CENTER ID: | TogoA.01236.a |
ORGANISM: | Toxoplasma gondii ME49 |
ASSOCIATED DISEASE: | Toxoplasmosis |
CURRENT STATUS: | in PDB |
COMMUNITY REQUEST: | True |
NIH RISK GROUP: | 2 |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
IIIB |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
TogoA.01236.a.A11.GE33353 | full length | 71 | 433 | ||
TogoA.01236.a.A12.GE33354 | full length | 71 | 410 | ||
TogoA.01236.a.A13.GE33355 | full length | 71 | 419 | ||
TogoA.01236.a.A15.GE33357 | full length | 78 | 410 | ||
TogoA.01236.a.A16.GE33358 | full length | 78 | 419 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
TogoA.01236.a.A13.PS01397 | full length | 71 | 419 | ||
TogoA.01236.a.A15.PW36055 | full length | 78 | 410 |
RESOURCE | REFERENCE ID |
---|---|
EuPathDB: | ToxoDB:TGME49_236040 |
OrthoMCL: | OG5_127143 |
RefSeq: | XP_002368989.1 |
UniProt: | B9PW35 |
HARVESTED ON: | 9/12/2011 |
SEQUENCED ON: | 9/20/2011 |
EXPECTED MW: | 41kDa |
OBSERVED MW: | 41kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | data unavailable |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | data unavailable |
PERCENT IDENTITY: | 97 |
PERCENT COVERAGE: | 70 |
HARVESTED ON: | 9/12/2011 |
SEQUENCED ON: | 9/20/2011 |
EXPECTED MW: | 39kDa |
OBSERVED MW: | 39kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | data unavailable |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | data unavailable |
PERCENT IDENTITY: | 98 |
PERCENT COVERAGE: | 68 |
HARVESTED ON: | 9/12/2011 |
SEQUENCED ON: | 9/20/2011 |
EXPECTED MW: | 39kDa |
OBSERVED MW: | 39kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | data unavailable |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | data unavailable |
PERCENT IDENTITY: | 98 |
PERCENT COVERAGE: | 89 |
HARVESTED ON: | 9/12/2011 |
SEQUENCED ON: | 9/20/2011 |
EXPECTED MW: | 38kDa |
OBSERVED MW: | 38kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | data unavailable |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | data unavailable |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 99 |
HARVESTED ON: | 9/12/2011 |
SEQUENCED ON: | 9/20/2011 |
EXPECTED MW: | 39kDa |
OBSERVED MW: | 39kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | data unavailable |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | data unavailable |
PERCENT IDENTITY: | 99 |
PERCENT COVERAGE: | 94 |
PURIFICATION DATe: | 2/27/2012 |
CONCENTRATION: | 60.7mg/ml |
OBSERVED MW: | data unavailable |
EXPRESSION LEVEL: | Low Expression |
PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
EXPRESSION HOST: | data unavailable |
VIAL COUNT (approx.): | 6 |
VIAL VOLUME: | 200µl |
PERCENT IDENTITY: | 98 |
PERCENT COVERAGE: | 89 |
PURIFICATION DATe: | 1/13/2012 |
CONCENTRATION: | 26.65mg/ml |
OBSERVED MW: | 38kDa |
EXPRESSION LEVEL: | High Expression |
PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
EXPRESSION HOST: | data unavailable |
VIAL COUNT (approx.): | 4 |
VIAL VOLUME: | 110µl |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 99 |