TogoA.00914.a
Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)
| CENTER ID: | TogoA.00914.a |
| ORGANISM: | Toxoplasma gondii ME49 |
| ASSOCIATED DISEASE: | Toxoplasmosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TogoA.00914.a.A1.GE28112 | full length | 1 | 340 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TogoA.00914.a.A1.PS01526 | full length | 1 | 340 |
Structures
3STH
DEPOSITED: 7/10/2011
DETERMINATION: XRay
CLONE: TogoA.00914.a.A1.GE28112
PROTEIN: TogoA.00914.a.A1.PW28008
Publications by SSGCID
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | ToxoDB:TGME49_289690 |
| OrthoMCL: | OG5_126628 |
| RefSeq: | XP_002368394.1 |
| UniProt: | Q9BKE2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVCKLGINGF GRIGRLVFRA AMERGDVEVL AINDPFMSLD YMVYLLRYDS VHGHYPGEVS HKDGKLIVGG KAVTVFNEKE PTAIPWGQAG VHYICESTGI FLTKEKAQAH LTNGAKKVIM SAPPKDDTPM FVMGVNNDQY KSSDVIVSNA SCTTNCLAPL AKIVHDKFGI VEGLMTTVHA MTANQLTVDG PSKGGKDWRA GRSAGVNIIP ASTGAAKAVG KIIPSLNGKL TGMAFRVPVP DVSVVDLTCK LAKPAKYEDI VAAVKEAATS GPMKGIISYT DEEVVSSDFV HCKFSSVFDI NAGIMLNDTF VKLVSWYDNE WGYSNRLVEL AHYMSVQDGA
NT Sequence
atggtgtgca agctgggtat taacggtttt ggccgtattg gccgtctggt gttccgtgct gcgatggaac gcggcgacgt ggaggttttg gcgatcaacg acccgttcat gtccctggac tacatggtgt accttctgag atacgactcg gtccacggcc actaccccgg cgaagtcagc cacaaggacg gcaagctcat tgtcggcgga aaggccgtga ctgtcttcaa cgagaaggag ccaactgcca tcccttgggg ccaggctggc gtccactaca tctgcgagtc caccggtatt ttcctcacca aagagaaggc tcaggctcac ttgaccaacg gcgcgaagaa ggtcatcatg tccgcccccc ccaaggacga caccccgatg ttcgtcatgg gtgtcaacaa tgaccagtac aagtccagcg atgtcattgt gtcgaacgcc tcttgcacga ccaactgctt ggctcctctc gccaaaattg tccacgataa gttcggcatc gttgagggtc tcatgaccac cgtccatgcg atgacggcga accagctgac ggttgatgga ccctccaagg gtggcaaaga ttggcgcgct ggccggtcgg caggcgtcaa catcatccct gcctccactg gtgccgccaa ggctgtgggc aagattattc cttccctgaa cggcaagctg accggtatgg ctttccgtgt gcccgtcccc gacgtgtctg tggttgatct gacctgcaag ctggccaagc ccgcgaagta cgaggacatc gtggcagctg tgaaggaggc tgccacctcc ggccccatga agggaatcat cagctacacc gacgaagagg tcgtctcctc tgacttcgtc cactgcaagt tctcctccgt gtttgacatc aacgccggca tcatgcttaa cgacaccttc gttaagctcg tgtcttggta cgacaacgag tggggatact ccaaccgcct tgttgagctg gctcactaca tgtccgtcca ggacggcgcg taa
Details for TogoA.00914.a.A1.GE28112
| HARVESTED ON: | 12/22/2009 |
| SEQUENCED ON: | 12/31/2009 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVCKLGING FGRIGRLVFR AAMERGDVEV LAINDPFMSL DYMVYLLRYD SVHGHYPGEV SHKDGKLIVG GKAVTVFNEK EPTAIPWGQA GVHYICESTG IFLTKEKAQA HLTNGAKKVI MSAPPKDDTP MFVMGVNNDQ YKSSDVIVSN ASCTTNCLAP LAKIVHDKFG IVEGLMTTVH AMTANQLTVD GPSKGGKDWR AGRSAGVNII PASTGAAKAV GKIIPSLNGK LTGMAFRVPV PDVSVVDLTC KLAKPAKYED IVAAVKEAAT SGPMKGIISY TDEEVVSSDF VHCKFSSVFD INAGIMLNDT FVKLVSWYDN EWGYSNRLVE LAHYMSVQDG A
Validated NT Sequence
ttattacgcg ccgtcctgga cggacatgta gtgagccagc tcaacaaggc ggttggagta tccccactcg ttgtcgtacc aagacacgag cttaacgaag gtgtcgttaa gcatgatgcc ggcgttgatg tcaaacacgg aggagaactt gcagtggacg aagtcagagg agacgacctc ttcgtcggtg tagctgatga ttcccttcat ggggccggag gtggcagcct ccttcacagc tgccacgatg tcctcgtact tcgcgggctt ggccagcttg caggtcagat caaccacaga cacgtcgggg acgggcacac ggaaagccat accggtcagc ttgccgttca gggaaggaat aatcttgccc acagccttgg cggcaccagt ggaggcaggg atgatgttga cgcctgccga ccggccagcg cgccaatctt tgccaccctt ggagggtcca tcaaccgtca gctggttcgc cgtcatcgca tggacggtgg tcatgagacc ctcaacgatg ccgaacttat cgtggacaat tttggcgaga ggagccaagc agttggtcgt gcaagaggcg ttcgacacaa tgacatcgct ggacttgtac tggtcattgt tgacacccat gacgaacatc ggggtgtcgt ccttgggggg ggcggacatg atgaccttct tcgcgccgtt ggtcaagtga gcctgagcct tctctttggt aaggaaaata ccggtggact cgcagatgta gtggacgcca gcctggcccc aagggatggc agttggctcc ttctcgttga agacagtcac ggcctttccg ccgacaatga gcttgccgtc cttgtggctg acttcgccgg ggtagtggcc gtggaccgag tcgtatctca gaaggtacac catgtagtcc agggacatga acgggtcgtt gatcgccaaa acctccacgt cgccgcgttc catcgcagca cggaacacca gacggccaat acggccaaaa ccgttaatac ccagcttgca caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtatatctcc tt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVCKLGING FGRIGRLVFR AAMERGDVEV LAINDPFMSL DYMVYLLRYD SVHGHYPGEV SHKDGKLIVG GKAVTVFNEK EPTAIPWGQA GVHYICESTG IFLTKEKAQA HLTNGAKKVI MSAPPKDDTP MFVMGVNNDQ YKSSDVIVSN ASCTTNCLAP LAKIVHDKFG IVEGLMTTVH AMTANQLTVD GPSKGGKDWR AGRSAGVNII PASTGAAKAV GKIIPSLNGK LTGMAFRVPV PDVSVVDLTC KLAKPAKYED IVAAVKEAAT SGPMKGIISY TDEEVVSSDF VHCKFSSVFD INAGIMLNDT FVKLVSWYDN EWGYSNRLVE LAHYMSVQDG A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtgcaag ctgggtatta acggttttgg ccgtattggc cgtctggtgt tccgtgctgc gatggaacgc ggcgacgtgg aggttttggc gatcaacgac ccgttcatgt ccctggacta catggtgtac cttctgagat acgactcggt ccacggccac taccccggcg aagtcagcca caaggacggc aagctcattg tcggcggaaa ggccgtgact gtcttcaacg agaaggagcc aactgccatc ccttggggcc aggctggcgt ccactacatc tgcgagtcca ccggtatttt cctcaccaaa gagaaggctc aggctcactt gaccaacggc gcgaagaagg tcatcatgtc cgcccccccc aaggacgaca ccccgatgtt cgtcatgggt gtcaacaatg accagtacaa gtccagcgat gtcattgtgt cgaacgcctc ttgcacgacc aactgcttgg ctcctctcgc caaaattgtc cacgataagt tcggcatcgt tgagggtctc atgaccaccg tccatgcgat gacggcgaac cagctgacgg ttgatggacc ctccaagggt ggcaaagatt ggcgcgctgg ccggtcggca ggcgtcaaca tcatccctgc ctccactggt gccgccaagg ctgtgggcaa gattattcct tccctgaacg gcaagctgac cggtatggct ttccgtgtgc ccgtccccga cgtgtctgtg gttgatctga cctgcaagct ggccaagccc gcgaagtacg aggacatcgt ggcagctgtg aaggaggctg ccacctccgg ccccatgaag ggaatcatca gctacaccga cgaagaggtc gtctcctctg acttcgtcca ctgcaagttc tcctccgtgt ttgacatcaa cgccggcatc atgcttaacg acaccttcgt taagctcgtg tcttggtacg acaacgagtg gggatactcc aaccgccttg ttgagctggc tcactacatg tccgtccagg acggcgcgaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for TogoA.00914.a.A1.PS01526
| PURIFICATION DATe: | 6/15/2012 |
| CONCENTRATION: | 90.6mg/ml |
| OBSERVED MW: | 37kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVCKLGING FGRIGRLVFR AAMERGDVEV LAINDPFMSL DYMVYLLRYD SVHGHYPGEV SHKDGKLIVG GKAVTVFNEK EPTAIPWGQA GVHYICESTG IFLTKEKAQA HLTNGAKKVI MSAPPKDDTP MFVMGVNNDQ YKSSDVIVSN ASCTTNCLAP LAKIVHDKFG IVEGLMTTVH AMTANQLTVD GPSKGGKDWR AGRSAGVNII PASTGAAKAV GKIIPSLNGK LTGMAFRVPV PDVSVVDLTC KLAKPAKYED IVAAVKEAAT SGPMKGIISY TDEEVVSSDF VHCKFSSVFD INAGIMLNDT FVKLVSWYDN EWGYSNRLVE LAHYMSVQDG A
Validated NT Sequence
ttattacgcg ccgtcctgga cggacatgta gtgagccagc tcaacaaggc ggttggagta tccccactcg ttgtcgtacc aagacacgag cttaacgaag gtgtcgttaa gcatgatgcc ggcgttgatg tcaaacacgg aggagaactt gcagtggacg aagtcagagg agacgacctc ttcgtcggtg tagctgatga ttcccttcat ggggccggag gtggcagcct ccttcacagc tgccacgatg tcctcgtact tcgcgggctt ggccagcttg caggtcagat caaccacaga cacgtcgggg acgggcacac ggaaagccat accggtcagc ttgccgttca gggaaggaat aatcttgccc acagccttgg cggcaccagt ggaggcaggg atgatgttga cgcctgccga ccggccagcg cgccaatctt tgccaccctt ggagggtcca tcaaccgtca gctggttcgc cgtcatcgca tggacggtgg tcatgagacc ctcaacgatg ccgaacttat cgtggacaat tttggcgaga ggagccaagc agttggtcgt gcaagaggcg ttcgacacaa tgacatcgct ggacttgtac tggtcattgt tgacacccat gacgaacatc ggggtgtcgt ccttgggggg ggcggacatg atgaccttct tcgcgccgtt ggtcaagtga gcctgagcct tctctttggt aaggaaaata ccggtggact cgcagatgta gtggacgcca gcctggcccc aagggatggc agttggctcc ttctcgttga agacagtcac ggcctttccg ccgacaatga gcttgccgtc cttgtggctg acttcgccgg ggtagtggcc gtggaccgag tcgtatctca gaaggtacac catgtagtcc agggacatga acgggtcgtt gatcgccaaa acctccacgt cgccgcgttc catcgcagca cggaacacca gacggccaat acggccaaaa ccgttaatac ccagcttgca caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtatatctcc tt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVCKLGING FGRIGRLVFR AAMERGDVEV LAINDPFMSL DYMVYLLRYD SVHGHYPGEV SHKDGKLIVG GKAVTVFNEK EPTAIPWGQA GVHYICESTG IFLTKEKAQA HLTNGAKKVI MSAPPKDDTP MFVMGVNNDQ YKSSDVIVSN ASCTTNCLAP LAKIVHDKFG IVEGLMTTVH AMTANQLTVD GPSKGGKDWR AGRSAGVNII PASTGAAKAV GKIIPSLNGK LTGMAFRVPV PDVSVVDLTC KLAKPAKYED IVAAVKEAAT SGPMKGIISY TDEEVVSSDF VHCKFSSVFD INAGIMLNDT FVKLVSWYDN EWGYSNRLVE LAHYMSVQDG A
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTGCAAG CTGGGTATTA ACGGTTTTGG CCGTATTGGC CGTCTGGTGT TCCGTGCTGC GATGGAACGC GGCGACGTGG AGGTTTTGGC GATCAACGAC CCGTTCATGT CCCTGGACTA CATGGTGTAC CTTCTGAGAT ACGACTCGGT CCACGGCCAC TACCCCGGCG AAGTCAGCCA CAAGGACGGC AAGCTCATTG TCGGCGGAAA GGCCGTGACT GTCTTCAACG AGAAGGAGCC AACTGCCATC CCTTGGGGCC AGGCTGGCGT CCACTACATC TGCGAGTCCA CCGGTATTTT CCTCACCAAA GAGAAGGCTC AGGCTCACTT GACCAACGGC GCGAAGAAGG TCATCATGTC CGCCCCCCCC AAGGACGACA CCCCGATGTT CGTCATGGGT GTCAACAATG ACCAGTACAA GTCCAGCGAT GTCATTGTGT CGAACGCCTC TTGCACGACC AACTGCTTGG CTCCTCTCGC CAAAATTGTC CACGATAAGT TCGGCATCGT TGAGGGTCTC ATGACCACCG TCCATGCGAT GACGGCGAAC CAGCTGACGG TTGATGGACC CTCCAAGGGT GGCAAAGATT GGCGCGCTGG CCGGTCGGCA GGCGTCAACA TCATCCCTGC CTCCACTGGT GCCGCCAAGG CTGTGGGCAA GATTATTCCT TCCCTGAACG GCAAGCTGAC CGGTATGGCT TTCCGTGTGC CCGTCCCCGA CGTGTCTGTG GTTGATCTGA CCTGCAAGCT GGCCAAGCCC GCGAAGTACG AGGACATCGT GGCAGCTGTG AAGGAGGCTG CCACCTCCGG CCCCATGAAG GGAATCATCA GCTACACCGA CGAAGAGGTC GTCTCCTCTG ACTTCGTCCA CTGCAAGTTC TCCTCCGTGT TTGACATCAA CGCCGGCATC ATGCTTAACG ACACCTTCGT TAAGCTCGTG TCTTGGTACG ACAACGAGTG GGGATACTCC AACCGCCTTG TTGAGCTGGC TCACTACATG TCCGTCCAGG ACGGCGCGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA