TogoA.00432.a

ras-related protein RAB5C, putative

CENTER ID: TogoA.00432.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.00432.a.A1.GE26590 Full length( TogoA.00432.a ) 1 243
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.00432.a.A1.PW26660 Full length( TogoA.00432.a ) 1 243

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_219720
RefSeq: XP_002370724.1
UniProt: B6KSA1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSFSQAYSSG ASLPPYGQSS GSGLGQSYAA GSSEAARPPS YKTVLLGDAS VGKSSLVVRF VKNTFSDTME TTIGAAFFTQ ALQVDGRTVK FEIWDTAGQE RFSSLAPMYY RGAAAAIVVY DQSNMASFDR AQVWVQQLQL SGNSNIVIAL AANKMDLPHK QVDLHLGRQY AEENGLLFIE TSAKTGQNVQ QLFSMIARRL PEKPVGAQGL GGGVHTIKLT DEPAVQRQAG GLASKFACCG GNS
NT Sequence
ATGTCTTTCT CGCAAGCTTA CAGTTCCGGA GCATCTCTTC CACCGTATGG CCAGAGCAGC GGCTCTGGTC TAGGCCAGTC TTACGCTGCG GGTTCCTCTG AGGCTGCGCG TCCGCCGTCG TACAAAACTG TGCTTCTAGG GGATGCCTCG GTGGGGAAGT CGAGTCTCGT GGTACGTTTT GTGAAAAACA CTTTTTCCGA CACGATGGAG ACGACGATCG GAGCTGCGTT CTTCACTCAG GCGCTCCAGG TTGACGGCCG AACAGTCAAG TTCGAAATCT GGGACACGGC GGGTCAGGAG CGGTTCTCCT CTCTGGCTCC CATGTACTAC AGAGGGGCAG CAGCCGCCAT TGTGGTGTAC GACCAGTCCA ACATGGCGAG CTTCGACCGC GCCCAAGTTT GGGTGCAGCA GCTCCAACTC AGCGGGAATT CGAACATCGT CATTGCGCTT GCAGCCAACA AGATGGACTT ACCACACAAA CAAGTCGACC TTCACCTCGG CCGCCAGTAC GCGGAAGAGA ACGGTCTCTT GTTCATCGAG ACCTCCGCGA AAACCGGCCA AAACGTCCAG CAACTCTTCT CCATGATCGC CAGACGCCTT CCCGAGAAAC CCGTCGGCGC CCAGGGCCTA GGCGGAGGCG TCCATACAAT CAAACTCACC GACGAACCCG CGGTCCAGAG GCAGGCAGGT GGACTCGCCA GCAAATTCGC CTGTTGCGGC GGAAACAGT
Details for TogoA.00432.a.A1.GE26590
HARVESTED ON: 4/23/2009
SEQUENCED ON: 5/21/2009
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSFSQAYSS GASLPPYGQS SGSGLGQSYA AGSSEAARPP SYKTVLLGDA SVGKSSLVVR FVKNTFSDTM ETTIGAAFFT QALQVDGRTV KFEIWDTAGQ ERFSSLAPMY YRGAAAAIVV YDQSNMASFD RAQVWVQQLQ LSGNSNIVIA LAANKMDLPH KQVDLHLGRQ YAEENGLLFI ETSAKTGQNV QQLFSMIARR LPEKPVGAQG LGGGVHTIKL TDEPAVQSQA GGLASKFACC GGNS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtctt tctcgcaagc ttacagttcc ggagcatctc ttccaccgta tggccagagc agcggctctg gtctaggcca gtcctacgct gcgggttcct ctgaggctgc gcgtccgccg tcgtacaaaa ctgtgcttct aggggatgcc tcggtgggga agtccagtct cgtggtacgt tttgtgaaaa acactttttc cgacacgatg gagacgacga tcggagctgc gttcttcact caggcgctcc aggttgacgg ccgaacagtc aagttcgaaa tctgggacac ggcgggtcag gagcggttct cctctctggc gcccatgtac tacagagggg cagcagccgc cattgtggtg tacgaccagt ccaacatggc gagcttcgac cgcgcccaag tttgggtgca gcagctccaa ctcagcggga attcgaacat cgtcattgcg cttgcagcca acaagatgga cttgccacac aaacaagtcg accttcacct cggccgccag tacgcggaag agaacggact cttgttcatc gagacctccg cgaaaaccgg ccaaaacgtc cagcaactct tctccatgat cgccagacgc cttcccgaga aacccgtcgg cgcccagggc ctaggcggag gcgtacatac aatcaaactc accgacgaac ccgcggtcca gagccaggca ggtggactcg ccagcaaatt cgcctgttgc ggcggaaaca gttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSFSQAYSS GASLPPYGQS SGSGLGQSYA AGSSEAARPP SYKTVLLGDA SVGKSSLVVR FVKNTFSDTM ETTIGAAFFT QALQVDGRTV KFEIWDTAGQ ERFSSLAPMY YRGAAAAIVV YDQSNMASFD RAQVWVQQLQ LSGNSNIVIA LAANKMDLPH KQVDLHLGRQ YAEENGLLFI ETSAKTGQNV QQLFSMIARR LPEKPVGAQG LGGGVHTIKL TDEPAVQRQA GGLASKFACC GGNS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctttctcg caagcttaca gttccggagc atctcttcca ccgtatggcc agagcagcgg ctctggtcta ggccagtctt acgctgcggg ttcctctgag gctgcgcgtc cgccgtcgta caaaactgtg cttctagggg atgcctcggt ggggaagtcg agtctcgtgg tacgttttgt gaaaaacact ttttccgaca cgatggagac gacgatcgga gctgcgttct tcactcaggc gctccaggtt gacggccgaa cagtcaagtt cgaaatctgg gacacggcgg gtcaggagcg gttctcctct ctggctccca tgtactacag aggggcagca gccgccattg tggtgtacga ccagtccaac atggcgagct tcgaccgcgc ccaagtttgg gtgcagcagc tccaactcag cgggaattcg aacatcgtca ttgcgcttgc agccaacaag atggacttac cacacaaaca agtcgacctt cacctcggcc gccagtacgc ggaagagaac ggtctcttgt tcatcgagac ctccgcgaaa accggccaaa acgtccagca actcttctcc atgatcgcca gacgccttcc cgagaaaccc gtcggcgccc agggcctagg cggaggcgtc catacaatca aactcaccga cgaacccgcg gtccagaggc aggcaggtgg actcgccagc aaattcgcct gttgcggcgg aaacagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for TogoA.00432.a.A1.PW26660
PURIFICATION DATe: 6/23/2009
CONCENTRATION: 28.09mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSFSQAYSS GASLPPYGQS SGSGLGQSYA AGSSEAARPP SYKTVLLGDA SVGKSSLVVR FVKNTFSDTM ETTIGAAFFT QALQVDGRTV KFEIWDTAGQ ERFSSLAPMY YRGAAAAIVV YDQSNMASFD RAQVWVQQLQ LSGNSNIVIA LAANKMDLPH KQVDLHLGRQ YAEENGLLFI ETSAKTGQNV QQLFSMIARR LPEKPVGAQG LGGGVHTIKL TDEPAVQSQA GGLASKFACC GGNS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtctt tctcgcaagc ttacagttcc ggagcatctc ttccaccgta tggccagagc agcggctctg gtctaggcca gtcctacgct gcgggttcct ctgaggctgc gcgtccgccg tcgtacaaaa ctgtgcttct aggggatgcc tcggtgggga agtccagtct cgtggtacgt tttgtgaaaa acactttttc cgacacgatg gagacgacga tcggagctgc gttcttcact caggcgctcc aggttgacgg ccgaacagtc aagttcgaaa tctgggacac ggcgggtcag gagcggttct cctctctggc gcccatgtac tacagagggg cagcagccgc cattgtggtg tacgaccagt ccaacatggc gagcttcgac cgcgcccaag tttgggtgca gcagctccaa ctcagcggga attcgaacat cgtcattgcg cttgcagcca acaagatgga cttgccacac aaacaagtcg accttcacct cggccgccag tacgcggaag agaacggact cttgttcatc gagacctccg cgaaaaccgg ccaaaacgtc cagcaactct tctccatgat cgccagacgc cttcccgaga aacccgtcgg cgcccagggc ctaggcggag gcgtacatac aatcaaactc accgacgaac ccgcggtcca gagccaggca ggtggactcg ccagcaaatt cgcctgttgc ggcggaaaca gttaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSFSQAYSS GASLPPYGQS SGSGLGQSYA AGSSEAARPP SYKTVLLGDA SVGKSSLVVR FVKNTFSDTM ETTIGAAFFT QALQVDGRTV KFEIWDTAGQ ERFSSLAPMY YRGAAAAIVV YDQSNMASFD RAQVWVQQLQ LSGNSNIVIA LAANKMDLPH KQVDLHLGRQ YAEENGLLFI ETSAKTGQNV QQLFSMIARR LPEKPVGAQG LGGGVHTIKL TDEPAVQRQA GGLASKFACC GGNS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTTTCTCG CAAGCTTACA GTTCCGGAGC ATCTCTTCCA CCGTATGGCC AGAGCAGCGG CTCTGGTCTA GGCCAGTCTT ACGCTGCGGG TTCCTCTGAG GCTGCGCGTC CGCCGTCGTA CAAAACTGTG CTTCTAGGGG ATGCCTCGGT GGGGAAGTCG AGTCTCGTGG TACGTTTTGT GAAAAACACT TTTTCCGACA CGATGGAGAC GACGATCGGA GCTGCGTTCT TCACTCAGGC GCTCCAGGTT GACGGCCGAA CAGTCAAGTT CGAAATCTGG GACACGGCGG GTCAGGAGCG GTTCTCCTCT CTGGCTCCCA TGTACTACAG AGGGGCAGCA GCCGCCATTG TGGTGTACGA CCAGTCCAAC ATGGCGAGCT TCGACCGCGC CCAAGTTTGG GTGCAGCAGC TCCAACTCAG CGGGAATTCG AACATCGTCA TTGCGCTTGC AGCCAACAAG ATGGACTTAC CACACAAACA AGTCGACCTT CACCTCGGCC GCCAGTACGC GGAAGAGAAC GGTCTCTTGT TCATCGAGAC CTCCGCGAAA ACCGGCCAAA ACGTCCAGCA ACTCTTCTCC ATGATCGCCA GACGCCTTCC CGAGAAACCC GTCGGCGCCC AGGGCCTAGG CGGAGGCGTC CATACAATCA AACTCACCGA CGAACCCGCG GTCCAGAGGC AGGCAGGTGG ACTCGCCAGC AAATTCGCCT GTTGCGGCGG AAACAGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA