TogoA.00487.a

Dihydroorotate dehydrogenase (DHOD)

CENTER ID: TogoA.00487.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.00487.a.B21.GE42561 VSSM N-term truncation (Hortua et al) 178 592
TogoA.00487.a.B41.GE42563 vssm-khkh 178 580
TogoA.00487.a.B61.GE42565 miys 181 592
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.00487.a.B21.PS38547 VSSM N-term truncation (Hortua et al) 178 592
TogoA.00487.a.B21.PS38563 VSSM N-term truncation (Hortua et al) 178 592
TogoA.00487.a.B21.PS38564 VSSM N-term truncation (Hortua et al) 178 592

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_210790
UniProt: Q8MXD1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAPLTMHFQG RFALLRLPIS SGKPLCRETR VRRSGTRPVS ADNLSHARCV LPKCHSFCPA GGMQESPEAR VTLSRGTSRN FGTFLTALGN DVHWKSAFPG ALLRTQIRKL SVSLHPRPGS AESSRPSAGL PPKDVDPEEI ERIVRERTTR ERKANRRLVF LVLLLGTGVY CYSALQDVSS MIYSFYEPVT SVLFRYFSSG PLDPETAHGY TMELAKRGWL PVDYDREESA LNVDINGLKF LSPIGLAAGF DKHAEAPAAL LRMGFSFLEV GSITPKPQPG NPKPRLFRLY EDRSVINRFG FNSNGADYAQ TQLEAFSEAR LRDPFTAQGV LGVSLGKNKT SEDAVADLRE GVKKLGRFAD FLVVNLSSPN TPGLRSLQSA SHLAAIIDGV QEELDALDRQ AQAASQKQRN ERRRHGGNPE ETKAFYANQT GRRPLFFVKI APDLSMEEKE SIAKVALEKN LDGFVVSNTT IQRPETLKSP AKSETGGLSG RALKHLSTAC VSDMYKLTQG KLAIIATGGV ESGRDALDKI EAGASLVELY SSMVYIGPQV ARRVKNELYH ALNEKGYKDV AAAVGRKHKH VPEKKLQAPK FD
NT Sequence
atggcacctc ttaccatgca tttccaaggg cgtttcgcct tgctgcggct gccaatctct tcaggcaagc cgctctgcag ggaaacccgc gtgcgacgaa gtggaactcg acccgtttcc gccgacaacc tgtcgcatgc gagatgtgtc ctgccgaaat gccattcgtt ttgtccggcg ggcggaatgc aagaaagtcc cgaggcgaga gtcactcttt ctagaggcac cagccggaat ttcggaacct ttctaaccgc tctggggaac gatgtgcact ggaaatcggc gtttcccggt gctttgctgc gcacgcaaat ccgaaagctc agcgtgtctc tacacccgag gccaggcagc gcggagagca gcagacccag cgcgggtctc ccgccgaagg acgtagatcc agaagaaatc gagagaatcg ttcgggaacg aacaactcga gagagaaaag cgaatcggcg cctggtcttc ctcgtccttc ttctcggcac cggagtgtac tgctacagcg ctctgcaaga cgtgagcagt atgatctact ccttctacga gccggtaaca tccgtcttat ttcgttactt cagcagcggc ccactggacc cggaaacggc tcacgggtac accatggaac ttgcgaaaag aggctggctc ccagtcgact acgacagaga agagagcgca ctgaacgtgg acatcaatgg tttgaagttc ctcagcccca tcggcctcgc tgcgggattc gacaagcacg ctgaagcacc ggcagctctt ttgaggatgg gattttcctt cctcgaagtg ggttcgatca cgcctaagcc tcaacctgga aatccgaaac ctcgtctgtt tcgactttac gaagatcggt cggtcatcaa tcgcttcgga ttcaacagca atggcgctga ctacgctcag acgcagctgg aagccttctc agaggcccga ctcagagatc ccttcacagc gcagggggtg ctgggagtga gtttaggaaa gaacaaaacg agcgaggatg cggtggcaga cttgcgcgag ggcgtgaaga aactggggag atttgcggac tttctcgtcg tgaatctttc ttctcccaac acaccgggtc tgagaagtct tcaaagcgct tcacatttgg ctgcgatcat cgatggggtt caggaggaac tcgacgccct agaccgacag gctcaggcag ctagccagaa gcagcgaaac gaaaggcgaa ggcatggagg aaatcctgaa gaaacgaaag ccttctacgc caaccaaact ggaaggagac ctcttttctt cgtcaaaatc gcccctgacc tctcgatgga agagaaggaa agcatcgcaa aggttgctct cgagaagaac ctcgatggat tcgtcgtctc taacacgact atccagcgac cagagacctt gaagagtcct gccaagtcgg agactggcgg tctcagcggc cgggctctga aacatttgtc gacagcatgc gtttcggata tgtacaaact gactcaaggg aaactcgcta tcatcgcgac aggaggcgtt gaaagcggtc gtgatgcgct agataaaatc gaagcaggcg cgtctctcgt cgaactctac agcagcatgg tctacatcgg cccacaagtt gcgcgccgtg tgaagaacga actctaccac gcgctgaatg agaaaggata caaagatgtc gccgccgctg ttggacgaaa gcacaagcat gttccagaga aaaagctgca ggctccgaaa ttcgactga
Details for TogoA.00487.a.B21.GE42561
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHVS SMIYSFYEPV TSVLFRYFSS GPLDPETAHG YTMELAKRGW LPVDYDREES ALNVDINGLK FLSPIGLAAG FDKHAEAPAA LLRMGFSFLE VGSITPKPQP GNPKPRLFRL YEDRSVINRF GFNSNGADYA QTQLEAFSEA RLRDPFTAQG VLGVSLGKNK TSEDAVADLR EGVKKLGRFA DFLVVNLSSP NTPGLRSLQS ASHLAAIIDG VQEELDALDR QAQAASQKQR NERRRHGGNP EETKAFYANQ TGRRPLFFVK IAPDLSMEEK ESIAKVALEK NLDGFVVSNT TIQRPETLKS PAKSETGGLS GRALKHLSTA CVSDMYKLTQ GKLAIIATGG VESGRDALDK IEAGASLVEL YSSMVYIGPQ VARRVKNELY HALNEKGYKD VAAAVGRKHK HVPEKKLQAP KFD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgttagca gcatgatcta tagcttttat gaaccggtta ccagcgttct gtttcgttat ttcagcagcg gtccgctgga cccggaaacc gcacatggtt ataccatgga actggcaaaa cgtggttggc tgccggttga ttatgatcgt gaagaaagcg cactgaacgt ggatattaat ggtctgaaat ttctgagccc gattggtctg gcagcaggtt ttgataaaca tgcagaagca ccggcagcac tgctgcgtat gggttttagc tttctggaag ttggtagcat tacaccgaaa ccgcagcctg gtaatccgaa acctcgtctg tttcgcctgt atgaagatcg tagcgttatt aatcgctttg gctttaatag caacggtgca gattatgcac agacccagct ggaagcattt agcgaagcac gtctgcgtga tccgtttacc gcacagggtg ttctgggtgt tagcctgggt aaaaacaaaa ccagcgaaga tgcagttgcc gatctgcgtg aaggtgttaa aaaactgggt cgttttgcag attttctggt tgttaatctg agcagcccga atacaccggg tctgcgtagc ctgcagagcg caagccatct ggcagccatt attgatggtg ttcaagagga actggatgca ctggatcgtc aggcacaggc agcaagccag aaacagcgta atgaacgtcg tcgtcatggt ggcaatccgg aagaaaccaa agcattttat gcaaatcaga ccggtcgtcg tccgctgttt tttgttaaaa ttgcaccgga tctgagcatg gaagaaaaag aaagcattgc aaaagtggcc ctggaaaaaa atctggatgg ttttgtggtt agcaacacca ccattcagcg tccggaaaca ctgaaaagtc cggcaaaaag cgaaaccggt ggtctgagcg gtcgtgcact gaaacatctg agcaccgcat gtgttagcga tatgtataaa ctgacccagg gtaaactggc aattattgcg accggtggtg tggaaagtgg tcgtgatgcc ctggataaaa ttgaagccgg tgcaagcctg gttgaactgt atagcagtat ggtttatatt ggtccgcagg ttgcacgtcg tgtgaaaaac gaactgtatc atgcactgaa tgagaaaggc tataaagatg ttgcagcagc agttggtcgt aaacataaac atgttccgga aaaaaaactg caggcaccga aatttgattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for TogoA.00487.a.B41.GE42563
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 45kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHVS SMIYSFYEPV TSVLFRYFSS GPLDPETAHG YTMELAKRGW LPVDYDREES ALNVDINGLK FLSPIGLAAG FDKHAEAPAA LLRMGFSFLE VGSITPKPQP GNPKPRLFRL YEDRSVINRF GFNSNGADYA QTQLEAFSEA RLRDPFTAQG VLGVSLGKNK TSEDAVADLR EGVKKLGRFA DFLVVNLSSP NTPGLRSLQS ASHLAAIIDG VQEELDALDR QAQAASQKQR NERRRHGGNP EETKAFYANQ TGRRPLFFVK IAPDLSMEEK ESIAKVALEK NLDGFVVSNT TIQRPETLKS PAKSETGGLS GRALKHLSTA CVSDMYKLTQ GKLAIIATGG VESGRDALDK IEAGASLVEL YSSMVYIGPQ VARRVKNELY HALNEKGYKD VAAAVGRKHK H
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgttagca gcatgatcta tagcttttat gaaccggtta ccagcgttct gtttcgttat ttcagcagcg gtccgctgga cccggaaacc gcacatggtt ataccatgga actggcaaaa cgtggttggc tgccggttga ttatgatcgt gaagaaagcg cactgaacgt ggatattaat ggtctgaaat ttctgagccc gattggtctg gcagcaggtt ttgataaaca tgcagaagca ccggcagcac tgctgcgtat gggttttagc tttctggaag ttggtagcat tacaccgaaa ccgcagcctg gtaatccgaa acctcgtctg tttcgcctgt atgaagatcg tagcgttatt aatcgctttg gctttaatag caacggtgca gattatgcac agacccagct ggaagcattt agcgaagcac gtctgcgtga tccgtttacc gcacagggtg ttctgggtgt tagcctgggt aaaaacaaaa ccagcgaaga tgcagttgcc gatctgcgtg aaggtgttaa aaaactgggt cgttttgcag attttctggt tgttaatctg agcagcccga atacaccggg tctgcgtagc ctgcagagcg caagccatct ggcagccatt attgatggtg ttcaagagga actggatgca ctggatcgtc aggcacaggc agcaagccag aaacagcgta atgaacgtcg tcgtcatggt ggcaatccgg aagaaaccaa agcattttat gcaaatcaga ccggtcgtcg tccgctgttt tttgttaaaa ttgcaccgga tctgagcatg gaagaaaaag aaagcattgc aaaagtggcc ctggaaaaaa atctggatgg ttttgtggtt agcaacacca ccattcagcg tccggaaaca ctgaaaagtc cggcaaaaag cgaaaccggt ggtctgagcg gtcgtgcact gaaacatctg agcaccgcat gtgttagcga tatgtataaa ctgacccagg gtaaactggc aattattgcg accggtggtg tggaaagtgg tcgtgatgcc ctggataaaa ttgaagccgg tgcaagcctg gttgaactgt atagcagtat ggtttatatt ggtccgcagg ttgcacgtcg tgtgaaaaac gaactgtatc atgcactgaa tgagaaaggc tataaagatg ttgcagcagc agttggtcgt aaacataaac attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for TogoA.00487.a.B61.GE42565
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMI YSFYEPVTSV LFRYFSSGPL DPETAHGYTM ELAKRGWLPV DYDREESALN VDINGLKFLS PIGLAAGFDK HAEAPAALLR MGFSFLEVGS ITPKPQPGNP KPRLFRLYED RSVINRFGFN SNGADYAQTQ LEAFSEARLR DPFTAQGVLG VSLGKNKTSE DAVADLREGV KKLGRFADFL VVNLSSPNTP GLRSLQSASH LAAIIDGVQE ELDALDRQAQ AASQKQRNER RRHGGNPEET KAFYANQTGR RPLFFVKIAP DLSMEEKESI AKVALEKNLD GFVVSNTTIQ RPETLKSPAK SETGGLSGRA LKHLSTACVS DMYKLTQSKL AIIATGGVES GRDALDKIEA GASLVELYSS MVYIGPQVAR RVKNELYHAL NEKGYKDVAA AVGRKHKHVP EK
Validated NT Sequence
atggctcacc accaccacca ccatatgatc tatagctttt atgaaccggt taccagcgtt ctgtttcgtt atttcagcag cggtccgctg gacccggaaa ccgcacatgg ttataccatg gaactggcaa aacgtggttg gctgccggtt gattatgatc gtgaagaaag cgcactgaac gtggatatta atggtctgaa atttctgagc ccgattggtc tggcagcagg ttttgataaa catgcagaag caccggcagc actgctgcgt atgggtttta gctttctgga agttggtagc attacaccga aaccgcagcc tggtaatccg aaacctcgtc tgtttcgcct gtatgaagat cgtagcgtta ttaatcgctt tggctttaat agcaacggtg cagattatgc acagacccag ctggaagcat ttagcgaagc acgtctgcgt gatccgttta ccgcacaggg tgttctgggt gttagcctgg gtaaaaacaa aaccagcgaa gatgcagttg ccgatctgcg tgaaggtgtt aaaaaactgg gtcgttttgc agattttctg gttgttaatc tgagcagccc gaatacaccg ggtctgcgta gcctgcagag cgcaagccat ctggcagcca ttattgatgg tgttcaagag gaactggatg cactggatcg tcaggcacag gcagcaagcc agaaacagcg taatgaacgt cgtcgtcatg gtggcaatcc ggaagaaacc aaagcatttt atgcaaatca gaccggtcgt cgtccgctgt tttttgttaa aattgcaccg gatctgagca tggaagaaaa agaaagcatt gcaaaagtgg ccctggaaaa aaatctggat ggttttgtgg ttagcaacac caccattcag cgtccggaaa cactgaaaag tccggcaaaa agcgaaaccg gtggtctgag cggtcgtgca ctgaaacatc tgagcaccgc atgtgttagc gatatgtata aactgaccca aagtaaactg gcaattattg cgaccggtgg tgtggaaagt ggtcgtgatg ccctggataa aattgaagcc ggtgcaagcc tggttgaact gtatagcagt atggtttata ttggtccgca ggttgcacgt cgtgtgaaaa acgaactgta tcatgcactg aatgagaaag gctataaaga tgttgcagca gcagttggtc gtaaacataa acatgttccg gaaaaaaa
Expected Protein Sequence
MAHHHHHHMI YSFYEPVTSV LFRYFSSGPL DPETAHGYTM ELAKRGWLPV DYDREESALN VDINGLKFLS PIGLAAGFDK HAEAPAALLR MGFSFLEVGS ITPKPQPGNP KPRLFRLYED RSVINRFGFN SNGADYAQTQ LEAFSEARLR DPFTAQGVLG VSLGKNKTSE DAVADLREGV KKLGRFADFL VVNLSSPNTP GLRSLQSASH LAAIIDGVQE ELDALDRQAQ AASQKQRNER RRHGGNPEET KAFYANQTGR RPLFFVKIAP DLSMEEKESI AKVALEKNLD GFVVSNTTIQ RPETLKSPAK SETGGLSGRA LKHLSTACVS DMYKLTQGKL AIIATGGVES GRDALDKIEA GASLVELYSS MVYIGPQVAR RVKNELYHAL NEKGYKDVAA AVGRKHKHVP EKKLQAPKFD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatct atagctttta tgaaccggtt accagcgttc tgtttcgtta tttcagcagc ggtccgctgg acccggaaac cgcacatggt tataccatgg aactggcaaa acgtggttgg ctgccggttg attatgatcg tgaagaaagc gcactgaacg tggatattaa tggtctgaaa tttctgagcc cgattggtct ggcagcaggt tttgataaac atgcagaagc accggcagca ctgctgcgta tgggttttag ctttctggaa gttggtagca ttacaccgaa accgcagcct ggtaatccga aacctcgtct gtttcgcctg tatgaagatc gtagcgttat taatcgcttt ggctttaata gcaacggtgc agattatgca cagacccagc tggaagcatt tagcgaagca cgtctgcgtg atccgtttac cgcacagggt gttctgggtg ttagcctggg taaaaacaaa accagcgaag atgcagttgc cgatctgcgt gaaggtgtta aaaaactggg tcgttttgca gattttctgg ttgttaatct gagcagcccg aatacaccgg gtctgcgtag cctgcagagc gcaagccatc tggcagccat tattgatggt gttcaagagg aactggatgc actggatcgt caggcacagg cagcaagcca gaaacagcgt aatgaacgtc gtcgtcatgg tggcaatccg gaagaaacca aagcatttta tgcaaatcag accggtcgtc gtccgctgtt ttttgttaaa attgcaccgg atctgagcat ggaagaaaaa gaaagcattg caaaagtggc cctggaaaaa aatctggatg gttttgtggt tagcaacacc accattcagc gtccggaaac actgaaaagt ccggcaaaaa gcgaaaccgg tggtctgagc ggtcgtgcac tgaaacatct gagcaccgca tgtgttagcg atatgtataa actgacccag ggtaaactgg caattattgc gaccggtggt gtggaaagtg gtcgtgatgc cctggataaa attgaagccg gtgcaagcct ggttgaactg tatagcagta tggtttatat tggtccgcag gttgcacgtc gtgtgaaaaa cgaactgtat catgcactga atgagaaagg ctataaagat gttgcagcag cagttggtcg taaacataaa catgttccgg aaaaaaaact gcaggcaccg aaatttgatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for TogoA.00487.a.B21.PS38547
PURIFICATION DATe: 12/12/2018
CONCENTRATION: 2.54mg/ml
OBSERVED MW: 46kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP, 0.25mM Orotic Acid
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHVS SMIYSFYEPV TSVLFRYFSS GPLDPETAHG YTMELAKRGW LPVDYDREES ALNVDINGLK FLSPIGLAAG FDKHAEAPAA LLRMGFSFLE VGSITPKPQP GNPKPRLFRL YEDRSVINRF GFNSNGADYA QTQLEAFSEA RLRDPFTAQG VLGVSLGKNK TSEDAVADLR EGVKKLGRFA DFLVVNLSSP NTPGLRSLQS ASHLAAIIDG VQEELDALDR QAQAASQKQR NERRRHGGNP EETKAFYANQ TGRRPLFFVK IAPDLSMEEK ESIAKVALEK NLDGFVVSNT TIQRPETLKS PAKSETGGLS GRALKHLSTA CVSDMYKLTQ GKLAIIATGG VESGRDALDK IEAGASLVEL YSSMVYIGPQ VARRVKNELY HALNEKGYKD VAAAVGRKHK HVPEKKLQAP KFD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTTAGCA GCATGATCTA TAGCTTTTAT GAACCGGTTA CCAGCGTTCT GTTTCGTTAT TTCAGCAGCG GTCCGCTGGA CCCGGAAACC GCACATGGTT ATACCATGGA ACTGGCAAAA CGTGGTTGGC TGCCGGTTGA TTATGATCGT GAAGAAAGCG CACTGAACGT GGATATTAAT GGTCTGAAAT TTCTGAGCCC GATTGGTCTG GCAGCAGGTT TTGATAAACA TGCAGAAGCA CCGGCAGCAC TGCTGCGTAT GGGTTTTAGC TTTCTGGAAG TTGGTAGCAT TACACCGAAA CCGCAGCCTG GTAATCCGAA ACCTCGTCTG TTTCGCCTGT ATGAAGATCG TAGCGTTATT AATCGCTTTG GCTTTAATAG CAACGGTGCA GATTATGCAC AGACCCAGCT GGAAGCATTT AGCGAAGCAC GTCTGCGTGA TCCGTTTACC GCACAGGGTG TTCTGGGTGT TAGCCTGGGT AAAAACAAAA CCAGCGAAGA TGCAGTTGCC GATCTGCGTG AAGGTGTTAA AAAACTGGGT CGTTTTGCAG ATTTTCTGGT TGTTAATCTG AGCAGCCCGA ATACACCGGG TCTGCGTAGC CTGCAGAGCG CAAGCCATCT GGCAGCCATT ATTGATGGTG TTCAAGAGGA ACTGGATGCA CTGGATCGTC AGGCACAGGC AGCAAGCCAG AAACAGCGTA ATGAACGTCG TCGTCATGGT GGCAATCCGG AAGAAACCAA AGCATTTTAT GCAAATCAGA CCGGTCGTCG TCCGCTGTTT TTTGTTAAAA TTGCACCGGA TCTGAGCATG GAAGAAAAAG AAAGCATTGC AAAAGTGGCC CTGGAAAAAA ATCTGGATGG TTTTGTGGTT AGCAACACCA CCATTCAGCG TCCGGAAACA CTGAAAAGTC CGGCAAAAAG CGAAACCGGT GGTCTGAGCG GTCGTGCACT GAAACATCTG AGCACCGCAT GTGTTAGCGA TATGTATAAA CTGACCCAGG GTAAACTGGC AATTATTGCG ACCGGTGGTG TGGAAAGTGG TCGTGATGCC CTGGATAAAA TTGAAGCCGG TGCAAGCCTG GTTGAACTGT ATAGCAGTAT GGTTTATATT GGTCCGCAGG TTGCACGTCG TGTGAAAAAC GAACTGTATC ATGCACTGAA TGAGAAAGGC TATAAAGATG TTGCAGCAGC AGTTGGTCGT AAACATAAAC ATGTTCCGGA AAAAAAACTG CAGGCACCGA AATTTGATTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT
Details for TogoA.00487.a.B21.PS38563
PURIFICATION DATe: 3/27/2019
CONCENTRATION: 2.17mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 0.25mM Orotic Acid, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 155µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHVS SMIYSFYEPV TSVLFRYFSS GPLDPETAHG YTMELAKRGW LPVDYDREES ALNVDINGLK FLSPIGLAAG FDKHAEAPAA LLRMGFSFLE VGSITPKPQP GNPKPRLFRL YEDRSVINRF GFNSNGADYA QTQLEAFSEA RLRDPFTAQG VLGVSLGKNK TSEDAVADLR EGVKKLGRFA DFLVVNLSSP NTPGLRSLQS ASHLAAIIDG VQEELDALDR QAQAASQKQR NERRRHGGNP EETKAFYANQ TGRRPLFFVK IAPDLSMEEK ESIAKVALEK NLDGFVVSNT TIQRPETLKS PAKSETGGLS GRALKHLSTA CVSDMYKLTQ GKLAIIATGG VESGRDALDK IEAGASLVEL YSSMVYIGPQ VARRVKNELY HALNEKGYKD VAAAVGRKHK HVPEKKLQAP KFD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTTAGCA GCATGATCTA TAGCTTTTAT GAACCGGTTA CCAGCGTTCT GTTTCGTTAT TTCAGCAGCG GTCCGCTGGA CCCGGAAACC GCACATGGTT ATACCATGGA ACTGGCAAAA CGTGGTTGGC TGCCGGTTGA TTATGATCGT GAAGAAAGCG CACTGAACGT GGATATTAAT GGTCTGAAAT TTCTGAGCCC GATTGGTCTG GCAGCAGGTT TTGATAAACA TGCAGAAGCA CCGGCAGCAC TGCTGCGTAT GGGTTTTAGC TTTCTGGAAG TTGGTAGCAT TACACCGAAA CCGCAGCCTG GTAATCCGAA ACCTCGTCTG TTTCGCCTGT ATGAAGATCG TAGCGTTATT AATCGCTTTG GCTTTAATAG CAACGGTGCA GATTATGCAC AGACCCAGCT GGAAGCATTT AGCGAAGCAC GTCTGCGTGA TCCGTTTACC GCACAGGGTG TTCTGGGTGT TAGCCTGGGT AAAAACAAAA CCAGCGAAGA TGCAGTTGCC GATCTGCGTG AAGGTGTTAA AAAACTGGGT CGTTTTGCAG ATTTTCTGGT TGTTAATCTG AGCAGCCCGA ATACACCGGG TCTGCGTAGC CTGCAGAGCG CAAGCCATCT GGCAGCCATT ATTGATGGTG TTCAAGAGGA ACTGGATGCA CTGGATCGTC AGGCACAGGC AGCAAGCCAG AAACAGCGTA ATGAACGTCG TCGTCATGGT GGCAATCCGG AAGAAACCAA AGCATTTTAT GCAAATCAGA CCGGTCGTCG TCCGCTGTTT TTTGTTAAAA TTGCACCGGA TCTGAGCATG GAAGAAAAAG AAAGCATTGC AAAAGTGGCC CTGGAAAAAA ATCTGGATGG TTTTGTGGTT AGCAACACCA CCATTCAGCG TCCGGAAACA CTGAAAAGTC CGGCAAAAAG CGAAACCGGT GGTCTGAGCG GTCGTGCACT GAAACATCTG AGCACCGCAT GTGTTAGCGA TATGTATAAA CTGACCCAGG GTAAACTGGC AATTATTGCG ACCGGTGGTG TGGAAAGTGG TCGTGATGCC CTGGATAAAA TTGAAGCCGG TGCAAGCCTG GTTGAACTGT ATAGCAGTAT GGTTTATATT GGTCCGCAGG TTGCACGTCG TGTGAAAAAC GAACTGTATC ATGCACTGAA TGAGAAAGGC TATAAAGATG TTGCAGCAGC AGTTGGTCGT AAACATAAAC ATGTTCCGGA AAAAAAACTG CAGGCACCGA AATTTGATTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT
Details for TogoA.00487.a.B21.PS38564
PURIFICATION DATe: 3/27/2019
CONCENTRATION: 2.54mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 0.25mM Orotic Acid, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 120µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHVS SMIYSFYEPV TSVLFRYFSS GPLDPETAHG YTMELAKRGW LPVDYDREES ALNVDINGLK FLSPIGLAAG FDKHAEAPAA LLRMGFSFLE VGSITPKPQP GNPKPRLFRL YEDRSVINRF GFNSNGADYA QTQLEAFSEA RLRDPFTAQG VLGVSLGKNK TSEDAVADLR EGVKKLGRFA DFLVVNLSSP NTPGLRSLQS ASHLAAIIDG VQEELDALDR QAQAASQKQR NERRRHGGNP EETKAFYANQ TGRRPLFFVK IAPDLSMEEK ESIAKVALEK NLDGFVVSNT TIQRPETLKS PAKSETGGLS GRALKHLSTA CVSDMYKLTQ GKLAIIATGG VESGRDALDK IEAGASLVEL YSSMVYIGPQ VARRVKNELY HALNEKGYKD VAAAVGRKHK HVPEKKLQAP KFD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTTAGCA GCATGATCTA TAGCTTTTAT GAACCGGTTA CCAGCGTTCT GTTTCGTTAT TTCAGCAGCG GTCCGCTGGA CCCGGAAACC GCACATGGTT ATACCATGGA ACTGGCAAAA CGTGGTTGGC TGCCGGTTGA TTATGATCGT GAAGAAAGCG CACTGAACGT GGATATTAAT GGTCTGAAAT TTCTGAGCCC GATTGGTCTG GCAGCAGGTT TTGATAAACA TGCAGAAGCA CCGGCAGCAC TGCTGCGTAT GGGTTTTAGC TTTCTGGAAG TTGGTAGCAT TACACCGAAA CCGCAGCCTG GTAATCCGAA ACCTCGTCTG TTTCGCCTGT ATGAAGATCG TAGCGTTATT AATCGCTTTG GCTTTAATAG CAACGGTGCA GATTATGCAC AGACCCAGCT GGAAGCATTT AGCGAAGCAC GTCTGCGTGA TCCGTTTACC GCACAGGGTG TTCTGGGTGT TAGCCTGGGT AAAAACAAAA CCAGCGAAGA TGCAGTTGCC GATCTGCGTG AAGGTGTTAA AAAACTGGGT CGTTTTGCAG ATTTTCTGGT TGTTAATCTG AGCAGCCCGA ATACACCGGG TCTGCGTAGC CTGCAGAGCG CAAGCCATCT GGCAGCCATT ATTGATGGTG TTCAAGAGGA ACTGGATGCA CTGGATCGTC AGGCACAGGC AGCAAGCCAG AAACAGCGTA ATGAACGTCG TCGTCATGGT GGCAATCCGG AAGAAACCAA AGCATTTTAT GCAAATCAGA CCGGTCGTCG TCCGCTGTTT TTTGTTAAAA TTGCACCGGA TCTGAGCATG GAAGAAAAAG AAAGCATTGC AAAAGTGGCC CTGGAAAAAA ATCTGGATGG TTTTGTGGTT AGCAACACCA CCATTCAGCG TCCGGAAACA CTGAAAAGTC CGGCAAAAAG CGAAACCGGT GGTCTGAGCG GTCGTGCACT GAAACATCTG AGCACCGCAT GTGTTAGCGA TATGTATAAA CTGACCCAGG GTAAACTGGC AATTATTGCG ACCGGTGGTG TGGAAAGTGG TCGTGATGCC CTGGATAAAA TTGAAGCCGG TGCAAGCCTG GTTGAACTGT ATAGCAGTAT GGTTTATATT GGTCCGCAGG TTGCACGTCG TGTGAAAAAC GAACTGTATC ATGCACTGAA TGAGAAAGGC TATAAAGATG TTGCAGCAGC AGTTGGTCGT AAACATAAAC ATGTTCCGGA AAAAAAACTG CAGGCACCGA AATTTGATTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT