgttcagc actaaaggct taggccagta tattagcggc gctatcctgt ttgaagagac cctgtaccaa aaatctccgt ccggcgtccc gatggttgac ctgttgaaag cagaaggtat aatcccaggc attaaagttg ataaaggtct ggaaaccctg ccgctgactg acgatgagaa agcaacgatg ggtcttgacg gtctgagcga acgttgcaag aaatactacg aagcaggcgc acgcttcgct aagtggcgtg cggttctctc cattgatccg gctaagggca aaccgactaa cctgagcata accgaggtgg ctcacggtct ggcacgttac gctgccatct gtcaggctaa ccgtctggtg ccaatcgtag agcctgaaat cctgaccgat ggtagccacg atataaccgt ttgcgcagag gttaccgaac gtgttctggc cgccgtgttt aaggccctga acgatcacca cgtgctgctt gaaggcgctc tgctgaaacc taacatggtg acccatggct ccgactgccc gaaaccagct tcgcatgaag aaatagcttt ctacactgta cgtagcctca aacgcactgt gccaccggcc ctgccgggtg taatgttcct gtccggcggt caatccgaag aagatgctag cctgaatctg aatgaaatga ataagatggg tcctcatcca ttccagctga gcttctctta tggtcgcgcc cttcaggcta gctgtctgaa agcatggaaa ggcgtgcctg agaataaagc taaagcacaa caagttctga tggaacgcgc tcgtgccaac ggtgaagc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMISQEVAKE LAENARKIAA PGKGILAADE STGTIKKRFD SIGVENTEAN RAFYRDLLFS TKGLGQYISG AILFEETLYQ KSPSGVPMVD LLKAEGIIPG IKVDKGLETL PLTDDEKATM GLDGLSERCK KYYEAGARFA KWRAVLSIDP AKGKPTNLSI TEVAHGLARY AAICQANRLV PIVEPEILTD GSHDITVCAE VTERVLAAVF KALNDHHVLL EGALLKPNMV THGSDCPKPA SHEEIAFYTV RSLKRTVPPA LPGVMFLSGG QSEEDASLNL NEMNKMGPHP FQLSFSYGRA LQASCLKAWK GVPENKAKAQ QVLMERARAN GEAQL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattagccag gaagtagcta aagaactggc tgagaacgca cgcaagattg ctgctcctgg taagggtatc cttgcagctg acgaatccac cggtacaatc aagaaacgct ttgactccat aggcgtggag aataccgaag ctaaccgcgc attctatcgt gaccttctgt tcagcactaa aggcttaggc cagtatatta gcggcgctat cctgtttgaa gagaccctgt accaaaaatc tccgtccggc gtcccgatgg ttgacctgtt gaaagcagaa ggtataatcc caggcattaa agttgataaa ggtctggaaa ccctgccgct gactgacgat gagaaagcaa cgatgggtct tgacggtctg agcgaacgtt gcaagaaata ctacgaagca ggcgcacgct tcgctaagtg gcgtgcggtt ctctccattg atccggctaa gggcaaaccg actaacctga gcataaccga ggtggctcac ggtctggcac gttacgctgc catctgtcag gctaaccgtc tggtgccaat cgtagagcct gaaatcctga ccgatggtag ccacgatata accgtttgcg cagaggttac cgaacgtgtt ctggccgccg tgtttaaggc cctgaacgat caccacgtgc tgcttgaagg cgctctgctg aaacctaaca tggtgaccca tggctccgac tgcccgaaac cagcttcgca tgaagaaata gctttctaca ctgtacgtag cctcaaacgc actgtgccac cggccctgcc gggtgtaatg ttcctgtccg gcggtcaatc cgaagaagat gctagcctga atctgaatga aatgaataag atgggtcctc atccattcca gctgagcttc tcttatggtc gcgcccttca ggctagctgt ctgaaagcat ggaaaggcgt gcctgagaat aaagctaaag cacaacaagt tctgatggaa cgcgctcgtg ccaacggtga agcacagtta taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for TogoA.01236.a.A16.GE33358
HARVESTED ON: 9/12/2011
SEQUENCED ON: 9/20/2011
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL data unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: data unavailable
PERCENT IDENTITY: 99
PERCENT COVERAGE: 94
Validated AA Sequence
MISQEVAKEL AENARKIAAP GKGILAADES TGTIKKRFDS IGVENTEANR AFYRDLLFST KGLGQYISGA ILFEETLYQK SPSGVPMVDL LKAEGIIPGI KVDKGLETLP LTDDEKATMG LDGLSERCKK YYEAGARFAK WRAVLSIDPA KGKPTNLSIT EVAHGLARYA AICQANRLVP IVEPEILTDG SHDITVCAEV TERVLAAVFK ALNDHHVLLE GALLKPNMVT HGSDCPKPAS HEEIAFYTVR SLKRTVPPAL PGVMFLSGGQ SEEDASLNLN EMNKMGPHPF QLSFSYGRAL QASCLKAWKG VPENKAKAQQ VLMERARANG EAQLGKXGGG AGG
Validated NT Sequence
agctcagacc cagggtcctg gttcgatgat tagccaggaa gtagctaaag aactggctga gaacgcacgc aagattgctg ctcctggtaa gggtatcctt gcagctgacg aatccaccgg tacaatcaag aaacgctttg actccatagg cgtggagaat accgaagcta accgcgcatt ctatcgtgac cttctgttca gcactaaagg cttaggccag tatattagcg gcgctatcct gtttgaagag accctgtacc aaaaatctcc gtccggcgtc ccgatggttg acctgttgaa agcagaaggt ataatcccag gcattaaagt tgataaaggt ctggaaaccc tgccgctgac tgacgatgag aaagcaacga tgggtcttga cggtctgagc gaacgttgca agaaatacta cgaagcaggc gcacgcttcg ctaagtggcg tgcggttctc tccattgatc cggctaaggg caaaccgact aacctgagca taaccgaggt ggctcacggt ctggcacgtt acgctgccat ctgtcaggct aaccgtctgg tgccaatcgt agagcctgaa atcctgaccg atggtagcca cgatataacc gtttgcgcag aggttaccga acgtgttctg gccgccgtgt ttaaggccct gaacgatcac cacgtgctgc ttgaaggcgc tctgctgaaa cctaacatgg tgacccatgg ctccgactgc ccgaaaccag cttcgcatga agaaatagct ttctacactg tacgtagcct caaacgcact gtgccaccgg ccctgccggg ngtaatgttc ctgtccggcg gtcaatccga agaagatgct agcctgaatc tgaatgaaat gaataagatg ggtcctcatc cattccagct gagcttctct tatggtcgcg cccttcaggc tagctgtctg aaagcatgga aaggcgtgcc tgagaataaa gctaaagcac aacaagttct gatggaacgc gctcgtgcca acggtgaagc acagttaggc aaatncggtg gcggtgcagg tggttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMISQEVAKE LAENARKIAA PGKGILAADE STGTIKKRFD SIGVENTEAN RAFYRDLLFS TKGLGQYISG AILFEETLYQ KSPSGVPMVD LLKAEGIIPG IKVDKGLETL PLTDDEKATM GLDGLSERCK KYYEAGARFA KWRAVLSIDP AKGKPTNLSI TEVAHGLARY AAICQANRLV PIVEPEILTD GSHDITVCAE VTERVLAAVF KALNDHHVLL EGALLKPNMV THGSDCPKPA SHEEIAFYTV RSLKRTVPPA LPGVMFLSGG QSEEDASLNL NEMNKMGPHP FQLSFSYGRA LQASCLKAWK GVPENKAKAQ QVLMERARAN GEAQLGKYGG GAGG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattagccag gaagtagcta aagaactggc tgagaacgca cgcaagattg ctgctcctgg taagggtatc cttgcagctg acgaatccac cggtacaatc aagaaacgct ttgactccat aggcgtggag aataccgaag ctaaccgcgc attctatcgt gaccttctgt tcagcactaa aggcttaggc cagtatatta gcggcgctat cctgtttgaa gagaccctgt accaaaaatc tccgtccggc gtcccgatgg ttgacctgtt gaaagcagaa ggtataatcc caggcattaa agttgataaa ggtctggaaa ccctgccgct gactgacgat gagaaagcaa cgatgggtct tgacggtctg agcgaacgtt gcaagaaata ctacgaagca ggcgcacgct tcgctaagtg gcgtgcggtt ctctccattg atccggctaa gggcaaaccg actaacctga gcataaccga ggtggctcac ggtctggcac gttacgctgc catctgtcag gctaaccgtc tggtgccaat cgtagagcct gaaatcctga ccgatggtag ccacgatata accgtttgcg cagaggttac cgaacgtgtt ctggccgccg tgtttaaggc cctgaacgat caccacgtgc tgcttgaagg cgctctgctg aaacctaaca tggtgaccca tggctccgac tgcccgaaac cagcttcgca tgaagaaata gctttctaca ctgtacgtag cctcaaacgc actgtgccac cggccctgcc gggtgtaatg ttcctgtccg gcggtcaatc cgaagaagat gctagcctga atctgaatga aatgaataag atgggtcctc atccattcca gctgagcttc tcttatggtc gcgcccttca ggctagctgt ctgaaagcat ggaaaggcgt gcctgagaat aaagctaaag cacaacaagt tctgatggaa cgcgctcgtg ccaacggtga agcacagtta ggcaaatacg gtggcggtgc aggtggttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for TogoA.01236.a.A13.PS01397
PURIFICATION DATe: 2/27/2012
CONCENTRATION: 60.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 89
Protocol Notes
notes unavailable
Validated AA Sequence
MSGYGLPISQ EVAKELAENA RKIAAPGKGI LAADESTGTI KKRFDSIGVE NTEANRAFYR DLLFSTKGLG QYISGAILFE ETLYQKSPSG VPMVDLLKAE GIIPGIKVDK GLETLPLTDD EKATMGLDGL SERCKKYYEA GARFAKWRAV LSIDPAKGKP TNLSITEVAH GLARYAAICQ ANRLVPIVEP EILTDGSHDI TVCAEVTERV LAAVFKALND HHVLLEGALL KPNMVTHGSD CPKPASHEEI AFYTVRSLKR TVPPALPGVM FLSGGQSEED ASLNLNEMNK XGPHPFXLSF SYGRALQASC XXAWKGVPEN KAKAQQVXME R
Validated NT Sequence
ccctggaagc tcagacccag ggtcctggtt cgatgtctgg ctatggcctg ccgattagcc aggaagtagc taaagaactg gctgagaacg cacgcaagat tgctgctcct ggtaagggta tccttgcagc tgacgaatcc accggtacaa tcaagaaacg ctttgactcc ataggcgtgg agaataccga agctaaccgc gcattctatc gtgaccttct gttcagcact aaaggcttag gccagtatat tagcggcgct atcctgtttg aagagaccct gtaccaaaaa tctccgtccg gcgtcccgat ggttgacctg ttgaaagcag aaggtataat cccaggcatt aaagttgata aaggtctgga aaccctgccg ctgactgacg atgagaaagc aacgatgggt cttgacggtc tgagcgaacg ttgcaagaaa tactacgaag caggcgcacg cttcgctaag tggcgtgcgg ttctctccat tgatccggct aagggcaaac cgactaacct gagcataacc gaggtggctc acggtctggc acgttacgct gccatctgtc aggctaaccg tctggtgcca atcgtagagc ctgaaatcct gaccgatggt agccacgata taaccgtttg cgcagaggtt accgaacgtg ttctggccgc cgtgtttaag gccctgaacg atcaccacgt gctgcttgaa ggcgctctgc tgaaacctaa catggtgacc catggctccg actgcccgaa accagcttcg catgaagaaa tagctttcta cactgtacgt agcctcaaac gcactgtgcc accggccctg ccgggtgtaa tgttcctgtc cggcggtcaa tccgaagaag atgctagcct gaatctgaat gaaatgaata agannggtcc tcatccattc canctgagct tctcttatgg tcgcgccctt caggctagct gtnnnanagc atggaaaggc gtgcctgaga ataaagctaa agcacaacaa gttnngatgg aacgcgc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGYGLPIS QEVAKELAEN ARKIAAPGKG ILAADESTGT IKKRFDSIGV ENTEANRAFY RDLLFSTKGL GQYISGAILF EETLYQKSPS GVPMVDLLKA EGIIPGIKVD KGLETLPLTD DEKATMGLDG LSERCKKYYE AGARFAKWRA VLSIDPAKGK PTNLSITEVA HGLARYAAIC QANRLVPIVE PEILTDGSHD ITVCAEVTER VLAAVFKALN DHHVLLEGAL LKPNMVTHGS DCPKPASHEE IAFYTVRSLK RTVPPALPGV MFLSGGQSEE DASLNLNEMN KMGPHPFQLS FSYGRALQAS CLKAWKGVPE NKAKAQQVLM ERARANGEAQ LGKYGGGAGG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTGGCTAT GGCCTGCCGA TTAGCCAGGA AGTAGCTAAA GAACTGGCTG AGAACGCACG CAAGATTGCT GCTCCTGGTA AGGGTATCCT TGCAGCTGAC GAATCCACCG GTACAATCAA GAAACGCTTT GACTCCATAG GCGTGGAGAA TACCGAAGCT AACCGCGCAT TCTATCGTGA CCTTCTGTTC AGCACTAAAG GCTTAGGCCA GTATATTAGC GGCGCTATCC TGTTTGAAGA GACCCTGTAC CAAAAATCTC CGTCCGGCGT CCCGATGGTT GACCTGTTGA AAGCAGAAGG TATAATCCCA GGCATTAAAG TTGATAAAGG TCTGGAAACC CTGCCGCTGA CTGACGATGA GAAAGCAACG ATGGGTCTTG ACGGTCTGAG CGAACGTTGC AAGAAATACT ACGAAGCAGG CGCACGCTTC GCTAAGTGGC GTGCGGTTCT CTCCATTGAT CCGGCTAAGG GCAAACCGAC TAACCTGAGC ATAACCGAGG TGGCTCACGG TCTGGCACGT TACGCTGCCA TCTGTCAGGC TAACCGTCTG GTGCCAATCG TAGAGCCTGA AATCCTGACC GATGGTAGCC ACGATATAAC CGTTTGCGCA GAGGTTACCG AACGTGTTCT GGCCGCCGTG TTTAAGGCCC TGAACGATCA CCACGTGCTG CTTGAAGGCG CTCTGCTGAA ACCTAACATG GTGACCCATG GCTCCGACTG CCCGAAACCA GCTTCGCATG AAGAAATAGC TTTCTACACT GTACGTAGCC TCAAACGCAC TGTGCCACCG GCCCTGCCGG GTGTAATGTT CCTGTCCGGC GGTCAATCCG AAGAAGATGC TAGCCTGAAT CTGAATGAAA TGAATAAGAT GGGTCCTCAT CCATTCCAGC TGAGCTTCTC TTATGGTCGC GCCCTTCAGG CTAGCTGTCT GAAAGCATGG AAAGGCGTGC CTGAGAATAA AGCTAAAGCA CAACAAGTTC TGATGGAACG CGCTCGTGCC AACGGTGAAG CACAGTTAGG CAAATACGGT GGCGGTGCAG GTGGTTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for TogoA.01236.a.A15.PW36055
PURIFICATION DATe: 1/13/2012
CONCENTRATION: 26.65mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMISQEVAKE LAENARKIAA PGKGILAADE STGTIKKRFD SIGVENTEAN RAFYRDLLFS TKGLGQYISG AILFEETLYQ KSPSGVPMVD LLKAEGIIPG IKVDKGLETL PLTDDEKATM GLDGLSERCK KYYEAGARFA KWRAVLSIDP AKGKPTNLSI TEVAHGLARY AAICQANRLV PIVEPEILTD GSHDITVCAE VTERVLAAVF KALNDHHVLL EGALLKPNMV THGSDCPKPA SHEEIAFYTV RSLKRTVPPA LPGVMFLSGG QSEEDASLNL NEMNKMGPHP FQLSFSYGRA LQASCLKAWK GVPENKAKAQ QVLMERARAN GE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatta gccaggaagt agctaaagaa ctggctgaga acgcacgcaa gattgctgct cctggtaagg gtatccttgc agctgacgaa tccaccggta caatcaagaa acgctttgac tccataggcg tggagaatac cgaagctaac cgcgcattct atcgtgacct tctgttcagc actaaaggct taggccagta tattagcggc gctatcctgt ttgaagagac cctgtaccaa aaatctccgt ccggcgtccc gatggttgac ctgttgaaag cagaaggtat aatcccaggc attaaagttg ataaaggtct ggaaaccctg ccgctgactg acgatgagaa agcaacgatg ggtcttgacg gtctgagcga acgttgcaag aaatactacg aagcaggcgc acgcttcgct aagtggcgtg cggttctctc cattgatccg gctaagggca aaccgactaa cctgagcata accgaggtgg ctcacggtct ggcacgttac gctgccatct gtcaggctaa ccgtctggtg ccaatcgtag agcctgaaat cctgaccgat ggtagccacg atataaccgt ttgcgcagag gttaccgaac gtgttctggc cgccgtgttt aaggccctga acgatcacca cgtgctgctt gaaggcgctc tgctgaaacc taacatggtg acccatggct ccgactgccc gaaaccagct tcgcatgaag aaatagcttt ctacactgta cgtagcctca aacgcactgt gccaccggcc ctgccgggtg taatgttcct gtccggcggt caatccgaag aagatgctag cctgaatctg aatgaaatga ataagatggg tcctcatcca ttccagctga gcttctctta tggtcgcgcc cttcaggcta gctgtctgaa agcatggaaa ggcgtgcctg agaataaagc taaagcacaa caagttctga tggaacgcgc tcgtgccaac ggtgaagc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMISQEVAKE LAENARKIAA PGKGILAADE STGTIKKRFD SIGVENTEAN RAFYRDLLFS TKGLGQYISG AILFEETLYQ KSPSGVPMVD LLKAEGIIPG IKVDKGLETL PLTDDEKATM GLDGLSERCK KYYEAGARFA KWRAVLSIDP AKGKPTNLSI TEVAHGLARY AAICQANRLV PIVEPEILTD GSHDITVCAE VTERVLAAVF KALNDHHVLL EGALLKPNMV THGSDCPKPA SHEEIAFYTV RSLKRTVPPA LPGVMFLSGG QSEEDASLNL NEMNKMGPHP FQLSFSYGRA LQASCLKAWK GVPENKAKAQ QVLMERARAN GEAQL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATTAGCCAG GAAGTAGCTA AAGAACTGGC TGAGAACGCA CGCAAGATTG CTGCTCCTGG TAAGGGTATC CTTGCAGCTG ACGAATCCAC CGGTACAATC AAGAAACGCT TTGACTCCAT AGGCGTGGAG AATACCGAAG CTAACCGCGC ATTCTATCGT GACCTTCTGT TCAGCACTAA AGGCTTAGGC CAGTATATTA GCGGCGCTAT CCTGTTTGAA GAGACCCTGT ACCAAAAATC TCCGTCCGGC GTCCCGATGG TTGACCTGTT GAAAGCAGAA GGTATAATCC CAGGCATTAA AGTTGATAAA GGTCTGGAAA CCCTGCCGCT GACTGACGAT GAGAAAGCAA CGATGGGTCT TGACGGTCTG AGCGAACGTT GCAAGAAATA CTACGAAGCA GGCGCACGCT TCGCTAAGTG GCGTGCGGTT CTCTCCATTG ATCCGGCTAA GGGCAAACCG ACTAACCTGA GCATAACCGA GGTGGCTCAC GGTCTGGCAC GTTACGCTGC CATCTGTCAG GCTAACCGTC TGGTGCCAAT CGTAGAGCCT GAAATCCTGA CCGATGGTAG CCACGATATA ACCGTTTGCG CAGAGGTTAC CGAACGTGTT CTGGCCGCCG TGTTTAAGGC CCTGAACGAT CACCACGTGC TGCTTGAAGG CGCTCTGCTG AAACCTAACA TGGTGACCCA TGGCTCCGAC TGCCCGAAAC CAGCTTCGCA TGAAGAAATA GCTTTCTACA CTGTACGTAG CCTCAAACGC ACTGTGCCAC CGGCCCTGCC GGGTGTAATG TTCCTGTCCG GCGGTCAATC CGAAGAAGAT GCTAGCCTGA ATCTGAATGA AATGAATAAG ATGGGTCCTC ATCCATTCCA GCTGAGCTTC TCTTATGGTC GCGCCCTTCA GGCTAGCTGT CTGAAAGCAT GGAAAGGCGT GCCTGAGAAT AAAGCTAAAG CACAACAAGT TCTGATGGAA CGCGCTCGTG CCAACGGTGA AGCACAGTTA TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA