TogoA.00877.b

TgCentrin2

CENTER ID:	TogoA.00877.b
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.00877.b.A2.GE30691	from TgCentrin2 construct		74	241

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_88701

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_250340
OrthoMCL:	OG5_138807
RefSeq:	XP_002367347.1
UniProt:	B6KHM3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLIYVKTVLE EQEPIYPIEG KTVLCWTECH FLDEGSRSSW ANGCLECEKP VANPLDTGYT ESPLSLHSLA PKRRGALRGA SPTARRRLVD RPGLTEDEIE EIREAFNLFD TDGSGMIDPK ELKAAMQSLG FETKNPTIYQ MIADLDRDSG GPIDFEEFLD AITAKLGDKE SREGIQKIFS LFDDDRTGTI TLKNLKRVAK ELGETMSEDE LREMLERADS NGDGEISFED FYAIMTKKTF P

NT Sequence

atgctcatat acgtcaagac agtgctggag gagcaggaac cgatatatcc gatagaggga aaaactgttt tgtgctggac agaatgccac tttcttgatg aggggtcaag gagctcgtgg gccaacgggt gccttgagtg tgaaaagcca gtcgcgaatc cgttagacac agggtacaca gaaagcccgt tatcacttca ctcccttgct ccaaaaagac gaggagcact gcgaggggcg agcccgacag ccaggcgtcg actagttgat cggcctggac taacagagga cgaaatcgag gagatacgcg aagctttcaa cctctttgac acagacggca gtggtatgat cgatccgaaa gaactaaagg ccgccatgca gtctctcggc tttgaaacaa agaatccgac gatctatcaa atgatcgcag atctcgaccg cgactccggc ggacccatcg acttcgagga atttcttgac gccattaccg cgaagctggg tgacaaggaa agccgcgaag gaatccagaa gattttcagc ctcttcgacg acgacagaac aggaacaatt acactaaaga atctcaagcg tgtagcaaag gaattgggag agacgatgtc tgaagacgag cttcgtgaga tgcttgagcg agcggactca aatggcgacg gggagatttc cttcgaggat ttttatgcga tcatgaccaa gaagactttc ccgtag

Details for TogoA.00877.b.A2.GE30691

HARVESTED ON:	data unavailable
SEQUENCED ON:	9/28/2010
EXPECTED MW:	21kDa
OBSERVED MW:	17kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRGALRGAS PTARRRLVDR PGLTEDEIEE IREAFNLFDT DGSGMIDPKE LKAAMQSLGF ETKNPTIYQM IADLDRDSGG PIDFEEFLDA ITAKLGDKES REGIQKIFSL FDDDRTGTIT LKNLKRVAKE LGETMSEDEL REMLERADSN GDGEISFEDF YAIMTKKTFP

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgag gagcactgcg aggggcgagc ccgacagcca ggcgtcgact agttgatcgg cctggactaa cagaggacga aatcgaggag atacgcgaag ctttcaacct ctttgacaca gacggcagtg gtatgatcga tccgaaagaa ctaaaggccg ccatgcagtc tctcggcttt gaaacaaaga atccgacgat ctatcaaatg atcgcagatc tcgaccgcga ctccggcgga cccatcgact tcgaggaatt tcttgacgcc attaccgcga agctgggtga caaggaaagc cgcgaaggaa tccagaagat tttcagcctc ttcgacgacg acagaacagg aacaattaca ctaaagaatc tcaagcgtgt agcaaaggaa ttgggagaga cgatgtctga agacgagctt cgtgagatgc ttgagcgagc ggactcaaat ggcgacgggg agatttcctt cgaggatttt tatgcgatca tgaccaagaa gactttcccg taataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgaggagca ctgcgagggg cgagcccgac agccaggcgt cgactagttg atcggcctgg actaacagag gacgaaatcg aggagatacg cgaagctttc aacctctttg acacagacgg cagtggtatg atcgatccga aagaactaaa ggccgccatg cagtctctcg gctttgaaac aaagaatccg acgatctatc aaatgatcgc agatctcgac cgcgactccg gcggacccat cgacttcgag gaatttcttg acgccattac cgcgaagctg ggtgacaagg aaagccgcga aggaatccag aagattttca gcctcttcga cgacgacaga acaggaacaa ttacactaaa gaatctcaag cgtgtagcaa aggaattggg agagacgatg tctgaagacg agcttcgtga gatgcttgag cgagcggact caaatggcga cggggagatt tccttcgagg atttttatgc gatcatgacc aagaagactt tcccgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa