TogoA.00248.a

elongation factor Tu, putative

CENTER ID: TogoA.00248.a
ORGANISM: Toxoplasma gondii ME49
ASSOCIATED DISEASE: Toxoplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TogoA.00248.a.A1.GE26587 Full length( TogoA.00248.a ) 1 401
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: ToxoDB:TGME49_302050
RefSeq: XP_002372087.1
UniProt: B6KW64

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKEIFKKQK PHINIGTIGH VDHGKTTLTA AITYVLAKNN QAKLKTYKEI DCAPEEIARG ITIKTSHIEY ETAVRHYAHI DCPGHADYIK NMITGAAQMD GAILVVSAVD GPMPQTKEHL LLAKQIGISN IIVFLNKIDL IDDNEILELV ELETRELLDK YNFSSDTPII TGSALKALDN NLTSNIWVDK IYELLTALDS YIPLPKRDLD KPFLLAIEDI FSITGRGTVV TGKIERGSIK LGDTVTMLGF NISKNVVVIG LEMFQKTLEI GEAGDNVGIL LRGIQKTEVK RGMILSKPLT MTLHSIFQAD VYILTVAEGG REKPIFEGYC PQFYLYTINI TGSIKFSSET KETGTKMILP GDRVKLNVTL IYSIAIEKGM RFAIREGGRT IGAGIITDII K
NT Sequence
ATGGCTAAAG AAATTTTTAA AAAACAAAAA CCTCATATAA ATATAGGAAC AATTGGACAT GTAGATCATG GAAAAACTAC TTTAACTGCT GCTATTACTT ATGTATTAGC TAAAAATAAT CAAGCAAAAC TTAAAACTTA CAAAGAAATT GATTGTGCTC CTGAAGAAAT AGCTAGAGGA ATTACAATTA AAACTTCACA TATTGAATAT GAAACTGCTG TAAGACATTA TGCTCATATT GATTGTCCTG GACATGCTGA TTATATTAAG AATATGATTA CTGGAGCCGC ACAAATGGAT GGAGCAATCT TAGTAGTATC TGCTGTAGAT GGGCCAATGC CACAAACTAA AGAACATTTA TTATTAGCTA AACAAATAGG TATTTCAAAT ATTATTGTAT TTCTAAATAA AATAGATTTA ATAGACGATA ATGAAATTTT AGAGTTAGTA GAATTAGAAA CTCGTGAATT ATTAGATAAA TATAATTTTT CATCAGATAC ACCTATTATT ACTGGTTCTG CTTTAAAAGC TTTAGATAAT AATTTAACAT CAAATATTTG GGTAGATAAA ATTTATGAAT TATTAACAGC TTTAGATTCA TATATTCCTT TACCAAAAAG AGATTTAGAT AAACCTTTTC TTTTAGCTAT TGAAGATATA TTTTCAATTA CAGGAAGAGG TACAGTAGTT ACTGGAAAAA TTGAAAGAGG TTCTATTAAA TTAGGCGATA CAGTAACAAT GTTAGGTTTT AATATTTCTA AAAATGTAGT AGTTATAGGA TTAGAAATGT TTCAAAAAAC CTTAGAAATA GGTGAAGCTG GAGACAATGT AGGTATTTTA TTAAGAGGTA TTCAAAAAAC TGAAGTAAAA CGTGGTATGA TTTTATCAAA ACCTTTAACT ATGACTCTTC ATTCTATATT TCAAGCAGAT GTATATATTT TAACTGTAGC TGAAGGTGGT AGAGAAAAAC CTATTTTTGA AGGATATTGC CCTCAATTTT ATTTATATAC GATAAATATT ACAGGTTCAA TAAAATTTTC TTCAGAAACA AAAGAAACTG GTACAAAAAT GATTCTTCCT GGAGATAGAG TTAAATTAAA TGTTACATTA ATTTATTCTA TTGCAATTGA AAAAGGTATG AGATTTGCTA TTAGAGAAGG AGGTCGTACT ATAGGAGCAG GTATAATTAC TGATATTATA AAA
Details for TogoA.00248.a.A1.GE26587
HARVESTED ON: 4/23/2009
SEQUENCED ON: 6/19/2009
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 73
Validated AA Sequence
MITGAAXMXG AILXVSAVXG PMPXXXXHLL LAKQIXISNI IVFLNKIDLI DDNEIXELVE LETRELLDKY NFSSDTPIIT GSALKALDNN LTSNIWVDKI YELLTALDSY IPLPKRDLDK PFLLAIEDIF SITGRGTVVT GKIERGSIKL GDTVTMLGFN ISKNVVVIGL EMFQKTLEIG EAGDNVGILL RGIQKTEVKR GMILSKPLTM TLHSIFQADV YILTVAEGGR EKPIFEGYCP QFYLYTINIT GSIKFSSETK ETGTKMILPG DRVKLNVTLI YSIAIEKGMR FAIREGGRTI GAGIITDIIK
Validated NT Sequence
attttataat atcagtaatt atacctgctc ctatagtacg acctccttct ctaatagcaa atctcatacc tttttcaatt gcaatagaat aaattaatgt aacatttaat ttaactctat ctccaggaag aatcattttt gtaccagttt cttttgtttc tgaagaaaat tttattgaac ctgtaatatt tatcgtatat aaataaaatt gagggcaata tccttcaaaa ataggttttt ctctaccacc ttcagctaca gttaaaatat atacatctgc ttgaaatata gaatgaagag tcatagttaa aggttttgat aaaatcatac cacgttttac ttcagttttt tgaatacctc ttaataaaat acctacattg tctccagctt cacctatttc taaggttttt tgaaacattt ctaatcctat aactactaca tttttagaaa tattaaaacc taacattgtt actgtatcgc ctaatttaat agaacctctt tcaatttttc cagtaactac tgtacctctt cctgtaattg aaaatatatc ttcaatagct aaaagaaaag gtttatctaa atctcttttt ggtaaaggaa tatatgaatc taaagctgtt aataattcat aaattttatc tacccaaata tttgatgtta aattattatc taaagctttt aaagcagaac cagtaataat aggtgtatct gatgaaaaat tatatttatc taataattca cgagtttcta attctactaa ctctnaaatt tcattatcgt ctattaaatc tattttattt agaaatacaa taatatttga aatacntatt tgtttagcta ataataaatg tnntntagnn ngtggcattg gcccatntac agcagatact antaagattg ctccntccat ttntgcggct ccagtaatca tattnttaat ataatcagca tgtccaggac aatcaatatg agcataatgt cttacagcag tttcatattc aatatgtgaa gttttaattg taattcctnn agntatttct tcaggagcac aatcaatttc tttgnnnntt ntaagttttg cttgattatt tttagnnaat acataagtaa tagcagcagt taaagtagtt tttccatgat ntacatgtnc aattgttcct atatttatan gaggtttntg ttttttaaaa ant
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAKEIFKKQ KPHINIGTIG HVDHGKTTLT AAITYVLAKN NQAKLKTYKE IDCAPEEIAR GITIKTSHIE YETAVRHYAH IDCPGHADYI KNMITGAAQM DGAILVVSAV DGPMPQTKEH LLLAKQIGIS NIIVFLNKID LIDDNEILEL VELETRELLD KYNFSSDTPI ITGSALKALD NNLTSNIWVD KIYELLTALD SYIPLPKRDL DKPFLLAIED IFSITGRGTV VTGKIERGSI KLGDTVTMLG FNISKNVVVI GLEMFQKTLE IGEAGDNVGI LLRGIQKTEV KRGMILSKPL TMTLHSIFQA DVYILTVAEG GREKPIFEGY CPQFYLYTIN ITGSIKFSSE TKETGTKMIL PGDRVKLNVT LIYSIAIEKG MRFAIREGGR TIGAGIITDI IK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctaaagaa atttttaaaa aacaaaaacc tcatataaat ataggaacaa ttggacatgt agatcatgga aaaactactt taactgctgc tattacttat gtattagcta aaaataatca agcaaaactt aaaacttaca aagaaattga ttgtgctcct gaagaaatag ctagaggaat tacaattaaa acttcacata ttgaatatga aactgctgta agacattatg ctcatattga ttgtcctgga catgctgatt atattaagaa tatgattact ggagccgcac aaatggatgg agcaatctta gtagtatctg ctgtagatgg gccaatgcca caaactaaag aacatttatt attagctaaa caaataggta tttcaaatat tattgtattt ctaaataaaa tagatttaat agacgataat gaaattttag agttagtaga attagaaact cgtgaattat tagataaata taatttttca tcagatacac ctattattac tggttctgct ttaaaagctt tagataataa tttaacatca aatatttggg tagataaaat ttatgaatta ttaacagctt tagattcata tattccttta ccaaaaagag atttagataa accttttctt ttagctattg aagatatatt ttcaattaca ggaagaggta cagtagttac tggaaaaatt gaaagaggtt ctattaaatt aggcgataca gtaacaatgt taggttttaa tatttctaaa aatgtagtag ttataggatt agaaatgttt caaaaaacct tagaaatagg tgaagctgga gacaatgtag gtattttatt aagaggtatt caaaaaactg aagtaaaacg tggtatgatt ttatcaaaac ctttaactat gactcttcat tctatatttc aagcagatgt atatatttta actgtagctg aaggtggtag agaaaaacct atttttgaag gatattgccc tcaattttat ttatatacga taaatattac aggttcaata aaattttctt cagaaacaaa agaaactggt acaaaaatga ttcttcctgg agatagagtt aaattaaatg ttacattaat ttattctatt gcaattgaaa aaggtatgag atttgctatt agagaaggag gtcgtactat aggagcaggt ataattactg atattataaa aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa