SSGCID: Target Page

TogoA.00246.a

leucine rich repeat protein, putative

CENTER ID:	TogoA.00246.a
ORGANISM:	Toxoplasma gondii ME49
ASSOCIATED DISEASE:	Toxoplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TogoA.00246.a.MB1.GE30404	Full length( TogoA.00246.a )		1	230

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	ToxoDB:TGME49_316420
RefSeq:	XP_002364809.1
UniProt:	B9PJD6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MWPQVLYLID NNLSDVTTIG YLPSLRGLYL QSNELTCTKG LEQLPCLTEL NLDDNKISRL EGLDSSTSLS RLSLTAQRTT TPLFLCPNVL TVLSRSLQYL NISKNRVEDL APFGTLQFLE VLQASENLVR EIGSIEAMLV GCRMLSTVDL RRNPVTKVRH YWERLLLGAS TTLKEADGRQ VTNASRFFVQ ELQKRRCAIM RQKTLDANDN LENSSNSQFP SRSDLATNSL

NT Sequence

ATGTGGCCCC AGGTGTTATA TCTCATCGAC AATAACCTTT CTGATGTAAC GACAATCGGG TACCTGCCTT CTTTACGAGG TCTTTACCTC CAGAGCAATG AGCTCACTTG TACGAAAGGC CTCGAGCAGC TGCCCTGTTT AACTGAGTTA AATCTCGATG ACAACAAGAT TTCCAGGCTG GAAGGTCTAG ACAGTTCCAC TAGCCTCTCC CGGTTGTCTC TGACAGCTCA AAGGACAACA ACACCGTTGT TTTTGTGTCC CAATGTCTTG ACTGTATTGA GTCGATCTCT GCAATACTTG AACATTTCCA AGAATAGAGT GGAGGATCTT GCACCATTTG GTACCCTGCA GTTTCTTGAA GTCCTCCAAG CATCAGAGAA CCTAGTTAGG GAAATCGGGT CGATTGAGGC TATGCTTGTC GGCTGCAGAA TGCTTTCCAC TGTGGATCTA CGACGCAACC CCGTCACTAA GGTGCGACAT TACTGGGAAC GTCTTCTCCT GGGAGCTTCT ACGACTCTGA AGGAGGCAGA CGGCCGCCAA GTTACTAATG CTAGCAGATT CTTCGTCCAG GAACTCCAGA AACGGCGATG CGCCATTATG AGGCAGAAGA CACTCGATGC GAATGATAAC TTGGAGAACT CATCTAACTC CCAGTTCCCT TCCAGAAGTG ATCTCGCAAC GAATTCCCTT

Details for TogoA.00246.a.MB1.GE30404

HARVESTED ON:	5/11/2010
SEQUENCED ON:	10/7/2010
EXPECTED MW:	70kDa
OBSERVED MW:	69kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	35

Validated AA Sequence

Validated NT Sequence

gnnctcagac ccngggtcct ggttcgatgt ggccccaggt gttatatctc atcgacaata acctttctga tgtaacgaca atcgggtacc tgccttcttt acgaggtctt tacctccaga gcaatgagct cacttgtacg aaaggcctcg agcagctgcc ctgtttaact gagttaaatc tcgatgacaa caagatttcc aggctggaag gtctagacag ttccactagc ctctcccggt tgtctctgac agctcaaagg acaacaacac cgttgttttt gtgtcccaat gtcttgactg tattgagtcg atctctgcaa tacttgaaca tttccaagaa tagagtggag gatcttgctc catttggtac cctgcagttt cttgaagtcc tccaagcatc agagaatcta gttagggaaa tcgggtcgat tgaggctatg cttgtcggct gcagaatgct ttccactgtg gatctacgac gcaaccccgt cactaaggtg cgacattact gggaacgtct tctcctggga gcttctacga ctctgaagga ggcagacggc cgccaagtta ctaatgctag cagattcttc gtccaggaac tccagaaacg gcgatgcgcc attatgaggc agaaaacact cgatgcgaat gataacttgg agaactcatc taactcccag ttcccttcca gaagtgatct cgcaacgaat tccctttaat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccnccg nnnnnnnnnn nnnnnna

Expected Protein Sequence

MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MWPQVLYLID NNLSDVTTIG YLPSLRGLYL QSNELTCTKG LEQLPCLTEL NLDDNKISRL EGLDSSTSLS RLSLTAQRTT TPLFLCPNVL TVLSRSLQYL NISKNRVEDL APFGTLQFLE VLQASENLVR EIGSIEAMLV GCRMLSTVDL RRNPVTKVRH YWERLLLGAS TTLKEADGRQ VTNASRFFVQ ELQKRRCAIM RQKTLDANDN LENSSNSQFP SRSDLATNSL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatgtg gccccaggtg ttatatctca tcgacaataa cctttctgat gtaacgacaa tcgggtacct gccttcttta cgaggtcttt acctccagag caatgagctc acttgtacga aaggcctcga gcagctgccc tgtttaactg agttaaatct cgatgacaac aagatttcca ggctggaagg tctagacagt tccactagcc tctcccggtt gtctctgaca gctcaaagga caacaacacc gttgtttttg tgtcccaatg tcttgactgt attgagtcga tctctgcaat acttgaacat ttccaagaat agagtggagg atcttgcacc atttggtacc ctgcagtttc ttgaagtcct ccaagcatca gagaacctag ttagggaaat cgggtcgatt gaggctatgc ttgtcggctg cagaatgctt tccactgtgg atctacgacg caaccccgtc actaaggtgc gacattactg ggaacgtctt ctcctgggag cttctacgac tctgaaggag gcagacggcc gccaagttac taatgctagc agattcttcg tccaggaact ccagaaacgg cgatgcgcca ttatgaggca gaagacactc gatgcgaatg ataacttgga gaactcatct aactcccagt tcccttccag aagtgatctc gcaacgaatt ccctttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa