SSGCID: Target Page

TogoA.00550.a

Intracellular protease, putative

CENTER ID:	TogoA.00550.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.00550.a.A2.GE33599	mature protein		21	256

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_214290
RefSeq:	XP_002370762.1
UniProt:	B6KSD9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSPLGFGAFL SPLLLFCVSP HLRTGVSRSF SSFLKPQKLQ RFVSREYPRR RCLPSLHFTS RCFSTRLSSR KMAVKVLVPV AHDSEEIEAV SIIDTLRRAG AEVVVASVED TEIVRMSRGV CVKADKLISA VENETYDCIA IPGGMPGAER CRDSAALTAM LKTHKAQGKL IAAICASPAV VLQTHGLLQG EKAVAYPCFM DQFPADMRGE GRVCVSNKIV TSVGPSSAIE FALKLIEVLY NKEQAKKIAA QLLYAY

NT Sequence

atgtctccgc taggcttcgg agcgtttctt tcccctctgc ttctcttttg tgtgtctcct cacttgcgga cgggagtttc ccgatctttc tcttctttct tgaagccaca aaagctccag cgttttgttt ctcgcgagta tccccgtcgc cgttgtctgc cgtctctcca tttcacgtct cggtgttttt ccacgcgtct ttcctcgaga aaaatggcag tcaaagtgct tgttcccgtc gctcacgata gcgaggaaat tgaggcagtc tccatcattg atacccttcg acgagccggc gccgaggtgg tggttgcgag tgtagaggac accgaaatcg ttcgcatgtc acgcggcgtt tgcgtgaaag ccgacaagct catctcagcg gtggaaaatg aaacgtatga ctgcatcgcg attccgggtg gcatgccagg ggccgaaagg tgccgagact ctgcagctct gacggccatg ctcaagactc acaaggcaca gggcaagctc attgccgcga tctgcgccag ccccgctgtc gtgctccaga ctcacggtct tcttcaagga gaaaaggcgg ttgcgtatcc ctgcttcatg gaccaattcc ccgccgatat gcgtggcgaa ggccgagtct gcgtcagcaa caaaatcgtg acttccgttg gaccctcgag cgctatcgaa ttcgcactga aactcatcga agttctgtac aacaaagagc aggcgaagaa gatcgcggca cagctccttt acgcgtacta a

Details for TogoA.00550.a.A2.GE33599

HARVESTED ON:	10/31/2011
SEQUENCED ON:	11/4/2011
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	109

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMHLRTGVSR SFSSFLKPQK LQRFVSREYP RRRCLPSLHF TSRCFSTRLS SRKMAVKVLV PVAHDSEEIE AVSIIDTLRR AGAEVVVASV EDTEIVRMSR GVCVKADKLI SAVENETYDC IAIPGGMPGA ERCRDSAALT AMLKTHKAQG KLIAAICASP AVVLQTHGLL QGEKXVAYPC FMDQFPADMR GEXRVCVSNK IVTSVGPSSA IEFALKLIEV LYNKEXXRRS RHSSFTRTNK QHEQVLQXXF SRIXLLXKPE XX

Validated NT Sequence

attttgttnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcact tgcggacggg agtttcccga tctttctctt ctttcttgaa gccacaaaag ctccagcgtt ttgtttctcg cgagtatccc cgtcgccgtt gtctgccgtc tctccatttc acgtctcggt gtttttccac gcgtctttcc tcgagaaaaa tggcagtcaa agtgcttgtt cccgtcgctc acgatagcga ggaaattgag gcagtctcca tcattgatac ccttcgacga gccggcgccg aggtggtggt tgcgagtgta gaggacaccg aaatcgttcg catgtcacgc ggcgtttgcg tgaaagccga caagctcatc tcagcggtgg aaaatgaaac gtatgactgc atcgcgattc cgggtggcat gccaggggcc gaaaggtgcc gagactctgc agctctgacg gccatgctca agactcacaa ggcacagggc aagctcattg ccgcgatctg cgccagcccc gctgtcgtgc tccagactca cggtcttctt caaggagaaa aggnggttgc gtatccctgc ttcatggacc aattccccgc cgatatgcgt ggcgaangnc gagtctgcgt cagcaacaaa atcgtgactt ccgttggacc ctcgagcgct atcgaattcg cactgaaact catcgaagtt ctgtacaaca aagagcnnng aagaagatcg cggcacagct cctttacgcg tactaataaa cagcacgaac aagttctgca gncnancttc tcgaggatcc ngctgctann aaagcccgaa annanctgat tggctgctgc cnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMHLRTGVSR SFSSFLKPQK LQRFVSREYP RRRCLPSLHF TSRCFSTRLS SRKMAVKVLV PVAHDSEEIE AVSIIDTLRR AGAEVVVASV EDTEIVRMSR GVCVKADKLI SAVENETYDC IAIPGGMPGA ERCRDSAALT AMLKTHKAQG KLIAAICASP AVVLQTHGLL QGEKAVAYPC FMDQFPADMR GEGRVCVSNK IVTSVGPSSA IEFALKLIEV LYNKEQAKKI AAQLLYAY

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcacttgcgg acgggagttt cccgatcttt ctcttctttc ttgaagccac aaaagctcca gcgttttgtt tctcgcgagt atccccgtcg ccgttgtctg ccgtctctcc atttcacgtc tcggtgtttt tccacgcgtc tttcctcgag aaaaatggca gtcaaagtgc ttgttcccgt cgctcacgat agcgaggaaa ttgaggcagt ctccatcatt gatacccttc gacgagccgg cgccgaggtg gtggttgcga gtgtagagga caccgaaatc gttcgcatgt cacgcggcgt ttgcgtgaaa gccgacaagc tcatctcagc ggtggaaaat gaaacgtatg actgcatcgc gattccgggt ggcatgccag gggccgaaag gtgccgagac tctgcagctc tgacggccat gctcaagact cacaaggcac agggcaagct cattgccgcg atctgcgcca gccccgctgt cgtgctccag actcacggtc ttcttcaagg agaaaaggcg gttgcgtatc cctgcttcat ggaccaattc cccgccgata tgcgtggcga aggccgagtc tgcgtcagca acaaaatcgt gacttccgtt ggaccctcga gcgctatcga attcgcactg aaactcatcg aagttctgta caacaaagag caggcgaaga agatcgcggc acagctcctt tacgcgtact aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa