StmaA.19928.a

Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) (bioA)

CENTER ID: StmaA.19928.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.19928.a.B1.GE43553 Full length(StmaA.19928.a) 1 435
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3445

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MREHPHTLPL VPIARGEGAW LIDHDGNRYL DAVSSWWTNL FGHAEPRIGG AIAAQAGQLE QVMLAGFGHE PAITLAERLL ALAPRQPGRE PLAKVFYADN GSAGVEVALK MAFQYFQNRG EPRRTRFIAL ENGYHGETLG ALAMADIPLY RRVYAPLLAE GLFAPSPDAY LAEPGQSAAD RARQAADGLA TLFDQHPGEI CAVILEPRLQ CAGGMRMHDP VYLQRVRELC DAHGAFMIAD EIATGFGRTG TLFACEQAGV MPDLMCLSKG LTGGFLPLAA VLATQALYDA FLDDSRERAF LHSHSYTGNP LACAAALATL DIFRDDDVIA RNRGIASVMG ALAAPLSDHP HVADVRQAGM VVAFELSRDG NKRTPFDPAL RLGLHAYKAA LKRGVVLRPL GDVLYWMPPY CVDDEQLELL AHTTLAAIDE AIACA
NT Sequence
ATGCGTGAGC ACCCGCATAC CCTGCCGCTG GTGCCGATCG CCCGTGGTGA AGGCGCATGG CTGATCGACC ACGACGGCAA CCGCTATCTG GACGCCGTCA GCAGCTGGTG GACCAATCTG TTCGGTCATG CCGAGCCGCG CATCGGCGGC GCCATCGCGG CGCAGGCCGG GCAGCTGGAA CAGGTGATGC TGGCTGGCTT CGGCCATGAG CCGGCGATCA CCCTGGCCGA GCGCCTGCTG GCGCTGGCGC CACGCCAGCC CGGCCGCGAG CCGCTGGCCA AGGTGTTCTA CGCCGACAAC GGCTCGGCCG GCGTTGAAGT CGCGCTGAAG ATGGCCTTCC AGTATTTCCA GAACCGCGGC GAGCCACGGC GCACGCGCTT CATCGCGCTG GAGAACGGTT ACCACGGCGA GACCCTTGGA GCGCTGGCGA TGGCGGACAT TCCGCTGTAC CGCCGCGTCT ACGCGCCGCT GCTGGCCGAA GGCCTGTTCG CGCCCTCGCC CGATGCCTAC CTGGCCGAGC CGGGCCAGAG CGCGGCTGAT CGCGCACGCC AGGCCGCTGA TGGCCTGGCC ACCCTGTTTG ACCAGCATCC CGGCGAGATC TGCGCAGTGA TCCTGGAACC GCGCCTGCAG TGCGCCGGCG GCATGCGCAT GCATGACCCG GTGTACCTGC AGCGGGTGCG CGAACTGTGT GATGCGCACG GCGCATTCAT GATCGCCGAC GAGATCGCCA CCGGCTTCGG CCGCACCGGC ACCCTGTTCG CCTGCGAGCA GGCCGGGGTG ATGCCGGATC TGATGTGCCT GTCCAAGGGC CTGACCGGCG GCTTCCTGCC GCTTGCCGCG GTGCTGGCCA CGCAGGCGCT GTACGACGCC TTCCTTGATG ACTCTCGCGA GCGCGCCTTC CTGCATTCGC ACAGTTACAC CGGCAACCCG CTGGCCTGTG CGGCTGCGCT GGCGACGCTG GACATCTTCC GCGACGACGA TGTGATCGCC CGCAACCGTG GCATCGCCTC GGTGATGGGC GCGCTGGCTG CGCCATTGAG CGACCATCCG CACGTGGCCG ACGTCCGCCA GGCCGGCATG GTGGTGGCCT TCGAGCTGTC GCGTGATGGC AACAAGCGCA CGCCGTTCGA TCCGGCGCTG CGACTGGGCC TGCACGCCTA CAAGGCTGCG CTGAAACGTG GCGTGGTGCT GCGTCCGCTG GGCGACGTGC TGTACTGGAT GCCACCCTAC TGCGTGGATG ACGAACAACT GGAGCTGCTG GCACATACCA CGCTGGCGGC GATTGACGAG GCGATTGCAT GCGCG
Details for StmaA.19928.a.B1.GE43553
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMR EHPHTLPLVP IARGEGAWLI DHDGNRYLDA VSSWWTNLFG HAEPRIGGAI AAQAGQLEQV MLAGFGHEPA ITLAERLLAL APRQPGREPL AKVFYADNGS AGVEVALKMA FQYFQNRGEP RRTRFIALEN GYHGETLGAL AMADIPLYRR VYAPLLAEGL FAPSPDAYLA EPGQSAADRA RQAADGLATL FDQHPGEICA VILEPRLQCA GGMRMHDPVY LQRVRELCDA HGAFMIADEI ATGFGRTGTL FACEQAGVMP DLMCLSKGXT GGFLPLAAVL ATQALYDAFL DDSRERAFLH SHSYTGNPLA CAAALXTLDI FRDDDVIARN RGIASVMGAL AAPLSDHPHV ADVRQAGMVV AFELSRDGNK RTPFDPALRL GLHAYKAALK RGVVLRPLGD VLYWMPPYCV DDEQLELLAH TTLAA
Validated NT Sequence
tcgccgccag cgtggtatgt gccagcagct ccagttgttc gtcatccacg cagtagggtg gcatccagta cagcacgtcg cccagcggac gcagcaccac gccacgtttc agcgcagcct tgtaggcgtg caggcccagt cgcagcgccg gatcgaacgg cgtgcgcttg ttgccatcac gcgacagctc gaaggccacc accatgccgg cctggcggac gtcggccacg tgcggatggt cgctcaatgg cgcagccagc gcgcccatca ccgaggcgat gccacggttg cgggcgatca catcgtcgtc gcggaagatg tccagcgtcn ccagcgcagc cgcacaggcc agcgggttgc cggtgtaact gtgcgaatgc aggaaggcgc gctcgcgaga gtcatcaagg aaggcgtcgt acagcgcctg cgtggccagc accgcggcaa gcggcaggaa gccgccggtc angcccttgg acaggcacat cagatccggc atcaccccgg cctgctcgca ggcgaacagg gtgccggtgc ggccgaagcc ggtggcgatc tcgtcggcga tcatgaatgc gccgtgcgca tcacacagtt cgcgcacccg ctgcaggtac accgggtcat gcatgcgcat gccgccggcg cactgcaggc gcggttccag gatcactgcg cagatctcgc cgggatgctg gtcaaacagg gtggccaggc catcagcggc ctggcgtgcg cgatcagccg cgctctggcc cggctcggcc aggtaggcat cgggcgaggg cgcgaacagg ccttcggcca gcagcggcgc gtagacgcgg cggtacagcg gaatgtccgc catcgccagc gctccaaggg tctcgccgtg gtaaccgttc tccagcgcga tgaagcgcgt gcgccgtggc tcgccgcggt tctggaaata ctggaaggcc atcttcagcg cgacttcaac gccggccgag ccgttgtcgg cgtagaacac cttggccagc ggctcgcggc cgggctggcg tggcgccagc gccagcaggc gctcggccag ggtgatcgcc ggctcatggc cgaagccagc cagcatcacc tgttccagct gcccggcctg cgccgcgatg gcgccgccga tgcgcggctc ggcatgaccg aacagattgg tccaccagct gctgacggcg tccagatagc ggttgccgtc gtggtcgatc agccatgcgc cttcaccacg ggcgatcggc accagcggca gggtatgcgg gtgctcacgc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMR EHPHTLPLVP IARGEGAWLI DHDGNRYLDA VSSWWTNLFG HAEPRIGGAI AAQAGQLEQV MLAGFGHEPA ITLAERLLAL APRQPGREPL AKVFYADNGS AGVEVALKMA FQYFQNRGEP RRTRFIALEN GYHGETLGAL AMADIPLYRR VYAPLLAEGL FAPSPDAYLA EPGQSAADRA RQAADGLATL FDQHPGEICA VILEPRLQCA GGMRMHDPVY LQRVRELCDA HGAFMIADEI ATGFGRTGTL FACEQAGVMP DLMCLSKGLT GGFLPLAAVL ATQALYDAFL DDSRERAFLH SHSYTGNPLA CAAALATLDI FRDDDVIARN RGIASVMGAL AAPLSDHPHV ADVRQAGMVV AFELSRDGNK RTPFDPALRL GLHAYKAALK RGVVLRPLGD VLYWMPPYCV DDEQLELLAH TTLAAIDEAI ACA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg agcacccgca taccctgccg ctggtgccga tcgcccgtgg tgaaggcgca tggctgatcg accacgacgg caaccgctat ctggacgccg tcagcagctg gtggaccaat ctgttcggtc atgccgagcc gcgcatcggc ggcgccatcg cggcgcaggc cgggcagctg gaacaggtga tgctggctgg cttcggccat gagccggcga tcaccctggc cgagcgcctg ctggcgctgg cgccacgcca gcccggccgc gagccgctgg ccaaggtgtt ctacgccgac aacggctcgg ccggcgttga agtcgcgctg aagatggcct tccagtattt ccagaaccgc ggcgagccac ggcgcacgcg cttcatcgcg ctggagaacg gttaccacgg cgagaccctt ggagcgctgg cgatggcgga cattccgctg taccgccgcg tctacgcgcc gctgctggcc gaaggcctgt tcgcgccctc gcccgatgcc tacctggccg agccgggcca gagcgcggct gatcgcgcac gccaggccgc tgatggcctg gccaccctgt ttgaccagca tcccggcgag atctgcgcag tgatcctgga accgcgcctg cagtgcgccg gcggcatgcg catgcatgac ccggtgtacc tgcagcgggt gcgcgaactg tgtgatgcgc acggcgcatt catgatcgcc gacgagatcg ccaccggctt cggccgcacc ggcaccctgt tcgcctgcga gcaggccggg gtgatgccgg atctgatgtg cctgtccaag ggcctgaccg gcggcttcct gccgcttgcc gcggtgctgg ccacgcaggc gctgtacgac gccttccttg atgactctcg cgagcgcgcc ttcctgcatt cgcacagtta caccggcaac ccgctggcct gtgcggctgc gctggcgacg ctggacatct tccgcgacga cgatgtgatc gcccgcaacc gtggcatcgc ctcggtgatg ggcgcgctgg ctgcgccatt gagcgaccat ccgcacgtgg ccgacgtccg ccaggccggc atggtggtgg ccttcgagct gtcgcgtgat ggcaacaagc gcacgccgtt cgatccggcg ctgcgactgg gcctgcacgc ctacaaggct gcgctgaaac gtggcgtggt gctgcgtccg ctgggcgacg tgctgtactg gatgccaccc tactgcgtgg atgacgaaca actggagctg ctggcacata ccacgctggc ggcgattgac gaggcgattg catgcgcgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat